Genotyping of isolates of Taylorella equigenitalis from thoroughbred brood mares in Japan.
Abstract: Profiles of the genomic DNA of 104 strains of T. equigenitalis isolated from brood mares with contagious equine metritis in Hokkaido during the breeding seasons from 1980 to 1993, as well as those of five strains (SS28, EQ56, EQ59, EQ70 and HH139) previously isolated in Japan were examined after restriction digestion and crossed-field gel electrophoresis. These profiles were essentially identical to each other and the various isolates and strains appeared to have a common genotype, designated 'genotype J', with respect to two restriction enzymes, ApaI and NotI. These results suggest a common source for all these isolates obtained over the course of more than 10 years in Japan.
Publication Date: 1995-01-01 PubMed ID: 8540238DOI: 10.1007/BF01839309Google Scholar: Lookup
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- Journal Article
Summary
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The research involves genotyping of Taylorella equigenitalis, a bacterium causing contagious equine metritis (a sexually transmitted disease in horses), isolated from thoroughbred brood mares in Japan from 1980-1993. The study suggests that the isolates over this 10 year period have a common genotype, indicating a shared origin.
Background of the Research
- The research focuses on Taylorella equigenitalis, a bacterium responsible for a sexually transmitted disease in horses, known as contagious equine metritis.
- The bacteria were isolated from thoroughbred brood mares in Japan over the breeding seasons from 1980 to 1993, plus five strains that were previously isolated in Japan.
Research Methodology
- The team conducted genotyping of the bacterial DNA. This involves characterising the genetic makeup of the organism, with an aim to identify any specific ‘markers’ or characteristics.
- Strains of T. equigenitalis were subjected to restriction digestion and crossed-field gel electrophoresis.
In restriction digestion, specific enzymes (in this case, ApaI and NotI) are used to cut DNA into smaller fragments while crossed-field electrophoresis is a technique that helps in visualising the DNA fragments.
Results and Interpretation
- Upon analysis, researchers discovered that the genomic DNA profiles of the different strains were essentially identical; all shared a common genotype, termed ‘genotype J’.
- The similarities in the genetic profiles of these bacteria suggest that they likely originated from a common source over the more than ten-year isolation period in Japan.
- This genotyping process can strengthen measures to control the spread of the disease since it suggests that the pathogen’s spread could be traced back to this common source.
Cite This Article
APA
Miyazawa T, Matsuda M, Isayama Y, Samata T, Ishida Y, Ogawa S, Takei K, Honda M, Kamada M.
(1995).
Genotyping of isolates of Taylorella equigenitalis from thoroughbred brood mares in Japan.
Vet Res Commun, 19(4), 265-271.
https://doi.org/10.1007/BF01839309 Publication
Researcher Affiliations
- Department of Biosciences, College of Environmental Health, Azabu University, Sagamihara, Japan.
MeSH Terms
- Animals
- DNA Restriction Enzymes
- DNA, Bacterial / analysis
- Electrophoresis, Gel, Pulsed-Field / veterinary
- Female
- Genotype
- Haemophilus / classification
- Haemophilus / genetics
- Haemophilus / isolation & purification
- Haemophilus Infections / epidemiology
- Haemophilus Infections / microbiology
- Haemophilus Infections / veterinary
- Horse Diseases / epidemiology
- Horse Diseases / microbiology
- Horses
- Japan / epidemiology
- Restriction Mapping
- Sexually Transmitted Diseases / epidemiology
- Sexually Transmitted Diseases / microbiology
- Sexually Transmitted Diseases / veterinary
References
This article includes 8 references
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Citations
This article has been cited 9 times.- Hrala M, Andrla P, Bosák J, Fedrová P, Mugutdinov A, Karpíšková R, Nedbalcová K, Raichová J, Faldyna M, Hořín P, Šmajs D. Whole genome sequences of nine Taylorella equigenitalis strains isolated in the Czech Republic between 1982-2021: Molecular dating suggests a common ancestor at the time of Roman Empire. PLoS One 2025;20(1):e0315946.
- Hicks J, Stuber T, Lantz K, Erdman M, Robbe-Austerman S, Huang X. Genomic diversity of Taylorella equigenitalis introduced into the United States from 1978 to 2012. PLoS One 2018;13(3):e0194253.
- Aalsburg AM, Erdman MM. Pulsed-field gel electrophoresis genotyping of Taylorella equigenitalis isolates collected in the United States from 1978 to 2010. J Clin Microbiol 2011 Mar;49(3):829-33.
- Tazumi A, Sekizuka T, Moore JE, Millar BC, Taneike I, Matsuda M. Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis. Folia Microbiol (Praha) 2008;53(6):486-92.
- Kagawa S, Nagano Y, Tazumi A, Murayama O, Millar BC, Moore JE, Matsuda M. Nucleotide sequencing and analysis of 16S rDNA and 16S-23S rDNA internal spacer region (ISR) of Taylorella equigenitalis, as an important pathogen for contagious equine metritis (CEM). Vet Res Commun 2006 May;30(4):343-55.
- Matsuda M, Tazumi A, Kagawa S, Sekizuka T, Murayama O, Moore JE, Millar BC. Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis. BMC Vet Res 2006 Jan 6;2:1.
- Kagawa S, Klein F, Corboz L, Moore JE, Murayama O, Matsuda M. Demonstration of heterogeneous genotypes of Taylorella equigenitalis isolated from horses in six European countries by pulsed-field gel electrophoresis. Vet Res Commun 2001 Oct;25(7):565-75.
- Kagawa S, Moore JE, Murayama O, Matsuda M. Comparison of the value of pulsed-field gel electrophoresis, random amplified polymorphic DNA and amplified rDNA restriction analysis for subtyping Taylorella equigenitalis. Vet Res Commun 2001 May;25(4):261-9.
- Matsuda M, Miyazawa T, Moore JE, Buckley TC, Thomas LA. Molecular genotyping by pulsed-field gel electrophoresis of restricted genomic DNA of strains of Taylorella equigenitalis isolated in Ireland and in the United States. Vet Res Commun 1998 Jun;22(4):217-24.
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