Fine mapping of a quantitative trait locus for osteochondrosis on horse chromosome 2.
Abstract: In this study, we refine a quantitative trait locus for equine osteochondrosis (OC) on horse chromosome (ECA) 2 to a genome-wide significant interval at 20.08-30.94 Mb. The marker set contained 27 newly developed microsatellites equidistantly distributed over ECA2 and 44 nucleotide polymorphisms, located in 16 positional candidate genes for OC. Genotyping was performed in 211 Hanoverian horses from 14 paternal half-sib groups. A NCDN-associated SNP and haplotype were significantly associated with OC in fetlock and/or hock joints. This study is a further step towards the identification of genes responsible for OC in horses.
© 2010 The Authors, Journal compilation © 2010 Stichting International Foundation for Animal Genetics.
Publication Date: 2010-11-26 PubMed ID: 21070281DOI: 10.1111/j.1365-2052.2010.02113.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research focuses on fine-tuning the location of a genetic factor influencing the development of osteochondrosis (a joint disorder) in horses, on horse chromosome 2. The researchers identify a significant genetic interval and a specific gene associated with the development of this disorder.
Research Objectives and Methods:
- The objective of this research was to narrow down the location of a quantitative trait locus (QTL) – a section of DNA which contributes to variation in a quantitative trait – for osteochondrosis (OC) in horses on chromosome 2.
- To accomplish this, the researchers developed 27 new microsatellite markers. These are short repetitive DNA sequences used for DNA profiling and genetic mapping. They distributed these markers evenly across equine chromosome 2 (ECA2).
- Furthermore, they identified 44 nucleotide polymorphisms (changes in one base pair of a DNA sequence) located in 16 genes thought to be potential contributors to OC.
- The genetic testing using these markers and polymorphisms was conducted on 211 Hanoverian horses from 14 paternal half-sibling groups. The Hanoverian breed was chosen due the breed’s predisposition towards OC.
Results and Significance:
- The research was successful in refining the location of the QTL for OC to a significant interval. Specifically, they zeroed in on a genomic region between 20.08-30.94 million bases on ECA2. This is a considerable advancement in locating the genetic factors that contribute to OC.
- They also found a particular gene (NCDN) which demonstrated a significant association with OC when it showed certain SNP (single nucleotide polymorphism) and a specific haplotype. A haplotype is a group of genes within an organism that was inherited together from a single parent. Haplotypes can help identify the genetic variants that characterize a disease trait.
- This significant association was relevant to OC in two types of joints in horses: the fetlock and hock joints.
- This research brings scientists a step closer to identifying the specific genes responsible for the development of osteochondrosis in horses, which could have profound effects for the treatment and prevention of this debilitating disorder.
Cite This Article
APA
Dierks C, Komm K, Lampe V, Distl O.
(2010).
Fine mapping of a quantitative trait locus for osteochondrosis on horse chromosome 2.
Anim Genet, 41 Suppl 2, 87-90.
https://doi.org/10.1111/j.1365-2052.2010.02113.x Publication
Researcher Affiliations
- Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
MeSH Terms
- Animals
- Chromosomes, Mammalian
- Horse Diseases / genetics
- Horses
- Microsatellite Repeats
- Osteochondrosis / genetics
- Osteochondrosis / veterinary
- Quantitative Trait Loci
Citations
This article has been cited 8 times.- Martinez-Saez L, Marín-García PJ, Llobat ML. Osteochondrosis in horses: An overview of genetic and other factors. Equine Vet J 2026 Jan;58(1):6-19.
- Chetwynd SA, Andrews S, Inglesfield S, Delon C, Ktistakis NT, Welch HCE. Functions and mechanisms of the GPCR adaptor protein Norbin. Biochem Soc Trans 2023 Aug 31;51(4):1545-1558.
- Büttgen L, Geibel J, Simianer H, Pook T. Simulation Study on the Integration of Health Traits in Horse Breeding Programs. Animals (Basel) 2020 Jul 7;10(7).
- Raudsepp T, Finno CJ, Bellone RR, Petersen JL. Ten years of the horse reference genome: insights into equine biology, domestication and population dynamics in the post-genome era. Anim Genet 2019 Dec;50(6):569-597.
- Velie BD, Solé M, Fegraeus KJ, Rosengren MK, Røed KH, Ihler CF, Strand E, Lindgren G. Genomic measures of inbreeding in the Norwegian-Swedish Coldblooded Trotter and their associations with known QTL for reproduction and health traits. Genet Sel Evol 2019 May 27;51(1):22.
- Sevane N, Dunner S, Boado A, Cañon J. Polymorphisms in ten candidate genes are associated with conformational and locomotive traits in Spanish Purebred horses. J Appl Genet 2017 Aug;58(3):355-361.
- McCoy AM, Beeson SK, Splan RK, Lykkjen S, Ralston SL, Mickelson JR, McCue ME. Identification and validation of risk loci for osteochondrosis in standardbreds. BMC Genomics 2016 Jan 12;17:41.
- Bates JT, Jacobs JC Jr, Shea KG, Oxford JT. Emerging genetic basis of osteochondritis dissecans. Clin Sports Med 2014 Apr;33(2):199-220.
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