Genetic diversity of Actinobacillus lignieresii isolates from different hosts.
Abstract: Genetic diversity detected by analysis of amplified fragment length polymorphisms (AFLPs) of 54 Actinobacillus lignieresii isolates from different hosts and geographic localities is described. On the basis of variances in AFLP profiles, the strains were grouped in two major clusters; one comprising strains isolated from horses and infected wounds of humans bitten by horses and another consisting of strains isolated from bovine and ovine hosts. The present data indicate a comparatively higher degree of genetic diversity among strains isolated from equine hosts and confirm the existence of a separate genomospecies for A. lignieresi-like isolates from horses. Among the isolates from bovine and ovine hosts some clonal lines appear to be genetically stable over time and could be detected at very distant geographic localities. Although all ovine strains investigated grouped in a single cluster, the existence of distinct genetic lineages that have evolved specificity for ovine hosts is not obvious and needs to be confirmed in other studies.
Publication Date: 2011-02-08 PubMed ID: 21303512PubMed Central: PMC3041693DOI: 10.1186/1751-0147-53-6Google Scholar: Lookup
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- Journal Article
Summary
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The study examines variations in genetic makeup of Actinobacillus lignieresii, a bacterium, found in different hosts and geographical locations. Two major groups were identified: one from horses and human horse-related wounds, and another from cows and sheep. Clearer differences were seen in isolates from horses.
Study Design and Results
- The study focused on analyzing the genetic diversity of Actinobacillus lignieresii, a type of bacterium, using amplified fragment length polymorphisms (AFLPs). AFLPs is a method that allows an in-depth analysis of the bacterium’s DNA, offering valuable insight into its genetic makeup.
- A total of 54 Actinobacillus lignieresii isolates were examined. These samples were obtained from varied sources including different hosts (like horses, cows, and sheep) and diverse geographical locations.
- Upon analysis, it was observed that the isolates could be grouped into two major clusters based on the variations in their AFLP profiles. One cluster consisted mainly of strains isolated from horses and infected wounds of humans bitten by horses. The other cluster was formed by strains obtained from bovine and ovine (sheep) hosts.
Further Observations
- The data suggested a relatively higher degree of genetic diversity among the strains that were isolated from equine hosts. This implies that the Actinobacillus lignieresii found in horses had more genetic variation than those found in bovine and sheep hosts.
- The study also confirmed the existence of a separate genomospecies for A. lignieresi-like isolates obtained from horses. A genomospecies is a type of bacteria that can be defined based on its genetic constitution rather than its physiological characteristics.
- Among the strains isolated from bovine and ovine hosts, some appeared to be genetically stable over time and could be detected at very distant geographical locations. This indicates a genetic consistency and adaptability in these strains of A. lignieresii despite the geographical differences.
- However, despite all of the ovine strains grouping into a single cluster, the existence of distinct genetic lineages that have evolved specifically for ovine hosts is still uncertain and requires further research.
Cite This Article
APA
Kokotovic B, Angen Ø, Bisgaard M.
(2011).
Genetic diversity of Actinobacillus lignieresii isolates from different hosts.
Acta Vet Scand, 53(1), 6.
https://doi.org/10.1186/1751-0147-53-6 Publication
Researcher Affiliations
- Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. bkok@vet.dtu.dk
MeSH Terms
- Actinobacillus / classification
- Actinobacillus / genetics
- Animals
- Cattle / microbiology
- Genetic Variation
- Horses / microbiology
- Phylogeny
- Sheep / microbiology
References
This article includes 14 references
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