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Home / Equine Research Bank / Stand Genomic Sci / Genomic analysis of four strains of Corynebacterium pseudotu...
Standards in genomic sciences2017; 12; 16; doi: 10.1186/s40793-017-0234-6

Genomic analysis of four strains of Corynebacterium pseudotuberculosis bv. Equi isolated from horses showing distinct signs of infection.

Abstract: The genomes of four strains (MB11, MB14, MB30, and MB66) of the species Corynebacterium pseudotuberculosis biovar equi were sequenced on the Ion Torrent PGM platform, completely assembled, and their gene content and structure were analyzed. The strains were isolated from horses with distinct signs of infection, including ulcerative lymphangitis, external abscesses on the chest, or internal abscesses on the liver, kidneys, and lungs. The average size of the genomes was 2.3 Mbp, with 2169 (Strain MB11) to 2235 (Strain MB14) predicted coding sequences (CDSs). An optical map of the MB11 strain generated using the KpnI restriction enzyme showed that the approach used to assemble the genome was satisfactory, producing good alignment between the sequence observed in vitro and that obtained in silico. In the resulting Neighbor-Joining dendrogram, the C. pseudotuberculosis strains sequenced in this study were clustered into a single clade supported by a high bootstrap value. The structural analysis showed that the genomes of the MB11 and MB14 strains were very similar, while the MB30 and MB66 strains had several inverted regions. The observed genomic characteristics were similar to those described for other strains of the same species, despite the number of inversions found. These genomes will serve as a basis for determining the relationship between the genotype of the pathogen and the type of infection that it causes.
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Publication Date: 2017-01-31 PubMed ID: 28163825PubMed Central: PMC5282893DOI: 10.1186/s40793-017-0234-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study performed a full genomic analysis of four strains of harmful bacterium Corynebacterium pseudotuberculosis, which were isolated from differently infected horses. The findings will help understand the relation between the genetic makeup of this pathogen and the type of infection it causes.

Methodology

  • The researchers examined four strains labeled as MB11, MB14, MB30, and MB66 of Corynebacterium pseudotuberculosis biovar Equi, which were extracted from horses showing distinct signs of infection.
  • These strains were isolated from various types of infections in horses including ulcerative lymphangitis, external abscesses on the chest, and internal abscesses on internal organs such as liver, kidneys, and lungs.
  • The genomes of these four strains were sequenced on the Ion Torrent PGM platform and a complete assembly of their genome was created.

Findings

  • The average size of the genomes was found to be 2.3 Million base pairs (Mbp). The total number of predicted coding sequences (CDSs) ranged from 2169 in strain MB11 to 2235 in strain MB14.
  • The approach used for genome assembly was validated using an optical map of the MB11 strain. The map, generated using the KpnI restriction enzyme, showed good alignment between the observed lab sequence and obtained sequence.
  • In a Neighbor-Joining dendrogram, all four strains were clustered into a single group, indicating their genomic similarity.

Conclusion

  • Structural analysis of the genomes revealed that strains MB11 and MB14 shared a high degree of similarity, while the genomes of strains MB30 and MB66 showed several inversions.
  • Despite these inversions, the observed genomic characteristics were similar to those previously described for other strains of the same species.
  • The sequenced and analyzed genomes in this study are considered crucial for future research to determine the relationship between the genetic structure of this pathogen and the type of infection it causes in horses.

Cite This Article

APA
Baraúna RA, Ramos RTJ, Veras AAO, de Sá PHCG, Guimarães LC, das Graças DA, Carneiro AR, Edman JM, Spier SJ, Azevedo V, Silva A. (2017). Genomic analysis of four strains of Corynebacterium pseudotuberculosis bv. Equi isolated from horses showing distinct signs of infection. Stand Genomic Sci, 12, 16. https://doi.org/10.1186/s40793-017-0234-6

Publication

Standards in genomic sciences
ISSN: 1944-3277
NlmUniqueID: 101530505
Country: England
Language: English
Volume: 12
Pages: 16

Researcher Affiliations

Baraúna, Rafael A
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Ramos, Rommel T J
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Veras, Adonney A O
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
de Sá, Pablo H C G
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Guimarães, Luís C
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
das Graças, Diego A
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Carneiro, Adriana R
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Edman, Judy M
  • Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA USA.
Spier, Sharon J
  • Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA USA.
Azevedo, Vasco
  • Federal University of Minas Gerais, Institute of Biological Sciences, Laboratory of Cellular and Molecular Genetics, Belo Horizonte, Brazil.
Silva, Artur
  • Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.

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Citations

This article has been cited 4 times.
  1. Khalid E, Tartor YH, Ammar AM, Abdelaziz R, Mahmmod Y, Abdelkhalek A. Controlling drug-resistant bacteria in Arabian horses: bacteriophage cocktails for treating wound infections. Front Vet Sci 2025;12:1609955.
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  2. Hiller E, Hörz V, Sting R. Corynebacterium pseudotuberculosis: Whole genome sequencing reveals unforeseen and relevant genetic diversity in this pathogen. PLoS One 2024;19(8):e0309282.
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  4. Parise D, Parise MTD, Viana MVC, Muñoz-Bucio AV, Cortés-Pérez YA, Arellano-Reynoso B, Díaz-Aparicio E, Dorella FA, Pereira FL, Carvalho AF, Figueiredo HCP, Ghosh P, Barh D, Gomide ACP, Azevedo VAC. First genome sequencing and comparative analyses of Corynebacterium pseudotuberculosis strains from Mexico. Stand Genomic Sci 2018;13:21.
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