First case of sterility associated with sex chromosomal abnormalities in a jenny.
Abstract: Chromosomal abnormalities are one of the main causes of genetic infertility in horses. Currently, their detection rate is rising due to the use of new diagnostic tools employing molecular markers linked to the sex chromosome pair. Despite genetic similarities, there are no previous reports of sterility associated with chromosomal abnormalities in the domestic donkey (Equus asinus). Hereby, we determined the presence of a chromosomal mosaicism in a female donkey with reproductive problems using molecular methodologies developed for horses. A two-and-a-half-year-old jenny characterized by morphological abnormalities of the reproductive tract was cytogenetically analysed using conventional and fluorescent techniques and a group of microsatellite markers (short tandem repeat, STR). At the same time, five ultrasound measures of the reproductive tract were taken and compared with eight contemporary jennies of the same breed. After slaughter, morphological examinations showed that the case study had a blind vaginal vestibule defining an empty pouch that covered the entrance of the cervical os. Histopathological studies demonstrated that this abnormal structure was compatible with a remnant hymen. Molecular markers, STR and fluorescent in situ hybridization determinations revealed that the animal was a 62, XX/61,X mosaic and, therefore, the first case of chromosomal abnormalities in the sex pair reported in donkeys.
© 2016 Blackwell Verlag GmbH.
Publication Date: 2016-12-01 PubMed ID: 27905141DOI: 10.1111/rda.12884Google Scholar: Lookup
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- Journal Article
Summary
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This research documents the first known case of a female donkey, or jenny, being found sterile due to chromosomal abnormalities. The study uses molecular methodologies designed for horses to diagnose the reproductive issues in this donkey.
Background
- Chromosomal abnormalities are among the major factors causing genetic infertility in horses, and new diagnostic tools are increasing their detection rate.
- Despite genetic similarities between equines, no such instances of sterility due to chromosomal irregularities have previously been reported in donkeys (Equus asinus).
Case Study
- The focal subject of this study was a two-and-a-half-year-old, female donkey that had abnormal morphological features in her reproductive tract.
- Conventional and fluorescent cytogenetic analysis was conducted, and microsatellite markers, also known as short tandem repeat (STR) markers, were used.
- Alongside cytogenetic analysis, ultrasound measurements of the donkey’s reproductive tract were taken and compared to eight contemporary jennies of the same breed.
Findings
- Post-slaughter examinations of this donkey revealed a blind vaginal vestibule — an empty pouch covering the cervical os entrance.
- Histopathology classified this abnormal structure as a possible leftover hymen.
- Molecular markers, STR, and fluorescent in situ hybridization tests indicated a 62, XX/61,X mosaic chromosomal makeup in the donkey, thus confirming her sterility due to chromosomal abnormalities.
Significance
- This study marks the first recorded case of chromosomal abnormalities leading to sterility in a donkey, simultaneously expanding our comprehension of equine genetics and reproductive health issues.
Cite This Article
APA
Dorado J, Anaya G, Bugno-Poniewierska M, Molina A, Mendez-Sanchez A, Ortiz I, Moreno-Millán M, Hidalgo M, Peral García P, Demyda-Peyrás S.
(2016).
First case of sterility associated with sex chromosomal abnormalities in a jenny.
Reprod Domest Anim, 52(2), 227-234.
https://doi.org/10.1111/rda.12884 Publication
Researcher Affiliations
- Veterinary Reproduction Group AGR-275, Department of Animal Medicine and Surgery, Veterinary Teaching Hospital, University of Cordoba, Cordoba, Spain.
- Laboratory of Animal Genomics, MERAGEM AGR-158 Research Group, Department of Genetics, University of Córdoba, Cordoba, Spain.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Laboratory of Animal Genomics, MERAGEM AGR-158 Research Group, Department of Genetics, University of Córdoba, Cordoba, Spain.
- Department of Anatomy and Comparative Pathology, University of Cordoba, Cordoba, Spain.
- Veterinary Reproduction Group AGR-275, Department of Animal Medicine and Surgery, Veterinary Teaching Hospital, University of Cordoba, Cordoba, Spain.
- Laboratory of Applied and Molecular Animal Cytogenetics, MERAGEM AGR-158 Research Group, Department of Genetics, University of Cordoba, Cordoba, Spain.
- Veterinary Reproduction Group AGR-275, Department of Animal Medicine and Surgery, Veterinary Teaching Hospital, University of Cordoba, Cordoba, Spain.
- Facultad de Ciencias Veterinarias, IGEVET - Instituto de Genética Veterinaria UNLP - CONICET LA PLATA, Universidad Nacional de La Plata, La Plata, Argentina.
- Facultad de Ciencias Veterinarias, IGEVET - Instituto de Genética Veterinaria UNLP - CONICET LA PLATA, Universidad Nacional de La Plata, La Plata, Argentina.
MeSH Terms
- Animals
- Equidae / genetics
- Female
- Infertility, Female / genetics
- Microsatellite Repeats
- Sex Chromosome Aberrations / veterinary
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