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Experimental reactivation of equid herpesvirus 1 (EHV 1) following the administration of corticosteroids.
Abstract: Eight ponies were experimentally infected with equid herpesvirus 1 (EHV 1) (subtype 1). All animals showed clinical and serological evidence of infection and virus was isolated from nasal swabs and leucocytes. These ponies were kept in isolation for a further three months during which time complement fixing antibody decreased at least four-fold. Following immunosuppression with dexamethasone and prednisolone subtype 1 virus was recovered from six of the eight animals within 14 days. Five of these six ponies were viraemic and three of them shed virus in nasal secretions; only four displayed significant rises in complement fixing antibody and only two in neutralising antibody. Clinical abnormalities were not detected during reactivation.
Publication Date: 1985-09-01 PubMed ID: 2996879DOI: 10.1111/j.2042-3306.1985.tb02524.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research pertains to an experiment conducted on eight ponies infected with equid herpesvirus 1 (EHV 1). Corticosteroids were administered to these ponies after a period of isolation. The study found that the virus reactivated in six of the eight ponies, with varying clinical responses.
Experiment Overview
- The researchers infected eight ponies with equid herpesvirus 1 (EHV 1), a strain of the herpes virus that specifically affects members of the Equidae (horse) family.
- Following the infection, the animals showed both clinical and serological (observable through blood tests) evidence of infection. The virus was also isolated from both nasal swabs and white blood cells (leucocytes).
- These ponies were then kept isolated for three months, during which their antibody levels to fight the virus dropped significantly. This is a likely sign that their immune system response was diminishing.
Corticosteroid Administration and Results
- Following the isolation period, the ponies were immunosuppressed using the corticosteroids dexamethasone and prednisolone. This was done to reduce their immune system’s response and create a more conducive environment for the virus to reactivate.
- After 14 days of the corticosteroids administration, the subtype 1 virus was found to have reactivated in six out of the eight animals. This implies that the administration of these corticosteroids may facilitate the reactivation of the EHV 1 virus in infected animals.
- However, the reactivation did not manifest uniformly in all ponies. Five of the six reactivated ponies were viraemic, meaning the virus was present in their blood. Among these, three were shedding the virus in nasal secretions, indicating active viral replication and potential for spreading the infection.
- Only four of the six reactivated ponies showed significant increases in complement fixing antibodies, a type of immune response. An even lesser number, two ponies, showed an increased presence of neutralising antibodies, antibodies that neutralise the activity of the virus.
- Interestingly, none of the six reactivated ponies showed observable clinical abnormalities. This suggests that reactivation of the virus doesn’t necessarily correlate with visible signs of illness.
Cite This Article
APA
Edington N, Bridges CG, Huckle A.
(1985).
Experimental reactivation of equid herpesvirus 1 (EHV 1) following the administration of corticosteroids.
Equine Vet J, 17(5), 369-372.
https://doi.org/10.1111/j.2042-3306.1985.tb02524.x Publication
Researcher Affiliations
MeSH Terms
- Animals
- Dexamethasone / pharmacology
- Herpesviridae Infections / microbiology
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Equid / drug effects
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases / microbiology
- Horses / microbiology
- Prednisolone / pharmacology
- Virus Activation / drug effects
Citations
This article has been cited 17 times.- Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Roberts HC, Padalino B, Pasquali P, Spoolder H, Ståhl K, Calvo AV, Viltrop A, Winckler C, Carvelli A, Paillot R, Broglia A, Kohnle L, Baldinelli F, Van der Stede Y. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus-1. EFSA J 2022 Jan;20(1):e07036.
- El-Hage C, Mekuria Z, Dynon K, Hartley C, McBride K, Gilkerson J. Association of Equine Herpesvirus 5 with Mild Respiratory Disease in a Survey of EHV1, -2, -4 and -5 in 407 Australian Horses. Animals (Basel) 2021 Nov 30;11(12).
- Sutton G, Thieulent C, Fortier C, Hue ES, Marcillaud-Pitel C, Pléau A, Deslis A, Guitton E, Paillot R, Pronost S. Identification of a New Equid Herpesvirus 1 DNA Polymerase (ORF30) Genotype with the Isolation of a C(2254)/H(752) Strain in French Horses Showing no Major Impact on the Strain Behaviour. Viruses 2020 Oct 13;12(10).
- Jerele S, Davis E, Mapes S, Pusterla N, Navas González FJ, Iglesias Pastrana C, Abdelfattah EM, McLean A. Survey of Serum Amyloid A and Bacterial and Viral Frequency Using qPCR Levels in Recently Captured Feral Donkeys from Death Valley National Park (California). Animals (Basel) 2020 Jun 23;10(6).
- Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
- Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity. BMC Vet Res 2018 Aug 22;14(1):245.
- Schulman M, Becker A, Ganswindt S, Guthrie A, Stout T, Ganswindt A. The effect of consignment to broodmare sales on physiological stress measured by faecal glucocorticoid metabolites in pregnant Thoroughbred mares. BMC Vet Res 2014 Jan 17;10:25.
- Goodman LB, Wimer C, Dubovi EJ, Gold C, Wagner B. Immunological correlates of vaccination and infection for equine herpesvirus 1. Clin Vaccine Immunol 2012 Feb;19(2):235-41.
- Chesters PM, Allsop R, Purewal A, Edington N. Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia. J Virol 1997 May;71(5):3437-43.
- Tewari D, Gibson JS, Slater JD, O'Neill T, Hannant D, Allen GP, Field HJ. Modulation of the serological response of specific pathogen-free (EHV-free) foals to EHV-1 by previous infection with EHV-4 or a TK-deletion mutant of EHV-1. Arch Virol 1993;132(1-2):101-20.
- Browning GF, Ficorilli N, Studdert MJ. Asinine herpesvirus genomes: comparison with those of the equine herpesviruses. Arch Virol 1988;101(3-4):183-90.
- Hughes CS, Gaskell RM, Jones RC, Bradbury JM, Jordan FT. Effects of certain stress factors on the re-excretion of infectious laryngotracheitis virus from latently infected carrier birds. Res Vet Sci 1989 Mar;46(2):274-6.
- Field HJ, Awan AR. Effective chemotherapy of equine herpesvirus 1 by phosphonylmethoxyalkyl derivatives of adenine demonstrated in a novel murine model for the disease. Antimicrob Agents Chemother 1990 May;34(5):709-17.
- Field HJ, Awan AR, de la Fuente R. Reinfection and reactivation of equine herpesvirus-1 in the mouse. Arch Virol 1992;123(3-4):409-19.
- Gibson JS, Slater JD, Awan AR, Field HJ. Pathogenesis of equine herpesvirus-1 in specific pathogen-free foals: primary and secondary infections and reactivation. Arch Virol 1992;123(3-4):351-66.
- Holmes CM, Wagner B. Characterization of Nasal Mucosal T Cells in Horses and Their Response to Equine Herpesvirus Type 1. Viruses 2024 Sep 25;16(10).
- Holmes CM, Babasyan S, Eady N, Schnabel CL, Wagner B. Immune horses rapidly increase antileukoproteinase and lack type I interferon secretion during mucosal innate immune responses against equine herpesvirus type 1. Microbiol Spectr 2024 Oct 3;12(10):e0109224.
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