Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples.
Abstract: Several attempts have been made to categorize equid- and bovid-specific bovine papillomavirus 1 (BPV1) isolates based on sequence tags. This study includes newly determined sequence information from 33 BPV1 isolates of equine, asinine and bovine origin and investigates sequence bias due to host species. Twenty of the viral genomes were sequenced over their entire length and a further thirteen were sequenced, including flanking sequences, at two specific sites, the LCR and the E5 ORF. Alignment and analyses of the sequences did not reveal statistically significant site differences between the sequences of bovine and equid origin. None of the proposed sites of divergence noted by other authors demonstrated significant species-specific characteristics. Our results suggest that BPV1 is shared between equine, asinine and bovine host species, and that viral transfer between bovines and equids is a repeated and ongoing phenomenon.
Copyright © 2017 Elsevier B.V. All rights reserved.
Publication Date: 2017-11-04 PubMed ID: 29113823DOI: 10.1016/j.virusres.2017.11.005Google Scholar: Lookup
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- Journal Article
Summary
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The research article discusses an analysis of the genetic sequences of bovine papillomavirus 1 (BPV1) from horses, donkeys, and cows. The study concluded that this virus is shared among these species and its transfer between them is continuous and recurring.
Objective of the Study
- The research aims to examine genetic differences in bovine papillomavirus 1 (BPV1) isolates from three different animals: bovines (cattle), equines (horses), and asinines (donkeys). It does so by comparing the full or partial sequences of 33 viral genomes to determine if there are significant genetic variations based on the host species.
Methods of the Study
- Scientists obtained BPV1 isolates from affected tissues of the three animal groups and performed genetic sequencing on these isolates.
- The genomic sequences of 20 isolates were fully determined, while for another 13 isolates, only two specific sections of the viral genome, the Long Control Region (LCR) and the E5 Open Reading Frame (ORF), were analyzed.
- The researchers then aligned and analyzed these genetic sequences to look for differences or biases influenced by the host species.
Results of the Study
- Analysis of the nucleotide sequences did not show statistically significant differences between bovine and equine isolates.
- Furthermore, contrary to previous studies, this research did not find any specific sites of divergence between the two that could be identified as species-specific characteristics.
Conclusions and Implications
- The results suggest that BPV1 virus is common between bovine, equine, and asinine species.
- Additionally, the transmission of the virus between cattle and horses seems to be an ongoing and recurrent event. This leads to the inference that these species might be playing a role in the spread and maintenance of the disease.
- This heightened understanding of BPV1’s genetic similarities across species can aid in designing more effective strategies for controlling the spread of the virus and disease management.
Cite This Article
APA
Koch C, Ramsauer AS, Drögemüller M, Ackermann M, Gerber V, Tobler K.
(2017).
Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples.
Virus Res, 244, 6-12.
https://doi.org/10.1016/j.virusres.2017.11.005 Publication
Researcher Affiliations
- Swiss Institute of Equine Medicine, Vetsuisse Faculty Berne, University of Berne, and ALP-Haras, Länggasstrasse 124, Postfach 8466, CH-3001 Berne, Switzerland. Electronic address: christoph.koch@vetsuisse.unibe.ch.
- Institute of Virology, Vetsuisse Faculty Zurich, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland.
- Institute of Genetics, Vetsuisse Faculty Berne, University of Berne, Bremgartenstrasse 109a, Postfach 8466, CH-3001 Berne, Switzerland.
- Institute of Virology, Vetsuisse Faculty Zurich, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland.
- Swiss Institute of Equine Medicine, Vetsuisse Faculty Berne, University of Berne, and ALP-Haras, Länggasstrasse 124, Postfach 8466, CH-3001 Berne, Switzerland.
- Institute of Virology, Vetsuisse Faculty Zurich, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland.
MeSH Terms
- Animals
- Base Sequence
- Bayes Theorem
- Bovine papillomavirus 1 / classification
- Bovine papillomavirus 1 / genetics
- Bovine papillomavirus 1 / isolation & purification
- Cattle
- Cattle Diseases / virology
- Computational Biology
- DNA, Viral / chemistry
- DNA, Viral / isolation & purification
- Equidae / virology
- Genome, Viral
- High-Throughput Nucleotide Sequencing
- Horse Diseases / virology
- Horses / virology
- Host Specificity
- Open Reading Frames
- Papillomavirus Infections / veterinary
- Papillomavirus Infections / virology
- Phylogeny
- Sequence Alignment
- Sequence Homology, Nucleic Acid
Citations
This article has been cited 7 times.- Jindra C, Hainisch EK, Rümmele A, Wolschek M, Muster T, Brandt S. Influenza virus vector iNS1 expressing bovine papillomavirus 1 (BPV1) antigens efficiently induces tumour regression in equine sarcoid patients. PLoS One 2021;16(11):e0260155.
- Jindra C, Kamjunke AK, Jones S, Brandt S. Screening for bovine papillomavirus type 13 (BPV13) in a European population of sarcoid-bearing equids. Equine Vet J 2021 Aug 30;54(4):662-9.
- Savini F, Gallina L, Prosperi A, Puleio R, Lavazza A, Di Marco P, Tumino S, Moreno A, Lelli D, Guercio A, Scagliarini A. Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily. Pathogens 2020 May 30;9(6).
- Ata EB, Mahmoud MAE, Madboli AA. Molecular detection and immunopathological examination of Deltapapillomavirus 4 in skin and udder of Egyptian cattle. Vet World 2018 Jul;11(7):915-920.
- Shehata AA, Fawzi EM, Abd El-Emam MM, Abdullah SM, Hassan W, Eldin ALAZ, Elsheikh HEM. Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt. Vet Med Int 2025;2025:9808789.
- Cutarelli A, Passantino G, Razzuoli E, Serpe F, Leonardi L, Zizzo N, Roperto S. Digital droplet PCR-based detection and quantification of ovine papillomavirus DNA from the vaginal virobiota of healthy mares. Sci Rep 2025 Mar 22;15(1):9951.
- Sobhy NM, Refaai W, Kumar R, Bottros Youssef CR, Goyal SM. Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt. Vet Med Int 2025;2025:9773642.
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