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Home / Equine Research Bank / Environ Sci Pollut Res Int / Exogenous enzyme amendment accelerates maturity and changes ...
Environmental science and pollution research international2021; 28(17); 21610-21620; doi: 10.1007/s11356-020-11568-4

Exogenous enzyme amendment accelerates maturity and changes microflora succession in horse and wildlife animal manure co-composting.

Abstract: Composting has been a rational method to dispose of horse or wildlife animal manures, especially in the developed cities with horse clubs and wildlife parks. However, few studies have focused on the mechanism and improvement methods for composting the horse or wildlife animal manures. In this study, we investigated the effect of exogenous compound enzymes on thermophilic composting, which could potentially support the management of horse and wildlife animal manures. With the presence of exogenous enzymes, the duration of high temperature (> 60 °C) was significantly prolonged (p < 0.05), and the germination index was significantly improved (p < 0.05). More-efficient improvement of composting maturity was associated with the addition of that exogenous enzyme that might influence microflora succession and the interaction among microorganic communities, especially fungal, during the composting process. Furthermore, redundancy and canonical correspondence analyses indicated that the C/N ratio, temperature, and germination index were significant variations to influence bacterial communities (p < 0.05). The dominant Flavobacterium, Thermopolyspora, Thermomonospora, and Chaetomium and Saccobolus could play an essential role in carbohydrate and phytotoxin degradation, while Thermobispora and norank_f_Limnochordaceae could lead to temperature rising.
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Publication Date: 2021-01-07 PubMed ID: 33415619DOI: 10.1007/s11356-020-11568-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates how the use of external enzymes in composting improves the composting process and efficiency of horse and wildlife animal manures, and also influences the composition of microbial communities that are essential in the process.

Objectives and Approach

  • The study aimed at understanding the impact of exogenous (externally derived) enzymes on the thermophilic composting process. Thermophilic composting implies the process is performed at high temperatures, which can quicken decomposition and eliminate pathogens.
  • The research, while trying to optimize composting processes, especially in developed cities with horse clubs and wildlife parks, sought means of creating a more efficient composting process and understanding the mechanism through which it operates.

Key Findings

  • Exogenous enzymes prolonged the duration of high temperatures above 60 degrees Celsius, improving the composting efficiency. High temperatures are beneficial for composting as they accelerate decomposition by providing an ideal environment for thermophilic microorganisms that break down complex compounds into more manageable compounds.
  • These enzymes significantly improved the germination index, which is a measure of the compost’s readiness for use in agriculture. A higher germination index implies a greater fertility potential of the compost.
  • The introduction of exogenous enzymes influenced the succession and interaction of the microflora involved in the composting process, most notably fungal communities.
  • The study highlighted the critical role of specific bacterial communities, such as Flavobacterium, Thermopolyspora, Thermomonospora, and Chaetomium and Saccobolus in carbohydrate and phytotoxin degradation during composting. Other communities like Thermobispora and norank_f_Limnochordaceae were influential in raising the compost pile’s temperature.

Implications

  • The study’s findings suggest that the introduction of external enzymes could help manage and optimize waste management strategies for horse and wildlife animal manures by effectively fast-tracking the composting process and improving the end product’s quality.
  • Understanding the key bacterial communities’ role in the process could lead to even more targeted strategies that could improve composting efficacy, and minimize potential harm to the environment.

Cite This Article

APA
Du G, Feng W, Cai H, Ma Z, Liu X, Yuan C, Shi J, Zhang B. (2021). Exogenous enzyme amendment accelerates maturity and changes microflora succession in horse and wildlife animal manure co-composting. Environ Sci Pollut Res Int, 28(17), 21610-21620. https://doi.org/10.1007/s11356-020-11568-4

Publication

Environmental science and pollution research international
ISSN: 1614-7499
NlmUniqueID: 9441769
Country: Germany
Language: English
Volume: 28
Issue: 17
Pages: 21610-21620

Researcher Affiliations

Du, Guilin
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
  • School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Feng, Wenwen
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Cai, Hanbin
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Ma, Zhiguo
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Liu, Xiangcen
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
Yuan, Chenyang
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China.
  • University of Chinese Academy of Sciences, Beijing, 100049, China.
  • School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Shi, Jiping
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China. shijp@sari.ac.cn.
  • University of Chinese Academy of Sciences, Beijing, 100049, China. shijp@sari.ac.cn.
  • School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China. shijp@sari.ac.cn.
Zhang, Baoguo
  • Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, China. zhangbg@sari.ac.cn.
  • University of Chinese Academy of Sciences, Beijing, 100049, China. zhangbg@sari.ac.cn.

MeSH Terms

  • Animals
  • Animals, Wild
  • Cities
  • Composting
  • Horses
  • Manure
  • Soil

Grant Funding

  • 2017YFD0800205 / the National Key R&D Program of China

References

This article includes 37 references
  1. Antunes LP, Martins LF, Pereira RV, Thomas AM, Barbosa D, Lemos LN. Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics. Sci Rep 6:38915.
    doi: 10.1038/srep38915google scholar: lookup
  2. Ao Y, Yang C, Wang SHQ. Characteristics and nutrient function of intestinal bacterial communities in black soldier fly (Hermetia illucens L.) larvae in livestock manure conversion. Microb Biotechnol 11.
    doi: 10.1111/1751-7915.13595google scholar: lookup
  3. Arab G, McCartney D. Benefits to decomposition rates when using digestate as compost co-feedstock: part I - focus on physicochemical parameters. Waste Manag 68:74–84.
    doi: 10.1016/j.wasman.2017.07.018google scholar: lookup
  4. Awasthi MK, Pandey AK, Khan J, Bundela PS, Wong JW, Selvam A. Evaluation of thermophilic fungal consortium for organic municipal solid waste composting. Bioresour Technol 168:214–221.
    doi: 10.1016/j.biortech.2014.01.048google scholar: lookup
  5. Awasthi MK, Pandey AK, Bundela PS, Khan J. Co-composting of organic fraction of municipal solid waste mixed with different bulking waste: characterization of physicochemical parameters and microbial enzymatic dynamic. Bioresour Technol 182:200–207.
    doi: 10.1016/j.biortech.2015.01.104google scholar: lookup
  6. Bader GD, Hogue CW. An automated method for finding molecular complexes in large protein interaction networks. BMC Bioinformatics 4:2.
    doi: 10.1186/1471-2105-4-2google scholar: lookup
  7. Banerjee S, Baah-Acheamfour M, Carlyle CN, Bissett A, Richardson AE, Siddique T, Bork EW, Chang SX. Determinants of bacterial communities in Canadian agroforestry systems. Environ Microbiol 18:1805–1816.
    doi: 10.1111/1462-2920.12986google scholar: lookup
  8. Banerjee S, Kirkby CA, Schmutter D, Bissett A, Kirkegaard JA, Richardson AE. Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil. Soil Biol Biochem 97:188–198.
    doi: 10.1016/j.soilbio.2016.03.017google scholar: lookup
  9. Bernal MP, Alburquerque JA, Moral R. Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresour Technol 100:5444–5453.
    doi: 10.1016/j.biortech.2008.11.027google scholar: lookup
  10. Fang Y, Jia XB, Chen LJ, Lin CQ, Zhang H, Chen JC. Effect of thermotolerant bacterial inoculation on the microbial community during sludge composting. Can J Microbiol 65:750–761.
    doi: 10.1139/cjm-2019-0107google scholar: lookup
  11. Feng CL, Zeng GM, Huang DL, Hu S, Zhao MH, Lai C. Effect of ligninolytic enzymes on lignin degradation and carbon utilization during lignocellulosic waste composting. Process Biochem 46:1515–1520.
    doi: 10.1016/j.procbio.2011.01.038google scholar: lookup
  12. Hadin Å, Eriksson O, Hillman K. A review of potential critical factors in horse keeping for anaerobic digestion of horse manure. Renew Sust Energ Rev 65:432–442.
    doi: 10.1016/j.rser.2016.06.058google scholar: lookup
  13. Harindintwali JD, Zhou J, Yu X. Lignocellulosic crop residue composting by cellulolytic nitrogen-fixing bacteria: a novel tool for environmental sustainability. Sci Total Environ 715:136912.
    doi: 10.1016/j.scitotenv.2020.136912google scholar: lookup
  14. Jiang X, Deng L, Meng Q, Sun Y, Xu X. Fungal community succession under influence of biochar in cow manure composting. Environ Sci Pollut Res Int 27:9658–9668.
    doi: 10.1007/s11356-019-07529-1google scholar: lookup
  15. Krishnan Y, Bong CPC, Azman NF, Zakaria Z, Othman N’A, Abdullah N. Co-composting of palm empty fruit bunch and palm oil mill effluent: microbial diversity and potential mitigation of greenhouse gas emission. J Clean Prod 146:94–100.
    doi: 10.1016/j.jclepro.2016.08.118google scholar: lookup
  16. Langarica-Fuentes A, Zafar U, Heyworth A, Brown T, Fox G, Robson GD. Fungal succession in an in-vessel composting system characterized using 454 pyrosequencing. FEMS Microbiol Ecol 88:296–308.
    doi: 10.1111/1574-6941.12293google scholar: lookup
  17. Li X, Shi XS, Lu MY, Zhao YZ, Li XQ, Peng H, Guo RB. Succession of the bacterial community and functional characteristics during continuous thermophilic composting of dairy manure amended with recycled ceramsite. Bioresour Technol 294:9.
    doi: 10.1016/j.biortech.2018.11.075google scholar: lookup
  18. Liao HP, Lu X, Rensing C, Friman VP, Geisen S, Chen Z. Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge. Environ Sci Technol 52:266–276.
    doi: 10.1021/acs.est.7b04483google scholar: lookup
  19. Liggenstoffer AS, Youssef NH, Couger MB, Elshahed MS. Phylogenetic diversity and community structure of anaerobic gut fungi (phylum Neocallimastigomycota) in ruminant and non-ruminant herbivores. ISME J 4:1225–1235.
    doi: 10.1038/ismej.2010.49google scholar: lookup
  20. Ma L, Zhao B, Guo Z, Wang D, Li D, Xu J. Divergent responses of bacterial activity, structure, and co-occurrence patterns to long-term unbalanced fertilization without nitrogen, phosphorus, or potassium in a cultivated vertisol. Environ Sci Pollut Res Int 26:12741–12754.
    doi: 10.1007/s11356-019-04839-2google scholar: lookup
  21. Mao HL, Wang K, Wang Z, Peng J, Ren NQ. Metabolic function, trophic mode, organics degradation ability and influence factor of bacterial and fungal communities in chicken manure composting. Bioresour Technol 302:9.
    doi: 10.1016/j.biortech.2020.122883google scholar: lookup
  22. Martins LF, Antunes LP, Pascon RC. Metagenomic analysis of a tropical composting operation at the Sao Paulo zoo park reveals diversity of biomass degradation functions and organisms. PLoS One 8:e61928.
    doi: 10.1371/journal.pone.0061928google scholar: lookup
  23. Melo RFR, Miller AN, Santiago ALCMA, Maia LC. The genera Ascobolus and Saccobolus (Ascobolaceae, Pezizales) in Brazil. Mycosphere 5:790–804.
    doi: 10.5943/mycosphere/5/6/9google scholar: lookup
  24. Meng X, Yan J, Zuo B, Wang Y, Yuan X, Cui Z. Full-scale of composting process of biogas residues from corn stover anaerobic digestion: physical-chemical, biology parameters and maturity indexes during whole process. Bioresour Technol 302:122742.
    doi: 10.1016/j.biortech.2020.122742google scholar: lookup
  25. Mungai PG, Njogu JG, Hyde KD. Coprophilous ascomycetes in Kenya: Saccobolus species from wildlife dung. Mycosphere 3:111–129.
    doi: 10.5943/mycosphere/3/2/2google scholar: lookup
  26. Peng J, Wang K, Yin X, Yin X, Du M, Gao Y. Trophic mode and organics metabolic characteristic of fungal community in swine manure composting. Front Environ Sci Eng 13.
    doi: 10.1007/s11783-019-1177-5google scholar: lookup
  27. Perez-Godinez EA, Lagunes-Zarate J, Corona-Hernandez J, Barajas-Aceves M. Growth and reproductive potential of Eisenia foetida (Sav) on various zoo animal dungs after two methods of pre-composting followed by vermicomposting. Waste Manag 64:67–78.
    doi: 10.1016/j.wasman.2017.03.036google scholar: lookup
  28. Pertile G, Panek J, Oszust K, Siczek A, Frac M. Intraspecific functional and genetic diversity of Petriella setifera. Peerj 6:e4420.
    doi: 10.7717/peerj.4420google scholar: lookup
  29. Riikka Keskinen MS, Nikama J, Susanna Särkijärvi MMJU-K. Recycling nutrients from horse manure: effects of bedding type and its compostability. Agric Food Sci 26:68–79.
  30. Wang X, Cui H, Shi J, Zhao X, Zhao Y, Wei Z. Relationship between bacterial diversity and environmental parameters during composting of different raw materials. Bioresour Technol 198:395–402.
    doi: 10.1016/j.biortech.2015.09.041google scholar: lookup
  31. Wang C, Dong D, Wang HS, Muller K, Qin Y, Wang HL, Wu WX. Metagenomic analysis of microbial consortia enriched from compost: new insights into the role of Actinobacteria in lignocellulose decomposition. Biotechnol Biofuels 9:22.
    doi: 10.1186/s13068-016-0440-2google scholar: lookup
  32. Xi BD, He XS, Dang QL, Yang TX, Li MX, Wang XW, Li D, Tang J. Effect of multi-stage inoculation on the bacterial and fungal community structure during organic municipal solid wastes composting. Bioresour Technol 196:399–405.
    doi: 10.1016/j.biortech.2015.07.069google scholar: lookup
  33. Yin Y, Gu J, Wang X, Zhang Y, Zheng W, Chen R, Wang X. Effects of rhamnolipid and Tween-80 on cellulase activities and metabolic functions of the bacterial community during chicken manure composting. Bioresour Technol 288:121507.
    doi: 10.1016/j.biortech.2019.121507google scholar: lookup
  34. Yu Z, Zeng GM, Chen YN, Zhang JC, Yu Y, Li H. Effects of inoculation with Phanerochaete chrysosporium on remediation of pentachlorophenol-contaminated soil waste by composting. Process Biochem 46:1285–1291.
    doi: 10.1016/j.procbio.2011.02.018google scholar: lookup
  35. Zhang Q, Liu J, Guo H, Li E, Yan Y. Characteristics and optimization of dairy manure composting for reuse as a dairy mattress in areas with large temperature differences. J Clean Prod 232:1053–1061.
    doi: 10.1016/j.jclepro.2019.05.397google scholar: lookup
  36. Zhao X, Wei Y, Fan Y, Zhang F, Tan W, He X, Xi B. Roles of bacterial community in the transformation of dissolved organic matter for the stability and safety of material during sludge composting. Bioresour Technol 267:378–385.
    doi: 10.1016/j.biortech.2018.07.060google scholar: lookup
  37. Zhou G, Qiu X, Chen L, Zhang C, Ma D, Zhang J. Succession of organics metabolic function of bacterial community in response to addition of earthworm casts and zeolite in maize straw composting. Bioresour Technol 280:229–238.
    doi: 10.1016/j.biortech.2019.02.015google scholar: lookup

Citations

This article has been cited 5 times.
  1. Liu C, Han D, Yang H, Liu Z, Gao C, Liu Y. Effects of peach branch organic fertilizer on the soil microbial community in peach orachards. Front Microbiol 2023;14:1223420.
    doi: 10.3389/fmicb.2023.1223420pubmed: 37485500google scholar: lookup
  2. Duan H, Fu C, Du G, Xie S, Liu M, Zhang B, Shi J, Sun J. Dynamic Microstructure Assembly Driven by Lysinibacillus sp. LF-N1 and Penicillium oxalicum DH-1 Inoculants Corresponds to Composting Performance. Microorganisms 2022 Mar 25;10(4).
    doi: 10.3390/microorganisms10040709pubmed: 35456760google scholar: lookup
  3. Chen X, Wu C, Li X, Wang C, Li Q, Zhou P, Wei D, Shi J, Zhao Z. Effect of Geobacillus toebii GT-02 addition on composition transformations and microbial community during thermophilic fermentation of bean dregs. Sci Rep 2021 Oct 7;11(1):19949.
    doi: 10.1038/s41598-021-99413-7pubmed: 34620903google scholar: lookup
  4. Du G, Zhang G, Shi J, Zhang J, Ma Z, Liu X, Yuan C, Li X, Zhang B. Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw. Microorganisms 2021 May 20;9(5).
    doi: 10.3390/microorganisms9051099pubmed: 34065243google scholar: lookup
  5. Du G, Shi J, Zhang J, Ma Z, Liu X, Yuan C, Zhang B, Zhang Z, Harrison MD. Exogenous Probiotics Improve Fermentation Quality, Microflora Phenotypes, and Trophic Modes of Fermented Vegetable Waste for Animal Feed. Microorganisms 2021 Mar 19;9(3).
    doi: 10.3390/microorganisms9030644pubmed: 33808890google scholar: lookup
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