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Home / Equine Research Bank / Nucleic Acids Res / Evolution of the herpes thymidine kinase: identification and...
Nucleic acids research1988; 16(23); 11303-11317; doi: 10.1093/nar/16.23.11303

Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase.

Abstract: We have identified the equine herpesvirus 1 (EHV-1) thymidine kinase gene (TK) by DNA-mediated transformation and by DNA sequencing. Alignment of the amino acid sequence of the EHV-1 TK with the TKs from 3 other herpesviruses revealed regions of homology, some of which correspond to the previously identified substrate binding sites, while others have as yet, no assigned function. In particular, the strict conservation of an aspartate within the proposed nucleoside binding site suggests a role in ATP binding for this residue. Comparison of 5 herpes TKs with the thymidylate kinase of yeast revealed significant similarity which was strongest in those regions important to catalytic activity of the herpes TKs, and, therefore we propose that the herpes TK may be derived from a cellular thymidylate kinase. The implications for the evolution of enzyme activities within a pathway of nucleotide metabolism are discussed.
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Publication Date: 1988-12-09 PubMed ID: 2849761PubMed Central: PMC339011DOI: 10.1093/nar/16.23.11303Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the identification and comparison of a thymidine kinase gene found in the equine herpesvirus, uncovering similarities between this gene and a cell-encoded thymidylate kinase.

Identification of the EHV-1 Thymidine Kinase Gene

  • The study identified the equine herpesvirus 1 (EHV-1) thymidine kinase gene (TK). This was done through DNA sequencing and DNA-mediated transformation, a process where foreign DNA is introduced to a cell, triggering a genotype change.
  • EHV-1 is a DNA virus that can cause respiratory diseases, neurological disorders, and abortion in horses.

Homology with other Herpesviruses

  • The researchers compared the amino acid sequence of EHV-1’s TK gene with those from three other herpesviruses.
  • They discovered regions of homology, indicating similar sequences of amino acids. Some of these regions match previously identified substrate-binding sites.
  • There are also some areas of homology between these genes that currently have no assigned function.

Role of Aspartate Residue

  • The researchers noted a specific aspartate residue that was strictly conserved in the nucleoside binding site. This suggests it may have a role in binding adenosine triphosphate (ATP), an important molecule in providing cellular energy.

Similarity with Yeast’s Thymidylate Kinase

  • The TK genes from five herpesviruses were compared with the thymidylate kinase gene of yeast. Significant similarities were found, particularly in those regions crucial to the catalytic activity of the TK genes.
  • The researchers propose based on this observation, that the herpes virus TK might have evolved from a cellular thymidylate kinase, suggesting the virus may have originated from a living cell at some point in its evolution.

Implications for Nucleotide Metabolism

  • The study discusses the implications of these findings for the evolution of enzyme activities within the pathway of nucleotide metabolism – the process by which nucleotides (building blocks of nucleic acids such as DNA and RNA) are synthesized and broken down.

Cite This Article

APA
Robertson GR, Whalley JM. (1988). Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase. Nucleic Acids Res, 16(23), 11303-11317. https://doi.org/10.1093/nar/16.23.11303

Publication

Nucleic acids research
ISSN: 0305-1048
NlmUniqueID: 0411011
Country: England
Language: English
Volume: 16
Issue: 23
Pages: 11303-11317

Researcher Affiliations

Robertson, G R
  • School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Whalley, J M

    MeSH Terms

    • Amino Acid Sequence
    • Animals
    • Base Sequence
    • Cell Line, Transformed
    • Genes
    • Herpesviridae / enzymology
    • Herpesvirus 1, Equid / enzymology
    • Herpesvirus 1, Equid / genetics
    • Mice
    • Molecular Sequence Data
    • Nucleoside-Phosphate Kinase / genetics
    • Phosphotransferases / genetics
    • Saccharomyces cerevisiae / enzymology
    • Sequence Homology, Nucleic Acid
    • Thymidine Kinase / genetics
    • Thymidine Kinase / isolation & purification

    References

    This article includes 55 references
    1. Dale RM, McClure BA, Houchins JP. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.. Plasmid 1985 Jan;13(1):31-40.
      pubmed: 3991809doi: 10.1016/0147-619x(85)90053-8google scholar: lookup
    2. Jong AY, Kuo CL, Campbell JL. The CDC8 gene of yeast encodes thymidylate kinase.. J Biol Chem 1984 Sep 10;259(17):11052-9.
      pubmed: 6088527
    3. Fry DC, Kuby SA, Mildvan AS. ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins.. Proc Natl Acad Sci U S A 1986 Feb;83(4):907-11.
      pubmed: 2869483doi: 10.1073/pnas.83.4.907google scholar: lookup
    4. McGeoch DJ, Davison AJ. Alphaherpesviruses possess a gene homologous to the protein kinase gene family of eukaryotes and retroviruses.. Nucleic Acids Res 1986 Feb 25;14(4):1765-77.
      pubmed: 3005981doi: 10.1093/nar/14.4.1765google scholar: lookup
    5. McKnight S, Tjian R. Transcriptional selectivity of viral genes in mammalian cells.. Cell 1986 Sep 12;46(6):795-805.
      pubmed: 3530495doi: 10.1016/0092-8674(86)90061-9google scholar: lookup
    6. Leader DP, Katan M. Viral aspects of protein phosphorylation.. J Gen Virol 1988 Jul;69 ( Pt 7):1441-64.
      pubmed: 2455769doi: 10.1099/0022-1317-69-7-1441google scholar: lookup
    7. Graham D, Larder BA, Inglis MM. Evidence that the 'active centre' of the herpes simplex virus thymidine kinase involves an interaction between three distinct regions of the polypeptide.. J Gen Virol 1986 Apr;67 ( Pt 4):753-8.
      pubmed: 3007662doi: 10.1099/0022-1317-67-4-753google scholar: lookup
    8. Allen GP, McGowan JJ, Gentry GA, Randall CC. Biochemical transformation of deoxythymidine kinase-deficient mouse cells with UV-irradiated equine herpesvirus type 1.. J Virol 1978 Oct;28(1):361-7.
      pubmed: 212607doi: 10.1128/JVI.28.1.361-367.1978google scholar: lookup
    9. Kit S. Thymidine kinase.. Microbiol Sci 1985 Dec;2(12):369-75.
      pubmed: 3939993
    10. Fristensky B, Lis J, Wu R. Portable microcomputer software for nucleotide sequence analysis.. Nucleic Acids Res 1982 Oct 25;10(20):6451-63.
      pubmed: 6184674doi: 10.1093/nar/10.20.6451google scholar: lookup
    11. Breen EJ, Browne LH, Glue L, Williams KL. The DNA rodent: a portable hand held DNA sequence reader.. Comput Appl Biosci 1988 Mar;4(1):217.
      pubmed: 3383000doi: 10.1093/bioinformatics/4.1.217google scholar: lookup
    12. Kit S, Ichimura H, De Clercq E. Phosphorylation of nucleoside analogs by equine herpesvirus type 1 pyrimidine deoxyribonucleoside kinase.. Antiviral Res 1987 Jan;7(1):53-67.
      pubmed: 3026247doi: 10.1016/0166-3542(87)90039-8google scholar: lookup
    13. Inglis MM, Darby G. Analysis of the role of the cysteine 171 residue in the activity of herpes simplex virus type 1 thymidine kinase by oligonucleotide-directed mutagenesis.. J Gen Virol 1987 Jan;68 ( Pt 1):39-46.
      pubmed: 3027247doi: 10.1099/0022-1317-68-1-39google scholar: lookup
    14. Walker JE, Saraste M, Runswick MJ, Gay NJ. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.. EMBO J 1982;1(8):945-51.
      pubmed: 6329717doi: 10.1002/j.1460-2075.1982.tb01276.xgoogle scholar: lookup
    15. Gentry GA. Locating a nucleotide-binding site in the thymidine kinase of vaccinia virus and of herpes simplex virus by scoring triply aligned protein sequences.. Proc Natl Acad Sci U S A 1985 Oct;82(20):6815-9.
      pubmed: 2995987doi: 10.1073/pnas.82.20.6815google scholar: lookup
    16. Horowitz NH. On the Evolution of Biochemical Syntheses.. Proc Natl Acad Sci U S A 1945 Jun;31(6):153-7.
      pubmed: 16578152doi: 10.1073/pnas.31.6.153google scholar: lookup
    17. Jamieson AT, Subak-Sharpe JH. Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity.. J Gen Virol 1974 Sep;24(3):481-92.
      pubmed: 4372300doi: 10.1099/0022-1317-24-3-481google scholar: lookup
    18. Chen MS, Walker J, Prusoff WH. Kinetic studies of herpes simplex virus type 1-encoded thymidine and thymidylate kinase, a multifunctional enzyme.. J Biol Chem 1979 Nov 10;254(21):10747-53.
      pubmed: 227850
    19. Allen GP, McGowan JJ, Randall CC, Mancini W, Cheng YC, Gentry GA. Purification and characterization of deoxythymidine kinase (dTK) induced in dTK- 3T3 mouse cells by equine herpesvirus type 1 (EHV-1).. Virology 1979 Jan 30;92(2):367-74.
      pubmed: 218350doi: 10.1016/0042-6822(79)90141-7google scholar: lookup
    20. Whalley JM, Robertson GR, Davison AJ. Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, BglII and BamHI.. J Gen Virol 1981 Dec;57(Pt 2):307-23.
      pubmed: 6275028doi: 10.1099/0022-1317-57-2-307google scholar: lookup
    21. Haarr L, Flatmark T. Evidence that deletion of coding sequences in the 5' end of the thymidine kinase gene of herpes simplex virus type 1 affects the stability of the gene products.. J Gen Virol 1987 Nov;68 ( Pt 11):2817-29.
      pubmed: 2824663doi: 10.1099/0022-1317-68-11-2817google scholar: lookup
    22. Pai EF, Sachsenheimer W, Schirmer RH, Schulz GE. Substrate positions and induced-fit in crystalline adenylate kinase.. J Mol Biol 1977 Jul;114(1):37-45.
      pubmed: 198550doi: 10.1016/0022-2836(77)90281-9google scholar: lookup
    23. Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches.. Science 1985 Mar 22;227(4693):1435-41.
      pubmed: 2983426doi: 10.1126/science.2983426google scholar: lookup
    24. Davison AJ, Wilkie NM. Location and orientation of homologous sequences in the genomes of five herpesviruses.. J Gen Virol 1983 Sep;64 (Pt 9):1927-42.
      pubmed: 6310033doi: 10.1099/0022-1317-64-9-1927google scholar: lookup
    25. Dever TE, Glynias MJ, Merrick WC. GTP-binding domain: three consensus sequence elements with distinct spacing.. Proc Natl Acad Sci U S A 1987 Apr;84(7):1814-8.
      pubmed: 3104905doi: 10.1073/pnas.84.7.1814google scholar: lookup
    26. Shoji S, Parmelee DC, Wade RD, Kumar S, Ericsson LH, Walsh KA, Neurath H, Long GL, Demaille JG, Fischer EH, Titani K. Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase.. Proc Natl Acad Sci U S A 1981 Feb;78(2):848-51.
      pubmed: 6262777doi: 10.1073/pnas.78.2.848google scholar: lookup
    27. Gompels U, Minson A. The properties and sequence of glycoprotein H of herpes simplex virus type 1.. Virology 1986 Sep;153(2):230-47.
      pubmed: 3016991doi: 10.1016/0042-6822(86)90026-7google scholar: lookup
    28. Allen GP, McGowan JJ, Bryans JT, Randall CC, Gentry GA. Induction of deoxythymidine kinase activity in several mammalian cell lines after infection with six different strains of equine herpesvirus type 1 (EHV-1).. Virology 1978 Oct 15;90(2):351-9.
      pubmed: 214953doi: 10.1016/0042-6822(78)90319-7google scholar: lookup
    29. Liu QY, Summers WC. Site-directed mutagenesis of a nucleotide-binding domain in HSV-1 thymidine kinase: effects on catalytic activity.. Virology 1988 Apr;163(2):638-42.
      pubmed: 2833027doi: 10.1016/0042-6822(88)90308-xgoogle scholar: lookup
    30. Otsuka H, Kit S. Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide.. Virology 1984 Jun;135(2):316-30.
      pubmed: 6330976doi: 10.1016/0042-6822(84)90189-2google scholar: lookup
    31. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci U S A 1977 Dec;74(12):5463-7.
      pubmed: 271968doi: 10.1073/pnas.74.12.5463google scholar: lookup
    32. Bello LJ, Whitbeck JC, Lawrence WC. Map location of the thymidine kinase gene of bovine herpesvirus 1.. J Virol 1987 Dec;61(12):4023-5.
      pubmed: 2824831doi: 10.1128/JVI.61.12.4023-4025.1987google scholar: lookup
    33. Chen MS, Prusoff WH. Association of thymidylate kinase activity with pyrimidine deoxyribonucleoside kinase induced by herpes simplex virus.. J Biol Chem 1978 Mar 10;253(5):1325-7.
      pubmed: 203589
    34. McGeoch DJ, Dalrymple MA, Dolan A, McNab D, Perry LJ, Taylor P, Challberg MD. Structures of herpes simplex virus type 1 genes required for replication of virus DNA.. J Virol 1988 Feb;62(2):444-53.
      pubmed: 2826807doi: 10.1128/JVI.62.2.444-453.1988google scholar: lookup
    35. Kolb E, Hudson PJ, Harris JI. Phosphofructokinase: complete amino-acid sequence of the enzyme from Bacillus stearothermophilus.. Eur J Biochem 1980 Jul;108(2):587-97.
      pubmed: 6447595doi: 10.1111/j.1432-1033.1980.tb04754.xgoogle scholar: lookup
    36. Kwoh TJ, Engler JA. The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase.. Nucleic Acids Res 1984 May 11;12(9):3959-71.
      pubmed: 6328447doi: 10.1093/nar/12.9.3959google scholar: lookup
    37. Cohen GH. Ribonucleotide reductase activity of synchronized KB cells infected with herpes simplex virus.. J Virol 1972 Mar;9(3):408-18.
      pubmed: 4335659doi: 10.1128/JVI.9.3.408-418.1972google scholar: lookup
    38. Takio K, Wade RD, Smith SB, Krebs EG, Walsh KA, Titani K. Guanosine cyclic 3',5'-phosphate dependent protein kinase, a chimeric protein homologous with two separate protein families.. Biochemistry 1984 Aug 28;23(18):4207-18.
      pubmed: 6091741doi: 10.1021/bi00313a030google scholar: lookup
    39. Henry BE, Glaser R, Hewetson J, O'Callaghan DJ. Expression of altered ribonucleotide reductase activity associated with the replication of the Epstein-Barr virus.. Virology 1978 Aug;89(1):262-71.
      pubmed: 210572doi: 10.1016/0042-6822(78)90058-2google scholar: lookup
    40. Michelson S, Tardy-Panit M, Bârzu O. Properties of a human cytomegalovirus-induced protein kinase.. Virology 1984 Apr 30;134(2):259-68.
      pubmed: 6100570doi: 10.1016/0042-6822(84)90295-2google scholar: lookup
    41. Crute JJ, Mocarski ES, Lehman IR. A DNA helicase induced by herpes simplex virus type 1.. Nucleic Acids Res 1988 Jul 25;16(14A):6585-96.
      pubmed: 2840645doi: 10.1093/nar/16.14.6585google scholar: lookup
    42. Baer R, Bankier AT, Biggin MD, Deininger PL, Farrell PJ, Gibson TJ, Hatfull G, Hudson GS, Satchwell SC, Séguin C. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.. Nature 1984 Jul 19-25;310(5974):207-11.
      pubmed: 6087149doi: 10.1038/310207a0google scholar: lookup
    43. Morrison JM, Keir HM. A new DNA-exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme.. J Gen Virol 1968 Dec;3(3):337-47.
      pubmed: 4304099doi: 10.1099/0022-1317-3-3-337google scholar: lookup
    44. Thompson R, Honess RW, Taylor L, Morran J, Davison AJ. Varicella-zoster virus specifies a thymidylate synthetase.. J Gen Virol 1987 May;68 ( Pt 5):1449-55.
      pubmed: 3033144doi: 10.1099/0022-1317-68-5-1449google scholar: lookup
    45. Davison AJ, Scott JE. The complete DNA sequence of varicella-zoster virus.. J Gen Virol 1986 Sep;67 ( Pt 9):1759-816.
      pubmed: 3018124doi: 10.1099/0022-1317-67-9-1759google scholar: lookup
    46. Fyfe JA, McKee SA, Keller PM. Altered thymidine-thymidylate kinases from strains of herpes simplex virus with modified drug sensitivities to acyclovir and (E)-5-(2-bromovinyl)-2'-deoxyuridine.. Mol Pharmacol 1983 Sep;24(2):316-23.
      pubmed: 6310366
    47. Bodemer W, Niller HH, Nitsche N, Scholz B, Fleckenstein B. Organization of the thymidylate synthase gene of herpesvirus saimiri.. J Virol 1986 Oct;60(1):114-23.
      pubmed: 3018278doi: 10.1128/JVI.60.1.114-123.1986google scholar: lookup
    48. Boyle DB, Coupar BE, Gibbs AJ, Seigman LJ, Both GW. Fowlpox virus thymidine kinase: nucleotide sequence and relationships to other thymidine kinases.. Virology 1987 Feb;156(2):355-65.
      pubmed: 3027984doi: 10.1016/0042-6822(87)90415-6google scholar: lookup
    49. Chen MS, Summers WP, Walker J, Summers WC, Prusoff WH. Characterization of pyrimidine deoxyribonucleoside kinase (thymidine kinase) and thymidylate kinase as a multifunctional enzyme in cells transformed by herpes simplex virus type 1 and in cells infected with mutant strains of herpes simplex virus.. J Virol 1979 Jun;30(3):942-5.
      pubmed: 225551doi: 10.1128/JVI.30.3.942-945.1979google scholar: lookup
    50. Halpern ME, Smiley JR. Effects of deletions on expression of the herpes simplex virus thymidine kinase gene from the intact viral genome: the amino terminus of the enzyme is dispensable for catalytic activity.. J Virol 1984 Jun;50(3):733-8.
      pubmed: 6328003doi: 10.1128/JVI.50.3.733-738.1984google scholar: lookup
    51. Littler E, Zeuthen J, McBride AA, Trøst Sørensen E, Powell KL, Walsh-Arrand JE, Arrand JR. Identification of an Epstein-Barr virus-coded thymidine kinase.. EMBO J 1986 Aug;5(8):1959-66.
      pubmed: 3019675doi: 10.1002/j.1460-2075.1986.tb04450.xgoogle scholar: lookup
    52. Swain MA, Galloway DA. Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene.. J Virol 1983 Jun;46(3):1045-50.
      pubmed: 6304336doi: 10.1128/JVI.46.3.1045-1050.1983google scholar: lookup
    53. McKnight SL. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.. Nucleic Acids Res 1980 Dec 20;8(24):5949-64.
      pubmed: 6258156doi: 10.1093/nar/8.24.5949google scholar: lookup
    54. Wagner MJ, Sharp JA, Summers WC. Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.. Proc Natl Acad Sci U S A 1981 Mar;78(3):1441-5.
      pubmed: 6262799doi: 10.1073/pnas.78.3.1441google scholar: lookup
    55. Graham FL, van der Eb AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA.. Virology 1973 Apr;52(2):456-67.
      pubmed: 4705382doi: 10.1016/0042-6822(73)90341-3google scholar: lookup

    Citations

    This article has been cited 26 times.
    1. Xie Y, Wu L, Wang M, Cheng A, Yang Q, Wu Y, Jia R, Zhu D, Zhao X, Chen S, Liu M, Zhang S, Wang Y, Xu Z, Chen Z, Zhu L, Luo Q, Liu Y, Yu Y, Zhang L, Chen X. Alpha-Herpesvirus Thymidine Kinase Genes Mediate Viral Virulence and Are Potential Therapeutic Targets.. Front Microbiol 2019;10:941.
      doi: 10.3389/fmicb.2019.00941pubmed: 31134006google scholar: lookup
    2. Li H, Liu S, Kong X. Characterization of the genes encoding UL24, TK and gH proteins from duck enteritis virus (DEV): a proof for the classification of DEV.. Virus Genes 2006 Oct;33(2):221-7.
      doi: 10.1007/s11262-005-0060-6pubmed: 16972038google scholar: lookup
    3. Cannon JS, Hamzeh F, Moore S, Nicholas J, Ambinder RF. Human herpesvirus 8-encoded thymidine kinase and phosphotransferase homologues confer sensitivity to ganciclovir.. J Virol 1999 Jun;73(6):4786-93.
      doi: 10.1128/JVI.73.6.4786-4793.1999pubmed: 10233939google scholar: lookup
    4. Moore DM, Zsak L, Neilan JG, Lu Z, Rock DL. The African swine fever virus thymidine kinase gene is required for efficient replication in swine macrophages and for virulence in swine.. J Virol 1998 Dec;72(12):10310-5.
      doi: 10.1128/JVI.72.12.10310-10315.1998pubmed: 9811782google scholar: lookup
    5. Chen SH, Cook WJ, Grove KL, Coen DM. Human thymidine kinase can functionally replace herpes simplex virus type 1 thymidine kinase for viral replication in mouse sensory ganglia and reactivation from latency upon explant.. J Virol 1998 Aug;72(8):6710-5.
      doi: 10.1128/JVI.72.8.6710-6715.1998pubmed: 9658118google scholar: lookup
    6. Shimojima Y, Jang HK, Ono M, Maeda K, Tohya Y, Mikami T. Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the thymidine kinase and UL24 genes of herpes simplex virus type 1.. Virus Genes 1997;14(1):81-7.
      doi: 10.1023/a:1007943624997pubmed: 9208458google scholar: lookup
    7. Kim SK, Holden VR, O'Callaghan DJ. The ICP22 protein of equine herpesvirus 1 cooperates with the IE protein to regulate viral gene expression.. J Virol 1997 Feb;71(2):1004-12.
      doi: 10.1128/JVI.71.2.1004-1012.1997pubmed: 8995619google scholar: lookup
    8. Xuan X, Maeda K, Tohya Y, Mikami T, Otsuka H. Identification and nucleotide sequence of the thymidine kinase gene of canine herpesvirus.. Virus Genes 1996;12(2):185-8.
      doi: 10.1007/BF00572957pubmed: 8879135google scholar: lookup
    9. Reynes JP, Tiraby M, Baron M, Drocourt D, Tiraby G. Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus.. J Bacteriol 1996 May;178(10):2804-12.
      doi: 10.1128/jb.178.10.2804-2812.1996pubmed: 8631667google scholar: lookup
    10. Pumphrey CY, Gray WL. Identification and analysis of the simian varicella virus thymidine kinase gene.. Arch Virol 1996;141(1):43-55.
      doi: 10.1007/BF01718587pubmed: 8629950google scholar: lookup
    11. Talarico CL, Phelps WC, Biron KK. Analysis of the thymidine kinase genes from acyclovir-resistant mutants of varicella-zoster virus isolated from patients with AIDS.. J Virol 1993 Feb;67(2):1024-33.
      doi: 10.1128/JVI.67.2.1024-1033.1993pubmed: 8380452google scholar: lookup
    12. Nagesha HS, Crabb BS, Studdert MJ. Analysis of the nucleotide sequence of five genes at the left end of the unique short region of the equine herpesvirus 4 genome.. Arch Virol 1993;128(1-2):143-54.
      doi: 10.1007/BF01309795pubmed: 8380320google scholar: lookup
    13. Koonin EV, Senkevich TG. Fowlpox virus encodes a protein related to human deoxycytidine kinase: further evidence for independent acquisition of genes for enzymes of nucleotide metabolism by different viruses.. Virus Genes 1993 Sep;7(3):289-95.
      doi: 10.1007/BF01702589pubmed: 8279127google scholar: lookup
    14. Bell CW, Whalley JM. Herpesvirus ICP18.5 and DNA-binding protein genes are conserved in equine herpesvirus-1.. Virus Genes 1993 Sep;7(3):219-28.
      doi: 10.1007/BF01702583pubmed: 8279122google scholar: lookup
    15. Nunberg JH, Wright DK, Cole GE, Petrovskis EA, Post LE, Compton T, Gilbert JH. Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpesvirus gene homologs.. J Virol 1989 Aug;63(8):3240-9.
      doi: 10.1128/JVI.63.8.3240-3249.1989pubmed: 2746729google scholar: lookup
    16. Gorbalenya AE, Koonin EV. Viral proteins containing the purine NTP-binding sequence pattern.. Nucleic Acids Res 1989 Nov 11;17(21):8413-40.
      doi: 10.1093/nar/17.21.8413pubmed: 2555771google scholar: lookup
    17. Smith GL, de Carlos A, Chan YS. Vaccinia virus encodes a thymidylate kinase gene: sequence and transcriptional mapping.. Nucleic Acids Res 1989 Oct 11;17(19):7581-90.
      doi: 10.1093/nar/17.19.7581pubmed: 2552411google scholar: lookup
    18. Blasco R, López-Otín C, Muñóz M, Bockamp EO, Simón-Mateo C, Viñuela E. Sequence and evolutionary relationships of African swine fever virus thymidine kinase.. Virology 1990 Sep;178(1):301-4.
      doi: 10.1016/0042-6822(90)90409-kpubmed: 2389555google scholar: lookup
    19. Su JY, Sclafani RA. Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast.. Nucleic Acids Res 1991 Feb 25;19(4):823-7.
      doi: 10.1093/nar/19.4.823pubmed: 2017365google scholar: lookup
    20. Smith GA, Young PL, Mattick JS. Nucleotide and amino acid sequence analysis of the thymidine kinase gene of a bovine encephalitis herpesvirus.. Arch Virol 1991;119(3-4):199-210.
      doi: 10.1007/BF01310670pubmed: 1678935google scholar: lookup
    21. Whalley M, Robertson G, Bell C, Love D, Elphinstone M, Wiley L, Craven D. Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene.. Virus Genes 1991 Oct;5(4):313-25.
      doi: 10.1007/BF00271530pubmed: 1665613google scholar: lookup
    22. Folkers G, Trumpp-Kallmeyer S, Gutbrod O, Krickl S, Fetzer J, Keil GM. Computer-aided active-site-directed modeling of the herpes simplex virus 1 and human thymidine kinase.. J Comput Aided Mol Des 1991 Oct;5(5):385-404.
      doi: 10.1007/BF00125660pubmed: 1663155google scholar: lookup
    23. Lomonte P, Bublot M, Pastoret PP, Thiry E. Location and characterization of the bovine herpesvirus type 4 thymidine kinase gene; comparison with thymidine kinase genes of other herpesviruses.. Arch Virol 1992;127(1-4):327-37.
      doi: 10.1007/BF01309595pubmed: 1339265google scholar: lookup
    24. Koonin EV, Senkevich TG. Evolution of thymidine and thymidylate kinases: the possibility of independent capture of TK genes by different groups of viruses.. Virus Genes 1992 Apr;6(2):187-96.
      doi: 10.1007/BF01703067pubmed: 1317076google scholar: lookup
    25. Suzutani T, Lacey SF, Powell KL, Purifoy DJ, Honess RW. Random mutagenesis of the thymidine kinase gene of varicella-zoster virus.. J Virol 1992 Apr;66(4):2118-24.
      doi: 10.1128/JVI.66.4.2118-2124.1992pubmed: 1312622google scholar: lookup
    26. Smith RH, Caughman GB, O'Callaghan DJ. Characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product.. J Virol 1992 Feb;66(2):936-45.
      doi: 10.1128/JVI.66.2.936-945.1992pubmed: 1309921google scholar: lookup
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