Genome-wide association studies for osteochondrosis in French Trotter horses.
Abstract: A genome-wide association study for osteochondrosis (OC) in French Trotter horses was carried out to detect QTL using genotype data from the Illumina EquineSNP50 BeadChip assay. Analysis data came from 161 sire families of French Trotter horses with 525 progeny and family sizes ranging from 1 to 20. Genotypes were available for progeny (n = 525) and sires with at least 2 progeny (n = 98). Radiographic data were obtained from progeny using at least 10 views to reveal OC. All radiographic findings were described by at least 2 veterinary experts in equine orthopedics, and severity indices (scores) were assigned based on the size and location of the lesion. Traits used were a global score, the sum of all severity scores lesions (GM, quantitative measurement), and the presence or absence of OC on the fetlock (FM), hock (HM), and other sites (other). Data were analyzed using 2 mixed models including fixed effects, polygenic effects, and SNP or haplotype cluster effects. By combining results with both methods at moderate evidence of association threshold P < 5 × 10(-5), this genome-wide association study displayed 1 region for GM on the Equus caballus chromosome (ECA) 13, 2 for HM on ECA 3 and 14, and 1 for other on ECA 15. One region on ECA 3 for HM represented the most significant hit (P = 3 × 10(-6)). By comparing QTL between traits at a decreased threshold (P < 5 × 10(-4)), the 4 QTL detected for GM were associated to a QTL detected for FM or HM but never both. Another interesting result was that no QTL were found in common between HM and FM.
Publication Date: 2011-08-12 PubMed ID: 21841084DOI: 10.2527/jas.2011-4031Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study explores the genetic aspects of osteochondrosis (a joint disorder) in French Trotter horses, aiming to detect quantitative trait loci (QTL) using genetic data from a recently developed high-throughput genotyping platform. The results identified significant genomic regions associated with osteochondrosis on various horse chromosomes.
Study Methodology
- The researchers conducted a genome-wide association study (GWAS) to examine the entire genome of French Trotter horses for small variations, called single nucleotide polymorphisms (SNPs), that occur more frequently in horses with osteochondrosis.
- Data was collected from 161 sire families with 525 offspring. For all horses involved in the study, genotypes were collected and analysed.
- Radiographic examinations were conducted on each offspring to detect the presence of osteochondrosis. These findings were evaluated by two expert equine veterinarians who assigned severity scores for each lesion based on size and location.
- Two different statistical models were used to analyse the data, each considering the potential influences of fixed effects, polygenic effects, and SNP or haplotype cluster effects.
Primary Findings
- The study found one genomic region on horse chromosome 13 (ECA 13) associated with the overall disease score.
- Two regions, on chromosomes ECA 3 and ECA 14, were found to be associated with osteochondrosis in the hock joint (HM).
- One region on ECA 15 was identified as associated with osteochondrosis in other unspecified sites (other).
- The most significant finding was the region on ECA 3 associated with HM, with a p-value of 3×10^-6, suggesting a very low probability that the observed association occurred by chance.
Comparative Analysis
- When comparing QTLs between different traits, the four QTLs detected for global measurement of the condition (GM) were linked with a QTL detected for either fetlock osteochondrosis (FM) or hock osteochondrosis (HM), but not both.
- Interestingly, no QTLs were common between HM and FM, indicating different genetic influences for osteochondrosis in these two anatomical locations.
Significance of the Study
- The study provides valuable insights into the genetic basis of osteochondrosis in horses, particularly French Trotter horses. The identified genomic regions could serve as starting points for further investigations into the genetic mechanisms behind this condition.
- Understanding the genetic predispositions to osteochondrosis could potentially aid in the development of disease prevention strategies, enhance horse breeding programs, and contribute to the overall well-being of horses.
Cite This Article
APA
Teyssèdre S, Dupuis MC, Guérin G, Schibler L, Denoix JM, Elsen JM, Ricard A.
(2011).
Genome-wide association studies for osteochondrosis in French Trotter horses.
J Anim Sci, 90(1), 45-53.
https://doi.org/10.2527/jas.2011-4031 Publication
Researcher Affiliations
- Institut National de la Recherche Agronomique (INRA), UR 631, 31326 Castanet-Tolosan, France. simon.teyssedre@toulouse.inra.fr
MeSH Terms
- Animals
- Female
- Forelimb / diagnostic imaging
- Forelimb / pathology
- France
- Genome-Wide Association Study
- Genotype
- Haplotypes
- Horse Diseases / diagnostic imaging
- Horse Diseases / genetics
- Horse Diseases / pathology
- Horses
- Joint Diseases / diagnostic imaging
- Joint Diseases / genetics
- Joint Diseases / pathology
- Joint Diseases / veterinary
- Male
- Models, Biological
- Osteochondrosis / diagnostic imaging
- Osteochondrosis / genetics
- Osteochondrosis / pathology
- Osteochondrosis / veterinary
- Polymorphism, Single Nucleotide
- Quantitative Trait Loci
- Radiography
- Tarsus, Animal / diagnostic imaging
- Tarsus, Animal / pathology
Citations
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