[Ganglioside GM3 from horse erythrocytes: structure and effect on cell proliferation].
Abstract: An increase of the mouse fibroblast proliferation by ganglioside GM3 from equine erythrocytes is described. The structure of GM3 has been established on the basis of chemical methods, enzymatic degradation, GC-MS, as well as plasma desorption mass spectrometry and HPLC of 9-anthrylmethyl esters of gangliosides to characterize the long-chain base composition. The oligosaccharide moiety includes an N-glycolylneuraminic acid residue, whereas the main components of the lipid moiety are 20:1 sphingosine and 24:0 fatty acids.
Publication Date: 1993-08-01 PubMed ID: 8379955
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- English Abstract
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research examines the influence of GM3 ganglioside derived from horse red blood cells on the proliferation of mouse fibroblasts, identifying the structure of GM3 through various analysis methods and detailing its components.
Objective of the Research
- The study aimed to understand the structure and effects of GM3 ganglioside from equine erythrocytes (horse red blood cells) on the proliferation (growth and multiplication) of mouse fibroblasts (specific collagen-producing cells).
Methods Used for Analysis
- The structure of GM3 was established using multiple methods. This included chemical methods, enzymatic degradation (breaking down of the substance using enzymes), GC-MS (Gas Chromatography-Mass Spectrometry, a method for analyzing and separating chemicals), plasma desorption mass spectrometry (a type of mass spectrometry that uses plasma to ionize the sample), and HPLC (High Performance Liquid Chromatography, a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture).
- 9-anthrylmethyl esters of gangliosides were used to identify the long-chain base composition of the substance.
Findings Regarding the Structure of GM3
- The oligosaccharide part of the GM3 includes an N-glycolylneuraminic acid residue. Oligosaccharides are carbohydrates that consist of a small number of simple sugars (monosaccharides).
- The primary components of the lipid portion of GM3 are 20:1 sphingosine and 24:0 fatty acids. Lipids are organic compounds that are fatty acids or their derivatives and are insoluble in water but soluble in organic solvents. They include many natural oils, waxes, and steroids. Sphingosine is a type of lipid and found in cell membranes.
Effects of GM3 on Cell Proliferation
- The researchers found that the GM3 ganglioside from equine erythrocytes caused an increase in the proliferation of mouse fibroblasts. This suggests that GM3 plays a significant role in cellular growth and replication, at least in the context of these specific cells.
Cite This Article
APA
Menzeleev RF, Smirnova GP, Chekareva NV, Zvonkova EN, Krasnopol'skiĭ IuM, Shvets VI.
(1993).
[Ganglioside GM3 from horse erythrocytes: structure and effect on cell proliferation].
Bioorg Khim, 19(8), 817-824.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Carbohydrate Conformation
- Carbohydrate Sequence
- Cell Division / drug effects
- Cells, Cultured
- Chromatography, High Pressure Liquid
- Erythrocytes / drug effects
- G(M3) Ganglioside / chemistry
- G(M3) Ganglioside / pharmacology
- Gas Chromatography-Mass Spectrometry
- Horses
- Molecular Sequence Data
Citations
This article has been cited 2 times.- Davies LR, Varki A. Why Is N-Glycolylneuraminic Acid Rare in the Vertebrate Brain?. Top Curr Chem 2015;366:31-54.
- Davies LR, Pearce OM, Tessier MB, Assar S, Smutova V, Pajunen M, Sumida M, Sato C, Kitajima K, Finne J, Gagneux P, Pshezhetsky A, Woods R, Varki A. Metabolism of vertebrate amino sugars with N-glycolyl groups: resistance of α2-8-linked N-glycolylneuraminic acid to enzymatic cleavage. J Biol Chem 2012 Aug 17;287(34):28917-31.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
