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Molecular reproduction and development2022; 89(7); 298-311; doi: 10.1002/mrd.23622

Follicular-fluid proteomics during equine follicle development.

Abstract: The complex composition of the follicular fluid (FF), the intimate proximity to the oocyte, and the continual changes in their composition have a major effect on folliculogenesis and oogenesis. To date, the profiling of FF proteomes during follicle selection, development, and ovulation has not been comprehensively investigated. Therefore, a shotgun proteomics approach and bioinformatics analyses were used to profile the proteomes of equine FF harvested in vivo from follicles at the following development stages: predeviation (18-20 mm), deviation (22-25 mm), postdeviation (26-29 mm), preovulatory (30-35 mm), and impending ovulation. A total of 294 proteins were detected in FF (FDR <1%), corresponding to 65 common proteins and 124, 142, 167, 132, and 142 proteins in the predeviation, deviation, postdeviation, preovulatory, and impending ovulation groups, respectively. The higher expression of properdin and several other proteins belonging to the complement system during the deviation time and ovulation suggested their contribution in the selection of the future dominant follicle and ovulation. Apolipoprotein A-1 and antithrombin-III appeared to be important throughout folliculogenesis. The "complement and coagulation cascades" was the major KEGG pathway across all stages of follicle development. The significant expression of several proteins belonging to the serine-type endopeptidase indicated their likely contribution to follicle and oocyte development. Our data provide an extensive description and functional analyses of the equine FF proteome during follicle selection, development, and ovulation. This information will help improve understanding of the ovarian function and ovulatory dysfunctions and might serve as a reference for future biomarker discovery for oocyte quality assessment.
© 2022 Wiley Periodicals LLC.
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Publication Date: 2022-06-27 PubMed ID: 35762042DOI: 10.1002/mrd.23622Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • N.I.H.
  • Extramural

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the different protein compositions in the follicular fluid (FF) of horses during various stages of follicular development, providing an understanding of their effects on the process of folliculogenesis and oogenesis. The study utilized a shotgun proteomics approach and data processing tools, allowing a thorough understanding of the proteins’ roles in these processes and deriving potential biomarkers for assessing oocyte quality.

Study Design and Methods

  • The researchers used a method known as ‘shotgun proteomics’ and various bioinformatics tools to profile the proteins present in equine FF. Shotgun proteomics is a large-scale analysis technique that allows the identification and quantification of proteins in a complex biological sample.
  • Equine FF was collected in vivo (in the live animal) from follicles at different stages of development including predeviation, deviation, postdeviation, preovulatory, and impending ovulation.

Findings and Observations

  • A total of 294 proteins were identified across all stages of follicular development. These proteins were categorized into 65 common proteins and specific proteins for each stage of development.
  • Properdin and other proteins belonging to the complement system showed increased expression during the deviation stage and ovulation, indicating their role in follicle selection and ovulation.
  • Apolipoprotein A-1 and antithrombin-III appear to play critical roles throughout folliculogenesis.
  • The major KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway identified across all stages was the “complement and coagulation cascades”. This suggests these pathways are crucial for follicle development.
  • Higher expression of proteins belonging to the serine-type endopeptidase family suggests their likely involvement in follicle and oocyte development.

Implications and Conclusions

  • The findings offer comprehensive insights into the protein composition of equine FF during various stages of follicle development and ovulation.
  • Understanding these protein profiles can enhance knowledge of ovarian function and ovulatory dysfunctions.
  • This study holds significant potential for future clinical applications, serving as a foundation for biomarker discovery to assess oocyte quality. This could be valuable for fertility studies and treatments in horses and potentially other types of livestock or even human fertility.

Cite This Article

APA
Ishak GM, Feugang JM, Pechanova O, Pechan T, Peterson DG, Willard ST, Ryan PL, Gastal EL. (2022). Follicular-fluid proteomics during equine follicle development. Mol Reprod Dev, 89(7), 298-311. https://doi.org/10.1002/mrd.23622

Publication

Molecular reproduction and development
ISSN: 1098-2795
NlmUniqueID: 8903333
Country: United States
Language: English
Volume: 89
Issue: 7
Pages: 298-311

Researcher Affiliations

Ishak, Ghassan M
  • Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
  • Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, Illinois, USA.
Feugang, Jean M
  • Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, Mississippi, USA.
Pechanova, Olga
  • Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, Mississippi, USA.
Pechan, Tibor
  • Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, Mississippi, USA.
Peterson, Daniel G
  • Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, Mississippi, USA.
Willard, Scott T
  • Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, Mississippi, USA.
Ryan, Peter L
  • Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, Mississippi, USA.
Gastal, Eduardo L
  • Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, Illinois, USA.

MeSH Terms

  • Animals
  • Female
  • Follicular Fluid / metabolism
  • Horses
  • Ovarian Follicle / metabolism
  • Ovulation
  • Proteome / metabolism
  • Proteomics

Grant Funding

  • P20 GM103476 / NIGMS NIH HHS

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Citations

This article has been cited 7 times.
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