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Toxins2016; 8(4); 89; doi: 10.3390/toxins8040089

Fumonisin B₁ (FB₁) Induces Lamellar Separation and Alters Sphingolipid Metabolism of In Vitro Cultured Hoof Explants.

Abstract: One of the most important hoof diseases is laminitis. Yet, the pathology of laminitis is not fully understood. Different bacterial toxins, e.g. endotoxins or exotoxins, seem to play an important role. Additionally, ingestion of mycotoxins, toxic secondary metabolites of fungi, might contribute to the onset of laminitis. In this respect, fumonsins are of special interest since horses are regarded as species most susceptible to this group of mycotoxins. The aim of our study was to investigate the influence of fumonisin B₁ (FB₁) on primary isolated epidermal and dermal hoof cells, as well as on the lamellar tissue integrity and sphingolipid metabolism of hoof explants in vitro. There was no effect of FB₁ at any concentration on dermal or epidermal cells. However, FB₁ significantly reduced the separation force of explants after 24 h of incubation. The Sa/So ratio was significantly increased in supernatants of explants incubated with FB₁ (2.5-10 µg/mL) after 24 h. Observed effects on Sa/So ratio were linked to significantly increased sphinganine concentrations. Our study showed that FB₁ impairs the sphingolipid metabolism of explants and reduces lamellar integrity at non-cytotoxic concentrations. FB₁ might, therefore, affect hoof health. Further in vitro and in vivo studies are necessary to elucidate the effects of FB₁ on the equine hoof in more detail.
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Publication Date: 2016-03-24 PubMed ID: 27023602PubMed Central: PMC4848616DOI: 10.3390/toxins8040089Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The abstract focuses on a study conducted to understand the effects of fumonisin B₁ (FB₁), a type of mycotoxin, on hoof health. This was particularly in relation to laminitis, a significant hoof disease. The study found that FB₁ affects sphingolipid metabolism and reduces lamellar integrity of hoof explants, implying that it could negatively impact hoof health.

Research Background

  • The study was concentrated around laminitis, a significant hoof disease, the pathology of which is not entirely understood.
  • It is suspected that bacterial toxins, such as endotoxins or exotoxins, play crucial roles in the onset of laminitis.
  • The research also considered the potential contribution of mycotoxins, toxic secondary metabolites of fungi, to laminitis.
  • Among mycotoxins, fumonisins were specifically examined because horses are generally more susceptible to this mycotoxin group.

Research Methods

  • The study investigated the effects of FB₁ on primary isolated epidermal and dermal hoof cells, as well as the integrity of the lamellar tissue and the metabolism of sphingolipids of hoof explants in vitro.
  • This allowed the researchers to consider FB₁’s potential impact on hoof health from multiple perspectives.

Research Findings

  • The study found no effect of FB₁ on dermal or epidermal cells in any concentration.
  • However, FB₁ significantly reduced the separation force of explants after 24 hours of incubation.
  • The Sa/So ratio in supernatants from explants incubated with FB₁ was significantly increased after 24 hours.
  • These effects were tied to significantly increased sphinganine concentrations, indicating altered sphingolipid metabolism.

Conclusions and Further Research

  • The study concluded that FB₁ impairs the sphingolipid metabolism of hoof explants and reduces lamellar integrity at non-cytotoxic concentrations, suggesting potential negative effects on hoof health.
  • The researchers recommend further in vitro and in vivo studies to understand the effects of FB₁ on the equine hoof more comprehensively.

Cite This Article

APA
Reisinger N, Dohnal I, Nagl V, Schaumberger S, Schatzmayr G, Mayer E. (2016). Fumonisin B₁ (FB₁) Induces Lamellar Separation and Alters Sphingolipid Metabolism of In Vitro Cultured Hoof Explants. Toxins (Basel), 8(4), 89. https://doi.org/10.3390/toxins8040089

Publication

Toxins
ISSN: 2072-6651
NlmUniqueID: 101530765
Country: Switzerland
Language: English
Volume: 8
Issue: 4
Pages: 89
PII: 89

Researcher Affiliations

Reisinger, Nicole
  • BIOMIN Research Center, Tulln 3430, Austria. nicole.reisinger@biomin.net.
Dohnal, Ilse
  • BIOMIN Research Center, Tulln 3430, Austria. ilse.dohnal@biomin.net.
Nagl, Veronika
  • BIOMIN Research Center, Tulln 3430, Austria. veronika.nagl@biomin.net.
Schaumberger, Simone
  • BIOMIN Research Center, Tulln 3430, Austria. simone.schaumberger@biomin.net.
Schatzmayr, Gerd
  • BIOMIN Research Center, Tulln 3430, Austria. gerd.schatzmayr@biomin.net.
Mayer, Elisabeth
  • BIOMIN Research Center, Tulln 3430, Austria. e.mayer@biomin.net.

MeSH Terms

  • Animals
  • Cell Survival / drug effects
  • Fumonisins / toxicity
  • Hoof and Claw / cytology
  • Hoof and Claw / drug effects
  • Hoof and Claw / metabolism
  • Hoof and Claw / pathology
  • Horses
  • Sphingolipids / metabolism

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Citations

This article has been cited 7 times.
  1. Reisinger N, Wendner D, Schauerhuber N, Mayer E. Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants. Animals (Basel) 2021 Jul 9;11(7).
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  2. Leung YH, Kenéz Á, Grob AJ, Feige K, Warnken T. Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses. J Vet Intern Med 2021 Jul;35(4):2009-2018.
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