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Home / Equine Research Bank / J Vet Med Sci / Full genome sequences of zebra-borne equine herpesvirus type...
The Journal of veterinary medical science2014; 76(9); 1309-1312; doi: 10.1292/jvms.14-0183

Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson’s gazelle.

Abstract: A strain of equine herpesvirus type 1 (EHV-1) was isolated from zebra. This strain, called "zebra-borne EHV-1", was also isolated from an onager and a gazelle in zoological gardens in U.S.A. The full genome sequences of the 3 strains were determined. They shared 99% identities with each other, while they shared 98% and 95% identities with the horse derived EHV-1 and equine herpesvirus type 9, respectively. Sequence data indicated that the EHV-1 isolated from a polar bear in Germany is one of the zebra-borne EHV-1 and not a recombinant virus. These results indicated that zebra-borne EHV-1 is a subtype of EHV-1.
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Publication Date: 2014-06-11 PubMed ID: 24920546PubMed Central: PMC4197165DOI: 10.1292/jvms.14-0183Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study deals with the genome sequencing of a strain of equine herpesvirus type 1 (EHV-1) found in various animals including zebras, onagers, and gazelles. The researchers concluded that this strain, which they named “zebra-borne EHV-1”, is a subtype of EHV-1.

The Strain of EHV-1

  • The strain of equine herpesvirus type 1 (EHV-1) central to this study was originally derived from zebras. This is why it was named “zebra-borne EHV-1”.
  • Apart from the zebras, the same strain was also derived from an onager and a Thomson’s gazelle. These animals were all located in zoological gardens in the USA.

Determining the Full Genome Sequences

  • The researchers carried out full genome sequencing of the three strains of the virus found in the zebra, the onager, and the gazelle.
  • It was found that the three strains shared 99% DNA identities with each other, signifying that they were virtually the same strain.

Comparison with Other EHV-1 Types

  • When the zebra-borne EHV-1 was compared with EHV-1 derived from horses, it was found that they shared 98% DNA identity.
  • Comparatively, the zebra-borne EHV-1 shared only 95% DNA identity with equine herpesvirus type 9.
  • The researchers therefore concluded that the zebra-borne EHV-1 is closer in genetic make-up to the horse-derived EHV-1 than to equine herpesvirus type 9.

Zebra-Borne EHV-1 in Polar Bears

  • Sequence data from the study also indicated that a strain of EHV-1 isolated from a polar bear in Germany is one of the zebra-borne EHV-1. This suggested the strain is not limited to equines.
  • The fact that this strain turned out to be of the zebra-borne type and not a recombinant virus shows the extent to which this strain can spread among different species of animals, beyond just horses and other equines.

Concluding Remarks

  • From the research findings, it was concluded that zebra-borne EHV-1 can be considered a subtype of EHV-1. This is based on the high degree of DNA identity it shared with the horse-derived EHV-1.
  • Greater knowledge and understanding of these different EHV-1 subtypes can help in the later diagnosis and treatment of animals infected with the virus.

Cite This Article

APA
Guo X, Izume S, Okada A, Ohya K, Kimura T, Fukushi H. (2014). Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson’s gazelle. J Vet Med Sci, 76(9), 1309-1312. https://doi.org/10.1292/jvms.14-0183

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 76
Issue: 9
Pages: 1309-1312

Researcher Affiliations

Guo, Xiaoqin
  • Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Izume, Satoko
    Okada, Ayaka
      Ohya, Kenji
        Kimura, Takashi
          Fukushi, Hideto

            MeSH Terms

            • Amino Acid Sequence
            • Animals
            • Animals, Zoo
            • Base Sequence
            • DNA, Viral / chemistry
            • DNA, Viral / genetics
            • Equidae / virology
            • Herpesviridae Infections / veterinary
            • Herpesvirus 1, Equid / genetics
            • Molecular Sequence Data
            • Phylogeny
            • Polymerase Chain Reaction / veterinary
            • Polymorphism, Single Nucleotide / genetics
            • Sequence Alignment
            • Sequence Analysis, DNA

            References

            This article includes 21 references
            1. Abdelgawad A, Azab W, Damiani AM, Baumgartner K, Will H, Osterrieder N, Greenwood AD. Zebra-borne equine herpesvirus type 1 (EHV-1) infection in non-African captive mammals.. Vet Microbiol 2014 Feb 21;169(1-2):102-6.
              doi: 10.1016/j.vetmic.2013.12.011pubmed: 24440374google scholar: lookup
            2. Blunden AS, Smith KC, Whitwell KE, Dunn KA. Systemic infection by equid herpesvirus-1 in a Grevy's zebra stallion (Equus grevyi) with particular reference to genital pathology.. J Comp Pathol 1998 Nov;119(4):485-93.
              doi: 10.1016/S0021-9975(98)80043-1pubmed: 9839210google scholar: lookup
            3. Côté O, Viel L, Bienzle D. Phylogenetic relationships among Perissodactyla: secretoglobin 1A1 gene duplication and triplication in the Equidae family.. Mol Phylogenet Evol 2013 Dec;69(3):430-6.
              doi: 10.1016/j.ympev.2013.08.012pubmed: 23988306google scholar: lookup
            4. Fukushi H, Tomita T, Taniguchi A, Ochiai Y, Kirisawa R, Matsumura T, Yanai T, Masegi T, Yamaguchi T, Hirai K. Gazelle herpesvirus 1: a new neurotropic herpesvirus immunologically related to equine herpesvirus 1.. Virology 1997 Jan 6;227(1):34-44.
              doi: 10.1006/viro.1996.8296pubmed: 9015181google scholar: lookup
            5. Fukushi H, Yamaguchi T, Yamada S. Complete genome sequence of equine herpesvirus type 9.. J Virol 2012 Dec;86(24):13822.
              doi: 10.1128/JVI.02607-12pmc: PMC3503125pubmed: 23166237google scholar: lookup
            6. Ghanem YM, Fukushi H, Ibrahim ES, Ohya K, Yamaguchi T, Kennedy M. Molecular phylogeny of equine herpesvirus 1 isolates from onager, zebra and Thomson's gazelle.. Arch Virol 2008;153(12):2297-302.
              doi: 10.1007/s00705-008-0247-ypubmed: 19002555google scholar: lookup
            7. Gordon D, Green P. Consed: a graphical editor for next-generation sequencing.. Bioinformatics 2013 Nov 15;29(22):2936-7.
              doi: 10.1093/bioinformatics/btt515pmc: PMC3810858pubmed: 23995391google scholar: lookup
            8. Greenwood AD, Tsangaras K, Ho SY, Szentiks CA, Nikolin VM, Ma G, Damiani A, East ML, Lawrenz A, Hofer H, Osterrieder N. A potentially fatal mix of herpes in zoos.. Curr Biol 2012 Sep 25;22(18):1727-31.
              doi: 10.1016/j.cub.2012.07.035pubmed: 22902751google scholar: lookup
            9. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies.. Mol Biol Evol 2006 Feb;23(2):254-67.
              doi: 10.1093/molbev/msj030pubmed: 16221896google scholar: lookup
            10. Ibrahim ES, Kinoh M, Matsumura T, Kennedy M, Allen GP, Yamaguchi T, Fukushi H. Genetic relatedness and pathogenicity of equine herpesvirus 1 isolated from onager, zebra and gazelle.. Arch Virol 2007 Feb;152(2):245-55.
              doi: 10.1007/s00705-006-0855-3pubmed: 17051419google scholar: lookup
            11. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability.. Mol Biol Evol 2013 Apr;30(4):772-80.
              doi: 10.1093/molbev/mst010pmc: PMC3603318pubmed: 23329690google scholar: lookup
            12. Kennedy MA, Ramsay E, Diderrich V, Richman L, Allen GP, Potgieter LND. Encephalitis associated with a variant of equine herpesvirus 1 in a Thomson’s gazelle (Gazella thomsoni). J. Zoo Wildl. Med. 27: 533–538.
            13. Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2.. Nat Methods 2012 Mar 4;9(4):357-9.
              doi: 10.1038/nmeth.1923pmc: PMC3322381pubmed: 22388286google scholar: lookup
            14. Liu C, Guo W, Lu G, Xiang W, Wang X. Complete genomic sequence of an equine herpesvirus type 8 Wh strain isolated from China.. J Virol 2012 May;86(9):5407.
              doi: 10.1128/JVI.00445-12pmc: PMC3347380pubmed: 22492929google scholar: lookup
            15. Milne I, Wright F, Rowe G, Marshall DF, Husmeier D, McGuire G. TOPALi: software for automatic identification of recombinant sequences within DNA multiple alignments.. Bioinformatics 2004 Jul 22;20(11):1806-7.
              doi: 10.1093/bioinformatics/bth155pubmed: 14988107google scholar: lookup
            16. Montali RJ, Allen GP, Bryans JT, Phillips LG, Bush M. Equine herpesvirus type 1 abortion in an onager and suspected herpesvirus myelitis in a zebra.. J Am Vet Med Assoc 1985 Dec 1;187(11):1248-9.
              pubmed: 3001004
            17. Nugent J, Birch-Machin I, Smith KC, Mumford JA, Swann Z, Newton JR, Bowden RJ, Allen GP, Davis-Poynter N. Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks.. J Virol 2006 Apr;80(8):4047-60.
              doi: 10.1128/JVI.80.8.4047-4060.2006pmc: PMC1440451pubmed: 16571821google scholar: lookup
            18. Telford EA, Watson MS, McBride K, Davison AJ. The DNA sequence of equine herpesvirus-1.. Virology 1992 Jul;189(1):304-16.
              doi: 10.1016/0042-6822(92)90706-Upubmed: 1318606google scholar: lookup
            19. Telford EA, Watson MS, Perry J, Cullinane AA, Davison AJ. The DNA sequence of equine herpesvirus-4.. J Gen Virol 1998 May;79 ( Pt 5):1197-203.
              pubmed: 9603335doi: 10.1099/0022-1317-79-5-1197google scholar: lookup
            20. Volkening JD, Spatz SJ. Purification of DNA from the cell-associated herpesvirus Marek's disease virus for 454 pyrosequencing using micrococcal nuclease digestion and polyethylene glycol precipitation.. J Virol Methods 2009 Apr;157(1):55-61.
              doi: 10.1016/j.jviromet.2008.11.017pubmed: 19103224google scholar: lookup
            21. Wolff PL, Meehan TP, Basgall EJ, Allen GP, Sundberg JP. Abortion and perinatal foal mortality associated with equine herpesvirus type 1 in a herd of Grevy's zebra.. J Am Vet Med Assoc 1986 Nov 1;189(9):1185-6.
              pubmed: 2851577

            Citations

            This article has been cited 7 times.
            1. Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Roberts HC, Padalino B, Pasquali P, Spoolder H, Ståhl K, Calvo AV, Viltrop A, Winckler C, Carvelli A, Paillot R, Broglia A, Kohnle L, Baldinelli F, Van der Stede Y. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus-1.. EFSA J 2022 Jan;20(1):e07036.
              doi: 10.2903/j.efsa.2022.7036pubmed: 35035581google scholar: lookup
            2. Kang HW, Lee EY, Lee KK, Ko MK, Park JY, Kim YH, Lee K, Choi EJ, Kim J, So B, Park CK, Jeoung HY. Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea.. Pathogens 2021 Apr 2;10(4).
              doi: 10.3390/pathogens10040425pubmed: 33918404google scholar: lookup
            3. Preziuso S, Sgorbini M, Marmorini P, Cuteri V. Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes.. Viruses 2019 Sep 13;11(9).
              doi: 10.3390/v11090851pubmed: 31540321google scholar: lookup
            4. Moeller RB Jr, Crossley B, Pipkin A, Li Y, Balasuriya UBR. Systemic equid alphaherpesvirus 9 in a Grant's zebra.. J Vet Diagn Invest 2018 Jul;30(4):580-583.
              doi: 10.1177/1040638718767722pubmed: 29648506google scholar: lookup
            5. Garvey M, Suárez NM, Kerr K, Hector R, Moloney-Quinn L, Arkins S, Davison AJ, Cullinane A. Equid herpesvirus 8: Complete genome sequence and association with abortion in mares.. PLoS One 2018;13(2):e0192301.
              doi: 10.1371/journal.pone.0192301pubmed: 29414990google scholar: lookup
            6. Kolb AW, Lewin AC, Moeller Trane R, McLellan GJ, Brandt CR. Phylogenetic and recombination analysis of the herpesvirus genus varicellovirus.. BMC Genomics 2017 Nov 21;18(1):887.
              doi: 10.1186/s12864-017-4283-4pubmed: 29157201google scholar: lookup
            7. Abdelgawad A, Damiani A, Ho SY, Strauss G, Szentiks CA, East ML, Osterrieder N, Greenwood AD. Zebra Alphaherpesviruses (EHV-1 and EHV-9): Genetic Diversity, Latency and Co-Infections.. Viruses 2016 Sep 20;8(9).
              doi: 10.3390/v8090262pubmed: 27657113google scholar: lookup
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