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Home / Equine Research Bank / Parasit Vectors / Genetic diversity of common Gasterophilus spp. from distinct...
Parasites & vectors2018; 11(1); 474; doi: 10.1186/s13071-018-3042-y

Genetic diversity of common Gasterophilus spp. from distinct habitats in China.

Abstract: Gasterophilus species are widely distributed around the world. The larvae of these flies parasitize the digestive tract of equids and cause damage, hindering horse breeding and protection of endangered species. However, study of the genetic structure of geographically distinct Gasterophilus populations is lacking. Here, we analyzed the genetic diversity of Gasterophilus pecorum, G. intestinalis, G. nasalis and G. nigricornis from three typical grasslands (meadow, desert and alpine steppes) in China as compared to published sequences from Italy, Poland and China (Daqing and Yili), based on the mitochondrial cytochrome c oxidase cox1 and cox2 gene sequences. Results: Haplotype diversity and nucleotide diversity of mitochondrial genes was generally high in all Gasterophilus populations. Due to the unique natural climatic conditions of the alpine steppe, there were high levels of genetic differentiation among different geographical populations of G. pecorum and G. nasalis, indicating that environmental variations influenced population genetic structure. Frequent exchanges between meadow and desert steppe Gasterophilus species resulted in low genetic differentiation. The highest exchange rates were found among G. intestinalis populations. Genetic differentiation was only observed on a large geographical scale, which was confirmed by analyzing population genetic structure. Three species, G. pecorum, G. intestinalis and G. nasalis, from meadow steppe showed a high emigration rate, indicating that the direction of Gasterophilus dispersal in China was from east to west. Conclusions: Our results show that the four Gasterophilus species have a high level of genetic diversity and different degrees of genetic differentiation and gene flow among different populations of the same species, reflecting their potential to adapt to the environment and the environmental impact on genetic structure. Knowledge of the genetic structure, population history, and migration will help understand the occurrence and prevalence of gasterophilosis and provide a basis for controlling the local spread of Gasterophilus spp.
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Publication Date: 2018-08-22 PubMed ID: 30134994PubMed Central: PMC6106871DOI: 10.1186/s13071-018-3042-yGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study delves into the genetic diversity of certain species of Gasterophilus, a breed of flies, across different habitat types in China. By analyzing the genetic structure of the flies, conclusions were drawn on their adaptability and migration patterns, which in turn provides insight on how to better control their spread.

Research Method

  • The researchers chose four species of Gasterophilus: G. pecorum, G. intestinalis, G. nasalis, and G. nigricornis.
  • These species were collected from three different grassland types in China – meadow, desert, and alpine steppes.
  • To understand their genetic diversity, the researchers analyzed two mitochondrial genes: cytochrome c oxidase cox1 and cox2.
  • The analysis was compared with published sequences from Italy, Poland and China (Daqing and Yili) to provide global context.

Findings

  • Generally, every Gasterophilus population exhibited high genetic diversity.
  • Due to unique climatic conditions in the alpine steppe, the species G. pecorum and G. nasalis showed high levels of genetic differentiation among different geographical populations.
  • There was frequent gene exchange between Gasterophilus species in the meadow and desert steppe, resulting in low genetic differentiation.
  • The genetic differentiation was only observed over large geographical scales.
  • There was more gene exchange noted among G. intestinalis populations than the other species.
  • Three species from the meadow steppe, G. pecorum, G. intestinalis, and G. nasalis showed a high emigration rate, indicating that these flies migrate from east to west in China.

Conclusions

  • Distinct levels of genetic diversity, differentiation, and gene flow were found among different populations of the four Gasterophilus species.
  • This diversity and differentiation reflect the species’ adaptability to changing environments and how environmental factors impact their genetic structure.
  • Understanding the genetic structure, population history, and migration of these species could provide valuable information on how to control the spread of Gasterophilus species.

These findings offer unique insights into how genetic structure, diversity, and migration of these parasitic flies are influenced by their environment, giving valuable information on their control and prevention within different habitats.

Cite This Article

APA
Zhang B, Huang H, Wang H, Zhang D, Chu H, Ma X, Ge Y, Ente M, Li K. (2018). Genetic diversity of common Gasterophilus spp. from distinct habitats in China. Parasit Vectors, 11(1), 474. https://doi.org/10.1186/s13071-018-3042-y

Publication

Parasites & vectors
ISSN: 1756-3305
NlmUniqueID: 101462774
Country: England
Language: English
Volume: 11
Issue: 1
Pages: 474
PII: 474

Researcher Affiliations

Zhang, Boru
  • Key Laboratory of Non-Invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, Beijing, 100083, China.
  • Qinhuangdao Forestry Bureau, Qinhuangdao, 066004, Hebei, China.
Huang, Heqing
  • Key Laboratory of Non-Invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, Beijing, 100083, China.
Wang, Haoyu
  • Key Laboratory of Non-Invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, Beijing, 100083, China.
Zhang, Dong
  • Key Laboratory of Non-Invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, Beijing, 100083, China.
Chu, Hongjun
  • Wildlife Conservation Office of Altay Prefecture, Altay, 836599, Xinjiang, China.
Ma, Xinping
  • Xinjiang Research Centre for Breeding Przewalski's Horse, Urumqi, 831700, Xinjiang, China.
Ge, Yan
  • Wildlife Conservation Office of Altay Prefecture, Altay, 836599, Xinjiang, China.
Ente, Make
  • Xinjiang Research Centre for Breeding Przewalski's Horse, Urumqi, 831700, Xinjiang, China.
Li, Kai
  • Key Laboratory of Non-Invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, Beijing, 100083, China. likai_sino@sina.com.

MeSH Terms

  • Animal Distribution
  • Animal Migration
  • Animals
  • China / epidemiology
  • DNA, Mitochondrial / genetics
  • Diptera / genetics
  • Ecosystem
  • Ectoparasitic Infestations / epidemiology
  • Ectoparasitic Infestations / veterinary
  • Gastrointestinal Tract / parasitology
  • Gene Flow
  • Genetic Variation
  • Genetics, Population
  • Haplotypes
  • Horse Diseases / epidemiology
  • Horse Diseases / parasitology
  • Horses
  • Italy / epidemiology
  • Poland / epidemiology
  • Sequence Analysis, DNA

Grant Funding

  • 31670538 / National Science Foundation of China
  • JC2015-04 / Fundamental Research Funds for the Central Universities
  • 201610022073 / Student's Platform for Innovation and Entrepreneurship Training Program
  • 2015-123 / Project of Department for Wildlife and Forest Plants Protection, SFA of China

Conflict of Interest Statement

ETHICS APPROVAL: The study was performed in accordance with the relevant guidelines and regulations regarding animal welfare. All experimental protocols were approved by Wildlife Conservation Office of Altay Prefecture and Beijing Forestry University. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

This article includes 39 references
  1. Zumpt F. Myiasis in man and animals in the old world: a textbook for physicians, veterinarians and zoologists.. London: Butterworths; 1965.
  2. Fan ZD. Index of common flies in China.. Beijing: Science Press; 1992.
  3. Wang MF. Flies in China (Vol. II). Shenyang: Science and Technology Press; 1998.
  4. Li K, Wu Z, Hu DF, Cao J, Wang C. A report on new causative agent (Gasterophilus spp.) of the myiasis of Przewalski’s horse occurred in China.. Acta Vet Et Zootech Sin 2007;38:837–840.
  5. Hall M, Wall R. Myiasis of humans and domestic animals.. Adv Parasitol 1995;35:257-334.
    doi: 10.1016/S0065-308X(08)60073-1pubmed: 7709854google scholar: lookup
  6. Sequeira JL, Tostes RA, Oliveira-Sequeira TC. Prevalence and macro- and microscopic lesions produced by Gasterophilus nasalis (Diptera: Oestridae) in the Botucatu Region, SP, Brazil.. Vet Parasitol 2001 Dec 13;102(3):261-6.
    doi: 10.1016/S0304-4017(01)00536-2pubmed: 11777606google scholar: lookup
  7. Sánchez-Andrade R, Cortiñas FJ, Francisco I, Sánchez JA, Mula P, Cazapal C, Vázquez L, Suárez JL, Francisco R, Arias MS, Díez-Baños P, Scala A, Paz-Silva A. A novel second instar Gasterophilus excretory/secretory antigen-based ELISA for the diagnosis of gasterophilosis in grazing horses.. Vet Parasitol 2010 Aug 4;171(3-4):314-20.
    doi: 10.1016/j.vetpar.2010.03.034pubmed: 20430529google scholar: lookup
  8. Edwards GT. The prevalence of Gasterophilus intestinalis in horses in northern England and Wales.. Vet Parasitol 1982 Nov;11(2-3):215-22.
    pubmed: 6891853doi: 10.1016/0304-4017(82)90044-9google scholar: lookup
  9. Hatch C, McCaughey WJ, O'Brien JJ. The prevalence of Gasterophilus intestinalis and G nasalis in horses in Ireland.. Vet Rec 1976 Apr 3;98(14):274-6.
    doi: 10.1136/vr.98.14.274pubmed: 1274134google scholar: lookup
  10. Lyons ET, Swerczek TW, Tolliver SC, Bair HD, Drudge JH, Ennis LE. Prevalence of selected species of internal parasites in equids at necropsy in central Kentucky (1995-1999).. Vet Parasitol 2000 Sep 10;92(1):51-62.
    doi: 10.1016/S0304-4017(00)00266-1pubmed: 10936545google scholar: lookup
  11. Otranto D, Milillo P, Capelli G, Colwell DD. Species composition of Gasterophilus spp. (Diptera, Oestridae) causing equine gastric myiasis in southern Italy: parasite biodiversity and risks for extinction.. Vet Parasitol 2005 Oct 10;133(1):111-8.
    doi: 10.1016/j.vetpar.2005.05.015pubmed: 15978726google scholar: lookup
  12. Niedźwiedź A, Borowicz H, Nicpoń JM. Prevalence study in horses infected by Gasterophilus sp. in an eastern region of Poland.. Vet Parasitol 2013 Jan 16;191(1-2):94-6.
    doi: 10.1016/j.vetpar.2012.08.023pubmed: 22998809google scholar: lookup
  13. Pilo C, Altea A, Scala A. Gasterophilosis in horses in Sardinia (Italy): effect of meteorological variables on adult egg-laying activity and presence of larvae in the digestive tract, and update of species.. Parasitol Res 2015 May;114(5):1693-702.
    doi: 10.1007/s00436-015-4352-zpubmed: 25663068google scholar: lookup
  14. Wang W, Xiao S, Huang H, Li K, Zhang D, Chu H. Diversity and infection of Gasterophilus spp. in Mongol- Xinjiang Region and Qinghai Tibet Region.. Sci Silvae Sin 2016;52:134–139.
  15. Fresia P, Azeredo-Espin AM, Lyra ML. The phylogeographic history of the new world screwworm fly, inferred by approximate bayesian computation analysis.. PLoS One 2013;8(10):e76168.
    doi: 10.1371/journal.pone.0076168pmc: PMC3788763pubmed: 24098436google scholar: lookup
  16. Ståhls G, Vujić A, Petanidou T, Cardoso P, Radenković S, Ačanski J, Pérez Bañón C, Rojo S. Phylogeographic patterns of Merodon hoverflies in the Eastern Mediterranean region: revealing connections and barriers.. Ecol Evol 2016 Apr;6(7):2226-45.
    doi: 10.1002/ece3.2021pmc: PMC4782255pubmed: 27069578google scholar: lookup
  17. Dincă V, Runquist M, Nilsson M, Vila R. Dispersal, fragmentation, and isolation shape the phylogeography of the European lineages of Polyommatus (Agrodiaetus) ripartii (Lepidoptera: Lycaenidae). Biol J Linn Soc 2013;109:817–829.
    doi: 10.1111/bij.12096google scholar: lookup
  18. Miranda EA, Batalha-Filho H, Congrains C, Carvalho AF, Ferreira KM, Del Lama MA. Phylogeography of Partamona rustica (Hymenoptera, Apidae), an Endemic Stingless Bee from the Neotropical Dry Forest Diagonal.. PLoS One 2016;11(10):e0164441.
    doi: 10.1371/journal.pone.0164441pmc: PMC5056711pubmed: 27723778google scholar: lookup
  19. Ye Z, Zhu G, Damgaard J, Chen X, Chen P, Bu W. Phylogeography of a semi-aquatic bug, Microvelia horvathi (Hemiptera: Veliidae): an evaluation of historical, geographical and ecological factors.. Sci Rep 2016 Feb 29;6:21932.
    doi: 10.1038/srep21932pmc: PMC4770413pubmed: 26923804google scholar: lookup
  20. Pawlas-Opiela M, Wojciech Ł, Sołtysiak Z, Otranto D, Ugorski M. Molecular comparison of Gasterophilus intestinalis and Gasterophilus nasalis from two distinct areas of Poland and Italy based on cox1 sequence analysis.. Vet Parasitol 2010 Apr 19;169(1-2):219-21.
    doi: 10.1016/j.vetpar.2009.12.030pubmed: 20080351google scholar: lookup
  21. Gao DZ, Liu GH, Wang GL, Zhu XQ, Wang CR. Sequence variation in three mitochondrial genes among Gasterophilus intestinalis isolates from two distinct regions in China.. Mitochondrial DNA A DNA Mapp Seq Anal 2017 Jan;28(1):37-40.
    pubmed: 26710301doi: 10.3109/19401736.2015.1106529google scholar: lookup
  22. Chu HJ, Jiang ZG, Ge Y, Jiang F, Tao Y, Wang C. Population densities and number of khulan and goitred gazelle in Mt. Kalamaili Ungulate Nature Reserve.. Biodiversity Sci 2009;17:414–422.
    doi: 10.3724/SP.J.1003.2009.09001google scholar: lookup
  23. Aruhan YC. Analysis on the characteristics of climate change in recent 50 years in Duolun County, Inner Mongolia.. J Inner Mongolia Univ 2007;38:434–438.
  24. Cheng TH, Chang YT. On tentative scheme for dividing zoogeographical regions of China.. Acta Geogr Sin 1956;1:2.
  25. Zhang RZ. Zoogeography of China.. Beijing: Science Press; 1999.
  26. Han Y, Li G. Climate change of Yellow River source region for nearly 30 years in Maduo County.. J Qinghai Meteor 2011;2:36–41.
  27. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3.. New York: Cold Spring Harbor Laboratory Press; 2001.
  28. Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers.. Ann Entomol Soc Am 1994;87:651–701.
    doi: 10.1093/aesa/87.6.651google scholar: lookup
  29. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.. Nucleic Acids Symp Ser 1999;41:95–98.
  30. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.. Mol Biol Evol 2016 Jul;33(7):1870-4.
    doi: 10.1093/molbev/msw054pmc: PMC8210823pubmed: 27004904google scholar: lookup
  31. Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data.. Bioinformatics 2009 Jun 1;25(11):1451-2.
    doi: 10.1093/bioinformatics/btp187pubmed: 19346325google scholar: lookup
  32. Felsenstein J. CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.. Evolution 1985 Jul;39(4):783-791.
    doi: 10.1111/j.1558-5646.1985.tb00420.xpubmed: 28561359google scholar: lookup
  33. Clement M, Posada D, Crandall KA. TCS: a computer program to estimate gene genealogies.. Mol Ecol 2000 Oct;9(10):1657-9.
    doi: 10.1046/j.1365-294x.2000.01020.xpubmed: 11050560google scholar: lookup
  34. Kuhner MK. LAMARC 2.0: maximum likelihood and Bayesian estimation of population parameters.. Bioinformatics 2006 Mar 15;22(6):768-70.
    doi: 10.1093/bioinformatics/btk051pubmed: 16410317google scholar: lookup
  35. Wang W, Zhang D, Hu D, Chu H, Cao J, Ente M, Jiang G, Li K. Population genetic structure of Gasterophilus pecorum in the Kalamaili Nature Reserve, Xinjiang, based on mitochondrial cytochrome oxidase (COI) gene sequence.. Med Vet Entomol 2014 Aug;28 Suppl 1:75-82.
    doi: 10.1111/mve.12073pubmed: 25171609google scholar: lookup
  36. Morgan ER, Clare EL, Jefferies R, Stevens JR. Parasite epidemiology in a changing world: can molecular phylogeography help us tell the wood from the trees?. Parasitology 2012 Dec;139(14):1924-38.
    doi: 10.1017/S0031182012001060pubmed: 22917112google scholar: lookup
  37. McCoy K, Beis P, Barbosa A, Cuervo JJ, Fraser WR, González-Solís J. Population genetic structure and colonisation of the western Antarctic Peninsula by the seabird tick Ixodes uriae.. Mar Eco Prog Ser 2012;459:109–120.
    doi: 10.3354/meps09749google scholar: lookup
  38. Criscione CD, Poulin R, Blouin MS. Molecular ecology of parasites: elucidating ecological and microevolutionary processes.. Mol Ecol 2005 Jul;14(8):2247-57.
    doi: 10.1111/j.1365-294X.2005.02587.xpubmed: 15969711google scholar: lookup
  39. Liu X. The Silk Road in world history.. New York: Oxford University Press; 2010.

Citations

This article has been cited 3 times.
  1. Ramilo A, Rodríguez H, Pascual S, González ÁF, Abollo E. Population Genetic Structure of Anisakis simplex Infecting the European Hake from North East Atlantic Fishing Grounds.. Animals (Basel) 2023 Jan 4;13(2).
    doi: 10.3390/ani13020197pubmed: 36670737google scholar: lookup
  2. Huang H, Zhang K, Shao C, Wang C, Ente M, Wang Z, Zhang D, Li K. Spatial distribution of Gasterophilus pecorum (Diptera) eggs in the desert steppe of the Kalamaili Nature Reserve (Xinjiang, China).. BMC Ecol Evol 2021 Sep 6;21(1):169.
    doi: 10.1186/s12862-021-01897-4pubmed: 34488639google scholar: lookup
  3. Zhang K, Huang H, Zhou R, Zhang B, Wang C, Ente M, Li B, Zhang D, Li K. The impact of temperature on the life cycle of Gasterophilus pecorum in northwest China.. Parasit Vectors 2021 Mar 1;14(1):129.
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