Fast and slow myofiber-specific expression profiles are affected by noncoding RNAs in Mongolian horses.
Abstract: The heterogeneity and plasticity of muscle fibers are essential for the athletic performance of horses, mainly at the adaption of exercises and the effect on muscle diseases. Skeletal muscle fibers can be generally distinguished by their characteristics of contraction as slow and fast type myofibers. The diversity of contractile properties and metabolism enable skeletal muscles to respond to the variable functional requirements. We investigated the muscle fiber composition and metabolic enzyme activities of splenius muscle and gluteus medius muscle from Mongolian horses. The deep RNA-seq analysis of detecting differentially expressed mRNAs, lncRNAs, circRNAs and their correlation analysis from two muscles were performed. Splenius muscle and gluteus medius muscle from Mongolian horses showed a high divergence of myofiber compositions and metabolic enzyme activities. Corresponding to their phenotypic characteristics, 57 differentially expressed long noncoding RNAs and 12 differentially expressed circle RNAs were found between two muscles. The analysis results indicate multiple binding sites were detected in lncRNAs and circRNAs with myofiber-specific expressed miRNAs. Among which we found significant correlations between the above noncoding RNAs, miRNAs, their target genes, myofiber-specific developmental transcript factors, and sarcomere genes. We suggest that the ceRNA mechanism of differentially expressed noncoding RNAs by acting as miRNA sponges could be fine tuners in regulating skeletal muscle fiber composition and transition in horses, which will operate new protective measures of muscle disease and locomotor adaption for racehorses.
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication Date: 2021-11-16 PubMed ID: 34823143DOI: 10.1016/j.cbd.2021.100942Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study explores the relationship between non-coding RNAs and the various properties of muscle fibers in Mongolian horses, revealing a possible regulatory effect on muscle fiber constitution and performance.
Understanding Muscle Fiber Heterogeneity in Horses
- The study is centered on the notion that the diversity and adaptability of muscle fibers in horses play a significant role in their athletic performance. This is particularly relevant in how the horse adapts to different physical activities and how muscle illnesses affect them.
- Horse’s skeletal muscle fibers can be broadly categorized into two groups based on their contraction properties: slow and fast type myofibers. Their distinct metabolic and contractile traits allow them to respond effectively to the varied functional requirements posed by physical activities.
- To investigate these dynamics, the researchers focused on two distinct muscles in the Mongolian Horse: the splenius muscle and gluteus medius muscle.
Deep Analysis of Muscle Fiber Composition and Metabolism
- Researchers used deep RNA-sequencing (RNA-seq) analysis to examine differentially expressed mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) across the two muscle types. The goal was to understand their correlations with each other.
- Considerable variations were found in muscle fibre composition and metabolic enzyme activities between the splenius muscle and gluteus medius muscle.
- Specifically, 57 differentially expressed lncRNAs and 12 differentially expressed circRNAs were detected between the two muscles.
Linking RNAs with Myofiber Characteristics
- An association was found between non-coding RNAs and myofiber-specific expressed microRNAs (miRNAs). Multiple binding sites were identified in both lncRNAs and circRNAs for these miRNAs.
- Furthermore, there was a noteworthy correlation between these noncoding RNAs, miRNAs, and their target genes, as well as developmental transcript factors and genes related to sarcomeric protein structures specific to muscle fibers.
Illuminating RNA’s Regulatory Role
- The analysis suggests that noncoding RNAs could function in the competing endogenous RNA (ceRNA) mechanism, acting as miRNA sponges. In this role, they potentially help fine-tune the regulation of muscle fiber composition and transition in horses.
- The findings suggest that exploring this relationship further could uncover new measures for protecting against muscle diseases and enhancing locomotive adaptation in racehorses.
Cite This Article
APA
Bou T, Han H, Mongke T, Zhao R, La X, Ding W, Jia Z, Liu H, Tiemuqier A, An T, Dugarjaviin M, Bai D.
(2021).
Fast and slow myofiber-specific expression profiles are affected by noncoding RNAs in Mongolian horses.
Comp Biochem Physiol Part D Genomics Proteomics, 41, 100942.
https://doi.org/10.1016/j.cbd.2021.100942 Publication
Researcher Affiliations
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: baidongyi1983@163.com.
MeSH Terms
- Animals
- Gene Regulatory Networks
- Horses / genetics
- MicroRNAs / genetics
- Muscle Fibers, Skeletal / metabolism
- RNA, Circular / genetics
- RNA, Long Noncoding / genetics
- RNA, Messenger / genetics
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