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European journal of applied physiology2004; 93(4); 496-501; doi: 10.1007/s00421-004-1162-x

Exercise-induced oxidative stress and muscle stress protein responses in trotters.

Abstract: Acute exercise induces oxidative stress and heat shock protein (HSP) expression. Information on the protection of stress proteins against oxidant insult and muscle damage during moderate exercise is scanty. We aimed to show how a single bout of moderate exercise affects the markers of oxidative stress and heat shock factor-1 (HSF1; the transcriptional regulator of HSP synthesis), and HSP70, HSP90 and glucose-regulated protein (GRP75) expression in horses. Eight clinically normal and regularly trained standardbred trotters were treadmill-exercised for 45 min at moderate intensity. Blood samples were collected prior to and immediately after exercise and at 4 and 24 h of recovery. Muscle biopsy samples from the middle gluteal muscle were taken before exercise and after 4 h of recovery. Acute exercise did not activate HSF1 or induce expression of HSP70, HSP90 or GRP75 in skeletal muscle. One bout of acute exercise increased protein oxidation, which was measured by protein carbonyls in plasma and muscle, but it did not effect 4-hydroxynonenal protein adducts, which are markers of lipid peroxidation. Furthermore, mild muscle damage was observed 4 h after exercise. Our results showed that horses are susceptible to oxidative stress. One bout of exercise at moderate intensity and duration did not induce HSP responses despite the increased protein oxidation and tissue inflammation in equine muscle.
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Publication Date: 2004-06-23 PubMed ID: 15221402DOI: 10.1007/s00421-004-1162-xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the effects of a single session of moderate intensity exercise on markers of oxidative stress and heat shock proteins in horses. It concludes that while exercise-induced oxidative stress was evident, it did not trigger the activation of heat shock proteins, despite causing a mild muscle damage.

Overview of the Research Study

  • The main goal of this study was to determine how a single session of moderate-intensity exercise impacts oxidative stress markers and the expression of heat shock proteins and glucose-regulated protein in horses.
  • The researchers utilized eight clinically normal and regularly trained standardbred trotters as test subjects. These trotters were made to exercise on a treadmill for 45 minutes at a moderate intensity.
  • For data collection, blood samples were collected at different intervals before and after the exercise, and muscle biopsy samples from the middle gluteal muscle were collected before the exercise and after 4 hours of recovery.

Findings of the Study

  • The study found that acute exercise did not result in the activation of the heat shock factor-1 (HSF1) or induce the expression of heat shock proteins (HSP70 and HSP90) or the glucose-regulated protein (GRP75) in skeletal muscle.
  • Instead, one bout of acute exercise resulted in protein oxidation, which was monitored using protein carbonyls in plasma and muscle.
  • Interestingly, the researchers noted that the exercise did not significantly affect markers of lipid peroxidation (4-hydroxynonenal protein adducts), contrary to their expectations.
  • A mild degree of muscle damage was also observed 4 hours post exercise, indicating tissue inflammation.

Conclusions of the Study

  • Based on these findings, the researchers concluded that horses are indeed susceptible to oxidative stress, especially during moderate-intensity exercise.
  • The absence of HSP responses after exercise, despite the evident oxidative stress and mild muscle damage, is an important observation which could shape future research in horse training and care.
  • Overall, this study provides insight into how a single bout of moderate-intensity exercise impacts horses at a cellular level.

Cite This Article

APA
Kinnunen S, Hyyppä S, Lappalainen J, Oksala N, Venojärvi M, Nakao C, Hänninen O, Sen CK, Atalay M. (2004). Exercise-induced oxidative stress and muscle stress protein responses in trotters. Eur J Appl Physiol, 93(4), 496-501. https://doi.org/10.1007/s00421-004-1162-x

Publication

European journal of applied physiology
ISSN: 1439-6319
NlmUniqueID: 100954790
Country: Germany
Language: English
Volume: 93
Issue: 4
Pages: 496-501

Researcher Affiliations

Kinnunen, Susanna
  • Department of Physiology, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland.
Hyyppä, Seppo
    Lappalainen, Jani
      Oksala, Niku
        Venojärvi, Mika
          Nakao, Chitose
            Hänninen, Osmo
              Sen, Chandan K
                Atalay, Mustafa

                  MeSH Terms

                  • Animals
                  • Female
                  • Heat-Shock Proteins / metabolism
                  • Horses / physiology
                  • Male
                  • Muscle, Skeletal / physiology
                  • Oxidative Stress / physiology
                  • Physical Conditioning, Animal / methods
                  • Physical Endurance / physiology
                  • Physical Exertion / physiology
                  • Running / physiology

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