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Animals : an open access journal from MDPI2024; 14(4); 598; doi: 10.3390/ani14040598

First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy.

Abstract: Equine influenza (EI) is a highly contagious viral disease of equids characterized by pyrexia and respiratory signs. Like other influenza A viruses, antigenic drift or shift could lead to a vaccine-induced immunity breakdown if vaccine strains are not updated. The aim of this study was to genetically characterize EIV strains circulating in Italy, detected in PCR-positive samples collected from suspected cases, especially in the absence of formal active surveillance. Methods: Between February and April 2019, blood samples and nasal swabs collected from each of the 20 symptomatic horses from North and Central Italy were submitted to the National Reference Centre for Equine Diseases in Italy to confirm preliminary analysis performed by other laboratories. Results: None of the sera analysed using haemagglutination inhibition and single radial haemolysis presented a predominant serological reactivity pattern for any antigen employed. All nasal swabs were positive with IAV RRT-PCR. Only one strain, isolated in an embryonated chicken egg from a sample collected from a horse of a stable located in Brescia, Lombardy, was identified as H3N8 Florida lineage clade 1 (FC1). In the constructed phylogenetic trees, this strain is located within the FC1, together with the virus isolated in France in 2018 (MK501761). Conclusions: This study reports the first detection of H3N8 FC1 in Italy, highlighting the importance of monitoring circulating EIV strains to verify the vaccine composition appropriateness for maximum efficacy.
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Publication Date: 2024-02-12 PubMed ID: 38396566PubMed Central: PMC10886299DOI: 10.3390/ani14040598Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Equine influenza virus (EIV) strains circulating in Italy were genetically characterized, and for the first time, the H3N8 Florida Clade 1 (FC1) virus was detected in horses, emphasizing the need for ongoing surveillance to ensure vaccine effectiveness.

Introduction to Equine Influenza and Study Purpose

  • Equine influenza (EI) is a contagious viral disease affecting horses and related animals, primarily causing fever and respiratory symptoms.
  • The virus responsible, equine influenza A virus (EIV), can mutate through processes called antigenic drift or shift, potentially reducing the effectiveness of existing vaccines.
  • This study aimed to genetically analyze EIV strains present in Italy, especially since there was no formal active surveillance in place at the time, to understand which virus variants are infecting horses.

Methods and Sample Collection

  • Between February and April 2019, researchers collected blood samples and nasal swabs from 20 symptomatic horses located in North and Central Italy.
  • Samples were submitted to the National Reference Centre for Equine Diseases, the official body in Italy for confirming preliminary results from other laboratories.
  • Blood sera were tested via haemagglutination inhibition (HI) and single radial haemolysis (SRH) assays to detect antibody responses to different EIV antigens.
  • Nasal swabs underwent real-time reverse transcription PCR (RRT-PCR) to detect influenza A virus (IAV) genomic material.
  • Virus isolation attempts were made by inoculating samples into embryonated chicken eggs, a common technique used to culture influenza viruses.

Results Overview

  • Serological tests (HI and SRH) did not reveal a dominant antibody response pattern to any of the tested influenza antigens in the sera, indicating a complex or mixed antibody response or recent infections.
  • All nasal swabs tested positive for influenza A virus RNA by RRT-PCR, confirming active infection in those horses.
  • Only one virus strain was successfully isolated from a nasal swab of a horse in Brescia, Lombardy.
  • This isolate was genetically identified as belonging to the H3N8 Florida lineage clade 1 (FC1), marking the first ever detection of this virus clade in Italy.

Genetic and Phylogenetic Analysis

  • Phylogenetic trees were constructed using genetic sequences to classify the isolated strain.
  • The Italian isolate clustered closely with the FC1 virus that had been isolated in France in 2018, indicating possible regional circulation or recent spread within Europe.

Conclusions and Implications

  • This is the first report of H3N8 Florida Clade 1 virus in horses from Italy, expanding the known presence of this virus lineage in Europe.
  • The detection emphasizes the necessity of continuous monitoring and genetic characterization of equine influenza viruses to track their evolution.
  • Regular surveillance information is critical for verifying whether the strains included in vaccines are up to date and capable of providing effective protection against circulating viruses.
  • Maintaining effective vaccines reduces the risk of outbreaks and severe disease in horse populations.

Cite This Article

APA
(2024). First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy. Animals (Basel), 14(4), 598. https://doi.org/10.3390/ani14040598

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 4
PII: 598

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 35 references
  1. Chambers TM. Equine Influenza. Cold Spring Harb. Perspect. Med. 2022;12:a038331.
    doi: 10.1101/cshperspect.a038331pmc: PMC8725621pubmed: 32152243google scholar: lookup
  2. Nemoto M, Ohta M, Yamanaka T, Kambayashi Y, Bannai H, Tsujimura K, Yamayoshi S, Kawaoka Y, Cullinane A. Antigenic differences between equine influenza virus vaccine strains and Florida sublineage clade 1 strains isolated in Europe in 2019. Vet. J. 2021;272:105674.
    doi: 10.1016/j.tvjl.2021.105674pubmed: 33941332google scholar: lookup
  3. Oladunni FS, Oseni SO, Martinez-Sobrido L, Chambers TM. Equine influenza virus and vaccines. Viruses 2021;13:1657.
    doi: 10.3390/v13081657pmc: PMC8402878pubmed: 34452521google scholar: lookup
  4. Alves Beuttemmüller E, Woodward A, Rash A, Dos Santos Ferraz LE, Fernandes Alfieri A, Alfieri AA, Elton D. Characterisation of the epidemic strain of H3N8 equine influenza virus responsible for outbreaks in South America in 2012. Virol. J. 2016;13:45.
    doi: 10.1186/s12985-016-0503-9pmc: PMC4799594pubmed: 26993620google scholar: lookup
  5. Olguin-Perglione C, Barrandeguy ME. An overview of equine influenza in south america. Viruses 2021;13:888.
    doi: 10.3390/v13050888pmc: PMC8151294pubmed: 34065839google scholar: lookup
  6. Rash A. Diagnosis of equine influenza. Vet. Rec. 2017;181:113–114.
    doi: 10.1136/vr.j3459pubmed: 28754735google scholar: lookup
  7. Virmani N, Singh BK, Gulati BR, Kumar S. Equine influenza outbreak in India. Vet. Rec. 2008;163:607–608.
    doi: 10.1136/vr.163.20.607-apubmed: 19011253google scholar: lookup
  8. Paillot R, Pitel PH, Pronost S, Legrand L, Fougerolle S, Jourdan M, Marcillaud-Pitel C. Florida clade 1 equine influenza virus in France. Vet. Rec. 2019;184:101.
    doi: 10.1136/vr.l1203pubmed: 30655407google scholar: lookup
  9. Whitlock F, Murcia PR, Newton JR. A Review on Equine Influenza from a Human Influenza Perspective. Viruses 2022;14:1312.
    doi: 10.3390/v14061312pmc: PMC9229935pubmed: 35746783google scholar: lookup
  10. Walker-Panse L, Rash A, Huckstep J, Payne S, Blake S, Whitlock F, Elton D, Newton R, Bryant NA. Equine influenza virus surveillance in the United Kingdom from 2019 to 2021. Equine Vet. J. 2021:78–79.
    doi: 10.1111/evj.122_13495google scholar: lookup
  11. Damiani AM, Scicluna MT, Ciabatti I, Cardeti G, Sala M, Vulcano G, Cordioli P, Martella V, Amaddeo D, Autorino GL. Genetic characterization of equine influenza viruses isolated in Italy between 1999 and 2005. Virus Res. 2008;131:100–105.
    doi: 10.1016/j.virusres.2007.08.001pubmed: 17889395google scholar: lookup
  12. Bryant NA, Rash AS, Russell CA, Ross J, Cooke A, Bowman S, MacRae S, Lewis NS, Paillot R, Zanoni R. Antigenic and genetic variations in European and North American equine influenza virus strains (H3N8) isolated from 2006 to 2007. Vet. Microbiol. 2009;138:41–52.
    doi: 10.1016/j.vetmic.2009.03.004pubmed: 19346084google scholar: lookup
  13. Legrand LJ, Pitel PHY, Cullinane AA, Fortier GD, Pronost SL. Genetic evolution of equine influenza strains isolated in France from 2005 to 2010. Equine Vet. J. 2015;47:207–211.
    doi: 10.1111/evj.12244pubmed: 25763436google scholar: lookup
  14. Autorino GL, Frontoso R, Cersini A, Manna G, Damiani AM, Scicluna MT. Case Report of Equine Influenza in Italy, in 2014. J. Equine Vet. Sci. 2015;35:779–783.
    doi: 10.1016/j.jevs.2015.06.020google scholar: lookup
  15. Elton D, Cullinane A. Equine influenza: Antigenic drift and implications for vaccines. Equine Vet. J. 2013;45:768–769.
    doi: 10.1111/evj.12148pubmed: 24117935google scholar: lookup
  16. . OIE Expert Surveillance Panel on Equine Influenza Vaccine Composition—Videoconference, 16 April 2020. Bull. Off. 2020;2020:1–5.
    doi: 10.20506/bull.2020.1.3134google scholar: lookup
  17. Spackman E, Senne DA, Myers TJ, Bulaga LL, Garber LP, Perdue ML, Lohman K, Daum LT, Suarez DL. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol. 2002;40:3256–3260.
    doi: 10.1128/JCM.40.9.3256-3260.2002pmc: PMC130722pubmed: 12202562google scholar: lookup
  18. Heine HG, Foord AJ, Wang J, Valdeter S, Walker S, Morrissy C, Wong FYK, Meehan B. Detection of highly pathogenic zoonotic influenza virus H5N6 by reverse-transcriptase quantitative polymerase chain reaction. Virol. J. 2015;12:10–13.
    doi: 10.1186/s12985-015-0250-3pmc: PMC4328077pubmed: 25889293google scholar: lookup
  19. Laconi A, Fortin A, Bedendo G, Shibata A, Sakoda Y, Awuni JA, Go-Maro E, Arafa A, Maken Ali AS, Terregino C. Detection of avian influenza virus: A comparative study of the in silico and in vitro performances of current RT-qPCR assays. Sci. Rep. 2020;10:8441.
    doi: 10.1038/s41598-020-64003-6pmc: PMC7242438pubmed: 32439885google scholar: lookup
  20. . Equine Influenza (Infection with Equine Influenza Virus). Elsevier; Amsterdam, The Netherlands: 2019. Chapter 3.6.7.
  21. Woodward AL, Rash AS, Blinman D, Bowman S, Chambers TM, Daly JM, Damiani A, Joseph S, Lewis N, McCauley JW. Development of a surveillance scheme for equine influenza in the UK and characterisation of viruses isolated in Europe, Dubai and the USA from 2010–2012. Vet. Microbiol. 2014;169:113–127.
    doi: 10.1016/j.vetmic.2013.11.039pubmed: 24480583google scholar: lookup
  22. Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP. Mrbayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 2012;61:539–542.
    doi: 10.1093/sysbio/sys029pmc: PMC3329765pubmed: 22357727google scholar: lookup
  23. Huelsenbeck JP, Larget B, Alfaro ME. Bayesian phylogenetic model selection using reversible jump Markov chain Monte Carlo. Mol. Biol. Evol. 2004;21:1123–1133.
    doi: 10.1093/molbev/msh123pubmed: 15034130google scholar: lookup
  24. R Development Core Team. A Language and Environment for Statistical Computing. Volume 1. R Foundation for Statistical Computing; Vienna, Austria: 2020.
  25. Yu G. Using ggtree to Visualize Data on Tree-Like Structures. Curr. Protoc. Bioinform. 2020;69:e96.
    doi: 10.1002/cpbi.96pubmed: 32162851google scholar: lookup
  26. Wickham H, Averick M, Bryan J, Chang W, McGowan L, François R, Grolemund G, Hayes A, Henry L, Hester J. Welcome to the Tidyverse. J. Open Source Softw. 2019;4:1686.
    doi: 10.21105/joss.01686google scholar: lookup
  27. Cullinane A, Gahan J, Walsh C, Nemoto M, Entenfellner J, Olguin-Perglione C, Garvey M, Fu TQH, Venner M, Yamanaka T. Evaluation of current equine influenza vaccination protocols prior to shipment, guided by OIE standards. Vaccines 2020;8:107.
    doi: 10.3390/vaccines8010107pmc: PMC7157717pubmed: 32121419google scholar: lookup
  28. Fougerolle S, Fortier C, Legrand L, Jourdan M, Marcillaud-Pitel C, Pronost S, Paillot R. Success and limitation of equine influenza vaccination: The first incursion in a decade of a florida clade 1 equine influenza virus that shakes protection despite high vaccine coverage. Vaccines 2019;7:174.
    doi: 10.3390/vaccines7040174pmc: PMC6963532pubmed: 31684097google scholar: lookup
  29. Fougerolle S, Legrand L, Lecouturier F, Sailleau C, Paillot R, Hans A, Pronost S. Genetic evolution of equine influenza virus strains (H3N8) isolated in France from 1967 to 2015 and the implications of several potential pathogenic factors. Virology 2017;505:210–217.
    doi: 10.1016/j.virol.2017.02.003pubmed: 28292661google scholar: lookup
  30. Both GW, Sleigh MJ, Cox NJ, Kendal AP. Antigenic drift in influenza virus H3 hemagglutinin from 1968 to 1980: Multiple evolutionary pathways and sequential amino acid changes at key antigenic sites. J. Virol. 1983;48:52–60.
    doi: 10.1128/jvi.48.1.52-60.1983pmc: PMC255321pubmed: 6193288google scholar: lookup
  31. Whitlock F, Grewar J, Newton R. An epidemiological overview of the equine influenza epidemic in Great Britain during 2019. Equine Vet. J. 2022;55:153–164.
    doi: 10.1111/evj.13874pmc: PMC10087154pubmed: 36054725google scholar: lookup
  32. . OIE Expert surveillance panel on equine influenza vaccine composition. Woah 2022;2022:1–3.
  33. Chambers TM. A brief introduction to equine influenza and equine influenza viruses. Methods Mol. Biol. 2020;2123:355–360.
    doi: 10.1007/978-1-0716-0346-8_26pubmed: 32170701google scholar: lookup
  34. . European Commission Regulation (EU) 2016/429 of the European Parliament and of the Council of 9 March 2016 on transmissible animal diseases and amending and repealing certain acts in the area of animal health (“Animal Health Law”). Off. J. Eur. Union. 2016;84:1–208.
  35. . Decreto del Presidente della Republica 8 febbraio 1954, n. 320. Regolamento di polizia veterinaria. 1954, 320.

Citations

This article has been cited 1 times.
  1. Branda F, Yon DK, Albanese M, Binetti E, Giovanetti M, Ciccozzi A, Ciccozzi M, Scarpa F, Ceccarelli G. Equine Influenza: Epidemiology, Pathogenesis, and Strategies for Prevention and Control. Viruses 2025 Feb 21;17(3).
    doi: 10.3390/v17030302pubmed: 40143233google scholar: lookup
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