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Home / Equine Research Bank / Equine Vet J / Exploring the genetics underpinning dynamic laryngeal collap...
Equine veterinary journal2019; 52(2); 174-180; doi: 10.1111/evj.13171

Exploring the genetics underpinning dynamic laryngeal collapse associated with poll flexion in Norwegian-Swedish Coldblooded Trotter racehorses.

Abstract: Dynamic laryngeal collapse (DLC) associated with poll flexion is the most common disorder of the upper respiratory tract (URT) in the Norwegian-Swedish Coldblooded Trotter (NSCT). The disorder, which has also been diagnosed in other breeds of trotters and gaited horses, appears to be related to anatomic phenotypes and only occurs during poll flexion when the horse is exercised 'on the bit'. Objective: Identify genomic regions associated with DLC in the NSCT by combining a rigorous phenotyping protocol with genomic data from a high-density equine genotyping array. Methods: Prospective case/control study. Methods: High-speed treadmill endoscopy was used to phenotype horses (n = 61) for DLC, distinguishing between cases and controls. Genome-wide association (GWA) analysis of DLC status was then performed using a principal component approach (PCA) with haplotype analyses subsequently performed for regions containing single-nucleotide polymorphisms (SNPs) above the suggestive genome-wide significance (GWS) threshold (P<1.0 × 10 ). Results: One region containing 10 SNPs (Equus caballus chromosome [ECA] 7: 89,601,935-94,647,192) was above the suggestive GWS threshold. Two inferred haplotypes in this region demonstrated significant differences (P<0.001) between cases and controls, with the most frequent haplotype resulting in a significantly increased risk of DLC. Conclusions: Small sample size as a result of stringent phenotyping protocols. Conclusions: The current study highlights a candidate genomic region on ECA7 as potentially important with regard to the manifestation of DLC. Further exploration of this region and the genes included within it will bring veterinarians and researchers closer to fully understanding the biological mechanisms underlying DLC in horses.
© 2019 EVJ Ltd.
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Publication Date: 2019-09-23 PubMed ID: 31461557DOI: 10.1111/evj.13171Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to identify and understand the genetic regions linked to a common upper respiratory tract disorder known as Dynamic Laryngeal Collapse (DLC) in Norwegian-Swedish Coldblooded Trotter racehorses. The team used high-speed treadmill endoscopy and genome-wide association analyses, concluding that a specific genomic region might be significant in causing DLC.

Research Objective

  • The primary objective of this research was to identify the genomic regions that could be associated with Dynamic Laryngeal Collapse (DLC), a common upper respiratory tract disorder among Norwegian-Swedish Coldblooded Trotter (NSCT) racehorses. This was achieved by combining rigorous phenotyping protocols with genetic data from high-density equine genotyping arrays.

Methods of the Study

  • A high-speed treadmill endoscopy was used to phenotype horses (n = 61) for DLC to differentiate between cases and controls.
  • A genome-wide association (GWA) analysis of DLC status was carried out using a principal component approach (PCA).
  • This was followed by haplotype analyses for regions containing single-nucleotide polymorphisms (SNPs) that were above the suggestive genome-wide significance (GWS) threshold.

Results of the Study

  • An area on Equus caballus chromosome 7 (ECA7), containing ten SNPs, exceeded the suggestive GWS threshold.
  • Two inferred haplotypes in this particular region indicated significant differences between infected and non-infected horses, with the most frequent haplotype significantly increasing the risk of DLC.

Conclusions of the Study

  • The authors acknowledged the constraints of the study, with the small sample size being a direct result of the largely strict phenotyping protocols.
  • Nevertheless, the research shed essential insights into our understanding of the genomic regions impacting DLC manifestation.
  • The identified candidate genomic region on ECA7 is crucial and needs further investigation along with the genes included within it. Such exploration will enhance our understanding of the biological mechanisms underlying DLC in horses.

Cite This Article

APA
Velie BD, Smith PM, Fjordbakk CT, Solé M, Jäderkvist Fegraeus K, Rosengren MK, Røed KH, Ihler CF, Lindgren G, Strand E. (2019). Exploring the genetics underpinning dynamic laryngeal collapse associated with poll flexion in Norwegian-Swedish Coldblooded Trotter racehorses. Equine Vet J, 52(2), 174-180. https://doi.org/10.1111/evj.13171

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 52
Issue: 2
Pages: 174-180

Researcher Affiliations

Velie, B D
  • Faculty of Science, School of Life and Environmental Sciences, University of Sydney, Sydney, Australia.
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Smith, P M
  • Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo, Norway.
Fjordbakk, C T
  • Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo, Norway.
Solé, M
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Jäderkvist Fegraeus, K
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Rosengren, M K
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Røed, K H
  • Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Oslo, Norway.
Ihler, C F
  • Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo, Norway.
Lindgren, G
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Livestock Genetics, Department of Biosystems, KU Leuven, Leuven, Belgium.
Strand, E
  • Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo, Norway.

MeSH Terms

  • Animals
  • Genome-Wide Association Study / veterinary
  • Horse Diseases
  • Horses
  • Larynx
  • Norway
  • Prospective Studies
  • Sweden

Grant Funding

  • H-15-47-075 / Swedish-Norwegian Foundation for Equine Research

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Citations

This article has been cited 2 times.
  1. Tilley P, Simões J, Sales Luis JP. Effects of a 15° Variation in Poll Flexion during Riding on the Respiratory Systems and Behaviour of High-Level Dressage and Show-Jumping Horses. Animals (Basel) 2023 May 22;13(10).
    doi: 10.3390/ani13101714pubmed: 37238147google scholar: lookup
  2. Finno CJ. Science-in-brief: Genomic and transcriptomic approaches to the investigation of equine diseases. Equine Vet J 2022 Mar;54(2):444-448.
    doi: 10.1111/evj.13549pubmed: 35133024google scholar: lookup
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