English
French
Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression in three-dimensional culture which can be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signaling.
Abstract: We investigated how Interleukin 1 beta (IL-1β) impacts equine tenocyte function and global gene expression in vitro and determined if these effects could be rescued by pharmacologically inhibiting nuclear factor-κB (NF-B) or interleukin 1 signalling. Equine superficial digital flexor tenocytes were cultured in three-dimensional (3D) collagen gels and stimulated with IL-1β for two-weeks, with gel contraction and interleukin 6 (IL6) measured throughout and transcriptomic analysis performed at day 14. The impact of three NF-B inhibitors on gel contraction and IL6 secretion were measured in 3D culture, with NF-B-P65 nuclear translocation by immunofluorescence and gene expression by qPCR measured in two-dimensional (2D) monolayer culture. In addition, daily 3D gel contraction and transcriptomic analysis was performed on interleukin 1 receptor antagonist-treated 3D gels at day 14. IL-1β increased NF-B-P65 nuclear translocation in 2D culture and IL6 secretion in 3D culture, but reduced daily tenocyte 3D gel contraction and impacted > 2500 genes at day 14, with enrichment for NF-B signaling. Administering direct pharmacological inhibitors of NF-B did reduce NF-B-P65 nuclear translocation, but had no effect on 3D gel contraction or IL6 secretion in the presence of IL-1β. However, IL1Ra restored 3D gel contraction and partially rescued global gene expression. Tenocyte 3D gel contraction and gene expression is adversely impacted by IL-1β which can only be rescued by blockade of interleukin 1 receptor, but not NF-B, signalling.
© 2023. The Author(s).
Publication Date: 2023-06-14 PubMed ID: 37314623PubMed Central: 3284560DOI: 10.1007/s11010-023-04779-zGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates the effect of interleukin 1 beta on the functionality and gene expression of equine tenocytes, and evaluates the role of pharmacological inhibitors in preventing these effects.
Experiment Overview
- The researchers conducted in vitro studies on equine superficial digital flexor tenocytes, a type of cell crucial to the health and functioning of horse tendons. These tenocytes were cultured in three-dimensional (3D) collagen gels and stimulated with interleukin-1 beta (IL-1β), an inflammatory protein, over a period of two weeks.
- They monitored the gel contraction and interleukin 6 (IL6) secretion levels throughout the experiment and performed transcriptomic analysis—a study of RNA molecules—at day 14 to understand the impact of IL-1β on gene expression.
- In parallel, the researchers assessed the impact of multiple nuclear factor-κB (NF-B) inhibitors on the gel contraction, IL6 secretion, and gene expression in both 3D culture and two-dimensional monolayer culture.
- Finally, the effects of interleukin 1 receptor antagonist (IL1Ra) treatment on the 3D gels were also investigated through contraction and transcriptomic analysis at day 14.
Key Findings
- IL-1β was responsible for certain alterations in tenocyte behavior, notably a decrease in 3D gel contraction and a spike in IL6 secretion, with over 2500 genes impacted by the end of the two-week period. It also increased NF-B-P65 (a protein that plays a crucial role in inflammation) nuclear translocation in 2D culture.
- The application of direct pharmacological inhibitors of NF-B successfully reduced NF-B-P65 nuclear translocation but had no impact on the IL-1β-induced alterations in 3D gel contraction or IL6 secretion.
- Contrarily, IL1Ra treatment successfully restored the 3D gel contraction and partially mitigated the impact on global gene expression caused by IL-1β, demonstrating its potential as a therapeutic agent in mitigating IL-1β’s negative effects on tenocyte function.
Conclusion
- The study’s findings suggest that the damaging influence of IL-1β on tenocytes, which includes negatively impacting gel contraction and gene expression, can only be effectively mitigated by blocking the interleukin 1 receptor rather than by inhibiting NF-B signaling.
Cite This Article
APA
Beaumont RE, Smith EJ, Zhou L, Marr N, Thorpe CT, Guest DJ.
(2023).
Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression in three-dimensional culture which can be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signaling.
Mol Cell Biochem.
https://doi.org/10.1007/s11010-023-04779-z Publication
Researcher Affiliations
- Clinical Sciences and Service, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, AL9 7TA, Herts, UK. robeaumont@rvc.ac.uk.
- Clinical Sciences and Service, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, AL9 7TA, Herts, UK.
- Clinical Sciences and Service, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, AL9 7TA, Herts, UK.
- Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.
- Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.
- Clinical Sciences and Service, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, AL9 7TA, Herts, UK.
Grant Funding
- 21216/22607 / Versus Arthritis
- 21216/22607 / Versus Arthritis
References
This article includes 89 references
- Benjamin M, Kaiser E, Milz S (2008) Structure-function relationships in tendons: a review. J Anatomy 212:211u2013218. https://doi.org/10.1111/j.1469-7580.2008.00864.x
- Kannus P (1997) Tendonsu2014a source of major concern in competative and recreational athletes. ScandaJ. Med Sci Sports 7:53u201354
- Docheva D, Mu00fcller SA, Majewski M, Evans CH. Biologics for tendon repair.. Adv Drug Deliv Rev 2015 Apr;84:222-39.
- Nourissat G, Berenbaum F, Duprez D. Tendon injury: from biology to tendon repair.. Nat Rev Rheumatol 2015 Apr;11(4):223-33.
- Williams RB, Harkins LS, Hammond CJ, Wood JL. Racehorse injuries, clinical problems and fatalities recorded on British racecourses from flat racing and National Hunt racing during 1996, 1997 and 1998.. Equine Vet J 2001 Sep;33(5):478-86.
- Riley G. The pathogenesis of tendinopathy. A molecular perspective.. Rheumatology (Oxford) 2004 Feb;43(2):131-42.
- Dakin SG, Werling D, Hibbert A, Abayasekara DR, Young NJ, Smith RK, Dudhia J. Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair.. PLoS One 2012;7(2):e32333.
- Dyson SJ. Medical management of superficial digital flexor tendonitis: a comparative study in 219 horses (1992-2000).. Equine Vet J 2004 Jul;36(5):415-9.
- Perkins NR, Reid SWJ, Morris RS (2005) Risk factors for injury to the superficial digital flexor tendon and suspensory apparatus in Thoroughbred racehorses in New Zealand. NZ Vet J 53:184u2013192. https://doi.org/10.1080/00480169.2005.36503
- Schepsis AA, Jones H, Haas AL. Achilles tendon disorders in athletes.. Am J Sports Med 2002 Mar-Apr;30(2):287-305.
- Patterson-Kane JC, Rich T. Achilles tendon injuries in elite athletes: lessons in pathophysiology from their equine counterparts.. ILAR J 2014;55(1):86-99.
- Oreff GL, Fenu M, Vogl C, Ribitsch I, Jenner F. Species variations inu00a0tenocytes' response to inflammation require careful selection of animal models for tendon research.. Sci Rep 2021 Jun 14;11(1):12451.
- Patterson-Kane JC, Becker DL, Rich T. The pathogenesis of tendon microdamage in athletes: the horse as a natural model for basic cellular research.. J Comp Pathol 2012 Aug-Oct;147(2-3):227-47.
- Alfredson H, Lorentzon R. Chronic tendon pain: no signs of chemical inflammation but high concentrations of the neurotransmitter glutamate. Implications for treatment?. Curr Drug Targets 2002 Feb;3(1):43-54.
- Dakin SG, Dudhia J, Smith RK. Resolving an inflammatory concept: the importance of inflammation and resolution in tendinopathy.. Vet Immunol Immunopathol 2014 Apr 15;158(3-4):121-7.
- Jomaa G, Kwan CK, Fu SC, Ling SK, Chan KM, Yung PS, Rolf C. A systematic review of inflammatory cells and markers in human tendinopathy.. BMC Musculoskelet Disord 2020 Feb 6;21(1):78.
- Rees JD, Stride M, Scott A. Tendons--time to revisit inflammation.. Br J Sports Med 2014 Nov;48(21):1553-7.
- Dakin SG, Martinez FO, Yapp C, Wells G, Oppermann U, Dean BJ, Smith RD, Wheway K, Watkins B, Roche L, Carr AJ. Inflammation activation and resolution in human tendon disease.. Sci Transl Med 2015 Oct 28;7(311):311ra173.
- Best KT, Nichols AEC, Knapp E, Hammert WC, Ketonis C, Jonason JH, Awad HA, Loiselle AE. NF-u03baB activation persists into the remodeling phase of tendon healing and promotes myofibroblast survival.. Sci Signal 2020 Nov 17;13(658).
- Millar NL, Murrell GA, McInnes IB. Inflammatory mechanisms in tendinopathy - towards translation.. Nat Rev Rheumatol 2017 Jan 25;13(2):110-122.
- Arvind V, Huang AH (2021) Reparative and maladaptive inflammation in tendon healing. Front Bioeng Biotechnol 19:719047. https://doi.org/10.3389/fbioe.2021.719047
- Morita W, Dakin SG, Snelling SJB, Carr AJ. Cytokines in tendon disease: A Systematic Review.. Bone Joint Res 2017 Dec;6(12):656-664.
- Abraham AC, Shah SA, Golman M, Song L, Li X, Kurtaliaj I, Akbar M, Millar NL, Abu-Amer Y, Galatz LM, Thomopoulos S. Targeting the NF-u03baB signaling pathway in chronic tendon disease.. Sci Transl Med 2019 Feb 27;11(481).
- Hosaka Y, Kirisawa R, Yamamoto E, Ueda H, Iwai H, Takehana K. Localization of cytokines in tendinocytes of the superficial digital flexor tendon in the horse.. J Vet Med Sci 2002 Oct;64(10):945-7.
- Koch DW, Berglund AK, Messenger KM, Gilbertie JM, Ellis IM, Schnabel LV. Interleukin-1u03b2 in tendon injury enhances reparative gene and protein expression in mesenchymal stem cells.. Front Vet Sci 2022;9:963759.
- Smith E, Beaumont R, McClellan A, Sze C, Palomino Lago E, Hazelgrove L, Dudhia J, Smith R, Guest D (in press) Tumour necrosis factor alpha, interleukin 1 beta and interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell-derived factors. Cell and Tissue Res 391(3): 523u2013544. https://doi.org/10.1007/s00441-022-03726-6
- McCellan A, Evans R, Sze C, Kan S, Paterson Y, Guest D (2019) A novel mechanim for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta. Sci Rep 9:275. https://doi.org/10.1038/s41598-019-39370-4
- Minkwitz S, Schmock A, Kurtoglu A, Tsitsilonis S, Manegold S, Wildemann B, Klatte-Schulz F. Time-Dependent Alterations of MMPs, TIMPs and Tendon Structure in Human Achilles Tendons after Acute Rupture.. Int J Mol Sci 2017 Oct 20;18(10).
- Gehwolf R, Schwemberger B, Jessen M, Korntner S, Wagner A, Lehner C, Weissenbacher N, Tempfer H, Traweger A. Global Responses of Il-1u03b2-Primed 3D Tendon Constructs to Treatment with Pulsed Electromagnetic Fields.. Cells 2019 Apr 30;8(5).
- Stolk M, Klatte-Schulz F, Schmock A, Minkwitz S, Wildemann B, Seifert M. New insights into tenocyte-immune cell interplay in an in vitro model of inflammation.. Sci Rep 2017 Aug 29;7(1):9801.
- Tsuzaki M, Guyton G, Garrett W, Archambault JM, Herzog W, Almekinders L, Bynum D, Yang X, Banes AJ (2003) IL-1 beta induces COX2, MMP-1, -3 and -13, ADAMTS-4, IL-1 beta and IL-6 in human tendon cells. J Orthopedic Res 21:256u2013264. https://doi.org/10.1016/S0736-0266(02)00141-9
- Garcia-Melchor E, Cafaro G, MacDonald L, Crowe LAN, Sood S, McLean M, Fazzi UG, McInnes I, Akbar M, Millar NL (2021) Novel self-amplificatory loop between T cells and tenocytes as a driver of chronicity in tendon disease. Ann Rhematic Dis 80:1075u20131085. https://doi.org/10.1136/annrheumdis-2020-219335
- Ackerman JE, Best KT, Muscat SN, Loiselle AE. Metabolic Regulation of Tendon Inflammation and Healing Following Injury.. Curr Rheumatol Rep 2021 Feb 10;23(3):15.
- Lustgarten M, Redding WR, Labens R, Davis W, Daniel TM, Griffith E, Seiler GS. ELASTOGRAPHIC EVALUATION OF NATURALLY OCCURING TENDON AND LIGAMENT INJURIES OF THE EQUINE DISTAL LIMB.. Vet Radiol Ultrasound 2015 Nov-Dec;56(6):670-9.
- Connizzo BK, Bhatt PR, Liechty KW, Soslowsky LJ. Diabetes alters mechanical properties and collagen fiber re-alignment in multiple mouse tendons.. Ann Biomed Eng 2014 Sep;42(9):1880-8.
- Bosch G, Moleman M, Barneveld A, van Weeren PR, van Schie HT. The effect of platelet-rich plasma on the neovascularization of surgically created equine superficial digital flexor tendon lesions.. Scand J Med Sci Sports 2011 Aug;21(4):554-61.
- Tang JB, Wu YF, Cao Y, Chen CH, Zhou YL, Avanessian B, Shimada M, Wang XT, Liu PY (2016) Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons. Sci Rep 6:1u201312. https://doi.org/10.1038/srep20643
- Kim SE, Kim JG, Park K. Biomaterials for the Treatment of Tendon Injury.. Tissue Eng Regen Med 2019 Oct;16(5):467-477.
- Smith RK. Mesenchymal stem cell therapy for equine tendinopathy.. Disabil Rehabil 2008;30(20-22):1752-8.
- Geburek F, Gaus M, van Schie HT, Rohn K, Stadler PM. Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial.. BMC Vet Res 2016 Sep 7;12(1):191.
- Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model.. BMC Musculoskelet Disord 2018 Jul 18;19(1):230.
- Brossi PM, Moreira JJ, Machado TS, Baccarin RY. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions.. BMC Vet Res 2015 Apr 22;11:98.
- Dinarello CA, Simon A, van der Meer JW. Treating inflammation by blocking interleukin-1 in a broad spectrum of diseases.. Nat Rev Drug Discov 2012 Aug;11(8):633-52.
- Goodrich LR, Grieger JC, Phillips JN, Khan N, Gray SJ, McIlwraith CW, Samulski RJ. scAAVIL-1ra dosing trial in a large animal model and validation of long-term expression with repeat administration for osteoarthritis therapy.. Gene Ther 2015 Jul;22(7):536-45.
- Mehta S, Akhtar S, Porter RM, u00d6nnerfjord P, Bajpayee AG. Interleukin-1 receptor antagonist (IL-1Ra) is more effective in suppressing cytokine-induced catabolism in cartilage-synovium co-culture than in cartilage monoculture.. Arthritis Res Ther 2019 Nov 13;21(1):238.
- Eskildsen SM, Berkoff DJ, Kallianos SA, Weinhold PS. The use of an IL1-receptor antagonist to reverse the changes associated with established tendinopathy in a rat model.. Scand J Med Sci Sports 2019 Jan;29(1):82-88.
- Lin FS, Lin CC, Chien CS, Luo SF, Yang CM. Involvement of p42/p44 MAPK, JNK, and NF-kappaB in IL-1beta-induced ICAM-1 expression in human pulmonary epithelial cells.. J Cell Physiol 2005 Feb;202(2):464-73.
- Li J, Stoppato M, Schiele NR, Graybeal KL, Nguyen PK, Kuo CK. Embryonic and postnatal tendon cells respond differently to interleukin-1u03b2.. Ann N Y Acad Sci 2019 Apr;1442(1):118-127.
- Morita W, Snelling SJB, Wheway K, Watkins B, Appleton L, Carr AJ, Dakin SG. ERK1/2 drives IL-1u03b2-induced expression of TGF-u03b21 and BMP-2 in torn tendons.. Sci Rep 2019 Dec 12;9(1):19005.
- Liu T, Zhang L, Joo D, Sun SC. NF-u03baB signaling in inflammation.. Signal Transduct Target Ther 2017;2:17023-.
- Chen S, Jiang S, Zheng W, Tu B, Liu S, Ruan H, Fan C. RelA/p65 inhibition prevents tendon adhesion by modulating inflammation, cell proliferation, and apoptosis.. Cell Death Dis 2017 Mar 30;8(3):e2710.
- Winkler A, Sun W, De S, Jiao A, Sharif MN, Symanowicz PT, Athale S, Shin JH, Wang J, Jacobson BA, Ramsey SJ, Dower K, Andreyeva T, Liu H, Hegen M, Homer BL, Brodfuehrer J, Tilley M, Gilbert SA, Danto SI, Beebe JJ, Barnes BJ, Pascual V, Lin LL, Kilty I, Fleming M, Rao VR. The Interleukin-1 Receptor-Associated Kinase 4 Inhibitor PF-06650833 Blocks Inflammation in Preclinical Models of Rheumatic Disease and in Humans Enrolled in a Randomized Clinical Trial.. Arthritis Rheumatol 2021 Dec;73(12):2206-2218.
- Paterson YZ, Cribbs A, Espenel M, Smith EJ, Henson FMD, Guest DJ. Genome-wide transcriptome analysis reveals equine embryonic stem cell-derived tenocytes resemble fetal, not adult tenocytes.. Stem Cell Res Ther 2020 May 19;11(1):184.
- Cummins KA, Bitterman PB, Tschumperlin DJ, Wood DK. A scalable 3D tissue culture pipeline to enable functional therapeutic screening for pulmonary fibrosis.. APL Bioeng 2021 Dec;5(4):046102.
- Imamura Y, Mukohara T, Shimono Y, Funakoshi Y, Chayahara N, Toyoda M, Kiyota N, Takao S, Kono S, Nakatsura T, Minami H. Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer.. Oncol Rep 2015 Apr;33(4):1837-43.
- Langhans SA (2018) Three-dimensional in vitro cell culture models in drug discovery and drug repositioning. Front Pharmacol 23:6. https://doi.org/10.3389/fphar.2018.00006
- Patro R, Duggal G, Love MI, Irizarry RA, Kingsford C. Salmon provides fast and bias-aware quantification of transcript expression.. Nat Methods 2017 Apr;14(4):417-419.
- Soneson C, Love MI, Robinson MD. Differential analyses for RNA-seq: transcript-level estimates improve gene-level inferences.. F1000Res 2015;4:1521.
- Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.. Genome Biol 2014;15(12):550.
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.. Methods 2001 Dec;25(4):402-8.
- Marr N, Hopkinson M, Hibbert AP, Pitsillides AA, Thopre CT (2020) Bimodal whole-mount imaging of tendon using confocal microscopy and x-ray micro-computed tomography. Biol Procedures Online 22:13. https://doi.org/10.1186/s12575-020-00126-4
- Marr N, Meeson R, Kelly EF, Fang Y, Peffers MJ, Pitsillides AA, Dudhia J, Thorpe CT. CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-u03b14.. Int J Mol Sci 2021 Sep 8;22(18).
- Danto SI, Shojaee N, Singh RSP, Li C, Gilbert SA, Manukyan Z, Kilty I. Safety, tolerability, pharmacokinetics, and pharmacodynamics of PF-06650833, a selective interleukin-1 receptor-associated kinase 4 (IRAK4) inhibitor, in single and multiple ascending dose randomized phase 1 studies in healthy subjects.. Arthritis Res Ther 2019 Dec 5;21(1):269.
- Eislmayr K, Bestehorn A, Morelli L, Borroni M, Vande Walle L, Lamkanfi M, Kovarik P. Nonredundancy of IL-1u03b1 and IL-1u03b2 is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.. Sci Adv 2022 Mar 4;8(9):eabj7293.
- Francisco-Cruz A, Aguilar-Santelises M, Ramos-Espinosa O, Mata-Espinosa D, Marquina-Castillo B, Barrios-Payan J, Hernandez-Pando R. Granulocyte-macrophage colony-stimulating factor: not just another haematopoietic growth factor.. Med Oncol 2014 Jan;31(1):774.
- Dakin SG, Coles M, Sherlock JP, Powrie F, Carr AJ, Buckley CD. Pathogenic stromal cells as therapeutic targets in joint inflammation.. Nat Rev Rheumatol 2018 Dec;14(12):714-726.
- Kendal AR, Layton T, Al-Mossawi H, Appleton L, Dakin S, Brown R, Loizou C, Rogers M, Sharp R, Carr A. Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon.. Sci Rep 2020 Sep 3;10(1):13939.
- Chen S, Deng G, Li K, Zheng H, Wang G, Yu B, Zhang K (2018) Interleukin-6 promotes proliferation but inhibits tenoenic differentiation via the janus kinase/signal transducers and activators of transcription 3 (JAK/STAT3) pathway in tendon-derived stem cells. Med Sci Monit 16:1567u20131573. https://doi.org/10.12659/MSM.908802
- Fernando MR, Reyes JL, Iannuzzi J, Leung G, McKay DM. The pro-inflammatory cytokine, interleukin-6, enhances the polarization of alternatively activated macrophages.. PLoS One 2014;9(4):e94188.
- Ackerman PW, Domeij-Arverud E, Leclerc P, Amoudrouz P, Nader GA (2013) Anti-inflammatory cytokine profile in early human tendon repair. Knee Surg Sports Traumatol Arthrosc 21:1801u20131806. https://doi.org/10.1007/s00167-012-2197-x
- Mu00fcller SA, Todorov A, Heisterbach PE, Martin I, Majewski M. Tendon healing: an overview of physiology, biology, and pathology of tendon healing and systematic review of state of the art in tendon bioengineering.. Knee Surg Sports Traumatol Arthrosc 2015 Jul;23(7):2097-105.
- Duque GA, Descoteaux A (2014) Macrophage cytokines: involvement in immunity and infectious disease. Front Immunol 5:1u201312. https://doi.org/10.3389/fimmu.2014.00491
- Blomgran P, Hammerman M, Aspenberg P. Systemic corticosteroids improve tendon healing when given after the early inflammatory phase.. Sci Rep 2017 Sep 29;7(1):12468.
- Golman M, Li X, Skouteris D, Abraham AA, Song L, Abu-Amer Y, Thomopoulos S. Enhanced Tendon-to-Bone Healing via IKKu03b2 Inhibition in a Rat Rotator Cuff Model.. Am J Sports Med 2021 Mar;49(3):780-789.
- Greten FR, Arkan MC, Bollrath J, Hsu LC, Goode J, Miething C, Gu00f6ktuna SI, Neuenhahn M, Fierer J, Paxian S, Van Rooijen N, Xu Y, O'Cain T, Jaffee BB, Busch DH, Duyster J, Schmid RM, Eckmann L, Karin M. NF-kappaB is a negative regulator of IL-1beta secretion as revealed by genetic and pharmacological inhibition of IKKbeta.. Cell 2007 Sep 7;130(5):918-31.
- De Nardo D, Balka KR, Cardona Gloria Y, Rao VR, Latz E, Masters SL. Interleukin-1 receptor-associated kinase 4 (IRAK4) plays a dual role in myddosome formation and Toll-like receptor signaling.. J Biol Chem 2018 Sep 28;293(39):15195-15207.
- Pauls E, Nanda SK, Smith H, Toth R, Arthur JSC, Cohen P. Two phases of inflammatory mediator production defined by the study of IRAK2 and IRAK1 knock-in mice.. J Immunol 2013 Sep 1;191(5):2717-30.
- Borza R, Salgado-Polo F, Moolenaar WH, Perrakis A. Structure and function of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family: Tidying up diversity.. J Biol Chem 2022 Feb;298(2):101526.
- Wu JM, Xu Y, Skill NJ, Sheng H, Zhao Z, Yu M, Saxena R, Maluccio MA (2010) Autotaxin expression and its connection with the TNF-alpha-NF-kappaB axis in human hepatocellular carcinoma. Mol Cancer 31:71. https://doi.org/10.1186/1476-4598-9-71
- Cu00f3rdova-Casanova A, Cruz-Soca M, Chun J, Casar JC, Brandan E. Activation of the ATX/LPA/LPARs axis induces a fibrotic response in skeletal muscle.. Matrix Biol 2022 May;109:121-139.
- Kaffe E, Katsifa A, Xylourgidis N, Ninou I, Zannikou M, Harokopos V, Foka P, Dimitriadis A, Evangelou K, Moulas AN, Georgopoulou U, Gorgoulis VG, Dalekos GN, Aidinis V. Hepatocyte autotaxin expression promotes liver fibrosis and cancer.. Hepatology 2017 Apr;65(4):1369-1383.
- Nikitopoulou I, Katsifa A, Kanellopoulou P, Jahaj E, Vassiliou AG, Mastora Z, Dimopoulou I, Orfanos SE, Aidinis V, Kotanidou A. Autotaxin Has a Negative Role in Systemic Inflammation.. Int J Mol Sci 2022 Jul 18;23(14).
- Lee Y, Sooranna SR, Terzidou V, Christian M, Brosens J, Huhtinen K, Poutanen M, Barton G, Johnson MR, Bennett PR. Interactions between inflammatory signals and the progesterone receptor in regulating gene expression in pregnant human uterine myocytes.. J Cell Mol Med 2012 Oct;16(10):2487-503.
- Latini FR, Hemerly JP, Oler G, Riggins GJ, Cerutti JM. Re-expression of ABI3-binding protein suppresses thyroid tumor growth by promoting senescence and inhibiting invasion.. Endocr Relat Cancer 2008 Sep;15(3):787-99.
- Delfu00edn DA, DeAguero JL, McKown EN. The Extracellular Matrix Protein ABI3BP in Cardiovascular Health and Disease.. Front Cardiovasc Med 2019;6:23.
- Igarashi N, Honjo M, Yamagishi R, Kurano M, Yatomi Y, Igarashi K, Kaburaki T, Aihara M. Crosstalk between transforming growth factor u03b2-2 and Autotaxin in trabecular meshwork and different subtypes of glaucoma.. J Biomed Sci 2021 Jun 17;28(1):47.
- Habanjar O, Diab-Assaf M, Caldefie-Chezet F, Delort L. 3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages.. Int J Mol Sci 2021 Nov 11;22(22).
- Hayn A, Fischer T, Mierke CT. Inhomogeneities in 3D Collagen Matrices Impact Matrix Mechanics and Cancer Cell Migration.. Front Cell Dev Biol 2020;8:593879.
- Yao L, Bestwick CS, Bestwick LA, Maffulli N, Aspden RM. Phenotypic drift in human tenocyte culture.. Tissue Eng 2006 Jul;12(7):1843-9.
Citations
This article has been cited 0 times.Secure Payments
