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Cytogenetic and genome research2004; 102(1-4); 222-225; doi: 10.1159/000075753

FISH analysis comparing genome organization in the domestic horse (Equus caballus) to that of the Mongolian wild horse (E. przewalskii).

Abstract: Przewalski's wild horse (E. przewalskii, EPR) has a diploid chromosome number of 2n = 66 while the domestic horse (E. caballus, ECA) has a diploid chromosome number of 2n = 64. Discussions about their phylogenetic relationship and taxonomic classification have hinged on comparisons of their skeletal morphology, protein and mitochondrial DNA similarities, their ability to produce fertile hybrid offspring, and on comparison of their chromosome morphology and banding patterns. Previous studies of GTG-banded karyotypes suggested that the chromosomes of both equids were homologous and the difference in chromosome number was due to a Robertsonian event involving two pairs of acrocentric chromosomes in EPR and one pair of metacentric chromosomes in ECA (ECA5). To determine which EPR chromosomes were homologous to ECA5 and to confirm the predicted chromosome homologies based on GTG banding, we constructed a comparative gene map between ECA and EPR by FISH mapping 46 domestic horse-derived BAC clones containing genes previously mapped to ECA chromosomes. The results indicated that all ECA and EPR chromosomes were homologous as predicted by GTG banding, but provide new information in that the EPR acrocentric chromosomes EPR23 and EPR24 were shown to be homologues of the ECA metacentric chromosome ECA5.
Copyright 2003 S. Karger AG, Basel
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Publication Date: 2004-02-19 PubMed ID: 14970707DOI: 10.1159/000075753Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores the comparison of the genome organization between the domestic horse and the Mongolian wild horse using Fluorescence in situ Hybridization (FISH) analysis. The study was conducted to confirm the homology between the chromosomes of both horse species and identified specific chromosomes that are homologous.

Objective of the research

  • The research aims to compare the genome organization between the domestic horse (Equus caballus) and the Mongolian wild horse (E. przewalskii) using Fluorescence in situ Hybridization (FISH) analysis.
  • It discusses the phylogenetic relationship and taxonomic classification of the two species based on their chromosome morphology, banding patterns, and genome mapping.
  • The study is conducted to confirm the previously predicted homology between the chromosomes of both horse species and to determine the specific chromosomes that are homologous.

Methodology of the research

  • The research employed FISH mapping, a technique used in cytogenetics that uses fluorescent probes to bind parts of the chromosome having its DNA sequence.
  • The study used 46 domestic horse-derived BAC clones containing genes previously mapped to domestic horse chromosomes.
  • The homologies between the chromosomes were confirmed based on GTG banding, a technique for staining the chromosomes to reveal its characteristic pattern.

Findings of the research

  • The analysis indicated that all chromosomes of both horse species were homologous as predicted by GTG banding. This means that the chromosome pairings in the two species are evolutionarily related and share a common ancestor.
  • The analytical findings provide new information that the acrocentric chromosomes, EPR23 and EPR24, in the Mongolian wild horse are shown to be homologues of the metacentric chromosome, ECA5, in the domestic horse.

Implications of the research

  • The confirmation and identification of the homology between the chromosomes of both horse species can contribute significant knowledge in the fields of genetics and evolutionary biology.
  • This can help in understanding the genetic variation across species and can be useful in studying the evolution, genetic diseases, and breeding of horses.
  • Further studies can be conducted based on the findings of this research for a better understanding of the genetic relationship between different species.

Cite This Article

APA
Myka JL, Lear TL, Houck ML, Ryder OA, Bailey E. (2004). FISH analysis comparing genome organization in the domestic horse (Equus caballus) to that of the Mongolian wild horse (E. przewalskii). Cytogenet Genome Res, 102(1-4), 222-225. https://doi.org/10.1159/000075753

Publication

Cytogenetic and genome research
ISSN: 1424-859X
NlmUniqueID: 101142708
Country: Switzerland
Language: English
Volume: 102
Issue: 1-4
Pages: 222-225

Researcher Affiliations

Myka, J L
  • M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546-0099, USA.
Lear, T L
    Houck, M L
      Ryder, O A
        Bailey, E

          MeSH Terms

          • Animals
          • Animals, Domestic / genetics
          • Animals, Wild / genetics
          • Cell Line
          • Chromosome Banding / methods
          • Chromosome Banding / veterinary
          • Chromosome Mapping / methods
          • Chromosome Mapping / veterinary
          • DNA Probes / genetics
          • Fibroblasts / chemistry
          • Fibroblasts / cytology
          • Fibroblasts / metabolism
          • Genome
          • Horses / genetics
          • In Situ Hybridization, Fluorescence / methods
          • In Situ Hybridization, Fluorescence / veterinary
          • Mongolia
          • Sequence Homology, Nucleic Acid

          Citations

          This article has been cited 12 times.
          1. Ali M. Tibetan wild ass, Equus kiang, in the literature: a comprehensive review. J Equine Sci 2025;36(4):115-127.
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