Expression of the Wilms’ tumour gene and its association with PPARβ/δ in healthy skin and melanoma of horses.
Abstract: The Wilms' tumour gene (WT1) has previously been described as an oncogene in several neoplasms of humans, including melanoma, and its expression increases cancer cell proliferation. Recent reports associate the expression of the PPARβ/δ gene (peroxisome proliferator-activated receptor beta/delta) with the downregulation of WT1 in human melanoma and murine melanoma cell lines. The aim of this work was to analyse the expression of WT1 and its association with PPARβ/δ in samples of healthy and melanoma-affected skin of horses by immunohistochemistry. WT1 protein expression was detected in healthy skin, mainly in the epidermis, hair follicle, sebaceous gland and sweat gland, while no expression was observed in equine melanoma tissues. Moreover, it was observed that PPARβ/δ has a basal expression in healthy skin and that it is overexpressed in melanoma. These results were confirmed by a densitometric analysis, where a significant increase of the WT1-positive area was observed in healthy skin (128.66 ± 19.84 pixels 106) compared with that observed in melanoma (1.94 ± 0.04 pixels 106). On the other hand, a positive area with an expression of PPARβ/δ in healthy skin (214.94 ± 11.85 pixels 106) was significantly decreased compared to melanoma (624.86 ± 181.93 pixels 106). These data suggest that there could be a regulation between WT1 and PPARβ/δ in this disease in horses.
Publication Date: 2021-01-15 PubMed ID: 33459615DOI: 10.1556/004.2020.00045Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article presents a study on the Wilms’ tumour gene (WT1) and PPARβ/δ gene and their association in healthy and melanoma-affected skin in horses. The study found WT1 expression in healthy skin and PPARβ/δ overexpression in melanoma, suggesting a regulatory relationship between the two in horse melanoma.
Analysing WT1 and its Association with PPARβ/δ
- The study aims to examine the expression of the Wilms’ tumour gene (WT1) and its relationship with the PPARβ/δ gene (peroxisome proliferator-activated receptor beta/delta) in samples of healthy and melanoma-affected skin of horses.
- WT1, previously described as an oncogene in various human neoplasms including melanoma, has been associated with increased cancer cell proliferation.
- Recent studies revealed a link between the expression of the PPARβ/δ gene and the decreased expression of WT1 in human and murine melanoma cell lines.
Detection of WT1 and PPARβ/δ Expressions in Healthy and Melanoma Skin
- The expression of the WT1 protein was found in healthy equine skin, specifically in the epidermis, hair follicle, sebaceous gland, and sweat gland.
- However, no WT1 expression was detected in equine melanoma tissues.
- PPARβ/δ showed a basal expression in healthy skin and was found to be overexpressed in melanoma tissues.
Densitometric Analysis Confirming Expressions
- Densitometric analysis was performed and confirmed the occurrences of WT1 and PPARβ/δ expressions in healthy and melanoma-affected skin.
- A significant increase in the WT1-positive area was observed in healthy skin compared to that in melanoma, whereas a significant decrease in the PPARβ/δ-positive area was noted in healthy skin compared to melanoma.
Suggesting Regulatory Relationship Between WT1 and PPARβ/δ
- The observations and data from this study suggest a potential regulatory relationship between WT1 and PPARβ/δ in horse melanoma.
- While WT1 expression was characteristic of healthy skin, PPARβ/δ overexpression was associated with melanoma, indicating an inverse relationship.
Cite This Article
APA
Rangel-Sánchez IY, Salas-Treviño D, Soto-Domínguez A, Garza-Rodríguez OI, Saucedo-Cárdenas O, Zapata-Benavides P, Zarate-Ramos JJ, Cedillo-Rosales S, Zamora-Ávila DE.
(2021).
Expression of the Wilms’ tumour gene and its association with PPARβ/δ in healthy skin and melanoma of horses.
Acta Vet Hung, 68(4), 374-379.
https://doi.org/10.1556/004.2020.00045 Publication
Researcher Affiliations
- 1Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico.
- 2Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico.
- 2Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico.
- 1Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico.
- 2Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico.
- 3Northeast Biomedical Research Centre (CIBIN) of the IMSS, Monterrey, NL, Mexico.
- 4Laboratory of Immunology and Virology, Faculty of Biological Sciences, Autonomous University of Nuevo León, Mexico.
- 5Department of Parasitology, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Mexico.
- 6Department of Virology, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Mexico.
- 1Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico.
MeSH Terms
- Animals
- Genes, Wilms Tumor
- Horse Diseases
- Horses
- Melanoma / genetics
- Melanoma / veterinary
- Mice
- PPAR delta / genetics
- PPAR-beta / genetics
- Rodent Diseases
- Skin
Citations
This article has been cited 2 times.- Wagner N, Wagner KD. Peroxisome Proliferator-Activated Receptors and the Hallmarks of Cancer. Cells 2022 Aug 5;11(15).
- Zhou XY, Wang JQ, Chen JX, Chen JS. The Expression of PPAR Pathway-Related Genes Can Better Predict the Prognosis of Patients with Colon Adenocarcinoma. PPAR Res 2022;2022:1285083.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
