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Archives of virology2017; 162(8); 2329-2335; doi: 10.1007/s00705-017-3376-3

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland.

Abstract: Equid herpesvirus type 1 (EHV-1) is a common viral infection associated with varied clinical outcomes including respiratory disease, abortion and neurological disease. We have characterized EHV-1 sequences (n = 38) obtained from cases of equine abortion in Poland between 1999 and 2016, based on sequencing of PCR products from open reading frames (ORF) 30 and 68 of the EHV-1 genome. The majority (81.6%) of sequences were not classified into any of the previously described groups based on the ORF68 sequence. The remaining sequences belonged to ORF68 group III (7.9%) or IV (10.5%). A haplotype network analysis did not show any obvious structure within networks of local Polish sequences, nor within a global network of 215 EHV-1 sequences when these networks were coloured based on the geographical origin of viruses or date of detection. Our data suggest that ORF68 does not provide a reliable molecular marker for epidemiological studies of EHV-1, at least in a global sense. Its usefulness to aid local investigations of individual outbreaks remains to be established. All but two Polish EHV-1 sequences belonged to the ORF30 N genotype. The two ORF30 D viruses were obtained from abortion cases in 2009 and 2010. Hence, abortion cases that occurred in Poland between 1999 and 2016 were caused predominantly by EHV-1 with the ORF30 N genotype, with no indication of an increase in the prevalence of the ORF30 D variant.
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Publication Date: 2017-04-27 PubMed ID: 28451902PubMed Central: PMC5506511DOI: 10.1007/s00705-017-3376-3Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the genetic characterization of Equid herpesvirus type 1 (EHV-1), a common virus causing various clinical illnesses in horses. It analyses EHV-1 sequences obtained from equine abortion cases in Poland from 1999 to 2016, addressing its classification, distribution, and its role in epidemiological studies.

Research Methodology

  • The research was based on the characterization of EHV-1 sequences. These sequences were taken from cases of equine abortions that occurred in Poland between 1999 and 2016.
  • PCR (Polymerase chain reaction) products from open reading frames (ORF) 30 and 68 of the EHV-1 genome were sequenced.
  • A total of 38 EHV-1 sequences were obtained and examined.

Findings of the research

  • The researchers found out that the majority (about 82%) of the sequences did not belong to any of the previously described groups based on the ORF68 part of the genome.
  • The remaining sequences belonged either to ORF68 group III (8%) or to group IV (around 11%).
  • A haplotype network analysis did not reveal any clear structure within the networks of local Polish sequences, nor in a global network of 215 EHV-1 sequences.
  • The characteristics of these networks were not affected significantly by the geographical origin of the viruses or the date of detection.

Implications of the Research

  • The study suggests that ORF68 does not act as a reliable molecular marker in epidemiological studies of EHV-1 on a global scale.
  • The ability of ORF68 to aid local investigations of individual outbreaks is yet to be determined.
  • Most of the Polish EHV-1 sequences belonged to the ORF30 N genotype barring two sequences that belonged to the ORF30 D genotype.
  • The two sequences classified under the ORF30 D genotype were obtained during the years 2009 and 2010.
  • It was observed that the abortion cases that happened in Poland from 1999 to 2016 were predominantly caused by EHV-1 with the ORF30 N genotype.
  • The study did not indicate an increase in prevalence of the ORF30 D variant within that period.

Cite This Article

APA
Stasiak K, Dunowska M, Hills SF, Rola J. (2017). Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland. Arch Virol, 162(8), 2329-2335. https://doi.org/10.1007/s00705-017-3376-3

Publication

Archives of virology
ISSN: 1432-8798
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 162
Issue: 8
Pages: 2329-2335

Researcher Affiliations

Stasiak, Karol
  • Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100, Pulawy, Poland.
Dunowska, Magdalena
  • Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
Hills, Simon F
  • Institute of Agriculture and Environment, Massey University, Palmerston North, New Zealand.
Rola, Jerzy
  • Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100, Pulawy, Poland. jrola@piwet.pulawy.pl.

MeSH Terms

  • Abortion, Veterinary / epidemiology
  • Abortion, Veterinary / virology
  • Animals
  • Disease Outbreaks / veterinary
  • Female
  • Genotype
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Equid / genetics
  • Horse Diseases / diagnosis
  • Horse Diseases / virology
  • Horses / virology
  • Open Reading Frames
  • Poland / epidemiology
  • Polymerase Chain Reaction / veterinary
  • Pregnancy
  • Sequence Analysis, DNA

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 9 times.
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