Fertility and 63,X Mosaicism in a Haflinger Sibship.
Abstract: Chromosomal abnormalities are notable causes of infertility in horses. Mares show various degrees of estrous behavior, and ultrasound examination often reveals an underdeveloped genital tract. This article reports investigations on fertility in a Haflinger sibship with a healthy, normally developed, fertile mare with at least three healthy offspring. Chromosomal analysis performed incidentally and blinded for this mare revealed 63,X/64,XX/65,XXX mosaicism. Two closely related mares were also mosaics (63,X/64,XX), and one of them was a carrier of a marker chromosome. Repeated examinations of the mare and seven relatives (four mares and three stallions) did not provide evidence for sub- or in-fertility. They had no developmental abnormalities or conspicuous body conditions. Peripheral blood samples were collected for analysis of the karyotype and molecular analyses. Chromosomes were Giemsa stained and 4',6-diamidino-2-phenylindole banded to identify numerical or structural aberrations of chromosomes and identification of sex chromosomes, respectively. Fluorescence in situ hybridization was performed with an equine Y-chromosome painting probe to identify and count the sex chromosomes, and polymerase chain reaction analysis was used to test for the presence of the SRY gene and investigating chimerism. The present article demonstrates the necessity of further studies analyzing chromosomal X0 mosaics to improve the predictive value of chromosomal aberrations on fertility.
Copyright © 2019 Elsevier Inc. All rights reserved.
Publication Date: 2019-05-13 PubMed ID: 31203976DOI: 10.1016/j.jevs.2019.05.008Google Scholar: Lookup
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Summary
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The research article explores the impact of chromosomal complications on mare fertility, with a specific focus on a subset of Haflinger mares. These mares exhibited chromosomal mosaicism but were found to be fertile and without developmental abnormalities, challenging common perceptions of fertility linked to chromosomal issues.
Research Context
- The research was initiated due to the common understanding that chromosomal abnormalities often lead to infertility in horses.
- The authors chose a Haflinger sibship (sibling group) for their investigations. The sibship included mares that exhibited various forms of estrous behavior and had underdeveloped genital tracts upon ultrasound examination.
The Investigation
- The researchers, using a karyotype analysis (a test to examine chromosomes for any structural anomalies), identified 63,X/64,XX/65,XXX mosaicism in a fertile mare with healthy offspring within the Haflinger sibship.
- This mare and two others related to her exhibiting 63,X/64,XX mosaicism were at the core of the study. One of these latter two mares was also carrying a marker chromosome.
- The team carried out repeated examinations on the primary mare and seven of her relatives, which included four mares and three stallions. Despite the chromosomal mosaicism, no evidence was found to indicate sub- or in-fertility.
Methodologies Employed
- Peripheral blood samples were collected from the horses and analyzed for chromosomal abnormalities. Giemsa staining and 4′,6-diamidino-2-phenylindole banding were used for this purpose.
- Additionally, fluorescence in situ hybridization was performed with an equine Y-chromosome painting probe to identify and count sex chromosomes, and a polymerase chain reaction analysis was used to test for the presence of the sex-determining region Y (SRY) gene and to analyze any potential chimerism.
Conclusions and Future Implications
- The results from this study demonstrate a need for more research to better understand X0 mosaicism and its effects on fertility.
- The authors suggest the importance of additional studies on chromosomal mosaics for enhancing the predictive value of chromosomal aberrations on fertility.
Cite This Article
APA
Neuhauser S, Handler J, Schelling C, Pieńkowska-Schelling A.
(2019).
Fertility and 63,X Mosaicism in a Haflinger Sibship.
J Equine Vet Sci, 78, 127-133.
https://doi.org/10.1016/j.jevs.2019.05.008 Publication
Researcher Affiliations
- Pferdezentrum Bad Saarow, Freie Universität Berlin, Bad Saarow, Germany.
- Pferdezentrum Bad Saarow, Freie Universität Berlin, Bad Saarow, Germany. Electronic address: johannes.handler@fu-berlin.de.
- Klinik für Reproduktionsmedizin und Zentrum für Klinische Studien, Vetsuisse Fakultät Zürich, Universität Zürich, Lindau, Switzerland.
- Klinik für Reproduktionsmedizin und Zentrum für Klinische Studien, Vetsuisse Fakultät Zürich, Universität Zürich, Lindau, Switzerland; Institut für Genetik, Vetsuisse Fakultät Bern, Universität Bern, Bern, Switzerland.
MeSH Terms
- Animals
- Female
- Fertility / physiology
- Horses / genetics
- Horses / physiology
- In Situ Hybridization, Fluorescence / veterinary
- Karyotype
- Karyotyping / veterinary
- Mosaicism / veterinary
Citations
This article has been cited 1 times.- Bugno-Poniewierska M, Raudsepp T. Horse Clinical Cytogenetics: Recurrent Themes and Novel Findings.. Animals (Basel) 2021 Mar 16;11(3).
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