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Home / Equine Research Bank / J Vet Med Sci / Genetic analysis of equine methicillin-resistant Staphylococ...
The Journal of veterinary medical science1997; 59(10); 935-937; doi: 10.1292/jvms.59.935

Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis.

Abstract: Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.
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Publication Date: 1997-11-15 PubMed ID: 9362045DOI: 10.1292/jvms.59.935Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the genetic relationships of 15 methicillin-resistant Staphylococcus aureus samples from horses using a technique called pulsed-field gel electrophoresis.

Methodology – Pulsed-Field Gel Electrophoresis (PFGE)

  • The researchers used PFGE, a laboratory technique used for the separation of large DNA molecules by applying to a gel matrix an electric field that periodically changes direction.
  • It allows the differentiation of bacterial strains at a molecular level and is often used in investigations of disease outbreaks to determine the source and spread of the specific bacteria.

Sample Origin and Characteristics

  • The studied samples originated from mares suffering from metritis (an inflammation of the uterus) and a stallion diagnosed with dermatitis, in a region in Japan called Hokkaido.
  • All samples showed a phage pattern of 6/47/54/75, which refers to bacteriophage types/isolates, an important factor in the ecology and pathogenicity of S. aureus.
  • All samples were categorized as coagulase type IV and enterotoxin type A, both key virulence factors determining the disease-causing ability of the S. aureus bacteria.

Genetic Analysis

  • The researchers used restriction endonuclease SmaI, an enzyme that cuts DNA into smaller fragments, to divide the genomic DNA of each sample into 15 or 16 fragments.
  • The resulting DNA sizes ranged from 8 to 630 kilobase pairs (kb). The size range suggests high genomic diversity among the samples, an important factor in understanding the evolution and adaptation ability of the bacteria.

Comparison of Isolates and Results

  • Out of the 15 isolates, 14 shared the same PFGE pattern, indicating strong genetic similarity and possibly a shared origin.
  • The remaining isolate had a closely related but different PFGE pattern, suggesting a certain level of genetic variation or potentially a different development path.
  • When compared to 9 human MRSA isolates that exhibited the same phenotypic characteristics, the horse isolates showed different PFGE patterns, underscoring potential species specificity of genomic variations.

Cite This Article

APA
Shimizu A, Kawano J, Yamamoto C, Kakutani O, Anzai T, Kamada M. (1997). Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis. J Vet Med Sci, 59(10), 935-937. https://doi.org/10.1292/jvms.59.935

Publication

The Journal of veterinary medical science
ISSN: 0916-7250
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 59
Issue: 10
Pages: 935-937

Researcher Affiliations

Shimizu, A
  • Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Hyogo, Japan.
Kawano, J
    Yamamoto, C
      Kakutani, O
        Anzai, T
          Kamada, M

            MeSH Terms

            • Animals
            • DNA, Bacterial / analysis
            • DNA, Bacterial / chemistry
            • DNA, Bacterial / genetics
            • Dermatitis / microbiology
            • Dermatitis / pathology
            • Dermatitis / veterinary
            • Electrophoresis, Gel, Pulsed-Field / methods
            • Electrophoresis, Gel, Pulsed-Field / veterinary
            • Endometritis / microbiology
            • Endometritis / pathology
            • Endometritis / veterinary
            • Female
            • Horse Diseases / microbiology
            • Horse Diseases / pathology
            • Horses
            • Humans
            • Male
            • Methicillin Resistance / genetics
            • Phenotype
            • Skin / microbiology
            • Skin / pathology
            • Staphylococcal Infections / microbiology
            • Staphylococcal Infections / pathology
            • Staphylococcal Infections / veterinary
            • Staphylococcus aureus / drug effects
            • Staphylococcus aureus / genetics
            • Staphylococcus aureus / isolation & purification
            • Uterus / microbiology
            • Uterus / pathology

            Citations

            This article has been cited 16 times.
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