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Parasitology research2015; 114(4); 1619-1624; doi: 10.1007/s00436-015-4353-y

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland.

Abstract: Faecal samples were collected from 352 horses on 23 farms operating under six different management systems in the Czech Republic and Poland during 2011 and 2012. Farms were selected without previous knowledge of parasitological status. All faecal samples were screened for Cryptosporidium spp. presence using microscopy, following aniline-carbol-methyl violet staining and PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes. Cryptosporidium muris-positive samples were additionally genotyped at four minisatellite markers: MS1 (encoding a hypothetical protein), MS2 (encoding a 90-kDa heat shock protein), MS3 (encoding a hypothetical protein) and MS16 (encoding a leucine-rich repeat family protein). Cryptosporidium spp. was detected by PCR in 12/352 (3.4%) samples from 4 out of 13 farms. None of the samples tested by microscopy was positive. There was no relationship between Cryptosporidium prevalence and age, sex, diarrhoea or management system; however, Cryptosporidium was found only on farms where horses were kept on pasture during the day and in a stable overnight. Sequence analyses of SSU and gp60 genes revealed the presence of C. muris RN66 (n = 9), Cryptosporidium parvum IIaA15G2R1 (n = 1), Cryptosporidium tyzzeri IXbA22R9 (n = 1), and Cryptosporidium horse genotype VIaA15G4 (n = 1). The C. muris subtypes were identified as MS1-M1, MS2-M4, novel MS2-M7 and MS16-M1 by multilocus sequence of three minisatellite loci. The MS3 locus was not amplified from any isolate. This is the first report of C. tyzzeri and C. muris subtypes from horses.
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Publication Date: 2015-02-27 PubMed ID: 25722018DOI: 10.1007/s00436-015-4353-yGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study delves into the genetic diversity of Cryptosporidium species present in horses in Czech Republic and Poland. It reports a novel identification of two species, Cryptosporidium muris and Cryptosporidium tyzzeri in horses, that were previously not reported.

Research Methodology

  • For this research, faecal samples were collected from a total of 352 horses. These horses belonged to 23 different farms operating under six distinct management systems in the Czech Republic and Poland, during the years 2011 and 2012.
  • The researchers performed their selection of farms without any prior knowledge of the parasitological status of these facilities.
  • All collected faecal samples were thoroughly screened for the presence of Cryptosporidium spp., utilizing microscopy after staining with aniline-carbol-methyl violet and performing PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes.

Research Findings

  • The analysis found that out of the 352 samples, Cryptosporidium spp. was detected in 12 samples (3.4%) from 4 out of the 13 farms.
  • Interestingly, none of the samples tested positive for Cryptosporidium when tested under microscopy.
  • The study also found that the occurrence of Cryptosporidium had no relationship with age, sex, presence of diarrhoea, or the management system of the farm. However, the parasitic species were only found on farms where horses were kept on pasture during the day and in a stable overnight.
  • Sequence analyses of SSU and gp60 genes starkly revealed the presence of C. muris RN66, Cryptosporidium parvum IIaA15G2R1, Cryptosporidium tyzzeri IXbA22R9, and Cryptosporidium horse genotype VIaA15G4 in the positive samples.
  • Additionally, the C. muris subtypes were further identified as MS1-M1, MS2-M4, a novel subtype MS2-M7, and MS16-M1 by a multilocus sequence of three minisatellite loci.
  • However, the MS3 locus could not be amplified from any isolate.

Significance of the Study

  • This study has important implications as it reports for the first time the presence of C. tyzzeri and C. muris subtypes in horses, indicating a wider species variation of Cryptosporidium spp. in horses than previously known.
  • These findings contribute to the body of knowledge about Cryptosporidium subspecies and their prevalence in equine hosts, which can have implications in understanding their transmission and effect on horse health.

Cite This Article

APA
Wagnerová P, Sak B, McEvoy J, Rost M, Matysiak AP, Ježková J, Kváč M. (2015). Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland. Parasitol Res, 114(4), 1619-1624. https://doi.org/10.1007/s00436-015-4353-y

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 114
Issue: 4
Pages: 1619-1624

Researcher Affiliations

Wagnerová, Pavla
  • Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 13, 370 05, České Budějovice, Czech Republic.
Sak, Bohumil
    McEvoy, John
      Rost, Michael
        Matysiak, Agniezska Perec
          Ježková, Jana
            Kváč, Martin

              MeSH Terms

              • Animals
              • Cryptosporidiosis / epidemiology
              • Cryptosporidium / classification
              • Cryptosporidium / genetics
              • Cryptosporidium / isolation & purification
              • Czech Republic / epidemiology
              • Diarrhea / parasitology
              • Diarrhea / veterinary
              • Feces / parasitology
              • Female
              • Genetic Variation
              • Genotype
              • Horse Diseases / parasitology
              • Horses
              • Male
              • Poland / epidemiology
              • Prevalence

              References

              This article includes 34 references
              1. Grinberg A, Learmonth J, Kwan E, Pomroy W, Lopez Villalobos N, Gibson I, Widmer G. Genetic diversity and zoonotic potential of Cryptosporidium parvum causing foal diarrhea.. J Clin Microbiol 2008 Jul;46(7):2396-8.
                pubmed: 18508944doi: 10.1128/JCM.00936-08google scholar: lookup
              2. Sulaiman IM, Hira PR, Zhou L, Al-Ali FM, Al-Shelahi FA, Shweiki HM, Iqbal J, Khalid N, Xiao L. Unique endemicity of cryptosporidiosis in children in Kuwait.. J Clin Microbiol 2005 Jun;43(6):2805-9.
                pubmed: 15956401doi: 10.1128/JCM.43.6.2805-2809.2005google scholar: lookup
              3. Wielinga PR, de Vries A, van der Goot TH, Mank T, Mars MH, Kortbeek LM, van der Giessen JW. Molecular epidemiology of Cryptosporidium in humans and cattle in The Netherlands.. Int J Parasitol 2008 Jun;38(7):809-17.
                pubmed: 18054936doi: 10.1016/j.ijpara.2007.10.014google scholar: lookup
              4. Xiao L, Herd RP. Epidemiology of equine Cryptosporidium and Giardia infections.. Equine Vet J 1994 Jan;26(1):14-7.
                pubmed: 8143656doi: 10.1111/j.2042-3306.1994.tb04323.xgoogle scholar: lookup
              5. Feng Y, Yang W, Ryan U, Zhang L, Kvác M, Koudela B, Modry D, Li N, Fayer R, Xiao L. Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni.. J Clin Microbiol 2011 Jan;49(1):34-41.
                pubmed: 20980577doi: 10.1128/JCM.01329-10google scholar: lookup
              6. Grinberg A, Oliver L, Learmonth JJ, Leyland M, Roe W, Pomroy WE. Identification of Cryptosporidium parvum 'cattle' genotype from a severe outbreak of neonatal foal diarrhoea.. Vet Rec 2003 Nov 15;153(20):628-31.
                pubmed: 14653344doi: 10.1136/vr.153.20.628google scholar: lookup
              7. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.. Mol Biol Evol 2011 Oct;28(10):2731-9.
                pubmed: 21546353doi: 10.1093/molbev/msr121google scholar: lookup
              8. Perrucci S, Buggiani C, Sgorbini M, Cerchiai I, Otranto D, Traversa D. Cryptosporidium parvum infection in a mare and her foal with foal heat diarrhoea.. Vet Parasitol 2011 Dec 15;182(2-4):333-6.
                pubmed: 21715096doi: 10.1016/j.vetpar.2011.05.051google scholar: lookup
              9. Wang R, Jian F, Zhang L, Ning C, Liu A, Zhao J, Feng Y, Qi M, Wang H, Lv C, Zhao G, Xiao L. Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.. PLoS One 2012;7(8):e43782.
                pubmed: 22937094doi: 10.1371/journal.pone.0043782google scholar: lookup
              10. Maddox-Hyttel C, Langkjaer RB, Enemark HL, Vigre H. Cryptosporidium and Giardia in different age groups of Danish cattle and pigs--occurrence and management associated risk factors.. Vet Parasitol 2006 Oct 10;141(1-2):48-59.
                pubmed: 16797848doi: 10.1016/j.vetpar.2006.04.032google scholar: lookup
              11. Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal.. J Clin Microbiol 2003 Jun;41(6):2744-7.
                pubmed: 12791920doi: 10.1128/JCM.41.6.2744-2747.2003google scholar: lookup
              12. Glaberman S, Moore JE, Lowery CJ, Chalmers RM, Sulaiman I, Elwin K, Rooney PJ, Millar BC, Dooley JS, Lal AA, Xiao L. Three drinking-water-associated cryptosporidiosis outbreaks, Northern Ireland.. Emerg Infect Dis 2002 Jun;8(6):631-3.
                pubmed: 12023922doi: 10.3201/eid0806.010368google scholar: lookup
              13. Olson ME, Thorlakson CL, Deselliers L, Morck DW, McAllister TA. Giardia and Cryptosporidium in Canadian farm animals.. Vet Parasitol 1997 Mar;68(4):375-81.
                pubmed: 9106959doi: 10.1016/s0304-4017(96)01072-2google scholar: lookup
              14. Ramirez NE, Ward LA, Sreevatsan S. A review of the biology and epidemiology of cryptosporidiosis in humans and animals.. Microbes Infect 2004 Jul;6(8):773-85.
                pubmed: 15207825doi: 10.1016/j.micinf.2004.02.021google scholar: lookup
              15. Snyder SP, England JJ, McChesney AE. Cryptosporidiosis in immunodeficient Arabian foals.. Vet Pathol 1978 Jan;15(1):12-7.
                pubmed: 625861doi: 10.1177/030098587801500102google scholar: lookup
              16. Veronesi F, Passamonti F, Cacciò S, Diaferia M, Piergili Fioretti D. Epidemiological survey on equine cryptosporidium and giardia infections in Italy and molecular characterization of isolates.. Zoonoses Public Health 2010 Dec;57(7-8):510-7.
                pubmed: 19912609doi: 10.1111/j.1863-2378.2009.01261.xgoogle scholar: lookup
              17. Kváč M, Saková K, Kvĕtoňová D, Kicia M, Wesołowska M, McEvoy J, Sak B. Gastroenteritis caused by the Cryptosporidium hedgehog genotype in an immunocompetent man.. J Clin Microbiol 2014 Jan;52(1):347-9.
                pubmed: 24131692doi: 10.1128/JCM.02456-13google scholar: lookup
              18. Majewska AC, Solarczyk P, Tamang L, Graczyk TK. Equine Cryptosporidium parvum infections in western Poland.. Parasitol Res 2004 Jul;93(4):274-8.
                pubmed: 15156396doi: 10.1007/s00436-004-1111-ygoogle scholar: lookup
              19. Jiang J, Alderisio KA, Xiao L. Distribution of cryptosporidium genotypes in storm event water samples from three watersheds in New York.. Appl Environ Microbiol 2005 Aug;71(8):4446-54.
                pubmed: 16085835doi: 10.1128/AEM.71.8.4446-4454.2005google scholar: lookup
              20. Burton AJ, Nydam DV, Dearen TK, Mitchell K, Bowman DD, Xiao L. The prevalence of Cryptosporidium, and identification of the Cryptosporidium horse genotype in foals in New York State.. Vet Parasitol 2010 Nov 24;174(1-2):139-44.
                pubmed: 20932647doi: 10.1016/j.vetpar.2010.08.019google scholar: lookup
              21. Gajadhar AA, Caron JP, Allen JR. Cryptosporidiosis in two foals.. Can Vet J 1985 Apr;26(4):132-4.
                pubmed: 17422522
              22. Trotz-Williams LA, Martin DS, Gatei W, Cama V, Peregrine AS, Martin SW, Nydam DV, Jamieson F, Xiao L. Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario.. Parasitol Res 2006 Sep;99(4):346-52.
                pubmed: 16565813doi: 10.1007/s00436-006-0157-4google scholar: lookup
              23. Milácek P, Vítovec J. Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from feces and scrapings of intestinal mucosa.. Folia Parasitol (Praha) 1985;32(1):50.
                pubmed: 2580763
              24. Caffara M, Piva S, Pallaver F, Iacono E, Galuppi R. Molecular characterization of Cryptosporidium spp. from foals in Italy.. Vet J 2013 Nov;198(2):531-3.
                pubmed: 24138936doi: 10.1016/j.tvjl.2013.09.004google scholar: lookup
              25. De Souza PN, Bomfim TC, Huber F, Abboud LC, Gomes RS. Natural infection by Cryptosporidium sp., Giardia sp. and Eimeria leuckarti in three groups of equines with different handlings in Rio de Janeiro, Brazil.. Vet Parasitol 2009 Mar 23;160(3-4):327-33.
                pubmed: 19117684doi: 10.1016/j.vetpar.2008.10.103google scholar: lookup
              26. Mohammed HO, Wade SE, Schaaf S. Risk factors associated with Cryptosporidium parvum infection in dairy cattle in southeastern New York State.. Vet Parasitol 1999 Jun 1;83(1):1-13.
                pubmed: 10392763doi: 10.1016/s0304-4017(99)00032-1google scholar: lookup
              27. Laatamna AE, Wagnerová P, Sak B, Květoňová D, Aissi M, Rost M, Kváč M. Equine cryptosporidial infection associated with Cryptosporidium hedgehog genotype in Algeria.. Vet Parasitol 2013 Oct 18;197(1-2):350-3.
                pubmed: 23731858doi: 10.1016/j.vetpar.2013.04.041google scholar: lookup
              28. McKenzie DM, Diffay BC. Diarrhoea associated with cryptosporidial oocyst shedding in a quarterhorse stallion.. Aust Vet J 2000 Jan;78(1):27-8.
                pubmed: 10736680doi: 10.1111/j.1751-0813.2000.tb10351.xgoogle scholar: lookup
              29. Grinberg A, Pomroy WE, Carslake HB, Shi Y, Gibson IR, Drayton BM. A study of neonatal cryptosporidiosis of foals in New Zealand.. N Z Vet J 2009 Oct;57(5):284-9.
                pubmed: 19802042doi: 10.1080/00480169.2009.58622google scholar: lookup
              30. Xiao L, Hlavsa MC, Yoder J, Ewers C, Dearen T, Yang W, Nett R, Harris S, Brend SM, Harris M, Onischuk L, Valderrama AL, Cosgrove S, Xavier K, Hall N, Romero S, Young S, Johnston SP, Arrowood M, Roy S, Beach MJ. Subtype analysis of Cryptosporidium specimens from sporadic cases in Colorado, Idaho, New Mexico, and Iowa in 2007: widespread occurrence of one Cryptosporidium hominis subtype and case history of an infection with the Cryptosporidium horse genotype.. J Clin Microbiol 2009 Sep;47(9):3017-20.
                pubmed: 19587303doi: 10.1128/JCM.00226-09google scholar: lookup
              31. Kváč M, McEvoy J, Loudová M, Stenger B, Sak B, Květoňová D, Ditrich O, Rašková V, Moriarty E, Rost M, Macholán M, Piálek J. Coevolution of Cryptosporidium tyzzeri and the house mouse (Mus musculus).. Int J Parasitol 2013 Sep;43(10):805-17.
                pubmed: 23791796doi: 10.1016/j.ijpara.2013.04.007google scholar: lookup
              32. Cole DJ, Cohen ND, Snowden K, Smith R. Prevalence of and risk factors for fecal shedding of Cryptosporidium parvum oocysts in horses.. J Am Vet Med Assoc 1998 Nov 1;213(9):1296-302.
                pubmed: 9810386
              33. Sturdee AP, Bodley-Tickell AT, Archer A, Chalmers RM. Long-term study of Cryptosporidium prevalence on a lowland farm in the United Kingdom.. Vet Parasitol 2003 Oct 8;116(2):97-113.
                pubmed: 14519315doi: 10.1016/s0304-4017(03)00261-9google scholar: lookup
              34. Ryan U, Xiao L, Read C, Zhou L, Lal AA, Pavlasek I. Identification of novel Cryptosporidium genotypes from the Czech Republic.. Appl Environ Microbiol 2003 Jul;69(7):4302-7.
                pubmed: 12839819doi: 10.1128/AEM.69.7.4302-4307.2003google scholar: lookup

              Citations

              This article has been cited 19 times.
              1. Li XM, Geng HL, Wei YJ, Yan WL, Liu J, Wei XY, Zhang M, Wang XY, Zhang XX, Liu G. Global prevalence and risk factors of Cryptosporidium infection in Equus: A systematic review and meta-analysis. Front Cell Infect Microbiol 2022;12:1072385.
                doi: 10.3389/fcimb.2022.1072385pubmed: 36506009google scholar: lookup
              2. Ryan U, Zahedi A, Feng Y, Xiao L. An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans. Animals (Basel) 2021 Nov 19;11(11).
                doi: 10.3390/ani11113307pubmed: 34828043google scholar: lookup
              3. Mathison BA, Sapp SGH. An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae. Zookeys 2021;1069:1-313.
                doi: 10.3897/zookeys.1069.67403pubmed: 34819766google scholar: lookup
              4. Wang L, Cao L, Zheng S, Chang Y, Zhang K, Zhang S, Zhang L. Molecular identification and biological characterization of Cryptosporidium muris from camels (Camelus bactrianus) in China. Parasit Vectors 2021 Jul 15;14(1):365.
                doi: 10.1186/s13071-021-04862-8pubmed: 34266490google scholar: lookup
              5. Arias-Agudelo LM, Garcia-Montoya G, Cabarcas F, Galvan-Diaz AL, Alzate JF. Comparative genomic analysis of the principal Cryptosporidium species that infect humans. PeerJ 2020;8:e10478.
                doi: 10.7717/peerj.10478pubmed: 33344091google scholar: lookup
              6. García-Livia K, Martín-Alonso A, Foronda P. Diversity of Cryptosporidium spp. in wild rodents from the Canary Islands, Spain. Parasit Vectors 2020 Sep 4;13(1):445.
                doi: 10.1186/s13071-020-04330-9pubmed: 32887646google scholar: lookup
              7. Wang W, Zhang Z, Zhang Y, Zhao A, Jing B, Zhang L, Liu P, Qi M, Zhao W. Prevalence and genotypic identification of Cryptosporidium in free-ranging and farm-raised donkeys (Equus asinus asinus) in Xinjiang, China. Parasite 2020;27:45.
                doi: 10.1051/parasite/2020042pubmed: 32584252google scholar: lookup
              8. Couso-Pérez S, de Limia FB, Ares-Mazás E, Gómez-Couso H. First report of zoonotic Cryptosporidium parvum GP60 subtypes IIaA15G2R1 and IIaA16G3R1 in wild ponies from the northern Iberian Peninsula. Parasitol Res 2020 Jan;119(1):249-254.
                doi: 10.1007/s00436-019-06529-xpubmed: 31758296google scholar: lookup
              9. Huang X, Zhou Z, Liu H, Deng L, Bi B, Chai Y, Zhong Z, Hu Y, Fu H, Peng G. New genotypes and molecular characterization of Enterocytozoon bieneusi in captive black bears in China. Int J Parasitol Parasites Wildl 2019 Dec;10:1-5.
                doi: 10.1016/j.ijppaw.2019.06.012pubmed: 31316895google scholar: lookup
              10. Plutzer J, Lassen B, Jokelainen P, Djurković-Djaković O, Kucsera I, Dorbek-Kolin E, Šoba B, Sréter T, Imre K, Omeragić J, Nikolić A, Bobić B, Živičnjak T, Lučinger S, Stefanović LL, Kučinar J, Sroka J, Deksne G, Keidāne D, Kváč M, Hůzová Z, Karanis P. Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016. Euro Surveill 2018 Jan;23(4).
                doi: 10.2807/1560-7917.ES.2018.23.4.16-00825pubmed: 29382412google scholar: lookup
              11. Deng L, Li W, Zhong Z, Gong C, Cao X, Song Y, Wang W, Huang X, Liu X, Hu Y, Fu H, He M, Wang Y, Zhang Y, Wu K, Peng G. Multi-locus genotypes of Enterocytozoon bieneusi in captive Asiatic black bears in southwestern China: High genetic diversity, broad host range, and zoonotic potential. PLoS One 2017;12(2):e0171772.
                doi: 10.1371/journal.pone.0171772pubmed: 28182656google scholar: lookup
              12. Deng L, Li W, Zhong Z, Gong C, Cao X, Song Y, Wang W, Huang X, Liu X, Hu Y, Fu H, He M, Wang Y, Zhang Y, Wu K, Peng G. Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China. J Eukaryot Microbiol 2017 Sep;64(5):716-720.
                doi: 10.1111/jeu.12399pubmed: 28166378google scholar: lookup
              13. Wasimuddin, Bryja J, Ribas A, Baird SJ, Piálek J, Goüy de Bellocq J. Testing parasite 'intimacy': the whipworm Trichuris muris in the European house mouse hybrid zone. Ecol Evol 2016 May;6(9):2688-701.
                doi: 10.1002/ece3.2022pubmed: 27064973google scholar: lookup
              14. Kváč M, Havrdová N, Hlásková L, Daňková T, Kanděra J, Ježková J, Vítovec J, Sak B, Ortega Y, Xiao L, Modrý D, Chelladurai JR, Prantlová V, McEvoy J. Cryptosporidium proliferans n. sp. (Apicomplexa: Cryptosporidiidae): Molecular and Biological Evidence of Cryptic Species within Gastric Cryptosporidium of Mammals. PLoS One 2016;11(1):e0147090.
                doi: 10.1371/journal.pone.0147090pubmed: 26771460google scholar: lookup
              15. Liu A, Zhang J, Zhao J, Zhao W, Wang R, Zhang L. The first report of Cryptosporidium andersoni in horses with diarrhea and multilocus subtype analysis. Parasit Vectors 2015 Sep 22;8:483.
                doi: 10.1186/s13071-015-1102-0pubmed: 26394848google scholar: lookup
              16. Zhang XX, Tan QD, Zhou DH, Ni XT, Liu GX, Yang YC, Zhu XQ. Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle, northwest China. Parasitol Res 2015 Jul;114(7):2781-7.
                doi: 10.1007/s00436-015-4537-5pubmed: 26002827google scholar: lookup
              17. Figueiredo AM, Köster PC, Dashti A, Torres RT, Fonseca C, Mysterud A, Bailo B, Carvalho J, Ferreira E, Hipólito D, Fernandes J, Lino A, Palmeira JD, Sarmento P, Neves N, Carrapato C, Calero-Bernal R, Carmena D. Molecular Detection and Distribution of Giardia duodenalis and Cryptosporidium spp. Infections in Wild and Domestic Animals in Portugal. Transbound Emerg Dis 2023;2023:5849842.
                doi: 10.1155/2023/5849842pubmed: 40303765google scholar: lookup
              18. Baz-González E, Foronda P. Genetic characterization of Cryptosporidium spp. in the North African hedgehog (Atelerix algirus) in the Canary Islands, Spain. Parasitol Res 2024 Jul 17;123(7):274.
                doi: 10.1007/s00436-024-08290-2pubmed: 39017738google scholar: lookup
              19. Stensvold CR, Larsen TG, Grüttner J, Nielsen L, Engberg J, Lebbad M. Rodent-adapted Cryptosporidium infection in humans: Seven new cases and review of the literature. One Health 2024 Jun;18:100682.
                doi: 10.1016/j.onehlt.2024.100682pubmed: 39010954google scholar: lookup
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