Expression of endothelin in equine laminitis.
Abstract: Biosynthesis of endothelin-1 (ET-1), the most potent endogenous vasoconstrictor yet identified, is increased following myocardial infarction (MI) in man. Pathological events which occur in the connective tissues of the equine hoof during laminitis are similar in some respects, to changes occurring in the myocardial connective tissues following MI in man. The objective of this study was to determine whether ET-1 expression in connective tissues obtained from the hoof of laminitic horses is increased compared with tissues obtained from healthy horses. Expression of ET-1 in connective tissues of the equine hoof was measured following tissue extraction from 3 groups of horses: horses in which acute laminitis had been induced by the administration of starch; chronically foundered horses; nonlaminitic horses. The concentration of ET-1 in laminar connective tissues obtained from all laminitic horses (1573.0 +/- 392.8 pg/g of tissue; n = 10) was increased when compared with tissues obtained from nonlaminitic horses (392.5 +/- 117.4 pg/g of tissue; n = 5) (P<0.05). The concentration of ET-1 in laminar connective tissues obtained from the experimentally induced, acute laminitic horses (1043.6 +/- 254.4 pg/g of tissue; n = 7) and from the spontaneously affected, chronic laminitic horses (2808.3 +/- 878.6 pg/g of tissue; n = 3) was increased compared with the control group (P<0.05, P<0.01, respectively). The concentration of ET-1 in laminar connective tissues obtained from the chronic laminitic horses was greater than that of the experimentally induced, acute laminitic group (P<0.05). It is suggested that the data provide a strong argument that increased ET-1 expression in the connective tissues of the equine hoof represent a potentially important and hitherto unrecognised component of the pathophysiology of equine laminitis. Further studies are needed to determine whether inhibitors of ET-1 converting enzyme or antagonists of ET-1 receptors might be useful in the treatment and prevention of laminitis in horses.
Publication Date: 1999-07-13 PubMed ID: 10402139DOI: 10.1111/j.2042-3306.1999.tb03180.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the elevated expression of Endothelin-1, a potent vasoconstrictor, in horses with laminitis, a painful condition affecting the horse’s hoof. The findings suggest Endothelin-1 might play a significant role in the pathophysiology of the disease and offers potential treatment avenues.
Objectives and Methodology
- One of the primate objectives of this research was to examine the expression of Endothelin-1 (ET-1) in the connective tissues extracted from the hoof of horses suffering from laminitis. This study aimed to compare these findings against results from tissue samples of healthy horses.
- The researchers chose ET-1 for their study due to its known increased expression following a myocardial infarction in humans. Since there are some similarities between pathological changes post myocardial infarction and the changes witnessed in laminitis-affected horse hooves, it was theorized that ET-1 expression might also increase during laminitis.
- To make this comparison, tissue samples were extracted from three distinct groups of horses: horses with acute laminitis triggered by starch administration, horses with chronic laminitis, and healthy horses with no affliction.
Results
- The results showed that ET-1 concentration in laminar connective tissues from laminitic horses was significantly higher compared to those from healthy ones.
- On a more nuanced level, there were noticeable differences in ET-1 expressions between horses with acute and chronic laminitis. The concentration of ET-1 was higher in the horses with chronic laminitis compared to the ones with starch-induced, acute laminitis.
Conclusions and Implications
- The data obtained from the study suggest that increased ET-1 expression in the horse’s hoof connective tissues could be an essential, previously overlooked aspect of laminitis pathophysiology.
- The study adds to our understanding of equine laminitis and its underlying biochemical processes. It paves a way for further research to explore whether inhibitors of ET-1 converting enzyme or antagonists of ET-1 receptors could be utilized effectively for treating and preventing laminitis in horses.
Cite This Article
APA
Katwa LC, Johnson PJ, Ganjam VK, Kreeger JM, Messer NT.
(1999).
Expression of endothelin in equine laminitis.
Equine Vet J, 31(3), 243-247.
https://doi.org/10.1111/j.2042-3306.1999.tb03180.x Publication
Researcher Affiliations
- Department of Internal Medicine, School of Medicine, University of Missouri, Columbia 65211, USA.
MeSH Terms
- Acute Disease
- Animals
- Chronic Disease
- Connective Tissue / metabolism
- Endothelin-1 / biosynthesis
- Female
- Foot Diseases / metabolism
- Foot Diseases / veterinary
- Hoof and Claw / metabolism
- Horse Diseases / metabolism
- Horses
- Inflammation / metabolism
- Inflammation / veterinary
- Male
Citations
This article has been cited 3 times.- de Rebière de Pouyade G, Salciccia A, Ceusters J, Deby-Dupont G, Serteyn D, Mouithys-Mickalad A. Production of free radicals and oxygen consumption by primary equine endothelial cells during anoxia-reoxygenation. Open Biochem J 2011;5:52-9.
- Stokes AM, Venugopal CS, Hosgood G, Eades SC, Moore RM. Comparison of 2 endothelin-receptor antagonists on in vitro responses of equine palmar digital arterial and venous rings to endothelin-1. Can J Vet Res 2006 Jul;70(3):197-205.
- Fegraeus K, Rosengren MK, Naboulsi R, Orlando L, Åbrink M, Jouni A, Velie BD, Raine A, Egner B, Mattsson CM, Lång K, Zhigulev A, Björck HM, Franco-Cereceda A, Eriksson P, Andersson G, Sahlén P, Meadows JRS, Lindgren G. An endothelial regulatory module links blood pressure regulation with elite athletic performance. PLoS Genet 2024 Jun;20(6):e1011285.
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