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Genes & genomics2019; 41(4); 459-465; doi: 10.1007/s13258-018-00778-3

Expression analysis of miR-221-3p and its target genes in horses.

Abstract: A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution. We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues. We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR). Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene. Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.
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Publication Date: 2019-01-02 PubMed ID: 30604147DOI: 10.1007/s13258-018-00778-3Google Scholar: Lookup
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  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the expression patterns of miR-221-3p, a microRNA, and its target gene CDKN1C in various horse tissues, using both bioinformatics and quantitative polymerase chain reaction (qPCR) tests.

MicroRNA and its Role

  • A microRNA (miRNA) is a type of non-coding RNA molecule, typically about 20 nucleotides long. It plays a crucial role in regulating gene expression in various biological processes. They perform this role by affecting the mRNA (messenger RNA) either through cleavage or by inhibiting its translation.

Horses and miRNA

  • Horses (Equus caballus), due to their applications like racing and heavy labour, have been domesticated and bred over generations leading to extensive genetic diversity. This makes them ideal subjects for studying evolution and genetic properties.
  • The study focuses on the miR-221-3p microRNA and its target gene CDKN1C in horse tissues.

Methodology

  • Target genes and seed regions of miR-221-3p were analyzed using bioinformatics tools, which also assisted in understanding the evolutionary conservation of miR-221-3p.
  • The expression patterns of miR-221-3p and its target gene CDKN1C in different horse tissues were determined using the quantitative polymerase chain reaction (qPCR) technique.

Findings

  • Out of eight horse tissues tested, miR-221-3p was found to be highly expressed in the cerebellum and spleen. In contrast, the CDKN1C gene was prevalent only in the medulla.
  • The study provides valuable expression data of miR-221-3p and the CDKN1C gene in horses, which can form the basis of future research to understand their functional importance better.

Cite This Article

APA
Kim SW, Jo A, Im J, Lee HE, Kim HS. (2019). Expression analysis of miR-221-3p and its target genes in horses. Genes Genomics, 41(4), 459-465. https://doi.org/10.1007/s13258-018-00778-3

Publication

Genes & genomics
ISSN: 2092-9293
NlmUniqueID: 101481027
Country: Korea (South)
Language: English
Volume: 41
Issue: 4
Pages: 459-465

Researcher Affiliations

Kim, So-Won
  • Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
  • Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea.
Jo, Ara
  • Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
  • Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea.
Im, Jennifer
  • Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
Lee, Hee-Eun
  • Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
  • Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea.
Kim, Heui-Soo
  • Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea. khs307@pusan.ac.kr.
  • Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea. khs307@pusan.ac.kr.

MeSH Terms

  • Animals
  • Cerebellum / metabolism
  • Conserved Sequence
  • Cyclin-Dependent Kinase Inhibitor p57 / genetics
  • Cyclin-Dependent Kinase Inhibitor p57 / metabolism
  • Evolution, Molecular
  • Horses / genetics
  • Medulla Oblongata / metabolism
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Spleen / metabolism

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