Expression of a chemokine by ciliary body epithelium in horses with naturally occurring recurrent uveitis and in cultured ciliary body epithelial cells.
Abstract: To determine whether a chemokine (RANTES)-like protein expressed by ciliary epithelium plays a role in uveitis. Methods: 3 clinically normal horses intradermal, 5 eyes from 5 horses with recurrent uveitis, and 10 normal eyes from 5 age- and sex-matched horses. Methods: Cross-reactivity and sensitivity of recombinant human (rh)-regulated upon activation, normal T-cell expressed and secreted (RANTES) protein were evaluated in horses by use of intradermal hypersensitivity reactions and a chemotaxis assay. Aqueous humor and ciliary body of eyes from clinically normal horses and horses with uveitis were examined for RANTES expression by use of an ELISA and reverse transcription-polymerase chain reaction (RT-PCR). Expression of RANTES mRNA and protein content of primary cultures of equine ciliary pigmented epithelial cells (RT-PCR) and culture supernatant (ELISA) were measured 6 or 24 hours, respectively, after cultures were stimulated with interleukin-1beta and tumor necrosis factor-alpha. Results: Strong reactions to intradermal hypersensitivity testing and significant chemotaxis of equine leukocytes to rh-RANTES wereas observed. Aqueous humor of eyes from horses with uveitis contained increased concentrations of rh-RANTES-like protein (mean +/- SD, 45.9+/-31.7 pg/ml), compared with aqueous humor from clinically normal horses (0 pg/ml). Ciliary body from horses with uveitis expressed RANTES mRNA, whereas ciliary body from clinically normal horses had low mRNA expression. Stimulated ciliary pigmented epithelial cells expressed increased amounts of rh-RANTES-like protein (506.1+/-298.3 pg/ml) and mRNA, compared with unstimulated samples. Conclusions: Ciliary epithelium may play a role in recruitment and activation of leukocytes through expression of RANTES.
Publication Date: 2002-07-18 PubMed ID: 12118672DOI: 10.2460/ajvr.2002.63.942Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article evaluates the role of a chemokine protein expressed by ciliary epithelium in uveitis, an inflammation of the eye, in horses. The study showed that horses with uveitis had higher concentrations of this protein and that the protein could contribute to the recruitment and activation of white blood cells.
Research Purpose
- This research aimed to determine the role of a chemokine, RANTES, in uveitis (an inflammation of the eye) in horses. The expression and impact of RANTES protein were assessed in normal horses and those with recurrent uveitis.
Methods
- The researchers involved three groups of horses: normal horses, horses diagnosed with recurrent uveitis, and normal horses of the same age and sex for comparison.
- The hypersensitivity reactions and the chemotactic activity of the recombinant human-regulated upon activation, normal T-cell expressed and secreted (rh-RANTES) protein were examined in horses.
- The researchers used enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) to investigate RANTES expression in the aqueous humor and ciliary body of the eyes of both normal horses and those with uveitis.
- The research also examined the production of RANTES mRNA and protein contents in primary cultures of equine ciliary pigmented epithelial cells, following stimulation with interleukin-1beta and tumor necrosis factor-alpha.
Results
- The study showed strong intradermal hypersensitivity reactions and significant chemotaxis (movement of cells in response to chemical stimuli) of horse leukocytes to rh-RANTES.
- Elevated levels of the rh-RANTES-like protein were observed in the aqueous humor of eyes from horses with uveitis, compared to those from clinically normal horses.
- Horses with uveitis showed RANTES mRNA expression in the ciliary body, while normal horses showed low expression.
- Stimulated ciliary pigmented epithelial cells showed an increase in rh-RANTES-like protein and mRNA expression, compared to unstimulated samples.
Conclusion
- The research concluded that ciliary epithelium could play a role in the recruitment and activation of leukocytes (white blood cells) through the expression of RANTES. This could further clarify the pathogenesis of uveitis and could potentially lead to new therapeutic approaches.
Cite This Article
APA
Gilger BC, Yang P, Salmon JH, Jaffe GJ, Allen JB.
(2002).
Expression of a chemokine by ciliary body epithelium in horses with naturally occurring recurrent uveitis and in cultured ciliary body epithelial cells.
Am J Vet Res, 63(7), 942-947.
https://doi.org/10.2460/ajvr.2002.63.942 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
MeSH Terms
- Animals
- Aqueous Humor / metabolism
- Chemokine CCL5 / biosynthesis
- Chemokine CCL5 / genetics
- Chemotaxis
- Ciliary Body / cytology
- Ciliary Body / metabolism
- Epithelial Cells / metabolism
- Female
- Horse Diseases / metabolism
- Horses
- Male
- RNA / chemistry
- RNA / genetics
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Sequence Analysis, DNA
- Uveitis / metabolism
- Uveitis / veterinary
Grant Funding
- 5P30EY05722 / NEI NIH HHS
- EY09106 / NEI NIH HHS
- EY11364 / NEI NIH HHS
Citations
This article has been cited 4 times.- Jia S, Chen F, Wang H, Kesavamoorthy G, Lai JS, Wong IY, Chiu K, Chan JC. Effect of Vitamin D3 on Regulating Human Tenon's Fibroblasts Activity. Transl Vis Sci Technol 2021 Jul 1;10(8):7.
- Crabtree E, Song L, Llanga T, Bower JJ, Cullen M, Salmon JH, Hirsch ML, Gilger BC. AAV-mediated expression of HLA-G1/5 reduces severity of experimental autoimmune uveitis. Sci Rep 2019 Dec 27;9(1):19864.
- Kulbrock M, Lehner S, Metzger J, Ohnesorge B, Distl O. A genome-wide association study identifies risk loci to equine recurrent uveitis in German warmblood horses. PLoS One 2013;8(8):e71619.
- Stafford LS, Plummer CE, Smith WC, Gibson DJ, Sharma J, Vicuna V, Diakite S, Larkin J 3rd. A peptide mimic of SOCS1 modulates equine peripheral immune cells in vitro and ocular effector functions in vivo: implications for recurrent uveitis. Front Immunol 2024;15:1513157.
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