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Home / Equine Research Bank / Pathogens / First Evidence of Ehrlichia minasensis Infection in Horses f...
Pathogens (Basel, Switzerland)2021; 10(3); 265; doi: 10.3390/pathogens10030265

First Evidence of Ehrlichia minasensis Infection in Horses from Brazil.

Abstract: The genus includes tick-borne bacterial pathogens affecting humans, domestic and wild mammals. has been identified in different animal species and geographical locations, suggesting that this is a widely distributed and generalist . In the present study, we evaluated infection in 148 Equidae presented to the Medical Clinic Department of a Veterinary Hospital from a midwestern region of Brazil. Blood samples and ticks collected from the animals were tested by Polymerase Chain Reaction (PCR) for the presence of spp. A multigenic approach including Anaplasmataceae-specific (i.e., 16S rRNA, , ) and -specific (i.e., and ) genes was used for accurate bacteria identification. Sera samples were also collected and evaluated for the detection of anti- antibodies by indirect fluorescent antibody test (IFA). Possible associations between molecular and serological diagnostics and clinical and hematological manifestations were tested using chi-squared or Fisher's exact tests. Sequence analysis of the fragment revealed that three horses (2.03%) were exposed to . Sixty-one (41.2%) Equidae (58 equines and three mules), were seropositive for spp., with antibody titers ranging between 40 and 2560. Seropositivity to ehrlichial antigens was statistically associated with tick infestation, rural origin, hypoalbuminemia and hyperproteinemia ( ≤ 0.05). The present study reports the first evidence of natural infection by in horses from Brazil.
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Publication Date: 2021-02-25 PubMed ID: 33669023PubMed Central: PMC7996482DOI: 10.3390/pathogens10030265Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about the occurrence of Ehrlichia minasensis, a tick-borne bacterial pathogen, in horses from Brazil.

Introduction

  • The study focuses on Ehrlichia minasensis, a bacterium from the genus Ehrlichia, which is primarily transmitted by ticks, affecting humans and different species of wild and domestic mammals.
  • The entity Ehrlichia minasensis has previously been identified in various animals and locations, illustrating its extensive distribution and adaptability to different hosts.

Materials and Methods

  • The study examined 148 Equidae (a family of animals that includes horses and mules), all clients of a Veterinary Hospital’s Medical Clinic Department in the midwestern region of Brazil.
  • Blood samples and ticks taken from the animals were analysed using Polymerase Chain Reaction (PCR) techniques to test for the presence of Ehrlichia spp.
  • The team used a collection of both Anaplasmataceae-specific and Ehrlichia-specific genes for accurate identification of the bacteria.
  • They also collected serum samples to check for the presence of anti-Ehrlichia antibodies through the indirect fluorescent antibody test (IFA).
  • The researchers also looked for possible connections between molecular and serological results and clinical symptoms and blood changes, through chi-squared or Fisher’s exact tests.

Results

  • Sequence analysis of the p51 fragment, a specific type of gene, showed that three horses (2.03% of the test group) had been exposed to Ehrlichia minasensis.
  • 41.2% of the studied equidae (total 61 animals, including 58 horses and 3 mules) were found seropositive for Ehrlichia spp., which means they had antibodies against Ehrlichia in their blood. The antibody titers varied between 40 and 2560.
  • Statistical analysis showed that seropositivity to ehrlichial antigens (meaning antibodies produced in response to the bacteria were present in the blood) was directly associated with tick infestation, rural origin, hypoalbuminemia (low levels of albumin protein in the blood), and hyperproteinemia (elevated overall protein level in the blood).

Conclusion

  • The study marks the first recorded incidence of natural infection by Ehrlichia minasensis in horses from Brazil.

Cite This Article

APA
Muraro LS, Souza AO, Leite TNS, Cândido SL, Melo ALT, Toma HS, Carvalho MB, Dutra V, Nakazato L, Cabezas-Cruz A, Aguiar DM. (2021). First Evidence of Ehrlichia minasensis Infection in Horses from Brazil. Pathogens, 10(3), 265. https://doi.org/10.3390/pathogens10030265

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 10
Issue: 3
PII: 265

Researcher Affiliations

Muraro, Lívia S
  • Laboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, Brazil.
Souza, Aneliza de O
  • Veterinary Clinical Laboratory, Department of Veterinary Clinics, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, Brazil.
Leite, Tamyres N S
  • Veterinary Clinical Laboratory, Department of Veterinary Clinics, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, Brazil.
Cândido, Stefhano L
  • Laboratory of Microbiology and Molecular Biology, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, Brazil.
Melo, Andréia L T
  • Veterinary of Clinical, Veterinary Medicine College, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, Brazil.
Toma, Hugo S
  • Veterinary Medicine Department, Federal University of Lavras (UFLA), Campus Universitário, Mailbox 3037, Lavras 37200-000, Brazil.
Carvalho, Mariana B
  • Veterinary of Clinical, Veterinary Medicine College, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, Brazil.
Dutra, Valéria
  • Laboratory of Microbiology and Molecular Biology, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, Brazil.
Nakazato, Luciano
  • Laboratory of Microbiology and Molecular Biology, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, Brazil.
Cabezas-Cruz, Alejandro
  • Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, F-94700 Maisons-Alfort, France.
Aguiar, Daniel M de
  • Laboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, Brazil.

Grant Funding

  • 303677/2018-0 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico
  • 301811/2015-6 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Parkinson M, Vuyyuru S, Patel J, Animalu C. Challenges of Diagnosing Severe Ehrlichiosis in Orthotopic Liver Transplant Recipients.. Case Rep Transplant 2021;2021:8285326.
    doi: 10.1155/2021/8285326pubmed: 34840851google scholar: lookup
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