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Home/Equine Research Bank/Virus Genes/Genomic analysis of an effective lentiviral vaccine-attenuat...
Virus genes2010; 41(1); 86-98; doi: 10.1007/s11262-010-0491-6

Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV FDDV13.

Abstract: Chinese equine infectious anemia virus (EIAV) attenuated vaccine is the first lentiviral vaccine with a successful application. In order to understand the correlation of viral genomic mutations with viral attenuation and with induced immunoprotective properties, we analyzed the proviral genome sequences of the EIAV-attenuated vaccine strain EIAV(FDDV13) (EIAV fetal donkey dermal cell-adapted vaccine) and its highly virulent parental strain EIAV(LN40). The sequences of these strains were compared with those of the major foreign EIAV strains. The results indicated a large genetic distance between the Chinese EIAV strain and the major EIAV strains in America and Japan. The Chinese strains belong to an independent phylogenetic branch. The divergence between the entire genome of the Chinese strains and that of other major EIAV strains is approximately 23%. The divergence rate in LTR is over 14%, whereas that in each open reading frame is over 20%. The gp90 exhibited a divergence of 35% in its nucleotide sequence and 40% in its amino acid sequence. The present study found that after long-term passage in vitro, EIAV(FDDV13) has accumulated many stable substitution mutations in each gene. These mutations at multiple sites in multiple genes of the vaccine strain, especially the conserved mutations, provide important references for further understanding the attenuation mechanism of Chinese EIAV-attenuated vaccine and the immunoprotection mechanism of lentiviral vaccines.
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Publication Date: 2010-06-05 PubMed ID: 20526660DOI: 10.1007/s11262-010-0491-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The abstract describes the genomic analysis of a successful Chinese lentiviral vaccine strain, EIAV FDDV13, against the equine infectious anemia virus (EIAV). The study compares this attenuated vaccine strain with its virulent parent strain and other major EIAV strains from America and Japan, highlighting substantial genetic difference, and mutations that may contribute to the vaccine’s effectiveness.

Genome Analysis of the Chinese Lentiviral Vaccine EIAV FDDV13

  • The study conducts a genomic analysis of a Chinese EIAV vaccine, EIAV FDDV13, and its highly virulent parental strain, EIAV(LN40). This analysis focuses on understanding the correlation between genomic mutations in the virus and its attenuation and immunoprotective properties.
  • The sequences of these strains were compared with that of major EIAV strains in the US and Japan.
  • Researchers found significant genetic variance between the Chinese EIAV strain and the major EIAV strains in other countries, with the Chinese strains forming an independent phylogenetic branch.

Genetic Divergence

  • The divergence between the genome of the Chinese strains and that of the other major EIAV strains is placed at approximately 23%.
  • The study also found that the divergence rate in the Long terminal Repeat (LTR) is over 14%, while each open reading frame has a divergence rate of over 20%.
  • The gp90 showed substantial change, displaying a divergence of 35% in nucleotide sequence and 40% in its amino acid sequence.

Mutations and their Effects

  • The research identified that after long-term passage in vitro, EIAV(FDDV13) accumulated many stable substitution mutations in each gene.
  • The multiple mutations at different sites are thought to contribute to the successful attenuation of the vaccine strain, especially the conserved mutations.
  • The findings from this research provide important reference points for understanding the attenuation of Chinese EIAV vaccine and the immune protection mechanism of lentiviral vaccines in general.

Cite This Article

APA
Qi X, Wang X, Wang S, Lin Y, Jiang C, Ma J, Zhao L, Lv X, Shen R, Wang F, Kong X, Su Z, Zhou J. (2010). Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV FDDV13. Virus Genes, 41(1), 86-98. https://doi.org/10.1007/s11262-010-0491-6

Publication

Virus genes
ISSN: 1572-994X
NlmUniqueID: 8803967
Country: United States
Language: English
Volume: 41
Issue: 1
Pages: 86-98

Researcher Affiliations

Qi, Xu
  • Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Wang, Xuefeng
    Wang, Shuai
      Lin, Yuezhi
        Jiang, Chenggang
          Ma, Jian
            Zhao, Liping
              Lv, Xiaoling
                Shen, Rongxian
                  Wang, Fenglong
                    Kong, Xiangang
                      Su, Zhiqiang
                        Zhou, Jianhua

                          MeSH Terms

                          • Base Sequence
                          • Genome, Viral
                          • Infectious Anemia Virus, Equine / genetics
                          • Infectious Anemia Virus, Equine / immunology
                          • Molecular Sequence Data
                          • Mutation
                          • Phylogeny
                          • Sequence Alignment
                          • Vaccines, Attenuated / immunology
                          • Viral Vaccines / immunology

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                          Citations

                          This article has been cited 9 times.
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