Genomic and antigenic comparison of an equine herpesvirus 1 (EHV 1) isolate from the 1983 Lippizan abortion storm with EHV 1 reference strains.
Abstract: An EHV 1 isolate from the Lippizan Stud at Piber, which caused the abortion and paresis outbreak in 1983, was investigated using 3 known subtype 1 and 2 subtype 2 strains for comparison. Broad-scale restriction enzyme analysis as well as cross-neutralization with hyperimmune sera produced in rabbits were performed, and SDS-PAGE of infected cell proteins was conducted on a limited scale. The Piber isolate was clearly classified as a subtype 1 strain of EHV 1, and showed closest resemblance in its restriction patterns with a British EHV 1 strain, which originated from an outbreak with paretic symptoms. A second Piber isolate from the same outbreak examined to limited extent only was practically indistinguishable from the first one, as could have been expected. A thoroughly controlled systematic vaccination program with existing commercial vaccines against EHV 1 should protect the endangered Lippizan horses population against the abortigenic and less certainly against the paretic syndromes caused by this virus. According to data presented, a protection against respiratory disease is less probable.
Publication Date: 1986-04-01 PubMed ID: 3012290
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study explored an outbreak of equine herpesvirus 1 (EHV 1) at Lippizan Stud in 1983, comparing it with reference strains of the virus to better understand its characteristics and implications for future vaccinations.
Research on EHV 1 at Lippizan Stud
- The researchers investigated an EHV 1 isolate from the 1983 outbreak at Lippizan Stud. This outbreak led to abortion and paresis (partial paralysis) in horses.
- They compared the outbreak strain with 3 known subtype 1 EHV 1 strains and 2 known subtype 2 strains.
Methods Used in the Study
- The team performed a broad-scale restriction enzyme analysis to study the genetic makeup of the virus, and carried out cross-neutralization tests with hyperimmune sera produced in rabbits to understand the immune response to the virus.
- Infected cell proteins were evaluated through SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis), a common technique used in biochemistry and molecular biology to separate proteins based on their electrophoretic mobility.
- Only limited testing was conducted on a second isolate from the same outbreak.
Findings and Recommendations
- The Piber isolate from Lippizan Stud was identified as a subtype 1 strain of EHV 1. Its genetic patterns showed the most resemblance to a British EHV 1 strain, which also caused an outbreak with paretic symptoms.
- From the limited testing done, the second isolate appeared to be almost indistinguishable from the first one, which was expected.
- A well-controlled systematic vaccination program with available commercial vaccines against EHV 1 should protect the Lippizan horse population against the specific abortigenic (causing abortion) strain, with less certainty about protection against the paretic strain caused by this virus.
- The data suggests that protection against respiratory disease caused by the EHV 1 virus is less probable, indicating a potential gap in the existing vaccine’s ability to cover all manifestations of the virus.
Cite This Article
APA
Engels M, Nowotny N, Metzler AE, Wyler R, Bürki F.
(1986).
Genomic and antigenic comparison of an equine herpesvirus 1 (EHV 1) isolate from the 1983 Lippizan abortion storm with EHV 1 reference strains.
Microbiologica, 9(2), 221-234.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antigens, Viral / analysis
- Cell Line
- Cells, Cultured
- DNA Restriction Enzymes
- DNA, Viral / genetics
- Female
- Herpesviridae / genetics
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Equid / genetics
- Herpesvirus 1, Equid / immunology
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases / microbiology
- Horses
- Kidney
- Molecular Weight
- Pregnancy
- Skin
- Viral Proteins / isolation & purification
Citations
This article has been cited 3 times.- Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Roberts HC, Padalino B, Pasquali P, Spoolder H, Ståhl K, Calvo AV, Viltrop A, Winckler C, Carvelli A, Paillot R, Broglia A, Kohnle L, Baldinelli F, Van der Stede Y. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus-1. EFSA J 2022 Jan;20(1):e07036.
- Vögtlin A, Fraefel C, Albini S, Leutenegger CM, Schraner E, Spiess B, Lutz H, Ackermann M. Quantification of feline herpesvirus 1 DNA in ocular fluid samples of clinically diseased cats by real-time TaqMan PCR. J Clin Microbiol 2002 Feb;40(2):519-23.
- Bonass WA, Hudson WA, Elton DM, Killington RA, Halliburton IW. Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates. Arch Virol 1994;134(1-2):169-78.
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