Expression of platelet-derived growth factor-beta receptor and bovine papillomavirus E5 and E7 oncoproteins in equine sarcoid.
Abstract: Equine sarcoids are benign fibroblastic skin tumours that are recognized throughout the world. Infection with bovine papillomavirus (BPV) types 1 and 2 has been implicated as a major factor in disease development; however, the cellular mechanisms underlying fibroblast transformation remain poorly defined. The present study further characterizes aspects of the association with BPV in 15 equine sarcoids. BPV DNA was demonstrated in 12/15 tumours collected from different areas of Italy. Nine of these 12 tumours expressed the BPV oncoproteins E5 and E7, but these oncoproteins were not expressed by normal equine cells. The BPV E5 protein is known to bind to the platelet-derived growth factor-beta receptor (PDGF-betaR) and this molecule was expressed by 11 of the 12 sarcoids in which E5 was demonstrated. These findings add further weight to the theory that BPV and the PDGF-betaR may have a role in the pathogenesis of this disease.
Publication Date: 2008-09-23 PubMed ID: 18814884DOI: 10.1016/j.jcpa.2008.07.006Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the connection between the expression of platelet-derived growth factor-beta receptor (PDGF-betaR), and the bovine papillomavirus E5 and E7 oncoproteins in the development of equine sarcoid – a benign skin tumor common in horses. It highlights the role of bovine papillomavirus (BPV) infections, along with the cellular mechanisms in disease development.
Research Methodology
- The scope of the study involves examining the links between BPV and equine sarcoids in 15 such cases, which were retrieved from different regions in Italy.
- Emphasis was paid on examining the tumors for instances of BPV DNA. Of the 15 tumors studied, 12 showed evidence of BPV DNA presence.
- The researchers also aimed to measure the expression of BPV oncoproteins, E5 and E7, in the discovered tumors. Out of the 12 positive for BPV DNA, 9 also expressed E5 and E7 oncoproteins. Note that these oncoproteins were not expressed in normal horse cells.
Findings and Implications
- The research points at the correlation between the presence of BPV DNA and oncoproteins E5 and E7 in the development of equine sarcoids.
- The BPV E5 oncoprotein connects with the PDGF-betaR and interestingly, this molecule was found in 11 of the 12 sarcoids where E5 was expressed.
- These results suggest that BPV and PDGF-betaR might play crucial roles in causing equine sarcoids.
- These findings can be considered as important breakthroughs for deepening our understanding on the pathogenesis of equine sarcoids and may guide the development of potential treatments or preventive measures.
Cite This Article
APA
(2008).
Expression of platelet-derived growth factor-beta receptor and bovine papillomavirus E5 and E7 oncoproteins in equine sarcoid.
J Comp Pathol, 139(4), 231-237.
https://doi.org/10.1016/j.jcpa.2008.07.006 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Blotting, Western
- DNA, Viral / analysis
- Female
- Fluorescent Antibody Technique
- Horse Diseases / virology
- Horses
- Immunohistochemistry
- Male
- Microscopy, Confocal
- Oncogene Proteins, Viral / biosynthesis
- Receptor, Platelet-Derived Growth Factor beta / biosynthesis
- Skin Neoplasms / metabolism
- Skin Neoplasms / veterinary
- Skin Neoplasms / virology
Citations
This article has been cited 15 times.- Martano M, Altamura G, Power K, Liguori P, Restucci B, Borzacchiello G, Maiolino P. Beclin 1, LC3 and P62 Expression in Equine Sarcoids. Animals (Basel) 2021 Dec 23;12(1).
- Gysens L, Vanmechelen B, Haspeslagh M, Maes P, Martens A. New approach for genomic characterisation of equine sarcoid-derived BPV-1/-2 using nanopore-based sequencing. Virol J 2022 Jan 6;19(1):8.
- Koutsoumanis K, Allende A, Bolton D, Bover-Cid S, Chemaly M, Davies R, De Cesare A, Herman L, Hilbert F, Lindqvist R, Nauta M, Peixe L, Ru G, Simmons M, Skandamis P, Suffredini E, Bottari B, Cummins E, Ylivainio K, Muñoz Guajardo I, Ortiz-Pelaez A, Alvarez-Ordóñez A. Inactivation of indicator microorganisms and biological hazards by standard and/or alternative processing methods in Category 2 and 3 animal by-products and derived products to be used as organic fertilisers and/or soil improvers. EFSA J 2021 Dec;19(12):e06932.
- Martano M, Altamura G, Power K, Restucci B, Carella F, Borzacchiello G, Maiolino P. Evaluation of Hypoxia-Inducible Factor-1 Alpha (HIF-1α) in Equine Sarcoid: An Immunohistochemical and Biochemical Study. Pathogens 2020 Jan 14;9(1).
- Martano M, Power K, Restucci B, Pagano I, Altamura G, Borzacchiello G, Maiolino P. Expression of vascular endothelial growth factor (VEGF) in equine sarcoid. BMC Vet Res 2018 Sep 3;14(1):266.
- Wilson AD, Hicks C. Both tumour cells and infiltrating T-cells in equine sarcoids express FOXP3 associated with an immune-supressed cytokine microenvironment. Vet Res 2016 May 9;47(1):55.
- Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study. BMC Vet Res 2016 Feb 2;12:24.
- Altamura G, Corteggio A, Nasir L, Yuan ZQ, Roperto F, Borzacchiello G. Analysis of activated platelet-derived growth factor β receptor and Ras-MAP kinase pathway in equine sarcoid fibroblasts. Biomed Res Int 2013;2013:283985.
- Cotugno R, Gallotta D, d'Avenia M, Corteggio A, Altamura G, Roperto F, Belisario MA, Borzacchiello G. BAG3 protects bovine papillomavirus type 1-transformed equine fibroblasts against pro-death signals. Vet Res 2013 Jul 22;44(1):61.
- Corteggio A, Altamura G, Roperto F, Borzacchiello G. Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?. Infect Agent Cancer 2013 Jan 9;8(1):1.
- Altamura G, Strazzullo M, Corteggio A, Francioso R, Roperto F, D'Esposito M, Borzacchiello G. O(6)-methylguanine-DNA methyltransferase in equine sarcoids: molecular and epigenetic analysis. BMC Vet Res 2012 Nov 10;8:218.
- Strazzullo M, Corteggio A, Altamura G, Francioso R, Roperto F, D'Esposito M, Borzacchiello G. Molecular and epigenetic analysis of the fragile histidine triad tumour suppressor gene in equine sarcoids. BMC Vet Res 2012 Mar 16;8:30.
- Wobeser BK, Davies JL, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL. Epidemiology of equine sarcoids in horses in western Canada. Can Vet J 2010 Oct;51(10):1103-8.
- Ramos da Silva MA, Lima RCP, Lima ÉG, De Albuquerque BM, Gomes Nascimento DGE, Fernandes LH, De Freitas AC. Gene expression, viral load, and bovine papillomavirus coinfection in blood and cutaneous warts of cattle. Braz J Microbiol 2026 Jan 22;57(1):41.
- De Falco F, Cutarelli A, Pellicanò R, Brandt S, Roperto S. Molecular Detection and Quantification of Ovine Papillomavirus DNA in Equine Sarcoid. Transbound Emerg Dis 2024;2024:6453158.
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