Genomic diversity among equine herpesvirus-4 field isolates.
Abstract: Infection with equine herpesvirus-4 (EHV-4) is a major cause of respiratory tract disease, equine rhinopneumonitis, in horses. Although the full sequence of EHV-4 has been reported, genomic differences among EHV-4 field isolates have not yet been characterized. In this study, the genomic diversity between 23 Japanese EHV-4 isolates was analyzed by digestion with restriction endonucleases (BamHI, BgIII, EcoRI, SacI, and SalI) and polymerase chain reaction (PCR). The restriction endonuclease digestion patterns of the EHV-4 field isolates showed distinct differences which included mobility shifts of some fragments as well as loss and/or gain of fragments. Two EHV-4 genes containing repeat sequences, ORFs 24 and 71, were amplified by PCR and the amplified fragments were compared among the field isolates. The sizes of the amplified fragments varied among epizootiologically unrelated isolates, while the fragments of related isolates had the same size. The observed genomic diversity among EHV-4 field isolates may be a useful tool for epidemiological study of equine rhinopneumonitis by EHV-4 infection.
Publication Date: 2005-07-06 PubMed ID: 15997181DOI: 10.1292/jvms.67.555Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research studies the genomic diversity found between different isolates of equine herpesvirus-4 (EHV-4), a major cause of respiratory illness in horses. The study aims to help in the epidemiological study of equine rhinopneumonitis caused by EHV-4 infection.
Research Methodology
- The scientists took 23 Japanese EHV-4 isolates and analyzed their genomic diversity through digestion with restriction endonucleases (BamHI, BgIII, EcoRI, SacI, and SalI) and polymerase chain reaction (PCR).
- The restriction endonucleases were used to digest the EHV-4 genomic DNA into fragments, the pattern of which was compared amongst different isolates.
- PCR was used to amplify two EHV-4 genes containing repeat sequences, known as ORFs 24 and 71.
- The fragments produced by the digestion and amplification processes were compared in order to identify any variations in the length of these fragments amongst different isolates.
Findings & Results
- The patterns of the DNA fragments after restriction endonuclease digestion showed distinct differences between the isolates. This included mobility shifts of some fragments, along with the loss and/or gain of other fragments. This indicates variability in the genomic makeup of different EHV-4 isolates.
- The PCR amplified fragments of the ORFs 24 and 71 genes also varied in size amongst isolates that had no epidemiological relationship. However, the fragment sizes were the same for related isolates, suggesting a genetic similarity amongst these groups.
Implications & Conclusions
- The observed genomic diversity amongst EHV-4 field isolates indicates the presence of distinct genetic differences and could potentially be used to understand the spread and evolution of the virus.
- Identifying these patterns could contribute to the epidemiological studies of equine rhinopneumonitis caused by EHV-4 infection.
- This, in turn, could be crucial in enhancing outbreak control strategies and improving treatments for affected horses.
Cite This Article
APA
Maeda K, Kai K, Matsumura T.
(2005).
Genomic diversity among equine herpesvirus-4 field isolates.
J Vet Med Sci, 67(6), 555-561.
https://doi.org/10.1292/jvms.67.555 Publication
Researcher Affiliations
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.
MeSH Terms
- Animals
- DNA Primers
- Electrophoretic Mobility Shift Assay
- Genetic Variation
- Genome, Viral
- Herpesvirus 4, Equid / genetics
- Horses / virology
- Japan
- Restriction Mapping
Citations
This article has been cited 3 times.- Izume S, Kirisawa R, Ohya K, Ohnuma A, Kimura T, Omatsu T, Katayama Y, Mizutani T, Fukushi H. The full genome sequences of 8 equine herpesvirus type 4 isolates from horses in Japan. J Vet Med Sci 2017 Jan 24;79(1):206-212.
- Thorsteinsdóttir L, Torsteinsdóttir S, Svansson V. Establishment and characterization of fetal equine kidney and lung cells with extended lifespan. Susceptibility to equine gammaherpesvirus infection and transfection efficiency. In Vitro Cell Dev Biol Anim 2016 Sep;52(8):872-7.
- Azab W, El-Sheikh A, Abdel-Gawad A. In vitro characterization of EHV-4 gG-deleted mutant. Virus Genes 2012 Feb;44(1):109-11.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
