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Home / Equine Research Bank / Sci Rep / Fluticasone/salmeterol reduces remodelling and neutrophilic ...
Scientific reports2017; 7(1); 8843; doi: 10.1038/s41598-017-09414-8

Fluticasone/salmeterol reduces remodelling and neutrophilic inflammation in severe equine asthma.

Abstract: Asthmatic airways are inflamed and undergo remodelling. Inhaled corticosteroids and long-acting β2-agonist combinations are more effective than inhaled corticosteroid monotherapy in controlling disease exacerbations, but their effect on airway remodelling and inflammation remains ill-defined. This study evaluates the contribution of inhaled fluticasone and salmeterol, alone or combined, to the reversal of bronchial remodelling and inflammation. Severely asthmatic horses (6 horses/group) were treated with fluticasone, salmeterol, fluticasone/salmeterol, or with antigen avoidance for 12 weeks. Lung function, central and peripheral airway remodelling, and bronchoalveolar inflammation were assessed. Fluticasone/salmeterol and fluticasone monotherapy decreased peripheral airway smooth muscle remodelling after 12 weeks (p = 0.007 and p = 0.02, respectively). On average, a 30% decrease was observed with both treatments. In central airways, fluticasone/salmeterol reversed extracellular matrix remodelling after 12 weeks, both within the lamina propria (decreased thickness, p = 0.005) and within the smooth muscle layer (p = 0.004). Only fluticasone/salmeterol decreased bronchoalveolar neutrophilia (p = 0.03) to the same extent as antigen avoidance already after 8 weeks. In conclusion, this study shows that fluticasone/salmeterol combination decreases extracellular matrix remodelling in central airways and intraluminal neutrophilia. Fluticasone/salmeterol and fluticasone monotherapy equally reverse peripheral airway smooth muscle remodelling.
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Publication Date: 2017-08-18 PubMed ID: 28821845PubMed Central: PMC5562887DOI: 10.1038/s41598-017-09414-8Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study aimed to evaluate the impacts of fluticasone and salmeterol, alone and in combination, in reducing bronchial inflammation and remodelling in severely asthmatic horses, and observed that a combination of both drugs effectively decreased inflammation and reversed airway damage.

Methodology

  • The study involved severely asthmatic horses, categorized in groups of six. The groups were treated with either fluticasone, salmeterol, a combination of the two, or had their exposure to allergens limited for a duration of 12 weeks.
  • The researchers assessed lung function, alterations in both central and peripheral airways (remodelling), and inflammation in the bronchoalveolar areas. Remodelling refers to structural changes that occur in the respiratory airways due to asthma.

Results

  • The use of both fluticasone/salmeterol and fluticasone by itself resulted in a decrease in remodelling of the smooth muscle in peripheral airways after 12 weeks of treatment, demonstrating that both treatments were equally effective in reversing these damages.
  • In terms of the central airways, only the combined treatment of fluticasone/salmeterol notably reversed the remodelling of extracellular matrix within the lamina propria (layers of loose connective tissue) and the smooth muscle layer after 12 weeks.
  • In terms of inflammation, specifically bronchoalveolar neutrophilia (an increase in neutrophils which are types of white blood cells that fight inflammation), only the fluticasone/salmeterol combination was able to decrease it to levels comparable to reduction achieved by antigen avoidance, and that too within a shorter period (8 weeks).

Conclusion

  • The study concluded that a combination of fluticasone and salmeterol is effective in decreasing inflammation (neutrophilia) in bronchoalveolar regions, as well as in reversing extracellular matrix remodelling in central airways.
  • The same combination, as well as fluticasone monotherapy, were also found to equally reverse remodelling in peripheral airway smooth muscles.

Cite This Article

APA
Bullone M, Vargas A, Elce Y, Martin JG, Lavoie JP. (2017). Fluticasone/salmeterol reduces remodelling and neutrophilic inflammation in severe equine asthma. Sci Rep, 7(1), 8843. https://doi.org/10.1038/s41598-017-09414-8

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 7
Issue: 1
Pages: 8843
PII: 8843

Researcher Affiliations

Bullone, Michela
  • Université de Montréal, Faculty of Veterinary Medicine, Department of Clinical Sciences, 3200 rue Sicotte, St-Hyacinthe, J2S 2M2, Q, Canada.
Vargas, Amandine
  • Université de Montréal, Faculty of Veterinary Medicine, Department of Clinical Sciences, 3200 rue Sicotte, St-Hyacinthe, J2S 2M2, Q, Canada.
Elce, Yvonne
  • Université de Montréal, Faculty of Veterinary Medicine, Department of Clinical Sciences, 3200 rue Sicotte, St-Hyacinthe, J2S 2M2, Q, Canada.
  • University of Edinburgh, Royal (Dick) School of Veterinary Studies, Easter Bush Campus, EH25 9RG, United Kingdom.
Martin, James G
  • McGill University, Meakins Christie Laboratories, McGill University Health Center Research Institute, 1001 Decarie Blvd, Montreal, H4A 3J1, Q, Canada.
Lavoie, Jean-Pierre
  • Université de Montréal, Faculty of Veterinary Medicine, Department of Clinical Sciences, 3200 rue Sicotte, St-Hyacinthe, J2S 2M2, Q, Canada. jean-pierre.lavoie@umontreal.ca.

MeSH Terms

  • Adolescent
  • Airway Remodeling / drug effects
  • Animals
  • Antigens / immunology
  • Asthma / diagnosis
  • Asthma / drug therapy
  • Asthma / etiology
  • Bronchodilator Agents / pharmacology
  • Child
  • Disease Progression
  • Extracellular Matrix / metabolism
  • Female
  • Fluticasone / pharmacology
  • Horses
  • Humans
  • Male
  • Muscle, Smooth / drug effects
  • Muscle, Smooth / metabolism
  • Neutrophil Infiltration / drug effects
  • Neutrophil Infiltration / immunology
  • Neutrophils / drug effects
  • Neutrophils / immunology
  • Neutrophils / metabolism
  • Salmeterol Xinafoate / pharmacology
  • Severity of Illness Index
  • Young Adult

Grant Funding

  • MOP-102751 / CIHR

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 21 times.
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