First molecular detection of Equine Herpesvirus type 3 (EHV-3) in Chile.
Abstract: Equine coital rash (ECE) is a highly contagious benign infection that induces lesions on external genitals, and it is caused by the equine herpesvirus type 3 (EHV-3). Although the disease is globally distributed, its presence in Chile has not been documented from a genetic point of view. Here, we performed polymerase chain reaction screenings for EHV-3 in lesions of external genitals in four horses belonging to a riding station at Bulnes, Ñuble Region, Chile. We sequenced a fragment of the glycoprotein G (gG) gene from three horses with clinical signs of ECE. The sequences were identical between them and 99.7% similar to a haplotype of EHV-3 detected in Brazil, and phylogenetically related with homologue from Japan, Russia and Brazil. Our results show the presence of EHV-3 for the first time in horses with ECE in Chile.
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
Publication Date: 2022-10-17 PubMed ID: 36253808PubMed Central: PMC10029895DOI: 10.1002/vms3.976Google Scholar: Lookup
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- Case Reports
- Research Support
- Non-U.S. Gov't
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Disease Etiology
- Disease Outbreaks
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Herpesvirus
- Genetics
- Horses
- In Vitro Research
- In Vivo
- Infectious Disease
- Molecular biology
- Polymerase Chain Reaction
- Veterinary Medicine
- Veterinary Research
- Virology
Summary
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The research study documents the first detection of Equine Herpesvirus type 3 (EHV-3), the cause of equine coital exanthema (ECE), in Chile.
Background
- ECE is a local and highly contagious infection that causes lesions in equine (referring to horses, donkeys, and zebras) external genitals and is caused by the Equine Herpesvirus type 3 (EHV-3).
- While the disease is globally distributed, in Chile it hadn’t been documented from a genetic point of view.
Study Purpose
- The researchers conducted this study to identify through polymerase chain reaction (PCR) procedures if the EHV-3 was present in four horses in Bulnes, Ñuble Region, Chile.
Methodology
- PCR screenings for EHV-3 were performed on genital lesions of these horses using a sample of the glycoprotein G (gG) gene, a gene common to EHV-3.
- These procedures allowed the researchers to extract and analyze the DNA present in the samples to identify the presence of EHV-3.
Results
- The sequences that were extracted from three of the horses —all exhibiting clinical signs of ECE— were identical to each other.
- These sequenced fragments showed 99.7% similarity to a haplotype of EHV-3 previously detected in Brazil, indicating a strong genetic relation.
- The researchers further validated these results by comparing these sequences to homologues in Japan, Russia, and Brazil, finding a strong phylogenetic relationship.
Conclusion
- The study confirms the presence of EHV-3 in horses with ECE in Chile for the first time.
- This knowledge advances understanding of EHV-3’s global distribution and provides a base for further studies in preventing and managing ECE in Chile.
Cite This Article
APA
Troncoso I, Calvanese R, Saravia F, Muñoz-Leal S, Zegpi NA, Ortega R.
(2022).
First molecular detection of Equine Herpesvirus type 3 (EHV-3) in Chile.
Vet Med Sci, 9(2), 717-720.
https://doi.org/10.1002/vms3.976 Publication
Researcher Affiliations
- School of Veterinary Medicine, Faculty of Veterinary Medicine and Agronomy, Universidad de las Americas, Concepción, Chile.
- Faculty of Agricultury Sciences, Universidad del Alba, Chillán, Chile.
- Manager of the Equine Tracer Center, Chilean Army Agreement -INDAP, Municipality of Bulnes and San Carlos, Ñuble, Chile.
- Department of Animal Sciences, Faculty of Veterinary Science, Universidad de Concepción, Chillán, Chile.
- Department of Animal Sciences, Faculty of Veterinary Science, Universidad de Concepción, Chillán, Chile.
- Pathology Department, Faculty of Veterinary Science, Universidad de Concepción, Chillán, Chile.
- Pathology Department, Faculty of Veterinary Science, Universidad de Concepción, Chillán, Chile.
MeSH Terms
- Animals
- Horses
- Herpesvirus 3, Equid
- Chile / epidemiology
- Herpesviridae Infections / diagnosis
- Herpesviridae Infections / epidemiology
- Herpesviridae Infections / veterinary
- Polymerase Chain Reaction / veterinary
- Base Sequence
- Horse Diseases / diagnosis
- Horse Diseases / epidemiology
Conflict of Interest Statement
The authors declare no conflict of interest.
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