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Applied and environmental microbiology2004; 70(4); 2503-2507; doi: 10.1128/AEM.70.4.2503-2507.2004

Frequency and distribution of tetracycline resistance genes in genetically diverse, nonselected, and nonclinical Escherichia coli strains isolated from diverse human and animal sources.

Abstract: Nonselected and natural populations of Escherichia coli from 12 animal sources and humans were examined for the presence and types of 14 tetracycline resistance determinants. Of 1,263 unique E. coli isolates from humans, pigs, chickens, turkeys, sheep, cows, goats, cats, dogs, horses, geese, ducks, and deer, 31% were highly resistant to tetracycline. More than 78, 47, and 41% of the E. coli isolates from pigs, chickens, and turkeys were resistant or highly resistant to tetracycline, respectively. Tetracycline MICs for 61, 29, and 29% of E. coli isolates from pig, chickens, and turkeys, respectively, were >/=233 micro g/ml. Muliplex PCR analyses indicated that 97% of these strains contained at least 1 of 14 tetracycline resistance genes [tetA, tetB, tetC, tetD, tetE, tetG, tetK, tetL, tetM, tetO, tetS, tetA(P), tetQ, and tetX] examined. While the most common genes found in these isolates were tetB (63%) and tetA (35%), tetC, tetD, and tetM were also found. E. coli isolates from pigs and chickens were the only strains to have tetM. To our knowledge, this represents the first report of tetM in E. coli.
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Publication Date: 2004-04-07 PubMed ID: 15066850PubMed Central: PMC383146DOI: 10.1128/AEM.70.4.2503-2507.2004Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the presence and types of tetracycline resistance genes in diverse, nonselected Escherichia coli (E. coli) strains from animals and humans. The researchers found a high occurrence of these resistance genes, especially in E. coli strains from pigs and poultry.

Sampling and Analysis

The researchers collected and examined 1,263 unique E. coli samples from twelve different animal sources and humans. These sources were broad and included chickens, cows, deer, dogs, ducks, geese, goats, horses, pigs, sheep, turkeys, and humans.

  • The study sought to identify and classify 14 specific tetracycline resistance determinants present in these diverse E. coli samples.
  • Using multiplex PCR analyses, researchers were able to identify the presence of these genes in 97% of strains tested.

Resistance to Tetracycline

The results of this study indicated a considerable degree of resistance among these E. coli samples to tetracycline, a common antibiotic.

  • 31% of the samples showed high resistance to tetracycline.
  • 78%, 47% and 41% of E. coli isolates from pigs, chickens, and turkeys, respectively, showed either resistance or high resistance.
  • The levels of resistance were determined through measuring the Minimum Inhibitory Concentration (MIC) of tetracycline for each sample. The researchers found that the MICs for 61%, 29%, and 29% of E. coli isolates from pigs, chickens, and turkeys, respectively, reached or exceeded 233 micrograms per milliliter.

Distribution of Resistance Genes

The study found a variety of tetracycline-resistant genes in the E. coli samples.

  • The most prevalent genes were tetB (found in 63% of samples) and tetA (found in 35% of samples).
  • However, other genes such as tetC, tetD, and tetM were also identified.
  • Notably, the study discovered the presence of the tetM gene in E. coli samples from pigs and chickens. This marked the first report of its kind highlighting the existence of the tetM gene in E. coli.

Significance of the Study

The findings of this study are significant because they show the extensive distribution of tetracycline resistance genes in a variety of E. coli strains from diverse sources. This poses a potential threat to the effective use of tetracycline in treating bacterial infections in both animals and humans. Understanding the genetics of antibiotic resistance can help in the development of strategies to combat this growing health concern.

Cite This Article

APA
Bryan A, Shapir N, Sadowsky MJ. (2004). Frequency and distribution of tetracycline resistance genes in genetically diverse, nonselected, and nonclinical Escherichia coli strains isolated from diverse human and animal sources. Appl Environ Microbiol, 70(4), 2503-2507. https://doi.org/10.1128/AEM.70.4.2503-2507.2004

Publication

Applied and environmental microbiology
ISSN: 0099-2240
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 70
Issue: 4
Pages: 2503-2507

Researcher Affiliations

Bryan, Andrew
  • Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota 55108, USA.
Shapir, Nir
    Sadowsky, Michael J

      MeSH Terms

      • Animals
      • DNA, Bacterial / genetics
      • Escherichia coli / drug effects
      • Escherichia coli / genetics
      • Escherichia coli / isolation & purification
      • Genes, Bacterial
      • Humans
      • Molecular Epidemiology
      • Polymerase Chain Reaction
      • Species Specificity
      • Tetracycline Resistance / genetics

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