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Home / Equine Research Bank / J Vet Intern Med / Gastric microbiome in horses with and without equine glandul...
Journal of veterinary internal medicine2021; 35(5); 2458-2464; doi: 10.1111/jvim.16241

Gastric microbiome in horses with and without equine glandular gastric disease.

Abstract: The role of the gastric microbiome in development or persistence of equine glandular gastric disease (EGGD) remains to be investigated. Objective: The objective was to characterize the glandular mucosal and gastric fluid microbiomes of horses with and without EGGD. It was hypothesized that differences in the mucosal microbiome are associated with EGGD. Methods: Twenty-four horses were enrolled. Methods: Gastroscopy was performed and EGGD scores recorded (score 0, n = 6; score 1, n = 8; score ≥2, n = 10). Gastric fluid and pinch biopsies of healthy glandular mucosa and EGGD lesions were collected via gastroscope. 16S rRNA amplicon sequencing of the gastric fluid and glandular mucosal biopsies was performed. Relationships between gastric fluid and mucosal microbial community composition were evaluated among EGGD score groups (EGGD 0-BX, EGGD 1-BX, EGGD ≥2-BX) and among endoscopic appearances: controls from horses without EGGD and normal areas, hyperemic areas, and lesions from horses with EGGD. Results: Microbial community structure of mucosal biopsies differed among EGGD score groups (Jaccard similarity index; P = .009). Principal coordinate analysis showed separate clusters for EGGD 0-BX and EGGD ≥2-BX. Conclusions: A modest difference was detected in the community structure of the gastric glandular mucosal microbiome in association with EGGD score.
© 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2021-08-05 PubMed ID: 34351018PubMed Central: PMC8478018DOI: 10.1111/jvim.16241Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper aims to investigate the differences in the gastric microbiome of horses having equine glandular gastric disease (EGGD) and those not having the disease. The study revealed slight variations in the structure of the gastric glandular mucosal microbiome in horses with varying severity of EGGD.

Objective of the Study

  • The primary goal of the study was to determine and analyze the charactersitics of the glandular mucosal and gastric fluid microbiomes in horses suffering from EGGD and those not suffering from it.
  • The researchers hypothesized that EGGD is likely to be associated with differences in the mucosal microbiome.

Methodology

  • The researchers enrolled twenty-four horses in the study and performed gastroscopy to record the EGGD scores.
  • They divided the horses into three categories based on their EGGD score: score 0, score 1, and score ≥2.
  • Gastric fluid and pinch biopsies of healthy glandular mucosa and EGGD lesions were collected using a gastroscope.
  • The team performed 16S rRNA amplicon sequencing on the collected gastric fluid and glandular mucosal biopsies.
  • They evaluated the relationships between gastric fluid and mucosal microbial community composition among the EGGD score groups and among different endoscopic appearances: controls from horses without EGGD and normal areas, hyperemic areas, and lesions from horses suffering with EGGD.

Results

  • The findings from the research suggested a difference in the microbial community structure of mucosal biopsies among different EGGD score groups. This was established through Jaccard similarity index.
  • Using principal coordinate analysis, separate clusters for EGGD 0-BX and EGGD ≥2-BX were discovered.

Conclusion

  • The research concluded that a modest difference could be spotted in the community structure of the gastric glandular mucosal microbiome in association with EGGD score.
  • While the finding does support the initial hypothesis of the study, the differences uncovered were modest and suggest that further research may be needed to fully understand the role of the gastric microbiome in the development and persistence of EGGD in horses.

Cite This Article

APA
Paul LJ, Ericsson AC, Andrews FM, Keowen ML, Morales Yniguez F, Garza F, Banse HE. (2021). Gastric microbiome in horses with and without equine glandular gastric disease. J Vet Intern Med, 35(5), 2458-2464. https://doi.org/10.1111/jvim.16241

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 35
Issue: 5
Pages: 2458-2464

Researcher Affiliations

Paul, Linda J
  • Louisiana State University, Veterinary Clinical Sciences, Equine Health Studies Program, Baton Rouge, Louisiana, USA.
Ericsson, Aaron C
  • Metagenomics Center, Equine Gut Group, University of Missouri, Columbia, Missouri, USA.
Andrews, Frank M
  • Louisiana State University, Veterinary Clinical Sciences, Equine Health Studies Program, Baton Rouge, Louisiana, USA.
Keowen, Michael L
  • Louisiana State University, Veterinary Clinical Sciences, Equine Health Studies Program, Baton Rouge, Louisiana, USA.
Morales Yniguez, Francisco
  • Louisiana State University, Veterinary Clinical Sciences, Equine Health Studies Program, Baton Rouge, Louisiana, USA.
Garza, Frank
  • Louisiana State University, Veterinary Clinical Sciences, Equine Health Studies Program, Baton Rouge, Louisiana, USA.
Banse, Heidi E
  • Louisiana State University, Veterinary Clinical Sciences, Equine Health Studies Program, Baton Rouge, Louisiana, USA.

MeSH Terms

  • Animals
  • Gastric Mucosa
  • Gastrointestinal Microbiome
  • Horse Diseases
  • Horses
  • RNA, Ribosomal, 16S / genetics
  • Stomach Diseases / veterinary
  • Stomach Ulcer / veterinary

Grant Funding

  • LSU Equine Health Studies Program Charles V. Cusimano Grant

Conflict of Interest Statement

Authors declare no conflict of interest.

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Citations

This article has been cited 5 times.
  1. Zhao Y, Ren X, Wu H, Hu H, Cheng C, Du M, Huang Y, Zhao X, Wang L, Yi L, Tao J, Li Y, Lin Y, Su S, Dugarjaviin M. Diversity and functional prediction of fungal communities in different segments of mongolian horse gastrointestinal tracts.. BMC Microbiol 2023 Sep 9;23(1):253.
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  2. Julliand S, Buttet M, Hermange T, Hillon P, Julliand V. Effect of diet composition on glandular gastric disease in horses.. J Vet Intern Med 2023 Jul-Aug;37(4):1528-1536.
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  4. Chaucheyras-Durand F, Sacy A, Karges K, Apper E. Gastro-Intestinal Microbiota in Equines and Its Role in Health and Disease: The Black Box Opens.. Microorganisms 2022 Dec 19;10(12).
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