Fourier transform infrared microspectroscopic investigation of the organic and mineral constituents of peritubular dentin: a horse study.
Abstract: Peritubular dentin (PTD) is a relatively dense mineralized tissue surrounding tooth dentin tubules, whose composition and mode of formation are still unclear. Fourier transform infrared microspectroscopic studies of the organic and mineral components of the highly developed horse PTD indicate that the peritubular matrix is less abundant than the intertubular matrix but is also mainly composed of collagen, which is more hydrated. These data suggest that most of the crystals are located outside the collagen fibrils and probably not associated with protein components. The crystals in PTD have nearly the same crystallinity as those in intertubular spaces, showing comparable amounts of carbonate ions, although some PO4 groups have different nonapatitic environments. Horse PTD composition is very similar to that of ITD, with collagen as the main protein component and carbonated apatite as the mineral fraction but the different proportions of these constituents and the greater water content in PTD suggest a different organization.
Publication Date: 2002-07-23 PubMed ID: 12200650DOI: 10.1007/s00223-001-2108-5Google Scholar: Lookup
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- Journal Article
Summary
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The study focuses on the investigation of the mineral and organic constituents of peritubular dentin (PTD) in horses using Fourier transform infrared microspectroscopy. The findings revealed that the peritubular matrix has less abundant but more hydrated collagen content compared to the intertubular matrix, and that the crystals in PTD aren’t likely associated with protein components.
Research Methodology
- Fourier transform infrared microspectroscopy, a powerful analytical tool for examining the organic and mineral components of a substance, was used in this study.
- This method provides detailed and reliable data about the biochemical composition of tissues, as well as their morphological and structural characteristics.
- The study focused on PTD in horses, known for their highly developed dentine structures.
Major Findings
- The study revealed that the peritubular matrix is less abundant than the intertubular matrix.
- The peritubular matrix is primarily composed of collagen which is more hydrated compared to the one in the intertubular matrix.
- Most of the crystals are found outside the collagen fibrils and are probably not associated with protein components.
- The crystals within the PTD exhibited almost the same crystallinity as those present in intertubular spaces.
- Both the PTD and the crystals in intertubular spaces showed comparable levels of carbonate ions. However, some PO4 groups have different nonapatitic environments.
Implications and Conclusions
- There is a striking similarity between the composition of horse PTD and ITD. Both are predominantly composed of collagen – the main protein component and carbonated apatite – the mineral fraction.
- However, the different proportions of these constituents in both PTD and ITD point towards a possible difference in organization.
- There is a greater water content within PTD, which may be significant for studying the hydration properties and strengths of different tissue types. It indirectly influences the hardness, elasticity, and fracture-resistance properties of the tissue.
Cite This Article
APA
Magne D, Guicheux J, Weiss P, Pilet P, Daculsi G.
(2002).
Fourier transform infrared microspectroscopic investigation of the organic and mineral constituents of peritubular dentin: a horse study.
Calcif Tissue Int, 71(2), 179-185.
https://doi.org/10.1007/s00223-001-2108-5 Publication
Researcher Affiliations
- INSERM EMI 99-03, Research Center on Materials of Biological Interests, Nantes, France. david.magne@sante.univ-nantes.fr
MeSH Terms
- Animals
- Collagen / analysis
- Collagen / ultrastructure
- Dentin / chemistry
- Dentin / ultrastructure
- Horses
- Microscopy, Electron, Scanning
- Minerals / analysis
- Spectroscopy, Fourier Transform Infrared / methods
- Spectroscopy, Fourier Transform Infrared / veterinary
- Tooth / chemistry
- Tooth / ultrastructure
Citations
This article has been cited 5 times.- Iijima M, Ishiyama M. A unique mineralization mode of hypermineralized pleromin in the tooth plate of Chimaera phantasma contributes to its microhardness.. Sci Rep 2020 Oct 29;10(1):18591.
- Huang L, Zhang X, Shao J, Zhou Z, Chen Y, Hu X. Nanoscale chemical and mechanical heterogeneity of human dentin characterized by AFM-IR and bimodal AFM.. J Adv Res 2020 Mar;22:163-171.
- Xu C, Wang Y. Chemical composition and structure of peritubular and intertubular human dentine revisited.. Arch Oral Biol 2012 Apr;57(4):383-91.
- Sato S, Tsuchiya M, Komaki K, Kusunoki S, Tsuchiya S, Haruyama N, Takahashi I, Sasano Y, Watanabe M. Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts.. Histochem Cell Biol 2009 May;131(5):583-91.
- Habelitz S, Rodriguez BJ, Marshall SJ, Marshall GW, Kalinin SV, Gruverman A. Peritubular dentin lacks piezoelectricity.. J Dent Res 2007 Sep;86(9):908-11.
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