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Home / Equine Research Bank / Int J Vet Sci Med / Freeze-drying protocols and methods of maintaining the in-vi...
International journal of veterinary science and medicine2024; 12(1); 71-80; doi: 10.1080/23144599.2024.2380586

Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate.

Abstract: Platelet lysate, derived from platelets, are valuable biological products rich in bioactive molecules. Their use promotes tissue healing and modulates inflammation. However, maintaining the stability and bioactivity of platelet lysate is challenging since they degrade rapidly at room temperature. This study focused on the possibility to confer enhanced stability to freeze-dried equine platelet lysate as an alternative to platelet-rich plasma (PRP). Platelet lysate (PL) was derived from PRP and freeze-dried either as such or using various adjuvants. Primary cell cultures of porcine Vascular Wall-Mesenchymal Stem Cells were treated with different PL formulations, and cell viability was assessed using an MTT assay. Overall, the addition of PL significantly improved cell viability as compared to controls without growth factor supplementation or with foetal bovine serum. Notably, the freeze-drying process maintained the effectiveness of the PL for at least a week. Furthermore, the study revealed that varying the horse as the source of PL could yield varying effects on cell viability. Detailed freeze-drying protocols were established, including freezing, primary drying and secondary drying phases, and the type of adjuvant. This study demonstrated the potential of freeze-dried equine PL as a viable alternative to PRP and highlighted the importance of precise freeze-drying protocols and adjuvants for standardization. Equine PL showed promise for medical treatment in horses, offering advantages such as extended shelf life, ease of handling, and reduced transportation costs, with the potential for broadened therapeutic usage.
© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
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Publication Date: 2024-08-07 PubMed ID: 39119550PubMed Central: PMC11308971DOI: 10.1080/23144599.2024.2380586Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the possibilities of freeze-drying horse platelet lysate (PL), a biological product rich in healing and inflammation regulating elements, to enhance its bioactivity and stability. Freeze-dried equine PL was tested on primary cell cultures and compared to controls to assess cell viability, and freeze-drying protocols were detailed for further use and standardization.

About Platelet Lysate (PL)

  • PL is a biological product derived from platelets, cells in blood that help with clotting.
  • It is rich in bioactive molecules, which promote tissue healing and modulate inflammation.
  • PL needs to be kept stable to maintain its bioactivity, which is usually tricky as it rapidly degrades at room temperature.
  • The study offers freeze-drying as a solution for enhancing the stability and prolonging the bioactive nature of PL.

Freeze-drying Horse PL

  • In the study, PL derived from horse (equine) platelets was freeze-dried to increase its stability.
  • Different formulations of the freeze-dried PL were tested on primary cell cultures of porcine Vascular Wall-Mesenchymal Stem Cells to assess cell viability.
  • The use of PL significantly increased cell viability compared to controls without growth factor supplementation or with foetal bovine serum.

Importance of Detailed Protocols and Adjuvants

  • The study stresses the importance of having detailed freeze-drying protocols for standardization, comprising freezing, primary drying, and secondary drying phases.
  • It was found that using different adjuvants – substances that enhance the body’s immune response to an antigen, led to differing results.
  • Variations in the horses used as sources of PL also created differences in the effect on cell viability. This emphasizes the significance of standardized protocols and the careful selection of adjuvants and PL sources.

Benefit and Applications of Freeze-dried Horse Platelet Lysate

  • Freeze-dried equine PL could be a legitimate alternative to PRP (platelet-rich plasma). PRP is currently in extensive medical use, and freeze-drying could present a method to increase its shelf life and ease of handling.
  • It also holds potential for therapeutic uses beyond its current applications, due to its enhanced stability and viability.

Cite This Article

APA
Bernardini C, Romagnoli N, Casalini I, Turba ME, Spadari A, Forni M, Gentilini F. (2024). Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. Int J Vet Sci Med, 12(1), 71-80. https://doi.org/10.1080/23144599.2024.2380586

Publication

International journal of veterinary science and medicine
ISSN: 2314-4599
NlmUniqueID: 101626221
Country: United States
Language: English
Volume: 12
Issue: 1
Pages: 71-80

Researcher Affiliations

Bernardini, Chiara
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.
  • Health Sciences and Technologies-Interdepartmental Center for Industrial Research (CIRI-SDV), Alma Mater Studiorum-University of Bologna, Bologna, Italy.
Romagnoli, Noemi
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.
Casalini, Isabelle
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.
Turba, Maria Elena
  • Genefast srl, Forlì, Italy.
Spadari, Alessandro
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.
Forni, Monica
  • Health Sciences and Technologies-Interdepartmental Center for Industrial Research (CIRI-SDV), Alma Mater Studiorum-University of Bologna, Bologna, Italy.
  • Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy.
Gentilini, Fabio
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.

Conflict of Interest Statement

No potential conflict of interest was reported by the author(s).

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