Genomic variation and segregation of equine infectious anemia virus during acute infection.

Kim, CH
·
Casey, JW

Abstract: Equine infectious anemia virus (EIAV) is a lentivirus that infects and persists in the monocyte/macrophage populations of blood and tissues. We employed polymerase chain reaction to investigate the distribution and the level of genome variability of EIAV DNA in different tissues of a horse infected with a highly virulent variant of the Wyoming strain of the virus. Long terminal repeat, gag, and pol primer pairs were used to direct the amplification of EIAV DNA from the peripheral blood mononuclear cells and from cells, presumably the macrophage subtypes, of the kidney, spleen, liver, lymph node, and cerebellum and periventricular regions of the brain. Polymerase chain reaction analysis of four domains within the envelope showed that viral subtypes with particular envelope domains segregated to different tissues as defined by the presence or absence of a given type of domain. Collectively, these results show that virus variants in the env gene home to different tissues, presumably because of selection for tissue-specific envelope determinants.

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Publication Date: 1992-06-01 PubMed ID: 1316487PubMed Central: PMC241174DOI: 10.1128/JVI.66.6.3879-3882.1992Google Scholar: Lookup

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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the analysis and understanding of the genomic variation of Equine Infectious Anemia Virus (EIAV) and how its different variants are distributed in diverse tissues of an infected horse.

Research Objective and Methodology

The study aimed to understand the genome variability of EIAV and its distribution within various tissues of a horse infected with a highly virulent variant of the EIAV Wyoming strain. EIAV is a lentivirus that primarily infects the monocyte/macrophage populations of blood and tissues.
Polymerase chain reaction (PCR), a technique used to amplify small segments of DNA, was employed to analyze the EIAV DNA. This was done to discern its distribution and variability across tissues.
PCR primer pairs including long terminal repeat, gag, and pol were used to direct the amplification of EIAV DNA from peripheral blood mononuclear cells and other cells in organs such as the kidney, spleen, liver, lymph node, cerebellum, and periventricular regions of the brain.
An analysis of four domains within the virus’s envelope was initiated to understand the virus subtypes and their segregation across different tissues.

Key Findings and Conclusion

Upon analysis, it was found that different variants of the virus possessing particular envelope domains segregated to different tissues. The segregation was identified by the presence or absence of a given type of domain in different tissues.
This suggests that the variants in the ‘env’ gene of the virus home to different tissues, presumably due to selection for tissue-specific envelope determinants. This indicates complex tissue-specific selection of viral strains during infection.
The results offer a deeper understanding of how EIAV operates and adapts within different tissues, which will be valuable for designing strategies for therapeutic intervention.

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APA

Kim CH, Casey JW. (1992). Genomic variation and segregation of equine infectious anemia virus during acute infection. J Virol, 66(6), 3879-3882. https://doi.org/10.1128/JVI.66.6.3879-3882.1992

Publication

Journal of virology

ISSN: 0022-538X

NlmUniqueID: 0113724

Country: United States

Language: English

Volume: 66

Issue: 6

Pages: 3879-3882

Researcher Affiliations

Kim, C H

Department of Microbiology, Immunology, and Parasitology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.

Casey, J W

MeSH Terms

Animals
Base Sequence
Equine Infectious Anemia / genetics
Genes, env / genetics
Genetic Variation
Horses
Infectious Anemia Virus, Equine / classification
Infectious Anemia Virus, Equine / genetics
Infectious Anemia Virus, Equine / pathogenicity
Molecular Sequence Data
Organ Specificity / genetics
Polymerase Chain Reaction

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Citations

This article has been cited 10 times.

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