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Home / Equine Research Bank / PLoS One / Genomic characterization of the Taylorella genus.
PloS one2012; 7(1); e29953; doi: 10.1371/journal.pone.0029953

Genomic characterization of the Taylorella genus.

Abstract: The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the recently-sequenced T. equigenitalis genome. The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS). While 212 CDSs were T. asinigenitalis-specific, 1,322 had orthologs in T. equigenitalis. Two hundred and thirty-four T. equigenitalis CDSs had no orthologs in T. asinigenitalis. Analysis of the basic nutrition metabolism of both Taylorella species showed that malate, glutamate and alpha-ketoglutarate may be their main carbon and energy sources. For both species, we identified four different secretion systems and several proteins potentially involved in binding and colonization of host cells, suggesting a strong potential for interaction with their host. T. equigenitalis seems better-equipped than T. asinigenitalis in terms of virulence since we identified numerous proteins potentially involved in pathogenicity, including hemagluttinin-related proteins, a type IV secretion system, TonB-dependent lactoferrin and transferrin receptors, and YadA and Hep_Hag domains containing proteins. This is the first molecular characterization of Taylorella genus members, and the first molecular identification of factors potentially involved in T. asinigenitalis and T. equigenitalis pathogenicity and host colonization. This study facilitates a genetic understanding of growth phenotypes, animal host preference and pathogenic capacity, paving the way for future functional investigations into this largely unknown genus.
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Publication Date: 2012-01-03 PubMed ID: 22235352PubMed Central: PMC3250509DOI: 10.1371/journal.pone.0029953Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article reports on the first genome sequence of Taylorella asinigenitalis, comparing it with the genome of Taylorella equigenitalis. Through this comparison, the article explores the genetic understanding of growth phenotypes, animal host preference and pathogenicity in this largely unknown genus.

Genome Analysis of the Taylorella Genus

The Taylorella genus has two species: T. equigenitalis and T. asinigenitalis. In this research, the T. asinigenitalis genome was sequenced and analyzed in relation to the already sequenced T. equigenitalis genome.

  • The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS).
  • These sequences were compared to T. equigenitalis and it was found that 212 CDS were specific to T. asinigenitalis, while 1,322 had orthologs in T. equigenitalis. Furthermore, 234 T. equigenitalis CDS had no matching sequences in T. asinigenitalis.

Comparison of Basic Nutrition Metabolism

The basic nutrition metabolism of both species was analyzed revealing valuable insights.

  • It was found that malate, glutamate and alpha-ketoglutarate may be the main sources of carbon and energy for both species.

Analysis of Secretion Systems and Potential Interaction with Host

The study identified various secretion systems and proteins in both species that could play a significant role in binding and host cell colonization.

  • In both species, four different secretion systems were identified that suggest a high potential for host interaction.
  • Several proteins potentially involved in such binding and colonization were also identified, underlining the intimate relationship they might have with their hosts.

Differential Virulence and Host Interaction

The analysis showed differences in potential virulence and host interaction between T. equigenitalis and T. asinigenitalis.

  • T. equigenitalis seems to be better equipped than T. asinigenitalis in terms of virulence. There were numerous proteins identified that could potentially be involved in pathogenicity.

Contributions of the Research

This study is the first molecular characterization of species in the Taylorella genus, and also the first to identify factors potentially involved in the pathogenicity and host colonization of T. asinigenitalis and T. equigenitalis. This significant piece of research further enhances scientific understanding about this largely unknown genus. It could also pave the way for future functional investigations into how these species grow, their animal host preferences and their capacity for pathogenicity.

Cite This Article

APA
Hébert L, Moumen B, Pons N, Duquesne F, Breuil MF, Goux D, Batto JM, Laugier C, Renault P, Petry S. (2012). Genomic characterization of the Taylorella genus. PLoS One, 7(1), e29953. https://doi.org/10.1371/journal.pone.0029953

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 7
Issue: 1
Pages: e29953

Researcher Affiliations

Hébert, Laurent
  • ANSES, Dozulé Laboratory for Equine Diseases, Dozulé, France. laurent.hebert@anses.fr
Moumen, Bouziane
    Pons, Nicolas
      Duquesne, Fabien
        Breuil, Marie-France
          Goux, Didier
            Batto, Jean-Michel
              Laugier, Claire
                Renault, Pierre
                  Petry, Sandrine

                    MeSH Terms

                    • Bacterial Proteins / genetics
                    • Bacterial Proteins / metabolism
                    • Burkholderia / classification
                    • Burkholderia / genetics
                    • Carbon / metabolism
                    • Genome, Bacterial / genetics
                    • Genomics / methods
                    • Oxidative Stress / genetics
                    • Phylogeny
                    • Sequence Alignment
                    • Species Specificity
                    • Taylorella / genetics
                    • Taylorella / metabolism
                    • Virulence Factors / genetics

                    Conflict of Interest Statement

                    Competing Interests: The authors have declared that no competing interests exist.

                    References

                    This article includes 83 references
                    1. Sugimoto C, Isayama Y, Sakazaki R, Kuramochi S. Transfer of Haemophilus equigenitalis Taylor et al. 1978 to the genus Taylorella gen. nov. as Taylorella equigenitalis comb. nov.. Curr Microbiol 1983;9:155–162.
                    2. Crowhurst RC. Genital infection in mares.. Vet Rec 1977;100:476.
                      pubmed: 878259
                    3. Timoney PJ, Ward J, Kelly P. A contagious genital infection of mares.. Vet Rec 1977;101:103.
                      pubmed: 906223
                    4. Timoney PJ. Contagious equine metritis: An insidious threat to the U.S. horse breeding industry.. J Anim Sci 2011. pp. jas.2010–3368.
                      pubmed: 20889687
                    5. Matsuda M, Moore JE. Recent advances in molecular epidemiology and detection of Taylorella equigenitalis associated with contagious equine metritis (CEM).. Vet Microbiol 2003;97:111–122.
                      pubmed: 14637043
                    6. Lindmark DG, Jarroll EL, Timoney PJ, Shin SJ. Energy metabolism of the contagious equine metritis bacterium.. Infect Immun 1982;36:531–534.
                      pmc: PMC351260pubmed: 7085071
                    7. Hitchcock PJ, Brown TM, Corwin D, Hayes SF, Olszewski A. Morphology of three strains of contagious equine metritis organism.. Infect Immun 1985;48:94–108.
                      pmc: PMC261920pubmed: 3838532
                    8. Kanemaru T, Kamada M, Wada R, Anzai T, Kumanomido T. Electron microscopic observation of Taylorella equigenitalis with pili in vivo.. J Vet Med Sci 1992;54:345–347.
                      pubmed: 1351406
                    9. Bertram TA, Coignoul FL, Jensen AE. Phagocytosis and intracellular killing of the contagious equine metritis organism by equine neutrophils in serum.. Infect Immun 1982;37:1241–1247.
                      pmc: PMC347671pubmed: 7129636
                    10. Bleumink-Pluym N, ter Laak E, Houwers D, van der Zeijst B. Differences between Taylorella equigenitalis strains in their invasion of and replication in cultured cells.. Clin Diagn Lab Immunol 1996;3:47–50.
                      pmc: PMC170246pubmed: 8770503
                    11. Katz JB, Evans LE, Hutto DL, Schroeder-Tucker LC, Carew AM. Clinical, bacteriologic, serologic, and pathologic features of infections with atypical Taylorella equigenitalis in mares.. J Am Vet Med Assoc 2000;216:1945–1948.
                      pubmed: 10863594
                    12. Jang S, Donahue J, Arata A, Goris J, Hansen L. Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus).. Int J Syst Evol Microbiol 2001;51:971–976.
                      pubmed: 11411723
                    13. Breuil MF, Duquesne F, Laugier C, Petry S. Phenotypic and 16S ribosomal RNA gene diversity of Taylorella asinigenitalis strains isolated between 1995 and 2008.. Vet Microbiol 2011;148:260–266.
                      pubmed: 21067874
                    14. Hébert L, Moumen B, Duquesne F, Breuil M-F, Laugier C. Genome sequence of Taylorella equigenitalis MCE9, the causative agent of contagious equine metritis.. J Bacteriol 2011;193:1785.
                      pmc: PMC3067654pubmed: 21278298
                    15. Pons N, Batto JM, Ehrlich SD, Renault P. Development of software facilities to characterize regulatory binding motifs and application to Streptococcaceae.. J Mol Microb Biotech 2008;14:67–73.
                      pubmed: 17957112
                    16. Willems A, Gilhaus H, Beer W, Mietke H, Gelderblom HR. Brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus).. Int J Syst Evol Microbiol 2002;52:179–186.
                      pubmed: 11837301
                    17. Temple LM, Miyamoto DM, Mehta M, Capitini CM, Von Stetina S. Identification and characterization of two Bordetella avium gene products required for hemagglutination.. Infect Immun 2010;78:2370–2376.
                      pmc: PMC2876549pubmed: 20351141
                    18. Bunikis I, Denker K, Östberg Y, Andersen C, Benz R. An RND-type efflux system in Borrelia burgdorferi is involved in virulence and resistance to antimicrobial compounds.. PLoS Pathog 2008;4:e1000009.
                      pmc: PMC2279261pubmed: 18389081
                    19. Backert S, Meyer TF. Type IV secretion systems and their effectors in bacterial pathogenesis.. Curr Opin Microbiol 2006;9:207–217.
                      pubmed: 16529981
                    20. Sisto A, Cipriani M, Morea M, Lonigro S, Valerio F. An Rhs-like genetic element is involved in bacteriocin production by Pseudomonas savastanoi pv. savastanoi.. Antonie van Leeuwenhoek 2010;98:505–517.
                      pubmed: 20563849
                    21. Labrie SJ, Samson JE, Moineau S. Bacteriophage resistance mechanisms.. Nat Rev Micro 2010;8:317–327.
                      pubmed: 20348932
                    22. Jansen R, Embden JDAv, Gaastra W, Schouls LM. Identification of genes that are associated with DNA repeats in prokaryotes.. Mol Microbiol 2002;43:1565–1575.
                      pubmed: 11952905
                    23. Kobayashi I. Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution.. Nucleic Acids Res 2001;29:3742–3756.
                      pmc: PMC55917pubmed: 11557807
                    24. Vinogradov E, MacLean LL, Brooks BW, Lutze-Wallace C, Perry MB. Structure of the O-polysaccharide of the lipopolysaccharide produced by Taylorella asinigenitalis type strain (ATCC 700933).. Biochem Cell Biol 2008;86:278–284.
                      pubmed: 18523489
                    25. Vinogradov E, MacLean LL, Brooks BW, Lutze-Wallace C, Perry MB. The structure of the polysaccharide of the lipopolysaccharide produced by Taylorella equigenitalis type strain (ATCC 35865).. Carbohyd Res 2008;343:3079–3084.
                      pubmed: 18950750
                    26. Brooks BW, Lutze-Wallace CL, Maclean LL, Vinogradov E, Perry MB. Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies.. Can J Vet Res 2010;74:18–24.
                      pmc: PMC2801306pubmed: 20357953
                    27. Alm RA, Ling L-SL, Moir DT, King BL, Brown ED. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.. Nature 1999;397:176–180.
                      pubmed: 9923682
                    28. Chinen A, Uchiyama I, Kobayashi I. Comparison between Pyrococcus horikoshii and Pyrococcus abyssi genome sequences reveals linkage of restriction-modification genes with large genome polymorphisms.. Gene 2000;259:109–121.
                      pubmed: 11163968
                    29. McGlynn P, Lloyd RG. Genome stability and the processing of damaged replication forks by RecG.. Trends Genet 2002;18:413–419.
                      pubmed: 12142010
                    30. Tiyawisutsri R, Holden M, Tumapa S, Rengpipat S, Clarke S. Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis.. BMC Microbiol 2007;7:19.
                      pmc: PMC1847439pubmed: 17362501
                    31. Arioli S, Roncada P, Salzano AM, Deriu F, Corona S. The relevance of carbon dioxide metabolism in Streptococcus thermophilus.. Microbiology 2009;155:1953–1965.
                      pubmed: 19372152
                    32. Lolkema JS, Poolman B, Konings WN. Bacterial solute uptake and efflux systems.. Curr Opin Microbiol 1998;1:248–253.
                      pubmed: 10066480
                    33. Antoine R, Huvent I, Chemlal K, Deray I, Raze D. The periplasmic binding protein of a tripartite tricarboxylate transporter is involved in signal transduction.. J Mol Biol 2005;351:799–809.
                      pubmed: 16045930
                    34. Davidson AL, Dassa E, Orelle C, Chen J. Structure, function, and evolution of bacterial ATP-binding cassette systems.. Microbiol Mol Biol Rev 2008;72:317–364.
                      pmc: PMC2415747pubmed: 18535149
                    35. Mulligan C, Fischer M, Thomas GH. Tripartite ATP-independent periplasmic (TRAP) transporters in bacteria and archaea.. FEMS Microbiology Reviews 2010;35:68–86.
                      pubmed: 20584082
                    36. Winnen B, Hvorup RN, Saier MH. The tripartite tricarboxylate transporter (TTT) family.. Res Microbiol 2003;154:457–465.
                      pubmed: 14499931
                    37. Boël G, Mijakovic I, Mazé A, Poncet S, Taha MK. Transcription regulators potentially controlled by HPr kinase/phosphorylase in Gram-negative bacteria.. J Mol Microb Biotech 2003;5:206–215.
                      pubmed: 12867744
                    38. Jin RZ, Lin ECC. An inducible phosphoenolpyruvate: dihydroxyacetone phosphotransferase system in Escherichia coli.. J Gen Microbiol 1984;130:83–88.
                      pubmed: 6368745
                    39. Deutscher J, Francke C, Postma PW. How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.. Microbiol Mol Biol Rev 2006;70:939–1031.
                      pmc: PMC1698508pubmed: 17158705
                    40. Pitcher RS, Watmough NJ. The bacterial cytochrome cbb3 oxidases.. Biochem Biophys Acta 2004;1655:388–399.
                      pubmed: 15100055
                    41. Sasindran SJ, Saikolappan S, Dhandayuthapani S. Methionine sulfoxide reductases and virulence of bacterial pathogens.. Future Microbiol 2007;2:619–630.
                      pubmed: 18041903
                    42. Jehl M-A, Arnold R, Rattei T. Effective-a database of predicted secreted bacterial proteins.. Nucleic Acids Res 2011;39:D591–D595.
                      pmc: PMC3013723pubmed: 21071416
                    43. Tseng T-T, Tyler B, Setubal J. Protein secretion systems in bacterial-host associations, and their description in the Gene Ontology.. BMC Microbiol 2009;9:S2.
                      pmc: PMC2654662pubmed: 19278550
                    44. Preston GM, Haubold B, Rainey PB. Bacterial genomics and adaptation to life on plants: implications for the evolution of pathogenicity and symbiosis.. Curr Opin Microbiol 1998;1:589–597.
                      pubmed: 10066526
                    45. Sandkvist M. Biology of type II secretion.. Mol Microbiol 2001;40:271–283.
                      pubmed: 11309111
                    46. Stathopoulos C, Hendrixson DR, Thanassi DG, Hultgren SJ, Geme Joseph W St, Iii. Secretion of virulence determinants by the general secretory pathway in Gram-negative pathogens: an evolving story.. Microbes Infect 2000;2:1061–1072.
                      pubmed: 10967286
                    47. Tomich M, Planet PJ, Figurski DH. The tad locus: postcards from the widespread colonization island.. Nat Rev Microbiol 2007;5:363–375.
                      pubmed: 17435791
                    48. Motherway MOC, Zomer A, Leahy SC, Reunanen J, Bottacini F. Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor.. Proc Natl Acad Sci USA 2011.
                      pmc: PMC3131351pubmed: 21690406
                    49. Rêgo AT, Chandran V, Waksman G. Two-step and one-step secretion mechanisms in Gram-negative bacteria: contrasting the type IV secretion system and the chaperone-usher pathway of pilus biogenesis.. Biochem J 2010;425:475–488.
                      pubmed: 20070257
                    50. Wallden K, Rivera-Calzada A, Waksman G. Microreview: Type IV secretion systems: versatility and diversity in function.. Cell Microbiol 2010;12:1203–1212.
                      pmc: PMC3070162pubmed: 20642798
                    51. Tegtmeyer N, Wessler S, Backert S. Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis.. FEBS J 2011;278:1190–1202.
                      pmc: PMC3070773pubmed: 21352489
                    52. Pukatzki S, Ma AT, Sturtevant D, Krastins B, Sarracino D. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system.. Proc Natl Acad Sci USA 2006;103:1528–1533.
                      pmc: PMC1345711pubmed: 16432199
                    53. Bönemann G, Pietrosiuk A, Mogk A. Tubules and donuts: a type VI secretion story.. Mol Microbiol 2010;76:815–821.
                      pubmed: 20444095
                    54. Jani AJ, Cotter PA. Type VI secretion: not just for pathogenesis anymore.. Cell Host Microbe 2010;8:2–6.
                      pmc: PMC2913581pubmed: 20638635
                    55. Pukatzki S, McAuley SB, Miyata ST. The type VI secretion system: translocation of effectors and effector-domains.. Curr Opin Microbiol 2009;12:11–17.
                      pubmed: 19162533
                    56. van de Guchte M, Penaud S, Grimaldi C, Barbe V, Bryson K. The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution.. Proc Natl Acad Sci USA 2006;103:9274–9279.
                      pmc: PMC1482600pubmed: 16754859
                    57. Willems A, Gilhaus H, Beer W, Mietke H, Gelderblom HR. Bacterial Adhesion: Springer Netherlands; 2011. Adhesion Mechanisms of Staphylococci.. pp. 105–123.
                    58. Yang J, Chen L, Sun L, Yu J, Jin Q. VFDB 2008 release: an enhanced web-based resource for comparative pathogenomics.. Nucleic Acids Res 2008;36:D539–D542.
                      pmc: PMC2238871pubmed: 17984080
                    59. Pan X, Lührmann A, Satoh A, Laskowski-Arce MA, Roy CR. Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors.. Science 2008;320:1651–1654.
                      pmc: PMC2514061pubmed: 18566289
                    60. Penz T, Horn M, Schmitz-Esser S. The genome of the amoeba symbiont “Candidatus Amoebophilus asiaticus” encodes an afp-like prophage possibly used for protein secretion.. Virulence 2010;1:541–545.
                      pubmed: 21178499
                    61. Al-Khodor S, Price CT, Kalia A, Abu Kwaik Y. Functional diversity of ankyrin repeats in microbial proteins.. Trends Microbiol 2009;18:132–139.
                      pmc: PMC2834824pubmed: 19962898
                    62. Bella J, Hindle K, McEwan P, Lovell S. The leucine-rich repeat structure.. Cell Mol Life Sci 2008;65:2307–2333.
                      pmc: PMC11131621pubmed: 18408889
                    63. Beddek A, Schryvers A. The lactoferrin receptor complex in gram negative bacteria.. BioMetals 2010;23:377–386.
                      pubmed: 20155302
                    64. Garduno RA, Chong A, Nasrallah GK, Allan DS. The Legionella pneumophila chaperonin - an unusual multifunctional protein in unusual locations.. Front Microbiol 2011;2.
                      pmc: PMC3114179pubmed: 21713066
                    65. Joseph B, Goebel W. Life of Listeria monocytogenes in the host cells' cytosol.. Microbes Infect 2007;9:1188–1195.
                      pubmed: 17719818
                    66. Eisen J, Heidelberg J, White O, Salzberg S. Evidence for symmetric chromosomal inversions around the replication origin in bacteria.. Genome Biol 1: 2000. research0011.0011 - research0011.0019.
                      pmc: PMC16139pubmed: 11178265
                    67. Li Y, Zheng H, Liu Y, Jiang Y, Xin J. The complete genome sequence of Mycoplasma bovis strain Hubei-1.. PLoS ONE 2011;6:e20999.
                      pmc: PMC3120828pubmed: 21731639
                    68. Maruyama F, Kobata M, Kurokawa K, Nishida K, Sakurai A. Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content.. BMC Genomics 2009;10:358.
                      pmc: PMC2907686pubmed: 19656368
                    69. Archer C, Kim J, Jeong H, Park J, Vickers C. The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli.. BMC Genomics 2011;12:9.
                      pmc: PMC3032704pubmed: 21208457
                    70. Duquesne F, Pronost S, Laugier C, Petry S. Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction.. Res Vet Sci 2007;82:47–49.
                      pubmed: 16806331
                    71. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS. Genome sequencing in microfabricated high-density picolitre reactors.. Nature 2005;437:376–380.
                      pmc: PMC1464427pubmed: 16056220
                    72. Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J. Accurate whole human genome sequencing using reversible terminator chemistry.. Nature 2008;456:53–59.
                      pmc: PMC2581791pubmed: 18987734
                    73. Chevreux B, Wetter T, Suhai S, Giegerich R. Genome sequence assembly using trace signals and additional sequence information.. 1999. pp. 45–56. (ed), Proceedings of the German Conference on Bioinformatics GBF-Braunschweig and University of Bielefeld, Bielefeld, Germany.
                    74. Gordon D, Abajian C, Green P. Consed: a graphical tool for sequence finishing.. Genome Res 1998;8:195–202.
                      pubmed: 9521923
                    75. Kanehisa M. A database for post-genome analysis.. Trends Genet 1997;13:375–376.
                      pubmed: 9287494
                    76. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T. The RAST server: Rapid annotations using subsystems technology.. BMC Genomics 2008;9:75.
                      pmc: PMC2265698pubmed: 18261238
                    77. Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.. Nucleic Acids Res 1997;25:955–964.
                      pmc: PMC146525pubmed: 9023104
                    78. Lagesen K, Hallin P, Rødland EA, Stærfeldt H-H, Rognes T. RNAmmer: consistent and rapid annotation of ribosomal RNA genes.. Nucleic Acids Res 2007;35:3100–3108.
                      pmc: PMC1888812pubmed: 17452365
                    79. Grissa I, Vergnaud G, Pourcel C. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats.. Nucleic Acids Res 2007;35:W52–W57.
                      pmc: PMC1933234pubmed: 17537822
                    80. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res 1994;22:4673–4680.
                      pmc: PMC308517pubmed: 7984417
                    81. Tamura K, Peterson D, Peterson N, Stecher G, Nei M. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.. Mol Biol Evol 2011.
                      pmc: PMC3203626pubmed: 21546353
                    82. Abbott JC, Aanensen DM, Rutherford K, Butcher S, Spratt BG. WebACT–an online companion for the Artemis Comparison Tool.. Bioinformatics 2005;21:3665–3666.
                      pubmed: 16076890
                    83. Carver TJ, Rutherford KM, Berriman M, Rajandream M, Barrell BG. ACT: the Artemis comparison tool.. Bioinformatics 2005;21:3422–3423.
                      pubmed: 15976072

                    Citations

                    This article has been cited 8 times.
                    1. Hrala M, Andrla P, Bosák J, Fedrová P, Mugutdinov A, Karpíšková R, Nedbalcová K, Raichová J, Faldyna M, Hořín P, Šmajs D. Whole genome sequences of nine Taylorella equigenitalis strains isolated in the Czech Republic between 1982-2021: Molecular dating suggests a common ancestor at the time of Roman Empire. PLoS One 2025;20(1):e0315946.
                      doi: 10.1371/journal.pone.0315946pubmed: 39752466google scholar: lookup
                    2. Calder A, Menkiti CJ, Çağdaş A, Lisboa Santos J, Streich R, Wong A, Avini AH, Bojang E, Yogamanoharan K, Sivanesan N, Ali B, Ashrafi M, Issa A, Kaur T, Latif A, Mohamed HAS, Maqsood A, Tamang L, Swager E, Stringer AJ, Snyder LAS. Virulence genes and previously unexplored gene clusters in four commensal Neisseria spp. isolated from the human throat expand the neisserial gene repertoire. Microb Genom 2020 Sep;6(9).
                      doi: 10.1099/mgen.0.000423pubmed: 32845827google scholar: lookup
                    3. May CE, Guthrie AJ, Schulman ML. Direct culture-independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses. J Vet Diagn Invest 2019 Sep;31(5):792-794.
                      doi: 10.1177/1040638719871089pubmed: 31423914google scholar: lookup
                    4. Allombert J, Vianney A, Laugier C, Petry S, Hébert L. Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii. BMC Microbiol 2014 Mar 19;14:69.
                      doi: 10.1186/1471-2180-14-69pubmed: 24641089google scholar: lookup
                    5. Whiteson KL, Hernandez D, Lazarevic V, Gaia N, Farinelli L, François P, Pilo P, Frey J, Schrenzel J. A genomic perspective on a new bacterial genus and species from the Alcaligenaceae family, Basilea psittacipulmonis. BMC Genomics 2014 Mar 1;15:169.
                      doi: 10.1186/1471-2164-15-169pubmed: 24581117google scholar: lookup
                    6. Ghosh W, Alam M, Roy C, Pyne P, George A, Chakraborty R, Majumder S, Agarwal A, Chakraborty S, Majumdar S, Gupta SK. Genome implosion elicits host-confinement in Alcaligenaceae: evidence from the comparative genomics of Tetrathiobacter kashmirensis, a pathogen in the making. PLoS One 2013;8(5):e64856.
                      doi: 10.1371/journal.pone.0064856pubmed: 23741407google scholar: lookup
                    7. Motta MC, Martins AC, de Souza SS, Catta-Preta CM, Silva R, Klein CC, de Almeida LG, de Lima Cunha O, Ciapina LP, Brocchi M, Colabardini AC, de Araujo Lima B, Machado CR, de Almeida Soares CM, Probst CM, de Menezes CB, Thompson CE, Bartholomeu DC, Gradia DF, Pavoni DP, Grisard EC, Fantinatti-Garboggini F, Marchini FK, Rodrigues-Luiz GF, Wagner G, Goldman GH, Fietto JL, Elias MC, Goldman MH, Sagot MF, Pereira M, Stoco PH, de Mendonça-Neto RP, Teixeira SM, Maciel TE, de Oliveira Mendes TA, Ürményi TP, de Souza W, Schenkman S, de Vasconcelos AT. Predicting the proteins of Angomonas deanei, Strigomonas culicis and their respective endosymbionts reveals new aspects of the trypanosomatidae family. PLoS One 2013;8(4):e60209.
                      doi: 10.1371/journal.pone.0060209pubmed: 23560078google scholar: lookup
                    8. Hara Y, Hayashi K, Nakajima T, Kagawa S, Tazumi A, Moore JE, Matsuda M. Molecular identification and characterization of clustered regularly interspaced short palindromic repeat (CRISPR) gene cluster in Taylorella equigenitalis. Folia Microbiol (Praha) 2013 Sep;58(5):375-84.
                      doi: 10.1007/s12223-012-0217-3pubmed: 23275249google scholar: lookup
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