Genomic hybrid capture assay to detect Borrelia burgdorferi: an application to diagnose neuroborreliosis in horses.
Abstract: Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for in CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the flagellin gene was negative. However, we enriched DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified in the CSF of the horse, confirming a diagnosis of neuroborreliosis.
Publication Date: 2022-07-21 PubMed ID: 35864735PubMed Central: PMC9446311DOI: 10.1177/10406387221112617Google Scholar: Lookup
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- Journal Article
Summary
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The researchers in this study designing a genomic hybrid capture assay to detect the bacterium Borrelia burgdorferi, with the goal of diagnosing neuroborreliosis in horses more effectively.
Research Context
- Neuroborreliosis is a condition affecting horses, and is caused by the bacterium Borrelia burgdorferi.
- The disease is challenging to confirm while the horse is still alive (antemortem) due to the limitations of the current diagnostic tests.
- PCR (Polymerase chain reaction) tests used to identify this bacteria in the cerebrospinal fluid (CSF) are not highly sensitive.
- The serum:CSF ELISA ratios method that examines intrathecal antibody production against Borrelia burgdorferi also lacks specificity.
Research Method and Finding
- The team examined the CSF of an adult horse showing acute neurological disorder using PCR test targeting the flagellin gene, a component of Borrelia burgdorferi, but the result was negative.
- Instead of relying on this result, the researchers took another approach. They enriched the Borrelia burgdorferi DNA in the CSF sample through a process known as nucleic acid hybrid capture.
- Following this process, they carried out a next-generation sequencing (NGS) procedure.
- As a result of this NGS process, the researchers identified the presence of Borrelia burgdorferi in the horse’s CSF.
- Therefore, the horse was correctly diagnosed with neuroborreliosis, which contradicted the initial PCR test result.
- This outcome indicates that the genomic hybrid capture assay combined with NGS could be a more effective method to detect Borrelia burgdorferi and diagnose neuroborreliosis in horses.
Cite This Article
APA
Divers TJ, Mongodin EF, Miller CB, Belgrave RL, Gardner RB, Fraser CM, Schutzer SE.
(2022).
Genomic hybrid capture assay to detect Borrelia burgdorferi: an application to diagnose neuroborreliosis in horses.
J Vet Diagn Invest, 34(5), 909-912.
https://doi.org/10.1177/10406387221112617 Publication
Researcher Affiliations
- College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
- Institute of Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
- Miller and Associates Equine Practice, Brewster, NY, USA.
- Mid-Atlantic Equine Hospital, Ringoes, NJ, USA.
- B.W. Furlong and Associates, Oldwick, NJ, USA.
- Institute of Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
- Institute of Genome Sciences, Rutgers New Jersey Medical School, Newark, NJ, USA.
MeSH Terms
- Animals
- Antibodies, Bacterial
- Borrelia burgdorferi / genetics
- Borrelia burgdorferi Group / genetics
- Enzyme-Linked Immunosorbent Assay / veterinary
- Genomics
- Horse Diseases / diagnosis
- Horses
- Lyme Disease / diagnosis
- Lyme Disease / veterinary
- Nervous System Diseases / veterinary
Grant Funding
- R43 AI114009 / NIAID NIH HHS
- R44 AI114009 / NIAID NIH HHS
- U19 AI110820 / NIAID NIH HHS
Conflict of Interest Statement
The authors declared no potential conflicts of interest with respect to the research,
authorship, and/or publication of this article.
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