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Viral DNA in horses infected with equine infectious anemia virus.
Abstract: The amount and distribution of viral DNA were established in a horse acutely infected with the Wyoming strain of equine infectious anemia virus (EIAV). The highest concentration of viral DNA were found in the liver, lymph nodes, bone marrow, and spleen. The kidney, choroid plexus, and peripheral blood leukocytes also contained viral DNA, but at a lower level. It is estimated that at day 16 postinoculation, almost all of the viral DNA was located in the tissues, with the liver alone containing about 90 times more EIAV DNA than the peripheral blood leukocytes did. Assuming a monocyte-macrophage target, each infected cell contained multiple copies of viral DNA (between 6 and 60 copies in liver Kupffer cells). At day 16 postinoculation, most of the EIAV DNA was not integrated into host DNA, but existed in both linear and circular unintegrated forms. In contrast to acute infection, viral DNA was not detectable in tissues from asymptomatic horses with circulating antibody to EIAV.
Publication Date: 1989-12-01 PubMed ID: 2555550PubMed Central: PMC251183DOI: 10.1128/JVI.63.12.5194-5200.1989Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigated the presence and distribution of viral DNA in horses infected with a specific strain of Equine Infectious Anemia Virus (EIAV). The highest concentration of viral DNA was found in certain tissues, primarily the liver.
Research Objectives
The goal of this research was to explore and understand the concentration and distribution of viral DNA in a horse acutely infected with the Wyoming strain of Equine Infectious Anemia Virus (EIAV).
Methodology
- Horses were infected with a specific strain of EIAV, and over the course of 16 days post-infection, the concentration of viral DNA in various tissues was recorded.
- The tissues studied included the liver, lymph nodes, bone marrow, spleen, kidney, choroid plexus, and peripheral blood leukocytes.
Findings
- The highest concentration of DNA from the EIAV was found in the liver, lymph nodes, bone marrow, and spleen.
- Lower levels of EIAV DNA were found in the kidney, choroid plexus, and peripheral blood leukocytes.
- On the 16th day following inoculation with the virus, almost all viral DNA was situated within tissues, with the liver alone holding around 90 times more EIAV DNA than the peripheral blood leukocytes.
- Assuming that the virus targets monocyte-macrophage cells, the research found that each infected cell housed multiple copies of the viral DNA – between 6 and 60 copies in liver Kupffer cells.
- The EIAV DNA on the 16th day following viral inoculation was found to exist in linear and circular unintegrated forms and was mostly not integrated into the host’s DNA.
- However, viral DNA was not detectable in tissues from asymptomatic horses with circulating antibodies against EIAV.
Implications
The findings of this research provide insight into the behavior of EIAV in infected horses, specifically how viral DNA is distributed within the animal’s body and the elevated concentration in certain tissues. This can aid in understanding the course of the infection and potentially inform strategies for treatment or prevention of EIAV.
Cite This Article
APA
Rice NR, Lequarre AS, Casey JW, Lahn S, Stephens RM, Edwards J.
(1989).
Viral DNA in horses infected with equine infectious anemia virus.
J Virol, 63(12), 5194-5200.
https://doi.org/10.1128/JVI.63.12.5194-5200.1989 Publication
Researcher Affiliations
- BRI-Basic Research Program, National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.
MeSH Terms
- Animals
- DNA, Viral / isolation & purification
- Equine Infectious Anemia / microbiology
- Genes, Viral
- Horses
- Immunoblotting
- Infectious Anemia Virus, Equine / isolation & purification
- Leukocytes / microbiology
- Organ Specificity
Grant Funding
- N01-CO-74101 / NCI NIH HHS
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Citations
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