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PloS one2012; 7(1); e29953; doi: 10.1371/journal.pone.0029953

Genomic characterization of the Taylorella genus.

Abstract: The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the recently-sequenced T. equigenitalis genome. The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS). While 212 CDSs were T. asinigenitalis-specific, 1,322 had orthologs in T. equigenitalis. Two hundred and thirty-four T. equigenitalis CDSs had no orthologs in T. asinigenitalis. Analysis of the basic nutrition metabolism of both Taylorella species showed that malate, glutamate and alpha-ketoglutarate may be their main carbon and energy sources. For both species, we identified four different secretion systems and several proteins potentially involved in binding and colonization of host cells, suggesting a strong potential for interaction with their host. T. equigenitalis seems better-equipped than T. asinigenitalis in terms of virulence since we identified numerous proteins potentially involved in pathogenicity, including hemagluttinin-related proteins, a type IV secretion system, TonB-dependent lactoferrin and transferrin receptors, and YadA and Hep_Hag domains containing proteins. This is the first molecular characterization of Taylorella genus members, and the first molecular identification of factors potentially involved in T. asinigenitalis and T. equigenitalis pathogenicity and host colonization. This study facilitates a genetic understanding of growth phenotypes, animal host preference and pathogenic capacity, paving the way for future functional investigations into this largely unknown genus.
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Publication Date: 2012-01-03 PubMed ID: 22235352PubMed Central: PMC3250509DOI: 10.1371/journal.pone.0029953Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article reports on the first genome sequence of Taylorella asinigenitalis, comparing it with the genome of Taylorella equigenitalis. Through this comparison, the article explores the genetic understanding of growth phenotypes, animal host preference and pathogenicity in this largely unknown genus.

Genome Analysis of the Taylorella Genus

The Taylorella genus has two species: T. equigenitalis and T. asinigenitalis. In this research, the T. asinigenitalis genome was sequenced and analyzed in relation to the already sequenced T. equigenitalis genome.

  • The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS).
  • These sequences were compared to T. equigenitalis and it was found that 212 CDS were specific to T. asinigenitalis, while 1,322 had orthologs in T. equigenitalis. Furthermore, 234 T. equigenitalis CDS had no matching sequences in T. asinigenitalis.

Comparison of Basic Nutrition Metabolism

The basic nutrition metabolism of both species was analyzed revealing valuable insights.

  • It was found that malate, glutamate and alpha-ketoglutarate may be the main sources of carbon and energy for both species.

Analysis of Secretion Systems and Potential Interaction with Host

The study identified various secretion systems and proteins in both species that could play a significant role in binding and host cell colonization.

  • In both species, four different secretion systems were identified that suggest a high potential for host interaction.
  • Several proteins potentially involved in such binding and colonization were also identified, underlining the intimate relationship they might have with their hosts.

Differential Virulence and Host Interaction

The analysis showed differences in potential virulence and host interaction between T. equigenitalis and T. asinigenitalis.

  • T. equigenitalis seems to be better equipped than T. asinigenitalis in terms of virulence. There were numerous proteins identified that could potentially be involved in pathogenicity.

Contributions of the Research

This study is the first molecular characterization of species in the Taylorella genus, and also the first to identify factors potentially involved in the pathogenicity and host colonization of T. asinigenitalis and T. equigenitalis. This significant piece of research further enhances scientific understanding about this largely unknown genus. It could also pave the way for future functional investigations into how these species grow, their animal host preferences and their capacity for pathogenicity.

Cite This Article

APA
Hébert L, Moumen B, Pons N, Duquesne F, Breuil MF, Goux D, Batto JM, Laugier C, Renault P, Petry S. (2012). Genomic characterization of the Taylorella genus. PLoS One, 7(1), e29953. https://doi.org/10.1371/journal.pone.0029953

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 7
Issue: 1
Pages: e29953

Researcher Affiliations

Hébert, Laurent
  • ANSES, Dozulé Laboratory for Equine Diseases, Dozulé, France. laurent.hebert@anses.fr
Moumen, Bouziane
    Pons, Nicolas
      Duquesne, Fabien
        Breuil, Marie-France
          Goux, Didier
            Batto, Jean-Michel
              Laugier, Claire
                Renault, Pierre
                  Petry, Sandrine

                    MeSH Terms

                    • Bacterial Proteins / genetics
                    • Bacterial Proteins / metabolism
                    • Burkholderia / classification
                    • Burkholderia / genetics
                    • Carbon / metabolism
                    • Genome, Bacterial / genetics
                    • Genomics / methods
                    • Oxidative Stress / genetics
                    • Phylogeny
                    • Sequence Alignment
                    • Species Specificity
                    • Taylorella / genetics
                    • Taylorella / metabolism
                    • Virulence Factors / genetics

                    Conflict of Interest Statement

                    Competing Interests: The authors have declared that no competing interests exist.

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