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Tropical animal health and production2017; 49(8); 1793-1796; doi: 10.1007/s11250-017-1384-7

First report of molecular characterization and phylogenetic analysis of RoTat 1.2 VSG of Trypanosoma evansi from equine isolate.

Abstract: Rotat 1.2 variant surface glycoprotein (VSG) is considered to be an important VSG expressed in most of the isolates of Trypanosoma evansi. This makes the molecule an important candidate for both molecular- and serological-based detection of surra. There are ample reports of existence of this gene in isolates from cattle, buffalo, and camel across the world. Of late, there are reports of its absence from a fewer isolates of T. evansi of murine and wildlife origin. Search of literature revealed no reports from horses. The present communication presents the first report of molecular cloning and characterization of Rotat 1.2 VSG from horse isolate of T. evansi from semi-arid region of India. Alongside, the gene was compared with various other isolates across the world. Interestingly, the isolate was found to be closer to camel isolates from Egypt than the other known isolates from India and Kenya.
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Publication Date: 2017-08-22 PubMed ID: 28831704DOI: 10.1007/s11250-017-1384-7Google Scholar: Lookup
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Summary

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The research article discusses the molecular characterization and comparison of the RoTat 1.2 variant surface glycoprotein (VSG) of the parasite Trypanosoma evansi, specifically isolated from horses in India. This is a significant marker for the detection of surra disease, which the parasite causes.

Introduction

  • The molecule RoTat 1.2 VSG is a crucial variant surface glycoprotein (VSG) observed to be present in isolates of Trypanosoma evansi, a parasite causing surra in animals.
  • This molecule plays a key role in molecular- and serological-based detection of surra, hence its importance.
  • Previous studies have established the existence of this molecule in isolates from cattle, buffalo, and camels.
  • Recent research has also reported its absence in a few isolates of T. evansi of murine and wildlife origins.
  • There was no existing literature available discussing the presence of this molecule in horse isolates.

Methodology and Results

  • The study presents the first instance of the molecular cloning and characterization of RoTat 1.2 VSG extracted from a horse isolate of T. evansi from a semi-arid region in India.
  • Further, the researchers compared this gene with various other isolates from around the world.

Findings and Observations

  • The comparison of the genetic material in question revealed that the isolate from the horse was more closely related to camel isolates from Egypt.
  • This comparison suggested a stronger similarity between the Indian horse isolate and the Egyptian camel ones than other known isolates from India and Kenya.
  • The results from this comparison could pave the way for new insights about T. evansi and surra in different geographical locations.

Cite This Article

APA
Sudan V, Jaiswal AK, Shanker D, Verma AK. (2017). First report of molecular characterization and phylogenetic analysis of RoTat 1.2 VSG of Trypanosoma evansi from equine isolate. Trop Anim Health Prod, 49(8), 1793-1796. https://doi.org/10.1007/s11250-017-1384-7

Publication

Tropical animal health and production
ISSN: 1573-7438
NlmUniqueID: 1277355
Country: United States
Language: English
Volume: 49
Issue: 8
Pages: 1793-1796

Researcher Affiliations

Sudan, Vikrant
  • College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India. viks.sudan@gmail.com.
Jaiswal, Amit Kumar
  • College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
Shanker, Daya
  • College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
Verma, Amit Kumar
  • College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India.

MeSH Terms

  • Animals
  • Antigens, Protozoan / genetics
  • Cattle
  • Cloning, Molecular
  • Horse Diseases / parasitology
  • Horses
  • Mice
  • Phylogeny
  • Trypanosoma / classification
  • Trypanosoma / genetics
  • Trypanosoma / isolation & purification
  • Trypanosomiasis / parasitology
  • Trypanosomiasis / veterinary

Grant Funding

  • 185/DR/2015 / DUVASU

References

This article includes 18 references
  1. Allen TE, Ullman B. Cloning and expression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma brucei.. Nucleic Acids Res 1993 Nov 25;21(23):5431-8.
    pubmed: 8265360doi: 10.1093/nar/21.23.5431google scholar: lookup
  2. Gadelha C, Holden JM, Allison HC, Field MC. Specializations in a successful parasite: what makes the bloodstream-form African trypanosome so deadly?. Mol Biochem Parasitol 2011 Oct;179(2):51-8.
    pubmed: 21763356doi: 10.1016/j.molbiopara.2011.06.006google scholar: lookup
  3. Li SQ, Fung MC, Reid SA, Inoue N, Lun ZR. Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T. evansi, T. equiperdum and T. b. brucei infection in mice.. Parasite Immunol 2007 Apr;29(4):191-9.
    pubmed: 17371456doi: 10.1111/j.1365-3024.2006.00933.xgoogle scholar: lookup
  4. Gaur RS, Sudan V, Jaiswal AK, Singh A, Shanker D. Classico-molecular targeting of oligopeptidase B, cysteine protease and variable surface glycoprotein (VSG) genes of Trypanosoma evansi.. J Parasit Dis 2017 Mar;41(1):51-54.
    pubmed: 28316387doi: 10.1007/s12639-016-0748-7google scholar: lookup
  5. Reinwald E. Role of carbohydrates within variant surface glycoprotein of Trypanosoma congolense. Protection against proteolytic attack.. Eur J Biochem 1985 Sep 2;151(2):385-91.
    pubmed: 3896788doi: 10.1111/j.1432-1033.1985.tb09113.xgoogle scholar: lookup
  6. Barry JD, McCulloch R. Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite.. Adv Parasitol 2001;49:1-70.
    pubmed: 11461029doi: 10.1016/s0065-308x(01)49037-3google scholar: lookup
  7. Rea D, Hazell C, Andrews NW, Morty RE, Fülöp V. Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei.. Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Aug 1;62(Pt 8):808-10.
    pubmed: 16880564doi: 10.1107/S1744309106027874google scholar: lookup
  8. Rogé S, Van Reet N, Odiwuor S, Tran T, Schildermans K, Vandamme S, Vandenberghe I, Vervecken W, Gillingwater K, Claes F, Devreese B, Guisez Y, Büscher P. Recombinant expression of trypanosome surface glycoproteins in Pichia pastoris for the diagnosis of Trypanosoma evansi infection.. Vet Parasitol 2013 Nov 8;197(3-4):571-9.
    pubmed: 23747105doi: 10.1016/j.vetpar.2013.05.009google scholar: lookup
  9. Li SQ, Yang WB, Ma LJ, Xi SM, Chen QL, Song XW, Kang J, Yang LZ. Immunization with recombinant actin from Trypanosoma evansi induces protective immunity against T. evansi, T. equiperdum and T. b. brucei infection.. Parasitol Res 2009 Jan;104(2):429-35.
    pubmed: 18923843doi: 10.1007/s00436-008-1216-9google scholar: lookup
  10. Sengupta PP, Balumahendiran M, Balamurugan V, Rudramurthy GR, Prabhudas K. Expressed truncated N-terminal variable surface glycoprotein (VSG) of Trypanosoma evansi in E. coli exhibits immuno-reactivity.. Vet Parasitol 2012 Jun 8;187(1-2):1-8.
    pubmed: 22277627doi: 10.1016/j.vetpar.2012.01.012google scholar: lookup
  11. Sengupta PP, Balumahendiran M, Suryanaryana VV, Raghavendra AG, Shome BR, Gajendragad MR, Prabhudas K. PCR-based diagnosis of surra-targeting VSG gene: experimental studies in small laboratory rodents and buffalo.. Vet Parasitol 2010 Jul 15;171(1-2):22-31.
    pubmed: 20388585doi: 10.1016/j.vetpar.2010.03.011google scholar: lookup
  12. Ngaira JM, Njagi EN, Ngeranwa JJ, Olembo NK. PCR amplification of RoTat 1.2 VSG gene in Trypanosoma evansi isolates in Kenya.. Vet Parasitol 2004 Feb 26;120(1-2):23-33.
    pubmed: 15019140doi: 10.1016/j.vetpar.2003.12.007google scholar: lookup
  13. Tran T, Büscher P, Vandenbussche G, Wyns L, Messens J, De Greve H. Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75.. J Biotechnol 2008 Jun 30;135(3):247-54.
    pubmed: 18538880doi: 10.1016/j.jbiotec.2008.04.012google scholar: lookup
  14. Freymann D, Down J, Carrington M, Roditi I, Turner M, Wiley D. 2.9 A resolution structure of the N-terminal domain of a variant surface glycoprotein from Trypanosoma brucei.. J Mol Biol 1990 Nov 5;216(1):141-60.
    pubmed: 2231728doi: 10.1016/S0022-2836(05)80066-Xgoogle scholar: lookup
  15. Zhang ZQ, Baltz T. Identification of Trypanosoma evansi, Trypanosoma equiperdum and Trypanosoma brucei brucei using repetitive DNA probes.. Vet Parasitol 1994 Jun;53(3-4):197-208.
    pubmed: 7975115doi: 10.1016/0304-4017(94)90183-xgoogle scholar: lookup
  16. Verloo D, Magnus E, Büscher P. General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin.. Vet Parasitol 2001 Jun 12;97(3):183-9.
    pubmed: 11390070doi: 10.1016/s0304-4017(01)00412-5google scholar: lookup
  17. Verloo D, Holland W, My LN, Thanh NG, Tam PT, Goddeeris B, Vercruysse J, Büscher P. Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam.. Vet Parasitol 2000 Sep 20;92(2):87-96.
    pubmed: 10946132doi: 10.1016/s0304-4017(00)00284-3google scholar: lookup
  18. Salim B, Bakheit MA, Kamau J, Nakamura I, Sugimoto C. Molecular epidemiology of camel trypanosomiasis based on ITS1 rDNA and RoTat 1.2 VSG gene in the Sudan.. Parasit Vectors 2011 Mar 4;4:31.
    pubmed: 21375725doi: 10.1186/1756-3305-4-31google scholar: lookup

Citations

This article has been cited 2 times.
  1. Boutellis A, Bellabidi M, Benaissa MH, Harrat Z, Brahmi K, Drali R, Kernif T. New Haplotypes of Trypanosoma evansi Identified in Dromedary Camels from Algeria.. Acta Parasitol 2021 Mar;66(1):294-302.
    doi: 10.1007/s11686-020-00316-wpubmed: 33389544google scholar: lookup
  2. Gaur RS, Shanker D, Sudan V, Paliwal S, Singh S, Jadaun A. Associative Genetic Diversity of RoTat 1.2 VSG in Different Trypanosoma evansi Isolates.. Acta Parasitol 2021 Mar;66(1):199-204.
    doi: 10.1007/s11686-020-00273-4pubmed: 32944813google scholar: lookup
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