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Home / Equine Research Bank / J Virol / Genome of horsepox virus.
Journal of virology2006; 80(18); 9244-9258; doi: 10.1128/JVI.00945-06

Genome of horsepox virus.

Abstract: Here we present the genomic sequence of horsepox virus (HSPV) isolate MNR-76, an orthopoxvirus (OPV) isolated in 1976 from diseased Mongolian horses. The 212-kbp genome contained 7.5-kbp inverted terminal repeats and lacked extensive terminal tandem repetition. HSPV contained 236 open reading frames (ORFs) with similarity to those in other OPVs, with those in the central 100-kbp region most conserved relative to other OPVs. Phylogenetic analysis of the conserved region indicated that HSPV is closely related to sequenced isolates of vaccinia virus (VACV) and rabbitpox virus, clearly grouping together these VACV-like viruses. Fifty-four HSPV ORFs likely represented fragments of 25 orthologous OPV genes, including in the central region the only known fragmented form of an OPV ribonucleotide reductase large subunit gene. In terminal genomic regions, HSPV lacked full-length homologues of genes variably fragmented in other VACV-like viruses but was unique in fragmentation of the homologue of VACV strain Copenhagen B6R, a gene intact in other known VACV-like viruses. Notably, HSPV contained in terminal genomic regions 17 kbp of OPV-like sequence absent in known VACV-like viruses, including fragments of genes intact in other OPVs and approximately 1.4 kb of sequence present only in cowpox virus (CPXV). HSPV also contained seven full-length genes fragmented or missing in other VACV-like viruses, including intact homologues of the CPXV strain GRI-90 D2L/I4R CrmB and D13L CD30-like tumor necrosis factor receptors, D3L/I3R and C1L ankyrin repeat proteins, B19R kelch-like protein, D7L BTB/POZ domain protein, and B22R variola virus B22R-like protein. These results indicated that HSPV contains unique genomic features likely contributing to a unique virulence/host range phenotype. They also indicated that while closely related to known VACV-like viruses, HSPV contains additional, potentially ancestral sequences absent in other VACV-like viruses.
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Publication Date: 2006-08-31 PubMed ID: 16940536PubMed Central: PMC1563943DOI: 10.1128/JVI.00945-06Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study completed a genomic sequence of the horsepox virus (HSPV), an orthopoxvirus known to infect Mongolian horses. It found HSPV to be closely related to the vaccinia virus and shared unique genomic features that could contribute to its unique virulence and host range.

Genomic Sequence of HSPV

  • The researchers presented the genomic sequence of the horsepox virus (HSPV) isolate MNR-76. This orthopoxvirus (OPV) was isolated back in 1976 from diseased Mongolian horses.
  • The genome measured 212 kilo base pairs (kbp) and had 7.5-kbp inverted terminal repeats. It lacked extensive terminal tandem repetition.
  • They found that HSPV had 236 open reading frames (ORFs) with similarities to other OPVs, with the center 100-kbp region being the most conserved compared to other OPVs.

Phylogenetic Analysis and Relationship with Other Viruses

  • Phylogenetic analysis of the conserved region showed that HSPV is closely related to sequenced isolates of vaccinia virus (VACV) and rabbitpox virus.
  • It is unique from these due to fragmentation of the VACV strain Copenhagen B6R, a gene that is intact in other known VACV-like viruses.
  • The researchers also noted that HSPV contained 17 kbp of OPV-like sequence in terminal genomic regions that were absent in other VACV-like viruses.

Unique Genomic Features and Genes

  • HSPV was found to have unique genomic features that presumably contribute to its distinct virulence/host range phenotype.
  • It contained nine full-length genes fragmented or missing in other VACV-like viruses. Some of these genes were only present in cowpox virus.
  • Despite its close relation to known VACV-like viruses, HSPV’s genome had additional sequences that were absent from other VACV-like viruses.

The presented information provides valuable insights into the genetics of the horsepox virus and its relationship to other viruses. These findings can further be utilized to understand the genetic basis of virulence and host range in this class of viruses.

Cite This Article

APA
Tulman ER, Delhon G, Afonso CL, Lu Z, Zsak L, Sandybaev NT, Kerembekova UZ, Zaitsev VL, Kutish GF, Rock DL. (2006). Genome of horsepox virus. J Virol, 80(18), 9244-9258. https://doi.org/10.1128/JVI.00945-06

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 80
Issue: 18
Pages: 9244-9258

Researcher Affiliations

Tulman, E R
  • Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY 11944, USA.
Delhon, G
    Afonso, C L
      Lu, Z
        Zsak, L
          Sandybaev, N T
            Kerembekova, U Z
              Zaitsev, V L
                Kutish, G F
                  Rock, D L

                    MeSH Terms

                    • Animals
                    • Cloning, Molecular
                    • Cowpox virus / genetics
                    • DNA, Viral
                    • Genome, Viral
                    • Horses
                    • Models, Genetic
                    • Molecular Sequence Data
                    • Open Reading Frames
                    • Phenotype
                    • Phylogeny
                    • Vaccinia virus / genetics
                    • Variola virus / genetics
                    • Viruses / genetics

                    References

                    This article includes 94 references
                    1. Afonso CL, Tulman ER, Lu Z, Oma E, Kutish GF, Rock DL. The genome of Melanoplus sanguinipes entomopoxvirus.. J. Virol. 73:533-552.
                      pmc: PMC103860pubmed: 9847359
                    2. Afonso CL, Tulman ER, Lu Z, Zsak L, Sandybaev NT, Kerembekova UZ, Zaitsev VL, Kutish GF, Rock DL. The genome of camelpox virus.. Virology 295:1-9.
                      pubmed: 12033760
                    3. Albagli O, Dhordain P, Deweindt C, Lecocq G, Leprince D. The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins.. Cell Growth Differ. 6:1193-1198.
                      pubmed: 8519696
                    4. Alcami A. Viral mimicry of cytokines, chemokines and their receptors.. Nat. Rev. Immunol. 3:36-50.
                      pubmed: 12511874
                    5. Alcami A, Khanna A, Paul NL, Smith GL. Vaccinia virus strains Lister, USSR and Evans express soluble and cell-surface tumour necrosis factor receptors.. J. Gen. Virol. 80:949-959.
                      pubmed: 10211965
                    6. Alcami A, Symons JA, Collins PD, Williams TJ, Smith GL. Blockade of chemokine activity by a soluble chemokine binding protein from vaccinia virus.. J. Immunol. 160:624-633.
                      pubmed: 9551896
                    7. Alejo A, Ruiz-Arguello MB, Ho Y, Smith VP, Saraiva M, Alcami A. A chemokine-binding domain in the tumor necrosis factor receptor from variola (smallpox) virus.. Proc. Natl. Acad. Sci. USA 103:5995-6000.
                      pmc: PMC1458686pubmed: 16581912
                    8. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.. Nucleic Acids Res. 25:3389-3402.
                      pmc: PMC146917pubmed: 9254694
                    9. Antoine G, Scheiflinger F, Dorner F, Falkner FG. The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses.. Virology 244:365-396.
                      pubmed: 9601507
                    10. Baroudy BM, Moss B. Sequence homologies of diverse length tandem repetitions near ends of vaccinia virus genome suggest unequal crossing over.. Nucleic Acids Res. 10:5673-5679.
                      pmc: PMC320915pubmed: 6292846
                    11. Baroudy BM, Venkatensan S, Moss B. Incompletely base-paired flip-flop terminal loops link the two DNA strands of the vaccinia virus genome into one uninterrupted polynucleotide chain.. Cell 28:315-324.
                      pubmed: 7060133
                    12. Barry M, Hnatiuk S, Mossman K, Lee SF, Boshkov L, McFadden G. The myxoma virus M-T4 gene encodes a novel RDEL-containing protein that is retained within the endoplasmic reticulum and is important for the productive infection of lymphocytes.. Virology 239:360-377.
                      pubmed: 9434727
                    13. Baxby D. Is cowpox misnamed? A review of 10 human cases.. Br. Med. J. 1:1379-1381.
                      pmc: PMC1606875pubmed: 861644
                    14. Baxby D. Jenner's smallpox vaccine: the riddle of vaccinia virus and its origin.. .
                    15. Brady G, Boggan L, Bowie A, O'Neill LA. Schlafen-1 causes a cell cycle arrest by inhibiting induction of cyclin D1.. J. Biol. Chem. 280:30723-30734.
                      pubmed: 15946944
                    16. Breman JG, Henderson DA. Diagnosis and management of smallpox.. N. Engl. J. Med. 346:1300-1308.
                      pubmed: 11923491
                    17. Brudno M, Chapman M, Gottgens B, Batzoglou S, Morgenstern B. Fast and sensitive multiple alignment of large genomic sequences.. BMC Bioinformatics 4:66.
                      pmc: PMC521198pubmed: 14693042
                    18. Brudno M, Do CB, Cooper GM, Kim MF, Davydov E, Green ED, Sidow A, Batzoglou S. LAGAN and Multi-LAGAN: efficient tools for large-scale multiple alignment of genomic DNA.. Genome Res. 13:721-731.
                      pmc: PMC430158pubmed: 12654723
                    19. Buller RM, Palumbo GJ. Poxvirus pathogenesis.. Microbiol. Rev. 55:80-122.
                      pmc: PMC372802pubmed: 1851533
                    20. Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis.. Mol. Biol. Evol. 17:540-552.
                      pubmed: 10742046
                    21. Chen N, Danila MI, Feng Z, Buller RM, Wang C, Han X, Lefkowitz EJ, Upton C. The genomic sequence of ectromelia virus, the causative agent of mousepox.. Virology 317:165-186.
                      pubmed: 14675635
                    22. Chen N, Li G, Liszewski MK, Atkinson JP, Jahrling PB, Feng Z, Schriewer J, Buck C, Wang C, Lefkowitz EJ, Esposito JJ, Harms T, Damon IK, Roper RL, Upton C, Buller RM. Virulence differences between monkeypox virus isolates from West Africa and the Congo basin.. Virology 340:46-63.
                      pmc: PMC9534023pubmed: 16023693
                    23. Child SJ, Palumbo GJ, Buller RM, Hruby DE. Insertional inactivation of the large subunit of ribonucleotide reductase encoded by vaccinia virus is associated with reduced virulence in vivo.. Virology 174:625-629.
                      pubmed: 2154895
                    24. da Fonseca FG, Trindade GS, Silva RL, Bonjardim CA, Ferreira PC, Kroon EG. Characterization of a vaccinia-like virus isolated in a Brazilian forest.. J. Gen. Virol. 83:223-228.
                      pubmed: 11752719
                    25. Damaso CR, Esposito JJ, Condit RC, Moussatche N. An emergent poxvirus from humans and cattle in Rio de Janeiro State: Cantagalo virus may derive from Brazilian smallpox vaccine.. Virology 277:439-449.
                      pubmed: 11080491
                    26. de Carlos A, Paez E. Isolation and characterization of mutants of vaccinia virus with a modified 94-kDa inclusion protein.. Virology 185:768-778.
                      pubmed: 1962448
                    27. de Souza Trindade G, da Fonseca FG, Marques JT, Nogueira ML, Mendes LC, Borges AS, Peiro JR, Pituco EM, Bonjardim CA, Ferreira PC, Kroon EG. Aracatuba virus: a vaccinialike virus associated with infection in humans and cattle.. Emerg. Infect. Dis. 9:155-160.
                      pmc: PMC2901946pubmed: 12603984
                    28. Essajee S, Kaufman HL. Poxvirus vaccines for cancer and HIV therapy.. Expert Opin. Biol. Ther. 4:575-588.
                      pubmed: 15102606
                    29. Ewing B, Green P. Base-calling of automated sequencer traces using Phred. II. Error probabilities.. Genome Res. 8:186-194.
                      pubmed: 9521922
                    30. Ewing B, Hillier L, Wendl MC, Green P. Base-calling of automated sequencer traces using Phred. I. Accuracy assessment.. Genome Res. 8:175-185.
                      pubmed: 9521921
                    31. Felsenstein J. PHYLIP—phylogeny inference package (version 3.2).. Cladistics 5:164-166.
                    32. Fenner F, Henderson DA, Arita I, Jezek Z, Ladnyi ID. Smallpox and its eradication.. World Health Organization, Geneva, Switzerland.
                    33. Fenner F, Wittek R, Dumbell K. The orthopoxviruses.. Academic Press, Inc., San Diego, Calif.
                    34. Galtier N, Gouy M, Gautier C. SEAVIEW and PHYLO WIN: two graphic tools for sequence alignment and molecular phylogeny.. Comput. Appl. Biol. Sci. 12:543-554.
                      pubmed: 9021275
                    35. Gardner JD, Tscharke DC, Reading PC, Smith GL. Vaccinia virus semaphorin A39R is a 50-55 kDa secreted glycoprotein that affects the outcome of infection in a murine intradermal model.. J. Gen. Virol. 82:2083-2093.
                      pubmed: 11514717
                    36. Goebel SJ, Johnson GP, Perkus ME, Davis SW, Winslow JP, Paoletti E. The complete DNA sequence of vaccinia virus.. Virology 179:247-266.
                      pubmed: 2219722
                    37. Gordon D, Abajian C, Green P. Consed: a graphical tool for sequence finishing.. Genome Res. 8:192-202.
                      pubmed: 9521923
                    38. Gubser C, Hue S, Kellam P, Smith GL. Poxvirus genomes: a phylogenetic analysis.. J. Gen. Virol. 85:105-117.
                      pubmed: 14718625
                    39. Gubser C, Smith GL. The sequence of camelpox virus shows it is most closely related to variola virus, the cause of smallpox.. J. Gen. Virol. 83:855-872.
                      pubmed: 11907336
                    40. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood.. Syst. Biol. 52:696-704.
                      pubmed: 14530136
                    41. Hsiao JC, Chung CS, Drillien R, Chang W. The cowpox virus host range gene, CP77, affects phosphorylation of eIF2 alpha and vaccinia viral translation in apoptotic HeLa cells.. Virology 329:199-212.
                      pubmed: 15476887
                    42. Hu FQ, Smith CA, Pickup DJ. Cowpox virus contains two copies of an early gene encoding a soluble secreted form of the type II TNF receptor.. Virology 204:343-356.
                      pubmed: 8091665
                    43. Huang X, Madan A. CAP3: a DNA sequence assembly program.. Genome Res. 9:868-877.
                      pmc: PMC310812pubmed: 10508846
                    44. Hutyra F, Marek J, Manninger R. Pox (variola), p. 348-393. In J. R. Greig (ed.), Special pathology and therapeutics of the diseases of domestic animals, vol. 1.. Bailliere, Tindall and Cox, London, United Kingdom.
                    45. Kochneva G, Kolosova I, Maksyutova T, Ryabchikova E, Shchelkunov S. Effects of deletions of kelch-like genes on cowpox virus biological properties.. Arch. Virol. 150:1857-1870.
                      pubmed: 15824883
                    46. Kolhapure RM, Deolankar RP, Tupe CD, Raut CG, Basu A, Dama BM, Pawar SD, Joshi MV, Padbidri VS, Goverdhan MK, Banerjee K. Investigation of buffalopox outbreaks in Maharashtra State during 1992-1996.. Indian J. Med. Res. 106:441-446.
                      pubmed: 9415737
                    47. Kotwal GJ, Moss B. Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant.. Virology 167:524-537.
                      pubmed: 2849238
                    48. Legrand FA, Verardi PH, Jones LA, Chan KS, Peng Y, Yilma TD. Induction of potent humoral and cell-mediated immune responses by attenuated vaccinia virus vectors with deleted serpin genes.. J. Virol. 78:2770-2779.
                      pmc: PMC353749pubmed: 14990697
                    49. Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, Dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG. Passatempo virus, a vaccinia virus strain, Brazil.. Emerg. Infect. Dis. 11:1935-1938.
                      pmc: PMC3367646pubmed: 16485483
                    50. Lewis-Jones S. Zoonotic poxvirus infections in humans.. Curr. Opin. Infect. Dis. 17:81-89.
                      pubmed: 15021045
                    51. Li G, Chen N, Roper RL, Feng Z, Hunter A, Danila M, Lefkowitz EJ, Buller RM, Upton C. Complete coding sequences of the rabbitpox virus genome.. J. Gen. Virol. 86:2969-2977.
                      pubmed: 16227218
                    52. Likos AM, Sammons SA, Olson VA, Frace AM, Li Y, Olsen-Rasmussen M, Davidson W, Galloway R, Khristova ML, Reynolds MG, Zhao H, Carroll DS, Curns A, Formenty P, Esposito JJ, Regnery RL, Damon IK. A tale of two clades: monkeypox viruses.. J. Gen. Virol. 86:2661-2672.
                      pubmed: 16186219
                    53. Massung RF, Knight JC, Esposito JJ. Topography of variola smallpox virus inverted terminal repeats.. Virology 211:350-355.
                      pubmed: 7645234
                    54. Massung RF, Liu L-I, Qi J, Knight JC, Yuran TE, Kerlavage AR, Parsons JM, Venter JC, Esposito JJ. Analysis of the complete genome of smallpox variola major virus strain Bangladesh-1975.. Virology 201:215-240.
                      pubmed: 8184534
                    55. Merchlinsky M, Moss B. Nucleotide sequence required for resolution of the concatemer junction of vaccinia virus DNA.. J. Virol. 63:4354-4361.
                      pmc: PMC251052pubmed: 2778879
                    56. Meyer H, Totmenin A, Gavrilova E, Shchelkunov S. Variola and camelpox virus-specific sequences are part of a single large open reading frame identified in two German cowpox virus strains.. Virus Res. 108:39-43.
                      pubmed: 15681053
                    57. Morgenstern B, Frech K, Dress A, Werner T. DIALIGN: finding local similarities by multiple sequence alignment.. Bioinformatics 14:290-294.
                      pubmed: 9614273
                    58. Morikawa S, Sakiyama T, Hasegawa H, Saijo M, Maeda A, Kurane I, Maeno G, Kimura J, Hirama C, Yoshida T, Asahi-Ozaki Y, Sata T, Kurata T, Kojima A. An attenuated LC16m8 smallpox vaccine: analysis of full-genome sequence and induction of immune protection.. J. Virol. 79:11873-11891.
                      pmc: PMC1212643pubmed: 16140764
                    59. Moss B. Poxviridae: the viruses and their replication, p. 2849-2883. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 4th ed., vol. 2.. Lippincott Williams & Wilkins, Philadelphia, Pa..
                    60. Moss B, Shisler JL. Immunology 101 at poxvirus U: immune evasion genes.. Semin. Immunol. 13:59-66.
                      pubmed: 11289800
                    61. Moyer RW, Graves RL. The mechanism of cytoplasmic orthopoxvirus DNA replication.. Cell 27:391-401.
                      pubmed: 6277506
                    62. Nylander JAA. MrModeltest 2.2.. Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden.
                    63. Panus JF, Smith CA, Ray CA, Smith TD, Patel DD, Pickup DJ. Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue.. Proc. Natl. Acad. Sci. USA 99:8348-8353.
                      pmc: PMC123070pubmed: 12034885
                    64. Pearson WR. Rapid and sensitive sequence comparison with FASTP and FASTA.. Methods Enzymol. 183:63-98.
                      pubmed: 2156132
                    65. Pearson WR, Lipman DJ. Improved tools for biological sequence comparison.. Proc. Natl. Acad. Sci. USA 85:2444-2448.
                      pmc: PMC280013pubmed: 3162770
                    66. Pearson WR, Wood T, Zhang Z, Miller W. Comparison of DNA sequences with protein sequences.. Genomics 46:24-36.
                      pubmed: 9403055
                    67. Perkus ME, Goebel SJ, Davis SW, Johnson GP, Limbach K, Norton EK, Paoletti E. Vaccinia virus host range genes.. Virology 179:276-286.
                      pubmed: 2171207
                    68. Price N, Tscharke DC, Smith GL. The vaccinia virus B9R protein is a 6 kDa intracellular protein that is non-essential for virus replication and virulence.. J. Gen. Virol. 83:873-878.
                      pubmed: 11907337
                    69. Reed KD, Melski JW, Graham MB, Regnery RL, Sotir MJ, Wegner MV, Kazmierczak JJ, Stratman EJ, Li Y, Fairley JA, Swain GR, Olson VA, Sargent EK, Kehl SC, Frace MA, Kline R, Foldy SL, Davis JP, Damon IK. The detection of monkeypox in humans in the Western Hemisphere.. N. Engl. J. Med. 350:342-350.
                      pubmed: 14736926
                    70. Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite.. Trends Genet. 16:276-277.
                      pubmed: 10827456
                    71. Salzberg SL, Delcher AL, Kasif S, White O. Microbial gene identification using interpolated Markov models.. Nucleic Acids Res. 26:544-548.
                      pmc: PMC147303pubmed: 9421513
                    72. Saraiva M, Smith P, Fallon PG, Alcami A. Inhibition of type 1 cytokine-mediated inflammation by a soluble CD30 homologue encoded by ectromelia (mousepox) virus.. J. Exp. Med. 196:829-839.
                      pmc: PMC2194064pubmed: 12235215
                    73. Schmidt HA, Strimmer K, Vingron M, von Haeseler A. TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing.. Bioinformatics 18:502-504.
                      pubmed: 11934758
                    74. Senkevich TG, Wolffe EJ, Buller RML. Ectromelia virus RING finger protein is localized in virus factories and is required for virus replication in macrophages.. J. Virol. 69:4103-4111.
                      pmc: PMC189145pubmed: 7769668
                    75. Shchelkunov S, Totmenin A, Kolosova I. Species-specific differences in organization of orthopoxvirus kelch-like proteins.. Virus Genes 24:157-162.
                      pubmed: 12018707
                    76. Shchelkunov SN, Blinov VM, Resenchuk SM, Totmenin AV, Olenina LV, Chirikova GB, Sandakhchiev LS. Analysis of the nucleotide sequence of 53 kbp from the right terminus of the genome of variola major virus strain India-1967.. Virus Res. 34:207-236.
                      pubmed: 7856312
                    77. Shchelkunov SN, Blinov VM, Sandakhchiev LS. Ankyrin-like proteins of variola and vaccinia viruses.. FEBS Lett. 319:163-165.
                      pubmed: 8384122
                    78. Shchelkunov SN, Massung RF, Esposito JJ. Comparison of the genome DNA sequences of Bangladesh-1975 and India-1967 variola viruses.. Virus Res. 36:107-118.
                      pubmed: 7625123
                    79. Shchelkunov SN, Safronov PF, Totmenin AV, Petrov NA, Ryazankina OI, Gutorov VV, Kotwal GJ. The genome sequence analysis of the left and right species-specific terminal region of a cowpox virus strain reveals unique sequences and a cluster of intact ORFs for immunomodulatory and host range proteins.. Virology 243:432-460.
                      pubmed: 9568042
                    80. Shchelkunov SN, Totmenin AV, Safronov PF, Gutorov VV, Chizhikov VE, Knight JC, Parsons JM, Massung RF, Esposito JJ. Alastrim smallpox variola minor virus genome DNA sequences.. Virology 266:361-386.
                      pubmed: 10639322
                    81. Shchelkunov SN, Totmenin AV, Safronov PF, Mikheev MV, Gutorov VV, Ryazankina OI, Petrov NA, Babkin IV, Uvarova EA, Sandakhchiev LS, Sisler JR, Esposito JJ, Damon IK, Jahrling PB, Moss B. Analysis of the monkeypox virus genome.. Virology 297:172-194.
                      pmc: PMC9534300pubmed: 12083817
                    82. Shisler JL, Moss B. Immunology 102 at poxvirus U: avoiding apoptosis.. Semin. Immunol. 13:67-72.
                      pubmed: 11289801
                    83. Smith GL, McFadden G. Smallpox: anything to declare?. Nat. Rev. Immunol. 2:521-527.
                      pubmed: 12094226
                    84. Sonnhammer EL, Durbin R. A dot-matrix program with dynamic threshold control suited for genomic DNA and protein sequence analysis.. Gene 167:GC1-GC10.
                      pubmed: 8566757
                    85. Spehner D, Gillard S, Drillien R, Kirn A. A cowpox virus gene required for multiplication in Chinese hamster ovary cells.. J. Virol. 62:1297-1304.
                      pmc: PMC253141pubmed: 2831390
                    86. Studdert MJ. Experimental vaccinia virus infection of horses.. Aust. Vet. J. 66:157-159.
                      pubmed: 2735900
                    87. Taylor JM, Barry M. Near death experiences: poxvirus regulation of apoptotic death.. Virology 344:139-150.
                      pubmed: 16364745
                    88. Thompson CH, Yager JA, Van Rensburg IB. Close relationship between equine and human molluscum contagiosum virus demonstrated by in situ hybridisation.. Res. Vet. Sci. 64:157-161.
                      pubmed: 9625473
                    89. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res. 22:4673-4680.
                      pmc: PMC308517pubmed: 7984417
                    90. Trindade GS, da Fonseca FG, Marques JT, Diniz S, Leite JA, De Bodt S, Van der Peer Y, Bonjardim CA, Ferreira PC, Kroon EG. Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice.. J. Gen. Virol. 85:2015-2021.
                      pubmed: 15218187
                    91. Upton C, Schiff L, Rice SA, Dowdeswell T, Yang X, McFadden G. A poxvirus protein with a RING finger motif binds zinc and localizes in virus factories.. J. Virol. 68:4186-4195.
                      pmc: PMC236341pubmed: 8207794
                    92. Upton C, Slack S, Hunter AL, Ehlers A, Roper RL. Poxvirus orthologous clusters: toward defining the minimum essential poxvirus genome.. J. Virol. 77:7590-7600.
                      pmc: PMC164831pubmed: 12805459
                    93. Wesley RD, Tuthill AE. Genome relatedness among African swine fever virus field isolates by restriction endonuclease analysis.. Prev. Vet. Med. 2:53-62.
                    94. Yager JA, Scott DW, Wilcock BP. The skin and appendages, p. 531-738. In K. V. F. Jubb, P. C. Kennedy, and N. Palmer (ed.), Pathology of domestic animals, 4th ed., vol. 1.. Academic Press, San Diego, Calif.
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