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Home / Equine Research Bank / Viruses / Experimental Infection of Horses with Influenza D Virus.
Viruses2022; 14(4); doi: 10.3390/v14040661

Experimental Infection of Horses with Influenza D Virus.

Abstract: Antibodies to influenza D virus (IDV) have been detected in horses, but no evidence of disease in the field has been reported. To determine whether IDV is infectious, immunogenic, and pathogenic in horses, four 2-year-old horses seronegative for both influenza A (H3N8) and D viruses were intranasally inoculated with 6.25 × 107 TCID50/animal of D/bovine/California/0363/2019 (D/CA2019) virus, using a portable equine nebulizer system. Horses were observed daily for clinical signs including rectal temperature, nasal discharge, coughing, lung sounds, tachycardia, and tachypnea. No horses exhibited clinical signs of disease. Nasopharyngeal swabs collected from 1-8 days post-infection demonstrated virus shedding by qRT-PCR. The horses showed evidence of seroconversion as early as 13 days post-infection (dpi) and the geometric mean of the antibody titers (GMT) of all four horses ranged from 16.82-160 as demonstrated by the microneutralization assay. Further, deep RNA sequencing of the virus isolated in embryonated chicken eggs revealed no adaptive mutations indicating that IDV can replicate in horses, suggesting the possibility of interspecies transmission of IDV with bovine reservoir into equids in nature.
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Publication Date: 2022-03-23 PubMed ID: 35458390PubMed Central: PMC9029652DOI: 10.3390/v14040661Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored if the influenza D virus (IDV) can infect, trigger an immune response, and cause diseases in horses. Using four 2-year-old horses, the experiment found that while the horses didn’t exhibit any disease symptoms, they shed the virus and developed antibodies against it. Sequencing of the isolated virus showed no mutations, indicating that IDV can replicate in horses, potentially leading to interspecies transmission between cattle and horses.

Research Methodology

  • Four 2-year-old horses that had not been exposed to influenza A or D were selected for the study.
  • Each horse was inoculated with the D/bovine/California/0363/2019 (D/CA2019) strain of IDV using a portable equine nebulizer system.
  • The horses’ health was monitored daily for signs of illness such as fever, nasal discharge, cough, abnormal lung sounds, and rapid heart rate or breathing.
  • Nasopharyngeal swabs were taken from the horses from day 1 to day 8 post-infection to check for virus shedding.

Results

  • The horses did not exhibit any clinical signs of illness.
  • The nasopharyngeal swabs demonstrated that the horses were shedding the virus, as detected by the qRT PCR test.
  • Seroconversion, or the development of detectable antibodies in the blood against the virus, was observed as early as 13 days post-infection.
  • The geometric mean of antibody titers of all horses ranged from 16.82-160, measured by a microneutralization assay.
  • Sequencing of the virus isolated in embryonated chicken eggs showed no adaptive mutations, indicating that IDV can replicate in horses without changing its genetic composition.

Implications

  • The findings suggest that IDV is infectious and immunogenic in horses, as shown by the virus shedding and the development of antibodies.
  • However, it doesn’t necessarily cause disease or visible symptoms in horses, as none of the studied individuals showed clinical signs of infection.
  • The ability to replicate in horses without any adaptive mutations points to the risk of interspecies transmission of IDV, particularly from cattle, a known reservoir, to horses.

Cite This Article

APA
Sreenivasan CC, Uprety T, Reedy SE, Temeeyasen G, Hause BM, Wang D, Li F, Chambers TM. (2022). Experimental Infection of Horses with Influenza D Virus. Viruses, 14(4). https://doi.org/10.3390/v14040661

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 14
Issue: 4

Researcher Affiliations

Sreenivasan, Chithra C
  • Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Uprety, Tirth
  • Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Reedy, Stephanie E
  • Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Temeeyasen, Gun
  • Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Hause, Ben M
  • Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Wang, Dan
  • Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Li, Feng
  • Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Chambers, Thomas M
  • Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.

MeSH Terms

  • Animals
  • Antibodies, Viral
  • Cattle
  • Horse Diseases
  • Horses
  • Influenza A Virus, H3N8 Subtype
  • Orthomyxoviridae
  • Orthomyxoviridae Infections
  • Thogotovirus

Grant Funding

  • R01 AI141889 / NIAID NIH HHS

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 8 times.
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