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Home / Equine Research Bank / Parasitol Res / Genetic diversity of Cryptosporidium spp. including novel id...
Parasitology research2015; 114(4); 1619-1624; doi: 10.1007/s00436-015-4353-y

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland.

Abstract: Faecal samples were collected from 352 horses on 23 farms operating under six different management systems in the Czech Republic and Poland during 2011 and 2012. Farms were selected without previous knowledge of parasitological status. All faecal samples were screened for Cryptosporidium spp. presence using microscopy, following aniline-carbol-methyl violet staining and PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes. Cryptosporidium muris-positive samples were additionally genotyped at four minisatellite markers: MS1 (encoding a hypothetical protein), MS2 (encoding a 90-kDa heat shock protein), MS3 (encoding a hypothetical protein) and MS16 (encoding a leucine-rich repeat family protein). Cryptosporidium spp. was detected by PCR in 12/352 (3.4%) samples from 4 out of 13 farms. None of the samples tested by microscopy was positive. There was no relationship between Cryptosporidium prevalence and age, sex, diarrhoea or management system; however, Cryptosporidium was found only on farms where horses were kept on pasture during the day and in a stable overnight. Sequence analyses of SSU and gp60 genes revealed the presence of C. muris RN66 (n = 9), Cryptosporidium parvum IIaA15G2R1 (n = 1), Cryptosporidium tyzzeri IXbA22R9 (n = 1), and Cryptosporidium horse genotype VIaA15G4 (n = 1). The C. muris subtypes were identified as MS1-M1, MS2-M4, novel MS2-M7 and MS16-M1 by multilocus sequence of three minisatellite loci. The MS3 locus was not amplified from any isolate. This is the first report of C. tyzzeri and C. muris subtypes from horses.
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Publication Date: 2015-02-27 PubMed ID: 25722018DOI: 10.1007/s00436-015-4353-yGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study delves into the genetic diversity of Cryptosporidium species present in horses in Czech Republic and Poland. It reports a novel identification of two species, Cryptosporidium muris and Cryptosporidium tyzzeri in horses, that were previously not reported.

Research Methodology

  • For this research, faecal samples were collected from a total of 352 horses. These horses belonged to 23 different farms operating under six distinct management systems in the Czech Republic and Poland, during the years 2011 and 2012.
  • The researchers performed their selection of farms without any prior knowledge of the parasitological status of these facilities.
  • All collected faecal samples were thoroughly screened for the presence of Cryptosporidium spp., utilizing microscopy after staining with aniline-carbol-methyl violet and performing PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes.

Research Findings

  • The analysis found that out of the 352 samples, Cryptosporidium spp. was detected in 12 samples (3.4%) from 4 out of the 13 farms.
  • Interestingly, none of the samples tested positive for Cryptosporidium when tested under microscopy.
  • The study also found that the occurrence of Cryptosporidium had no relationship with age, sex, presence of diarrhoea, or the management system of the farm. However, the parasitic species were only found on farms where horses were kept on pasture during the day and in a stable overnight.
  • Sequence analyses of SSU and gp60 genes starkly revealed the presence of C. muris RN66, Cryptosporidium parvum IIaA15G2R1, Cryptosporidium tyzzeri IXbA22R9, and Cryptosporidium horse genotype VIaA15G4 in the positive samples.
  • Additionally, the C. muris subtypes were further identified as MS1-M1, MS2-M4, a novel subtype MS2-M7, and MS16-M1 by a multilocus sequence of three minisatellite loci.
  • However, the MS3 locus could not be amplified from any isolate.

Significance of the Study

  • This study has important implications as it reports for the first time the presence of C. tyzzeri and C. muris subtypes in horses, indicating a wider species variation of Cryptosporidium spp. in horses than previously known.
  • These findings contribute to the body of knowledge about Cryptosporidium subspecies and their prevalence in equine hosts, which can have implications in understanding their transmission and effect on horse health.

Cite This Article

APA
Wagnerová P, Sak B, McEvoy J, Rost M, Matysiak AP, Ježková J, Kváč M. (2015). Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland. Parasitol Res, 114(4), 1619-1624. https://doi.org/10.1007/s00436-015-4353-y

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 114
Issue: 4
Pages: 1619-1624

Researcher Affiliations

Wagnerová, Pavla
  • Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 13, 370 05, České Budějovice, Czech Republic.
Sak, Bohumil
    McEvoy, John
      Rost, Michael
        Matysiak, Agniezska Perec
          Ježková, Jana
            Kváč, Martin

              MeSH Terms

              • Animals
              • Cryptosporidiosis / epidemiology
              • Cryptosporidium / classification
              • Cryptosporidium / genetics
              • Cryptosporidium / isolation & purification
              • Czech Republic / epidemiology
              • Diarrhea / parasitology
              • Diarrhea / veterinary
              • Feces / parasitology
              • Female
              • Genetic Variation
              • Genotype
              • Horse Diseases / parasitology
              • Horses
              • Male
              • Poland / epidemiology
              • Prevalence

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