Follicular fluid lipoproteins in the mare: evaluation of HDL transfer from plasma to follicular fluid.
Abstract: Using a density gradient ultracentrifugal procedure, we have separated equine plasma and follicular fluid high-density lipoproteins (HDL). The density distribution of the follicular fluid HDL was clearly displaced towards the highest densities in comparison with that of plasma HDL. Similarly, an analysis of size distributions showed a decrease in follicular fluid HDL diameters (4.2 to 9.2 nm) compared to plasma HDL (5.5 to 9.5 nm). HDL were isolated into three subfractions on the basis of the disposition of the Sudan Black stained bands in the centrifuge tubes. Concentrations of each subfraction were clearly lower in the follicular fluid, and the relative percentages with regard to the plasma equivalents were inversely proportional to the molecular weights (23.8% for HDL-1, 49.9% for HDL-2 and 63.7% for HDL-3). The cholesterol/phospholipid molar ratio and the esterified/free cholesterol molar ratio were clearly increased in the follicular HDL-2 and HDL-3 subfractions. The apolipoprotein distribution in follicular fluid HDL was very close to that in plasma HDL. LCAT activity measured in human as well as equine samples was weaker in follicular fluid compared to plasma in both species (4.0 nmol of free cholesterol esterified per h per ml vs. 24 nmol per h per ml). Theoretical concentrations of follicular fluid HDL were calculated assuming that the HDL particles would be merely a filtration product undergoing no detectable metabolic modifications. Biochemical measurements showed that the lightest particles (HDL-1) were less numerous than suggested by the theoretical calculation. Thus, although follicular fluid HDL appear to be a filtration product of plasma HDL, they undergo metabolic transformations that we suggest may be linked to hormonal synthesis and reverse cholesterol transport.
Publication Date: 1994-01-03 PubMed ID: 8280774DOI: 10.1016/0005-2760(94)90125-2Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This research paper involves studying the high-density lipoprotein (HDL) present in the follicular fluid within mares, specifically focusing on the transfer of these lipoproteins from plasma. The study reported that the HDL in the follicular fluid had distinct differences in density and size compared to the plasma HDL, and it contains different subfractions that displayed varying concentrations and molecular weights. The HDL appeared to undergo metabolic changes, possibly associated with hormonal synthesis and reverse cholesterol transport.
Follicular Fluid and High-Density Lipoprotein (HDL)
- This research focused on understanding the composition of high-density lipoprotein (HDL) in the follicular fluid of mares.
- The researchers used a process known as density gradient ultracentrifugal procedure to separate HDL from the plasma and follicular fluid.
- What they found was that the HDL in the follicular fluid had a higher density as compared to the plasma HDL.
Size and Subfractions of HDL
- The researchers also compared size distributions and found that the follicular fluid HDL diameters were smaller than plasma HDL diameters.
- They divided the HDL into three subfractions based on their locations in the centrifuge tubes, with each subfraction having lower concentrations in the follicular fluid compared to the plasma.
- The ratios of cholesterol to phospholipids and esterified cholesterol to free cholesterol were higher in the follicular fluid HDL-2 and HDL-3 subfractions.
Apolipoprotein Distribution and LCAT Activity
- The distribution of apolipoprotein, protein forms that bind lipids to form lipoprotein particles, in follicular fluid HDL was found to be similar to plasma HDL.
- The activity of Lecithin-Cholesterol Acyltransferase (LCAT), an enzyme important in lipoprotein metabolism, was detected to be lower in follicular fluid than in the plasma.
Hypotheses for Metabolic Transformations
- The researchers formulated a theory that HDL in follicular fluid were being filtered from the plasma HDL, but also underwent metabolic modifications.
- Specifically, they found that the lightest particles (HDL-1) were less numerous than what the theoretical calculations based on filtration suggested.
- They propose these metabolic changes might be related to hormone synthesis and the reverse transport of cholesterol.
Cite This Article
APA
Le Goff D.
(1994).
Follicular fluid lipoproteins in the mare: evaluation of HDL transfer from plasma to follicular fluid.
Biochim Biophys Acta, 1210(2), 226-232.
https://doi.org/10.1016/0005-2760(94)90125-2 Publication
Researcher Affiliations
- Laboratoire de Biochimie, URA CNRS 609, Université de Caen, France.
MeSH Terms
- Animals
- Apolipoproteins / analysis
- Centrifugation, Density Gradient
- Female
- Follicular Fluid / chemistry
- Follicular Fluid / metabolism
- Horses
- Humans
- Lipoproteins / chemistry
- Lipoproteins / metabolism
- Lipoproteins / ultrastructure
- Lipoproteins, HDL / blood
- Lipoproteins, HDL / metabolism
- Phosphatidylcholine-Sterol O-Acyltransferase / analysis
Citations
This article has been cited 10 times.- Arias A, Quiroz A, Santander N, Morselli E, Busso D. Implications of High-Density Cholesterol Metabolism for Oocyte Biology and Female Fertility. Front Cell Dev Biol 2022;10:941539.
- Kim K, Bloom MS, Browne RW, Bell EM, Yucel RM, Fujimoto VY. Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients. J Assist Reprod Genet 2017 Jan;34(1):1-10.
- Kim K, Bloom MS, Fujimoto VY, Bell EM, Yucel RM, Browne RW. Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles. J Assist Reprod Genet 2016 Mar;33(3):423-430.
- Yesilaltay A, Dokshin GA, Busso D, Wang L, Galiani D, Chavarria T, Vasile E, Quilaqueo L, Orellana JA, Walzer D, Shalgi R, Dekel N, Albertini DF, Rigotti A, Page DC, Krieger M. Excess cholesterol induces mouse egg activation and may cause female infertility. Proc Natl Acad Sci U S A 2014 Nov 18;111(46):E4972-80.
- DeAngelis AM, Roy-O'Reilly M, Rodriguez A. Genetic alterations affecting cholesterol metabolism and human fertility. Biol Reprod 2014 Nov;91(5):117.
- Bloom MS, Kim K, Fujimoto VY, Browne RW. Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis. Fertil Steril 2014 May;101(5):1431-40.
- Gautier T, Becker S, Drouineaud V, Ménétrier F, Sagot P, Nofer JR, von Otte S, Lagrost L, Masson D, Tietge UJ. Human luteinized granulosa cells secrete apoB100-containing lipoproteins. J Lipid Res 2010 Aug;51(8):2245-52.
- Miettinen HE, Rayburn H, Krieger M. Abnormal lipoprotein metabolism and reversible female infertility in HDL receptor (SR-BI)-deficient mice. J Clin Invest 2001 Dec;108(11):1717-22.
- Travert C, Fofana M, Carreau S, Le Goff D. Rat Leydig cells use apolipoprotein E depleted high density lipoprotein to regulate testosterone production. Mol Cell Biochem 2000 Oct;213(1-2):51-9.
- Bresnahan DR, Catandi GD, Peters SO, Maclellan LJ, Broeckling CD, Carnevale EM. Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares. Front Cell Dev Biol 2023;11:1280998.
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