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Home / Equine Research Bank / Acta Vet Scand / Gelatinolytic activity in tracheal aspirates of horses with ...
Acta veterinaria Scandinavica1997; 38(1); 17-27; doi: 10.1186/BF03548504

Gelatinolytic activity in tracheal aspirates of horses with chronic obstructive pulmonary disease.

Abstract: The gelatinolytic activity in tracheal aspirates (TA) of horses with chronic obstructive pulmonary disease (COPD) was analyzed using SDS-PAGE-gelatin-gel electrophoresis (zymography) and compared to TAs from healthy controls. The 110-90 kD MMP-9 type gelatinase was high in symptomatic disease phases (permanent disease 0.46 +/- 0.15, p < 0.001; or intermittent disease 0.47 +/- 0.12, p < 0.001) compared to healthy controls (0.10 +/- 0.07). Similarly, the overall gelatinolytic activity, the activity in high-mw gelatinolytic bands (210-190 and 150 kD) and in proteolytically processed fragments in the 50-40 kD range were high, whereas the 75-65 kD MMP-2 was not altered. These findings suggest that MMP-9 type gelatinases, originating possibly from neutrophils or macrophages, and products thereof have a role in the pathogenesis of equine respiratory diseases, whereas MMP-2 type gelatinases represent house-keeping proteinases involved with normal tissue remodelling. The gelatinolytic activity in TAs correlated with the beta-glucuronidase activity, which indicates that they are simultaneously elevated in the respiratory secretions of horses suffering from COPD and might both be of same origin, or have a causal relationship.
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Publication Date: 1997-01-01 PubMed ID: 9129343PubMed Central: PMC8057023DOI: 10.1186/BF03548504Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on the study of gelatinolytic activity in the tracheal aspirates (TA) of horses affected with chronic obstructive pulmonary disease (COPD) and comparing it with the TA from healthy horses. The results indicate higher activity of MMP-9 type gelatinases and overall gelatinolytic activity in horses with COPD than in healthy ones.

About the Study

  • This research utilized an analytical procedure called SDS-PAGE-gelatin-gel electrophoresis (zymography) to analyze the gelatinolytic activity in TAs of horses with COPD. Gelatinolytic enzymes are enzymes that break down gelatin, a derivative of collagen, which is a major component of the extracellular matrix in animal tissues.
  • The researchers used horses suffering from two different types of COPD – permanent and intermittent – and compared the results with healthy controls. Gelatinolytic activity was found to be higher in COPD-affected horses, specifically MMP-9 type gelatinase was presented in high amounts.

Main Findings

  • The researchers discovered that the gelatinases, particularly the 110-90 kD MMP-9 type, had high activity in symptomatic disease phases of COPD. The MMP-9 type gelatinase levels were significantly higher in COPD-affected horses compared with healthy controls.
  • The overall gelatinolytic activity was also higher in COPD-affected horses, as well as the activity in high-molecular-weight gelatinolytic bands and proteolytically processed fragments in the 50-40 kD range. However, the 75-65 kD MMP-2 gelatinase was not altered, indicating that this type is associated mainly with normal tissue remodelling.
  • These findings suggest that MMP-9 type gelatinases, possibly originating from neutrophils or macrophages, and their products, play a crucial role in the pathogenesis of equine respiratory diseases like COPD.
  • In addition, the researchers found a correlation between the gelatinolytic activity in TAs and the beta-glucuronidase activity, suggesting that the two are simultaneously increased in the respiratory secretions of horses with COPD. This could indicate a common origin or a causal relationship between the two activities.

Implications

  • This study provides crucial insights into the role of gelatinolytic activity in equine COPD. Enhanced understanding of the role of different gelatinases, especially MMP-9 type, in COPD may pave the way for new therapeutic strategies to treat the disease.
  • The correlation between gelatinolytic activity and beta-glucuronidase activity also sheds light on the possible mechanisms involved in the pathogenesis of COPD. However, more detailed studies will be needed to fully understand how these two activities are related and their exact roles in disease development.

Cite This Article

APA
Koivunen AL, Maisi P, Konttinen YT, Sandholm M. (1997). Gelatinolytic activity in tracheal aspirates of horses with chronic obstructive pulmonary disease. Acta Vet Scand, 38(1), 17-27. https://doi.org/10.1186/BF03548504

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 38
Issue: 1
Pages: 17-27

Researcher Affiliations

Koivunen, A L
  • Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Maisi, P
    Konttinen, Y T
      Sandholm, M

        MeSH Terms

        • Animals
        • Collagenases / metabolism
        • Gelatin / metabolism
        • Gelatinases / isolation & purification
        • Gelatinases / metabolism
        • Glucuronidase / metabolism
        • Horse Diseases
        • Horses
        • Lung Diseases, Obstructive / enzymology
        • Lung Diseases, Obstructive / veterinary
        • Matrix Metalloproteinase 2
        • Matrix Metalloproteinase 9
        • Metalloendopeptidases / metabolism
        • Mucous Membrane / enzymology
        • Suction
        • Trachea

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        Citations

        This article has been cited 8 times.
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        2. Barton AK, Shety T, Klier J, Geis S, Einspanier R, Gehlen H. Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma. Mediators Inflamm 2019;2019:7845623.
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        3. Barton AK, Gehlen H. Pulmonary Remodeling in Equine Asthma: What Do We Know about Mediators of Inflammation in the Horse?. Mediators Inflamm 2016;2016:5693205.
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        4. Barton AK, Shety T, Bondzio A, Einspanier R, Gehlen H. Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy in combination with environmental dust reduction in equine recurrent airway obstruction. BMC Vet Res 2016 Dec 9;12(1):282.
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        5. Barton AK, Shety T, Bondzio A, Einspanier R, Gehlen H. Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse. Mediators Inflamm 2015;2015:569512.
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        8. Koivunen AL, Maisi P, Konttinen YT, Prikk K, Sandholm M. Collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates of horses with chronic obstructive pulmonary disease. Acta Vet Scand 1997;38(1):9-16.
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