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Veterinary sciences2023; 10(3); 218; doi: 10.3390/vetsci10030218

Galectins in Equine Placental Disease.

Abstract: Galectins are proteins that bind to glycans in targeted cells and function in cell-to-cell signaling throughout the body. Galectins have been found to be involved in various reproductive processes, including placental dysfunction, but this has not been investigated in the horse. Therefore, the objective of this study was to assess alterations in galectin expression of the abnormal placenta in pregnant mares. Next-generation RNA sequencing was performed on the postpartum chorioallantois of two placental pathologies following clinical cases of ascending placentitis (n = 7) and focal mucoid placentitis (n = 4), while chorioallantois from healthy postpartum pregnancies (n = 8; 4 control samples per disease group) served as the control. When evaluating ascending placentitis, both galectin-1 ( < 0.001) and galectin-3BP ( = 0.05) increased in the postpartum chorioallantois associated with disease, while galectin-8 ( < 0.0001) and galectin-12 ( < 0.01) decreased in the diseased chorioallantois in comparison with those in the control. In mares with focal mucoid placentitis, numerous galectins increased in the diseased chorioallantois, and this included galectin-1 ( < 0.01), galectin-3BP ( = 0.03), galectin-9 ( = 0.02), and galectin-12 ( = 0.04), in addition to a trend toward increases in galectin-3 ( = 0.08) and galectin-13 ( = 0.09). In contrast, galectin-8 expression decreased ( = 0.04) in the diseased chorioallantois in comparison with that of the controls. In conclusion, galectins alter in abnormal placentae with variations observed among two forms of placental pathologies. These cytokine-like proteins may further our understanding of placental pathophysiology and warrant attention as potential markers of placental inflammation and dysfunction in the horse.
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Publication Date: 2023-03-13 PubMed ID: 36977257PubMed Central: PMC10053007DOI: 10.3390/vetsci10030218Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how galectins, proteins involved in cell-to-cell signaling, change in abnormal horse placentas, especially ones associated with two forms of placental disease: ascending placentitis and focal mucoid placentitis.

Objective of the Study

  • The main goal of this research was to explore how galectin expression changes in the aberrant placentas of pregnant mares. This was achieved by examining the postpartum chorioallantois — a portion of the placenta — in cases of two specific placental conditions, contrasted with healthy pregnancies.

Methodology of the Study

  • The researchers leveraged next-generation RNA sequencing which is a powerful tool to detect RNA molecules and analyze gene expression.
  • The samples consisted of postpartum chorioallantois from clinical cases of ascending placentitis (7 instances) and focal mucoid placentitis (4 instances). The researchers also used chorioallantois from healthy postpartum pregnancies (8 instances) to serve as the control group.

Results of the Study

  • In instances of ascending placentitis, an increase in galectin-1 and galectin-3BP was observed in the chorioallantois associated with the disease, while decreased levels of galectin-8 and galectin-12 were recorded within the diseased samples in comparison to the controls.
  • Regarding the mares with focal mucoid placentitis, many galectins demonstrated increased levels in the chorioallantois including galectin-1, galectin-3BP, galectin-9, and galectin-12. They also reported a trend showing potential increases in galectin-3 and galectin-13. In contrast, the expression of galectin-8 decreased in the abnormal chorioallantois compared to that of the controls.

Conclusion of the Study

  • The results show that galectin expression alters in abnormal placentas among two types of placental pathologies. The research demonstrates that these changes could contribute further understanding to placental pathophysiology, and positions galectins as potential markers for placental inflammation and dysfunction in horses.

Cite This Article

APA
Fedorka CE, Ali HE, Troedsson MHT. (2023). Galectins in Equine Placental Disease. Vet Sci, 10(3), 218. https://doi.org/10.3390/vetsci10030218

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 10
Issue: 3
PII: 218

Researcher Affiliations

Fedorka, Carleigh E
  • Department of Veterinary Science, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY 40546, USA.
Ali, Hossam El-Sheikh
  • Department of Veterinary Science, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY 40546, USA.
  • Department of Theriogenology, College of Veterinary Medicine, Mansoura University, Dakahlia 35516, Egypt.
Troedsson, Mats H T
  • Department of Veterinary Science, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY 40546, USA.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 40 references
  1. Rabinovich GA, Toscano MA, Jackson SS, Vasta GR. Functions of cell surface galectin-glycoprotein lattices.. Curr Opin Struct Biol 2007 Oct;17(5):513-20.
    doi: 10.1016/j.sbi.2007.09.002pmc: PMC2100406pubmed: 17950594google scholar: lookup
  2. Hernandez JD, Baum LG. Ah, sweet mystery of death! Galectins and control of cell fate.. Glycobiology 2002 Oct;12(10):127R-36R.
    doi: 10.1093/glycob/cwf081pubmed: 12244068google scholar: lookup
  3. Blois SM, Ilarregui JM, Tometten M, Garcia M, Orsal AS, Cordo-Russo R, Toscano MA, Bianco GA, Kobelt P, Handjiski B, Tirado I, Markert UR, Klapp BF, Poirier F, Szekeres-Bartho J, Rabinovich GA, Arck PC. A pivotal role for galectin-1 in fetomaternal tolerance.. Nat Med 2007 Dec;13(12):1450-7.
    doi: 10.1038/nm1680pubmed: 18026113google scholar: lookup
  4. Kolundžić N, Bojić-Trbojević Ž, Kovačević T, Stefanoska I, Kadoya T, Vićovac L. Galectin-1 is part of human trophoblast invasion machinery--a functional study in vitro.. PLoS One 2011;6(12):e28514.
    doi: 10.1371/journal.pone.0028514pmc: PMC3234277pubmed: 22174828google scholar: lookup
  5. Kolundžić N, Bojić-Trbojević Z, Radojčić Lj, Petronijević M, Vićovac Lj. Galectin-8 is expressed by villous and extravillous trophoblast of the human placenta.. Placenta 2011 Nov;32(11):909-11.
    doi: 10.1016/j.placenta.2011.07.087pubmed: 21862124google scholar: lookup
  6. Hirota Y, Burnum KE, Acar N, Rabinovich GA, Daikoku T, Dey SK. Galectin-1 markedly reduces the incidence of resorptions in mice missing immunophilin FKBP52.. Endocrinology 2012 May;153(5):2486-93.
    doi: 10.1210/en.2012-1035pmc: PMC3339653pubmed: 22416080google scholar: lookup
  7. Alese OM, Moodley J, Naicker T. The role of Galectin-1 in HIV associated preeclampsia.. Eur J Obstet Gynecol Reprod Biol 2020 Mar;246:138-144.
    doi: 10.1016/j.ejogrb.2020.01.024pubmed: 32018196google scholar: lookup
  8. Charkiewicz K, Goscik J, Raba G, Laudanski P. Syndecan 4, galectin 2, and death receptor 3 (DR3) as novel proteins in pathophysiology of preeclampsia.. J Matern Fetal Neonatal Med 2021 Sep;34(18):2965-2970.
    doi: 10.1080/14767058.2019.1676410pubmed: 31608721google scholar: lookup
  9. Li ZH, Wang LL, Liu H, Muyayalo KP, Huang XB, Mor G, Liao AH. Galectin-9 Alleviates LPS-Induced Preeclampsia-Like Impairment in Rats via Switching Decidual Macrophage Polarization to M2 Subtype.. Front Immunol 2018;9:3142.
    doi: 10.3389/fimmu.2018.03142pmc: PMC6335255pubmed: 30687334google scholar: lookup
  10. Than NG, Kim SS, Abbas A, Han YM, Hotra J, Tarca AL, Erez O, Wildman DE, Kusanovic JP, Pineles B, Montenegro D, Edwin SS, Mazaki-Tovi S, Gotsch F, Espinoza J, Hassan SS, Papp Z, Romero R. Chorioamnionitis and increased galectin-1 expression in PPROM --an anti-inflammatory response in the fetal membranes?. Am J Reprod Immunol 2008 Oct;60(4):298-311.
    doi: 10.1111/j.1600-0897.2008.00624.xpmc: PMC2784815pubmed: 18691335google scholar: lookup
  11. Stefanoska I, Tadić J, Vilotić A, Jovanović Krivokuća M, Abu Rabi T, Vićovac L. Histological chorioamnionitis in preterm prelabor rupture of the membranes is associated with increased expression of galectin-3 by amniotic epithelium.. J Matern Fetal Neonatal Med 2017 Sep;30(18):2232-2236.
    doi: 10.1080/14767058.2016.1243100pubmed: 27690725google scholar: lookup
  12. Kaya B, Turhan U, Sezer S, Kaya S, Dağ İ, Tayyar A. Maternal serum galectin-1 and galectin-3 levels in pregnancies complicated with preterm prelabor rupture of membranes.. J Matern Fetal Neonatal Med 2020 Mar;33(5):861-868.
    doi: 10.1080/14767058.2019.1637409pubmed: 31242786google scholar: lookup
  13. He M, Jiang M, Zhou Y, Li F, Yang M, Fan Y, Xie Y, Beejadhursing R, Feng L, Deng D. Impaired Gal-9 Dysregulates the PBMC-Induced Th1/Th2 Imbalance in Abortion-Prone Matings.. J Immunol Res 2018;2018:9517842.
    doi: 10.1155/2018/9517842pmc: PMC5831994pubmed: 29651447google scholar: lookup
  14. Wu M, Liu P, Cheng L. Galectin-1 reduction and changes in T regulatory cells may play crucial roles in patients with unexplained recurrent spontaneous abortion.. Int J Clin Exp Pathol 2015;8(2):1973-8.
    pmc: PMC4396310pubmed: 25973091
  15. Tirado-González I, Freitag N, Barrientos G, Shaikly V, Nagaeva O, Strand M, Kjellberg L, Klapp BF, Mincheva-Nilsson L, Cohen M, Blois SM. Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy.. Mol Hum Reprod 2013 Jan;19(1):43-53.
    doi: 10.1093/molehr/gas043pubmed: 23002109google scholar: lookup
  16. Yalçin I, Taşkin S, Pabuçcu EG, Söylemez F. The value of placental protein 13, β-human chorionic gonadotropin and progesterone in the prediction of miscarriages in threatened miscarriage patients.. J Obstet Gynaecol 2015 Apr;35(3):283-6.
    doi: 10.3109/01443615.2014.948822pubmed: 25153203google scholar: lookup
  17. Fedorka CE, Ali HE, Troedsson MHT. Galectinology of Equine Pregnancy.. Animals (Basel) 2022 Dec 29;13(1).
    doi: 10.3390/ani13010129pmc: PMC9817698pubmed: 36611738google scholar: lookup
  18. Giles RC, Donahue JM, Hong CB, Tuttle PA, Petrites-Murphy MB, Poonacha KB, Roberts AW, Tramontin RR, Smith B, Swerczek TW. Causes of abortion, stillbirth, and perinatal death in horses: 3,527 cases (1986-1991).. J Am Vet Med Assoc 1993 Oct 15;203(8):1170-5.
    pubmed: 8244867
  19. Hong CB, Donahue JM, Giles RC Jr, Petrites-Murphy MB, Poonacha KB, Roberts AW, Smith BJ, Tramontin RR, Tuttle PA, Swerczek TW. Equine abortion and stillbirth in central Kentucky during 1988 and 1989 foaling seasons.. J Vet Diagn Invest 1993 Oct;5(4):560-6.
    doi: 10.1177/104063879300500410pubmed: 8286455google scholar: lookup
  20. Ali HE, Ball BA, Fedorka CE, Scoggin KE, Schnobrich MR, Erol E, Ruby RE, Loynachan AT, Smith JL, Carter CN. Nocardioform Placentitis: A Continuing Question. Proceedings of the American Association of Equine Practitioners Nashville, TN, USA. 4–8 December 2021.
  21. El-Sheikh Ali H, Loux SC, Kennedy L, Scoggin KE, Dini P, Fedorka CE, Kalbfleisch TS, Esteller-Vico A, Horohov DW, Erol E, Carter CN, Smith JL, Ball BA. Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis.. Vet Res 2021 Jul 8;52(1):103.
    doi: 10.1186/s13567-021-00972-4pmc: PMC8268225pubmed: 34238364google scholar: lookup
  22. Canisso IF, Ball BA, Erol E, Claes A, Scoggin KE, McDowell KJ, Williams NM, Dorton AR, Wolfsdorf KE, Squires EL, Troedsson MH. Attempts to induce nocardioform placentitis (Crossiela equi) experimentally in mares.. Equine Vet J 2015 Jan;47(1):91-5.
    doi: 10.1111/evj.12249pubmed: 24612109google scholar: lookup
  23. Renaudin CD, Troedsson MH, Gillis CL, King VL, Bodena A. Ultrasonographic evaluation of the equine placenta by transrectal and transabdominal approach in the normal pregnant mare.. Theriogenology 1997 Jan 15;47(2):559-73.
    doi: 10.1016/S0093-691X(97)00014-9pubmed: 16728008google scholar: lookup
  24. LeBlanc MM. Ascending placentitis in the mare: an update.. Reprod Domest Anim 2010 Jun;45 Suppl 2:28-34.
    doi: 10.1111/j.1439-0531.2010.01633.xpubmed: 20591062google scholar: lookup
  25. El-Sheikh Ali H, Dini P, Scoggin K, Loux S, Fedorka C, Boakari Y, Norris J, Esteller-Vico A, Kalbfleisch T, Ball B. Transcriptomic analysis of equine placenta reveals key regulators and pathways involved in ascending placentitis†.. Biol Reprod 2021 Mar 11;104(3):638-656.
    doi: 10.1093/biolre/ioaa209pubmed: 33345276google scholar: lookup
  26. Dobin A, Gingeras TR. Mapping RNA-seq Reads with STAR.. Curr Protoc Bioinformatics 2015 Sep 3;51:11.14.1-11.14.19.
    doi: 10.1002/0471250953.bi1114s51pmc: PMC4631051pubmed: 26334920google scholar: lookup
  27. Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL, Pachter L. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks.. Nat Protoc 2012 Mar 1;7(3):562-78.
    doi: 10.1038/nprot.2012.016pmc: PMC3334321pubmed: 22383036google scholar: lookup
  28. Murase H, El-Sheikh Ali H, Ruby RE, Scoggin KE, Ball BA. Transcriptomic analysis of the chorioallantois in equine premature placental separation.. Equine Vet J 2023 May;55(3):405-418.
    doi: 10.1111/evj.13602pubmed: 35622344google scholar: lookup
  29. Hong CB, Donahue JM, Giles RC Jr, Petrites-Murphy MB, Poonacha KB, Roberts AW, Smith BJ, Tramontin RR, Tuttle PA, Swerczek TW. Etiology and pathology of equine placentitis.. J Vet Diagn Invest 1993 Jan;5(1):56-63.
    doi: 10.1177/104063879300500113pubmed: 8466982google scholar: lookup
  30. Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D. Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.. Am J Pathol 2011 Mar;178(3):1201-9.
    doi: 10.1016/j.ajpath.2010.11.073pmc: PMC3069826pubmed: 21356371google scholar: lookup
  31. DeRoo EP, Wrobleski SK, Shea EM, Al-Khalil RK, Hawley AE, Henke PK, Myers DD Jr, Wakefield TW, Diaz JA. The role of galectin-3 and galectin-3-binding protein in venous thrombosis.. Blood 2015 Mar 12;125(11):1813-21.
    doi: 10.1182/blood-2014-04-569939pmc: PMC4357586pubmed: 25428218google scholar: lookup
  32. El-Sheikh Ali H, Boakari YL, Loux SC, Dini P, Scoggin KE, Esteller-Vico A, Kalbfleisch T, Ball BA. Transcriptomic analysis reveals the key regulators and molecular mechanisms underlying myometrial activation during equine placentitis†.. Biol Reprod 2020 May 26;102(6):1306-1325.
    doi: 10.1093/biolre/ioaa020pubmed: 32065222google scholar: lookup
  33. Kavanaugh D, Kane M, Joshi L, Hickey RM. Detection of galectin-3 interaction with commensal bacteria.. Appl Environ Microbiol 2013 Jun;79(11):3507-10.
    doi: 10.1128/AEM.03694-12pmc: PMC3648028pubmed: 23524672google scholar: lookup
  34. Hao H, He M, Li J, Zhou Y, Dang J, Li F, Yang M, Deng D. Upregulation of the Tim-3/Gal-9 pathway and correlation with the development of preeclampsia.. Eur J Obstet Gynecol Reprod Biol 2015 Nov;194:85-91.
    doi: 10.1016/j.ejogrb.2015.08.022pubmed: 26342682google scholar: lookup
  35. Li J, Li FF, Zuo W, Zhou Y, Hao HY, Dang J, Jiang M, He MZ, Deng DR. Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion.. J Huazhong Univ Sci Technolog Med Sci 2014 Aug;34(4):586-590.
    doi: 10.1007/s11596-014-1320-2pubmed: 25135732google scholar: lookup
  36. Li Y, Zhang J, Zhang D, Hong X, Tao Y, Wang S, Xu Y, Piao H, Yin W, Yu M, Zhang Y, Fu Q, Li D, Chang X, Du M. Tim-3 signaling in peripheral NK cells promotes maternal-fetal immune tolerance and alleviates pregnancy loss.. Sci Signal 2017 Sep 26;10(498).
    doi: 10.1126/scisignal.aah4323pubmed: 28951537google scholar: lookup
  37. Burger O, Pick E, Zwickel J, Klayman M, Meiri H, Slotky R, Mandel S, Rabinovitch L, Paltieli Y, Admon A, Gonen R. Placental protein 13 (PP-13): effects on cultured trophoblasts, and its detection in human body fluids in normal and pathological pregnancies.. Placenta 2004 Aug;25(7):608-22.
    doi: 10.1016/j.placenta.2003.12.009pubmed: 15193867google scholar: lookup
  38. Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernández MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT. Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.. FASEB J 2011 Jan;25(1):242-54.
    doi: 10.1096/fj.09-144907pubmed: 20876211google scholar: lookup
  39. Cheng YL, Wu YW, Kuo CF, Lu SL, Liu FT, Anderson R, Lin CF, Liu YL, Wang WY, Chen YD, Zheng PX, Wu JJ, Lin YS. Galectin-3 Inhibits Galectin-8/Parkin-Mediated Ubiquitination of Group A Streptococcus.. mBio 2017 Jul 25;8(4).
    doi: 10.1128/mBio.00899-17pmc: PMC5527311pubmed: 28743815google scholar: lookup
  40. Wan L, Lin HJ, Huang CC, Chen YC, Hsu YA, Lin CH, Lin HC, Chang CY, Huang SH, Lin JM, Liu FT. Galectin-12 enhances inflammation by promoting M1 polarization of macrophages and reduces insulin sensitivity in adipocytes.. Glycobiology 2016 Jul;26(7):732-744.
    doi: 10.1093/glycob/cww013pubmed: 26873172google scholar: lookup

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